Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 5,144 G→A intergenic (+124/‑90) thrC → / → yaaX L‑threonine synthase/DUF2502 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092735,1440GA100.0% 682.5 / NA 205intergenic (+124/‑90)thrC/yaaXL‑threonine synthase/DUF2502 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (117/88);  total (117/88)

Only 100 of 207 total aligned reads displayed.

AAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATG  >  CP009273/4997‑5290
                                                                                                                                                   |                                                                                                                                                  
aaaTTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAAt                                                                                                                                                  >  1:2870517/1‑150 (MQ=255)
 aaTTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAAtt                                                                                                                                                 <  2:3726452/150‑1 (MQ=255)
  aTTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTa                                                                                                                                                <  2:1009105/150‑1 (MQ=255)
       tgatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGAtt                                                                                                                                           >  2:3482087/1‑150 (MQ=255)
         atgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGc                                                                                                                                         <  1:1604963/150‑1 (MQ=255)
           gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCgg                                                                                                                                       >  1:919265/1‑150 (MQ=255)
             atcatcAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGgag                                                                                                                                     >  1:1795724/1‑150 (MQ=255)
                atcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAAt                                                                                                                                  >  1:2740918/1‑150 (MQ=255)
                  caGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATaa                                                                                                                                <  1:2203370/150‑1 (MQ=255)
                   aGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATaac                                                                                                                               <  1:3347838/150‑1 (MQ=255)
                    gTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATaaca                                                                                                                              >  2:2580029/1‑150 (MQ=255)
                    gTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATaaca                                                                                                                              <  2:1966137/150‑1 (MQ=255)
                           cTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAAccgccg                                                                                                                       >  1:1492231/1‑150 (MQ=255)
                           cTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAAccgccg                                                                                                                       >  1:1909546/1‑150 (MQ=255)
                            tATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGt                                                                                                                      >  2:2618781/1‑150 (MQ=255)
                            tATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGt                                                                                                                      >  2:1149554/1‑150 (MQ=255)
                             aTTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGtt                                                                                                                     >  2:3375249/1‑150 (MQ=255)
                               tCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTtct                                                                                                                   >  2:558128/1‑150 (MQ=255)
                                cATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTtctc                                                                                                                  >  1:902572/1‑150 (MQ=255)
                                 aTTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCa                                                                                                                 >  1:2759881/1‑150 (MQ=255)
                                   tATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATc                                                                                                               >  2:211895/1‑150 (MQ=255)
                                    aTCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCg                                                                                                              >  2:1736758/1‑150 (MQ=255)
                                     tctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGa                                                                                                             <  2:3652338/150‑1 (MQ=255)
                                     tctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGa                                                                                                             >  1:2642427/1‑150 (MQ=255)
                                      ctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAg                                                                                                            <  1:3252718/150‑1 (MQ=255)
                                      ctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAg                                                                                                            <  2:2325087/150‑1 (MQ=255)
                                       tcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGt                                                                                                           >  1:3316042/1‑150 (MQ=255)
                                       tcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAAATCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGt                                                                                                           >  1:1676974/1‑150 (MQ=255)
                                         aaTCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTaa                                                                                                         <  1:2449890/150‑1 (MQ=255)
                                          aTCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAAt                                                                                                        >  2:1165362/1‑150 (MQ=255)
                                          aTCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAAt                                                                                                        <  2:797456/150‑1 (MQ=255)
                                            cAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAAtct                                                                                                      >  1:123666/1‑150 (MQ=255)
                                             aGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAAtctc                                                                                                     >  1:2987937/1‑150 (MQ=255)
                                              ggCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTcc                                                                                                    <  1:832324/150‑1 (MQ=255)
                                                ccGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCgg                                                                                                  <  2:2788762/150‑1 (MQ=255)
                                                ccGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCgg                                                                                                  <  1:3589014/150‑1 (MQ=255)
                                                  gggTTTGCTTTTATGCAGCCCTGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                <  2:2231923/150‑1 (MQ=255)
                                                  gggTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                >  2:1874870/1‑150 (MQ=255)
                                                  gggTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                >  1:3553413/1‑150 (MQ=255)
                                                   ggTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGata                                                                                               >  2:1306309/1‑150 (MQ=255)
                                                   ggTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGata                                                                                               <  2:855103/150‑1 (MQ=255)
                                                     tttGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATc                                                                                             <  2:1120950/150‑1 (MQ=255)
                                                          ttttATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAccc                                                                                        <  1:2270868/150‑1 (MQ=255)
                                                            ttATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCAt                                                                                      >  1:1554447/1‑150 (MQ=255)
                                                                 cAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACgg                                                                                 <  1:1475745/150‑1 (MQ=255)
                                                                    cccGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCa                                                                              >  2:2908672/1‑150 (MQ=255)
                                                                     ccGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa                                                                             <  1:24802/150‑1 (MQ=255)
                                                                      cGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAAt                                                                            <  2:1416009/150‑1 (MQ=255)
                                                                       ggCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATg                                                                           <  2:1900269/150‑1 (MQ=255)
