BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	614,726	G→T	A339A (GCG→GCT)	fepE →	regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	614,726	0	G	T	100.0%	690.1 / NA	212	A339A (GCG→GCT)	fepE	regulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (105/107); total (105/107)

Only 100 of 213 total aligned reads displayed.

GTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCC  >  CP009273/614580‑614872

  tGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCtattat                                                                                                                                               >  1:2521485/1‑150 (MQ=255)

         cTGAACGGTGAATTACGAAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATc                                                                                                                                        <  1:3530987/150‑1 (MQ=255)

           gAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCNt                                                                                                                                      <  1:1967968/150‑1 (MQ=255)

            aaCGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCtt                                                                                                                                     >  1:27267/1‑150 (MQ=255)

              cGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTc                                                                                                                                   >  1:1053801/1‑150 (MQ=255)

               ggTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTcc                                                                                                                                  <  1:527768/150‑1 (MQ=255)

               ggTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTcc                                                                                                                                  <  2:2027774/150‑1 (MQ=255)

               ggTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTcc                                                                                                                                  <  2:2658087/150‑1 (MQ=255)

                gTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCcg                                                                                                                                 <  2:2612725/150‑1 (MQ=255)

                 tGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCcgc                                                                                                                                >  1:425829/1‑150 (MQ=255)

                 tGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCcgc                                                                                                                                >  1:868866/1‑150 (MQ=255)

                  gAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCcgcg                                                                                                                               <  2:952000/150‑1 (MQ=255)

                  gAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCcgcg                                                                                                                               <  1:706749/150‑1 (MQ=255)

                   aaTTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGt                                                                                                                              >  1:1494767/1‑150 (MQ=255)

                      tACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGa                                                                                                                           >  2:2757134/1‑150 (MQ=255)

                      tACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGa                                                                                                                           >  1:1652273/1‑150 (MQ=255)

                       aCGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGAt                                                                                                                          <  1:2087571/150‑1 (MQ=255)

                        cGTAATCGTCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATc                                                                                                                         <  2:3209438/150‑1 (MQ=255)

                        cGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATc                                                                                                                         <  1:2888060/150‑1 (MQ=255)

                        cGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATc                                                                                                                         <  1:2712663/150‑1 (MQ=255)

                             tCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATcggcgg                                                                                                                    <  2:750675/150‑1 (MQ=255)

                             tCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATcggcgg                                                                                                                    >  2:3467538/1‑150 (MQ=255)

                              cGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCggg                                                                                                                   <  1:1341024/150‑1 (MQ=255)

                                  aGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGAtgg                                                                                                               >  1:3462960/1‑150 (MQ=255)

                                    tATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGAtggtg                                                                                                             >  1:479957/1‑150 (MQ=255)

                                     aTCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGAtggtgg                                                                                                            >  2:3046702/1‑150 (MQ=255)

                                         tGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTtg                                                                                                        >  2:3197972/1‑150 (MQ=255)

                                          gTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTtgt                                                                                                       <  1:424864/150‑1 (MQ=255)

                                                   ttAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTg                                                                                              <  1:784335/150‑1 (MQ=255)

                                                     aaCAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCt                                                                                            >  1:2138704/1‑150 (MQ=255)

                                                     aaCAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCt                                                                                            >  2:839880/1‑150 (MQ=255)

                                                     aaCAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCt                                                                                            >  1:3512735/1‑150 (MQ=255)

                                                      aCAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCTCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTg                                                                                           >  1:2259427/1‑150 (MQ=255)

                                                      aCAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTg                                                                                           <  1:1603334/150‑1 (MQ=255)

                                                         aaaGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTg                                                                                        >  2:2645864/1‑150 (MQ=255)

                                                           aGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTTAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTgcg                                                                                      <  2:3208132/150‑1 (MQ=255)

                                                           aGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTgcg                                                                                      >  2:895430/1‑150 (MQ=255)

                                                           aGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTgcg                                                                                      >  1:1910652/1‑150 (MQ=255)

                                                           aGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTgcg                                                                                      <  1:2740630/150‑1 (MQ=255)

                                                             cacaTGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCt                                                                                    <  1:3754801/150‑1 (MQ=255)

                                                             cacaTGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCt                                                                                    >  1:2210382/1‑150 (MQ=255)

                                                                aTGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATg                                                                                 <  2:2312330/150‑1 (MQ=255)

                                                                aTGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATg                                                                                 <  1:391233/150‑1 (MQ=255)

                                                                       cGATGTGAATTTTACGCCGTTTAAATATAAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGc                                                                          <  1:1267233/150‑1 (MQ=255)

                                                                           gtgAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATcc                                                                      >  1:588158/1‑150 (MQ=255)

                                                                            tgAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCa                                                                     >  1:3064707/1‑150 (MQ=255)

                                                                              aaTTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGa                                                                   >  1:656042/1‑150 (MQ=255)

                                                                               aTTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGaa                                                                  >  2:926081/1‑150 (MQ=255)

