BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	554,868	G→T	E147* (GAA→TAA)	sfmC →	putative periplasmic pilus chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	554,868	0	G	T	100.0%	742.8 / NA	225	E147* (GAA→TAA)	sfmC	putative periplasmic pilus chaperone
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (101/124); total (101/124)

Only 100 of 231 total aligned reads displayed.

CCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAAGAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCTGCACCCAGA  >  CP009273/554721‑555014

ccGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATaa                                                                                                                                                  <  2:3217596/150‑1 (MQ=255)

 cGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACaataat                                                                                                                                                 <  2:1725085/150‑1 (MQ=255)

 cGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACaataat                                                                                                                                                 >  1:515779/1‑150 (MQ=255)

 cGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACaataat                                                                                                                                                 >  1:1826350/1‑150 (MQ=255)

  gCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAAtt                                                                                                                                                >  2:95468/1‑150 (MQ=255)

     ggCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTAcc                                                                                                                                             >  2:3304459/1‑150 (MQ=255)

       cagcagATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCg                                                                                                                                           <  2:1219608/150‑1 (MQ=255)

        agcagATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGc                                                                                                                                          >  1:3140714/1‑150 (MQ=255)

          cagATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAg                                                                                                                                        >  2:2904291/1‑150 (MQ=255)

           agATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGa                                                                                                                                       >  2:380763/1‑150 (MQ=255)

               cGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAgcg                                                                                                                                   <  1:3026647/150‑1 (MQ=255)

                 tGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGcc                                                                                                                                 <  1:3426854/150‑1 (MQ=255)

                  gAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCg                                                                                                                                <  1:2786765/150‑1 (MQ=255)

                  gAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCg                                                                                                                                <  1:1881587/150‑1 (MQ=255)

                    gTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGa                                                                                                                              >  1:3115716/1‑150 (MQ=255)

                     tctcTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGac                                                                                                                             <  1:1641648/150‑1 (MQ=255)

                       tctGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGacac                                                                                                                           <  1:1476837/150‑1 (MQ=255)

                       tctGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGacac                                                                                                                           <  1:1253995/150‑1 (MQ=255)

                               ggATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGt                                                                                                                   <  2:2004973/150‑1 (MQ=255)

                               ggATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGt                                                                                                                   <  1:2767130/150‑1 (MQ=255)

                                gATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGtt                                                                                                                  >  2:1450694/1‑150 (MQ=255)

                                   gAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTc                                                                                                               <  1:2532630/150‑1 (MQ=255)

                                    aaTGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTcg                                                                                                              >  2:2977136/1‑150 (MQ=255)

                                     aTGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTcgc                                                                                                             >  2:1877955/1‑150 (MQ=255)

                                       gTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGa                                                                                                           >  2:1799741/1‑150 (MQ=255)

                                          aaGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATcc                                                                                                        >  1:2056745/1‑150 (MQ=255)

                                           aGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCg                                                                                                       <  1:3249201/150‑1 (MQ=255)

                                           aGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCg                                                                                                       <  2:1856991/150‑1 (MQ=255)

                                           aGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCg                                                                                                       <  1:385385/150‑1 (MQ=255)

                                           aGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCg                                                                                                       <  2:3571180/150‑1 (MQ=255)

                                           aGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCg                                                                                                       >  1:904471/1‑150 (MQ=255)

                                            gACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCgg                                                                                                      <  2:3381475/150‑1 (MQ=255)

                                               gATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTaa                                                                                                   >  2:3085760/1‑150 (MQ=255)

                                                 tCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACt                                                                                                 <  1:2428229/150‑1 (MQ=255)

                                                  cccTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACta                                                                                                <  1:1401130/150‑1 (MQ=255)

                                                    cTTCTGTAGATAAAAATGCATTGATCGGCAGGAATGTTTTGCAACTTGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACtata                                                                                              <  2:444556/150‑1 (MQ=255)

                                                       cGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCa                                                                                           <  1:3716012/150‑1 (MQ=255)

                                                         gTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAAt                                                                                         >  2:3244487/1‑150 (MQ=255)

                                                           aGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGt                                                                                       <  1:3040398/150‑1 (MQ=255)

                                                            gATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGtt                                                                                      <  2:154427/150‑1 (MQ=255)

                                                            gATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGtt                                                                                      <  2:3738252/150‑1 (MQ=255)

                                                                aaaaTGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATa                                                                                  <  2:2862868/150‑1 (MQ=255)

                                                                 aaaTGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATaa                                                                                 >  2:276267/1‑150 (MQ=255)

                                                                   aTGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATc                                                                               <  1:2939336/150‑1 (MQ=255)

                                                                     gCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCa                                                                             <  2:2427729/150‑1 (MQ=255)

                                                                      cATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCAt                                                                            <  1:2611412/150‑1 (MQ=255)

                                                                      cATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCAt                                                                            >  1:44399/1‑150 (MQ=255)

                                                                       aTTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATc                                                                           >  1:1050980/1‑150 (MQ=255)

                                                                       aTTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATc                                                                           >  1:2007092/1‑150 (MQ=255)

                                                                           aaCGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCt                                                                       >  2:2607269/1‑150 (MQ=255)