                                                                        gCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGa                                                                          >  2:3582610/1‑150 (MQ=255)
                                                                        gCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGa                                                                          >  2:2131446/1‑150 (MQ=255)
                                                                        gCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGa                                                                          >  2:2131517/1‑150 (MQ=255)
                                                                         cTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGAt                                                                         >  2:245738/1‑150 (MQ=255)
                                                                         cTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGAt                                                                         >  2:1309053/1‑150 (MQ=255)
                                                                         cTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGAt                                                                         <  2:2277716/150‑1 (MQ=255)
                                                                              ttATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAg                                                                    >  2:2544219/1‑150 (MQ=255)
                                                                                   aagaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTa                                                               >  2:3038138/1‑150 (MQ=255)
                                                                                    agaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTaa                                                              >  1:1064478/1‑150 (MQ=255)
                                                                                    agaaATTATGGAGAAAAATGACAGGG‑AAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAc                                                             <  1:3342580/150‑1 (MQ=255)
                                                                                      aaaTTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAcc                                                            >  1:3464459/1‑150 (MQ=255)
                                                                                       aaTTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCt                                                           <  1:2498979/150‑1 (MQ=255)
                                                                                       aaTTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCt                                                           >  2:2851675/1‑150 (MQ=255)
                                                                                         ttATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt                                                         >  2:40020/1‑150 (MQ=255)
                                                                                            tGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaa                                                      <  2:2666286/150‑1 (MQ=255)
                                                                                             ggAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaa                                                     >  2:864931/1‑150 (MQ=255)
                                                                                                gaAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAg                                                  <  1:211895/150‑1 (MQ=255)
                                                                                                 aaaaaTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGa                                                 <  1:2901455/150‑1 (MQ=255)
                                                                                                  aaaaTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGAt                                                >  1:3079490/1‑150 (MQ=255)
                                                                                                       gACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAAt                                           <  1:2450905/150‑1 (MQ=255)
                                                                                                       gACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAAt                                           >  1:969020/1‑150 (MQ=255)
                                                                                                         cAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCt                                         <  1:3355918/150‑1 (MQ=255)
                                                                                                         cAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCt                                         <  2:2870517/150‑1 (MQ=255)
                                                                                                            ggAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATc                                      >  2:274282/1‑150 (MQ=255)
                                                                                                             gAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCg                                     >  1:3765823/1‑150 (MQ=255)
                                                                                                             gAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCg                                     >  1:1348156/1‑150 (MQ=255)
                                                                                                                aaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTAc                                  >  2:1352691/1‑150 (MQ=255)
                                                                                                                 aaGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACt                                 >  2:1835981/1‑150 (MQ=255)
                                                                                                                  aGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTc                                >  1:1140856/1‑150 (MQ=255)
                                                                                                                  aGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTc                                <  2:1170003/150‑1 (MQ=255)
                                                                                                                   ggAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCg                               >  1:430285/1‑150 (MQ=255)
                                                                                                                       aaaTTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACt                           >  1:1759132/1‑150 (MQ=255)
                                                                                                                            ctcAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTccc                      >  1:249058/1‑150 (MQ=255)
                                                                                                                             tcAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCt                     <  1:1306309/150‑1 (MQ=255)
                                                                                                                             tcAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCt                     <  1:3573453/150‑1 (MQ=255)
                                                                                                                             tcAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCt                     <  1:371470/150‑1 (MQ=255)
                                                                                                                              cAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTg                    <  2:1492231/150‑1 (MQ=255)
                                                                                                                               aaTAAATGCGGTAACTTAGAAATTAGTATTGCGGAGAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACGCCCACTTACCCTgg                   >  2:3486185/1‑150 (MQ=255)
                                                                                                                               aaTAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTgg                   <  2:123666/150‑1 (MQ=255)
                                                                                                                                aTAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGt                  >  1:1167114/1‑150 (MQ=255)
                                                                                                                                  aaaTGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTc                <  2:730477/150‑1 (MQ=255)
                                                                                                                                   aaTGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCt               <  2:2759881/150‑1 (MQ=255)
                                                                                                                                      gCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGt            <  2:2016393/150‑1 (MQ=255)
                                                                                                                                        ggTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCg          <  1:629322/150‑1 (MQ=255)
                                                                                                                                        ggTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCg          <  2:681142/150‑1 (MQ=255)
                                                                                                                                            aCTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTcc      >  2:941291/1‑150 (MQ=255)
                                                                                                                                             cTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTccc     <  2:332684/150‑1 (MQ=255)
                                                                                                                                              ttAGAACTTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTCATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCa    <  2:2490080/150‑1 (MQ=255)
                                                                                                                                               tAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAt   >  2:2157606/1‑150 (MQ=255)
                                                                                                                                               tAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAt   >  2:2352709/1‑150 (MQ=255)
                                                                                                                                                agaaATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATg  <  1:3327712/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATG  >  CP009273/4997‑5290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