                                                                                   tACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACa                                                              <  1:962926/150‑1 (MQ=255)

                                                                                       ccGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGAt                                                          >  1:3792766/1‑150 (MQ=255)

                                                                                        cGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATg                                                         >  2:2596399/1‑150 (MQ=255)

                                                                                        cGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATg                                                         >  2:3273092/1‑150 (MQ=255)

                                                                                         gTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGc                                                        >  2:1394074/1‑150 (MQ=255)

                                                                                          tttAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGcc                                                       >  2:2990716/1‑150 (MQ=255)

                                                                                          tttAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGcc                                                       <  1:3657970/150‑1 (MQ=255)

                                                                                           ttAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCa                                                      <  2:2722550/150‑1 (MQ=255)

                                                                                             aaaTATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCatg                                                    <  1:1349494/150‑1 (MQ=255)

                                                                                             aaaTATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCatg                                                    >  2:637463/1‑150 (MQ=255)

                                                                                              aaTATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCatga                                                   <  1:2156821/150‑1 (MQ=255)

                                                                                                tatCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCatgatg                                                 >  1:3008605/1‑150 (MQ=255)

                                                                                                tatCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCatgatg                                                 <  2:1383302/150‑1 (MQ=255)

                                                                                                tatCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCatgatg                                                 >  1:1107755/1‑150 (MQ=255)

                                                                                                 atCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATgg                                                <  2:1428977/150‑1 (MQ=255)

                                                                                                   cAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCa                                              <  1:2782179/150‑1 (MQ=255)

                                                                                                   cAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCa                                              <  1:975435/150‑1 (MQ=255)

                                                                                                    aGTTATGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAg                                             >  1:177393/1‑150 (MQ=255)

                                                                                                    aGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCCTGATGGCAg                                             >  1:563293/1‑150 (MQ=255)

                                                                                                    aGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAg                                             >  2:234584/1‑150 (MQ=255)

                                                                                                     gTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGa                                            >  1:3423519/1‑150 (MQ=255)

                                                                                                         aGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCAc                                        <  2:2185201/150‑1 (MQ=255)

                                                                                                         aGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCAc                                        <  2:479957/150‑1 (MQ=255)

                                                                                                         aGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCAc                                        >  1:2385940/1‑150 (MQ=255)

                                                                                                         aGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCAc                                        <  2:2788351/150‑1 (MQ=255)

                                                                                                           cccGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACtt                                      <  2:513325/150‑1 (MQ=255)

                                                                                                            ccGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTa                                     >  1:332117/1‑150 (MQ=255)

                                                                                                              gTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGt                                   >  2:1816992/1‑150 (MQ=255)

                                                                                                              gTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGt                                   <  2:368521/150‑1 (MQ=255)

                                                                                                                cATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGttt                                 <  1:329369/150‑1 (MQ=255)

                                                                                                                cATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGttt                                 >  1:2305759/1‑150 (MQ=255)

                                                                                                                cATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGttt                                 >  2:1218639/1‑150 (MQ=255)

                                                                                                                 aTTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTa                                >  2:3358711/1‑150 (MQ=255)

                                                                                                                 aTTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTa                                >  1:2827261/1‑150 (MQ=255)

                                                                                                                   tGCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAAc                              >  2:1627010/1‑150 (MQ=255)

                                                                                                                    gCCAGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACt                             >  2:3060026/1‑150 (MQ=255)

                                                                                                                       aGTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTAtt                          >  2:535878/1‑150 (MQ=255)

                                                                                                                        gTGAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTAttt                         >  2:3058829/1‑150 (MQ=255)

                                                                                                                          gAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGc                       >  1:1174963/1‑150 (MQ=255)

                                                                                                                          gAAAAAAGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGc                       >  1:1175400/1‑150 (MQ=255)

                                                                                                                            aaaaaGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCgg                     >  2:619478/1‑150 (MQ=255)

                                                                                                                             aaaaGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGt                    >  2:3734781/1‑150 (MQ=255)

                                                                                                                                aGACGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGa                 <  2:1155126/150‑1 (MQ=255)

                                                                                                                                  aCGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAg               <  2:3550682/150‑1 (MQ=255)

                                                                                                                                   cGGTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAgg              >  1:1819444/1‑150 (MQ=255)

                                                                                                                                    ggTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGt             <  1:1300148/150‑1 (MQ=255)

                                                                                                                                    ggTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGt             <  1:3660956/150‑1 (MQ=255)

                                                                                                                                     gTCCGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTg            <  1:3616974/150‑1 (MQ=255)

                                                                                                                                       ccGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGcc          <  1:850670/150‑1 (MQ=255)

                                                                                                                                        cGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCg         >  2:1800204/1‑150 (MQ=255)

                                                                                                                                        cGGGTAAGGCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCg         >  1:1390288/1‑150 (MQ=255)

                                                                                                                                               ggCTATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTcc  <  1:3197972/150‑1 (MQ=255)

GTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCC  >  CP009273/614580‑614872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`