                                                                             cGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCttt                                                                     <  2:2091488/150‑1 (MQ=255)

                                                                             cGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCttt                                                                     <  2:3140714/150‑1 (MQ=255)

                                                                             cGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCttt                                                                     <  1:1383626/150‑1 (MQ=255)

                                                                              ggCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCtttt                                                                    >  2:2230999/1‑150 (MQ=255)

                                                                              ggCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCtttt                                                                    <  1:2680920/150‑1 (MQ=255)

                                                                                cAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTa                                                                  >  1:2246869/1‑150 (MQ=255)

                                                                                 aGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTAt                                                                 >  1:174100/1‑150 (MQ=255)

                                                                                     aTGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCa                                                             <  1:1065843/150‑1 (MQ=255)

                                                                                      tGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCAc                                                            >  2:3460879/1‑150 (MQ=255)

                                                                                       gTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCAcc                                                           >  2:2045688/1‑150 (MQ=255)

                                                                                        ttttGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCAccc                                                          <  2:3372042/150‑1 (MQ=255)

                                                                                        ttttGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCAccc                                                          <  2:3750782/150‑1 (MQ=255)

                                                                                           tGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTgg                                                       <  1:3589444/150‑1 (MQ=255)

                                                                                           tGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTgg                                                       <  1:2348363/150‑1 (MQ=255)

                                                                                            gCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGt                                                      <  2:1796194/150‑1 (MQ=255)

                                                                                             cAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGtt                                                     >  2:3574947/1‑150 (MQ=255)

                                                                                               aCTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTaa                                                   <  2:904471/150‑1 (MQ=255)

                                                                                                  ggCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACtt                                                >  2:1080516/1‑150 (MQ=255)

                                                                                                   gCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTa                                               <  1:1037410/150‑1 (MQ=255)

                                                                                                    cGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCCGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGGAACTATATCAATGTTCATAATCCCTCACCTTTGTATGTCACCCTGGTTAACTTAc                                              >  2:3386654/1‑150 (MQ=255)

                                                                                                     gATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGGAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACa                                             <  2:5800/150‑1 (MQ=255)

                                                                                                     gATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACa                                             >  2:137287/1‑150 (MQ=255)

                                                                                                      aTTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACaa                                            >  1:3155361/1‑150 (MQ=255)

                                                                                                          tATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTgg                                        <  1:3349406/150‑1 (MQ=255)

                                                                                                          tATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTgg                                        <  2:3170094/150‑1 (MQ=255)

                                                                                                          tATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTgg                                        <  1:641594/150‑1 (MQ=255)

                                                                                                           aTCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTggg                                       <  2:702909/150‑1 (MQ=255)

                                                                                                             cgcgCATGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCa                                     >  1:375867/1‑150 (MQ=255)

                                                                                                                  aTGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCaa                                >  2:2714185/1‑150 (MQ=255)

                                                                                                                  aTGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCaa                                <  2:515779/150‑1 (MQ=255)

                                                                                                                  aTGAAATTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCaa                                <  2:2402432/150‑1 (MQ=255)

                                                                                                                     aaaTTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAg                             <  2:788847/150‑1 (MQ=255)

                                                                                                                     aaaTTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAg                             <  2:1263793/150‑1 (MQ=255)

                                                                                                                      aaTTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGt                            >  1:2288469/1‑150 (MQ=255)

                                                                                                                      aaTTATTTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGt                            >  1:1983867/1‑150 (MQ=255)

                                                                                                                       attattTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGtt                           >  1:2976390/1‑150 (MQ=255)

                                                                                                                          attTCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTggg                        <  2:2269374/150‑1 (MQ=255)

                                                                                                                            ttCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTggggc                      >  2:3741738/1‑149 (MQ=255)

                                                                                                                            ttCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGa                      <  2:3325280/150‑1 (MQ=255)

                                                                                                                            ttCTCCGTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACCTACAAGTGGGCAGCCAAAAGTTGGGGa                      <  2:1427285/150‑1 (MQ=255)

                                                                                                                                  gTCCAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTa                >  1:2918396/1‑150 (MQ=255)

                                                                                                                                    ccAATTCAATTACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATg              >  1:1598827/1‑150 (MQ=255)

                                                                                                                                       attcaattACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCt           >  2:3647426/1‑150 (MQ=255)

                                                                                                                                       attcaattACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCt           >  1:887246/1‑150 (MQ=255)

                                                                                                                                       attcaattACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCt           >  1:2556393/1‑150 (MQ=255)

                                                                                                                                       attcaattACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGGTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCt           <  2:662527/150‑1 (MQ=255)

                                                                                                                                        ttctattACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCTg          <  1:213867/150‑1 (MQ=255)

                                                                                                                                         tcaattACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCTGc         >  1:845058/1‑150 (MQ=255)

                                                                                                                                             ttACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCTGCAccc     >  1:114003/1‑150 (MQ=255)

                                                                                                                                             ttACAATAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCTGCAccc     >  1:2507016/1‑150 (MQ=255)

CCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAAGAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGATCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTACAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCTGCACCCAGA  >  CP009273/554721‑555014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`