BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	647,422	G→T	intergenic (‑110/‑269)	citC ← / → citA	[citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	647,422	0	G	T	100.0%	729.0 / NA	220	intergenic (‑110/‑269)	citC/citA	[citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (108/112); total (108/112)

Only 100 of 221 total aligned reads displayed.

GAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT  >  CP009273/647275‑647568

 aaCGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGta                                                                                                                                                  >  1:1296781/1‑150 (MQ=255)

 aaCGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGta                                                                                                                                                  >  1:3619088/1‑150 (MQ=255)

    gTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTatat                                                                                                                                               >  2:3003333/1‑150 (MQ=255)

       ttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCtt                                                                                                                                            >  2:1367478/1‑150 (MQ=255)

        tACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCttt                                                                                                                                           >  2:2073436/1‑150 (MQ=255)

        tACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCttt                                                                                                                                           <  1:3407425/150‑1 (MQ=255)

            cgGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAATGGGGTGATCGATGTATATCTTTTtct                                                                                                                                       >  2:3153575/1‑150 (MQ=255)

             gggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtctc                                                                                                                                      <  2:736158/150‑1 (MQ=255)

             gggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtctc                                                                                                                                      >  2:2023277/1‑150 (MQ=255)

                 gAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCttt                                                                                                                                  >  1:518278/1‑150 (MQ=255)

                 gAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCttt                                                                                                                                  <  1:2632873/150‑1 (MQ=255)

                  aaaaTATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTc                                                                                                                                 <  1:1057941/150‑1 (MQ=255)

                   aaaTATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCg                                                                                                                                >  1:3743493/1‑150 (MQ=255)

                      tatCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCta                                                                                                                             <  1:2470832/150‑1 (MQ=255)

                       atCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCtat                                                                                                                            >  1:3589982/1‑150 (MQ=255)

                       atCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCtat                                                                                                                            <  2:1938063/150‑1 (MQ=255)

                       atCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCtat                                                                                                                            >  1:2791472/1‑150 (MQ=255)

                           ttGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACAt                                                                                                                        <  2:3425761/150‑1 (MQ=255)

                           ttGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACAt                                                                                                                        <  2:3425596/150‑1 (MQ=255)

                              ccGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCt                                                                                                                     <  2:3043590/150‑1 (MQ=255)

                               cGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTa                                                                                                                    <  1:701176/150‑1 (MQ=255)

                                        taaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑tt                                                                                                          <  2:3619088/150‑1 (MQ=255)

                                        taaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑tt                                                                                                          <  1:2940676/150‑1 (MQ=255)

                                         aaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑ttt                                                                                                         <  2:1101229/150‑1 (MQ=255)

                                         aaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑ttt                                                                                                         >  1:1750342/1‑150 (MQ=255)

                                         aaataGTATCCAGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑ttt                                                                                                         <  1:314132/150‑1 (MQ=255)

                                          aataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTc                                                                                                        <  1:2521961/150‑1 (MQ=255)

                                          aataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTc                                                                                                        >  2:1421055/1‑150 (MQ=255)

                                            taGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCgg                                                                                                      <  2:2604680/150‑1 (MQ=255)

                                              gTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCg                                                                                                    >  2:3302675/1‑150 (MQ=255)

                                               tATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGt                                                                                                   >  1:2743584/1‑150 (MQ=255)

                                               tATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGt                                                                                                   <  2:18054/150‑1 (MQ=255)

                                                aaccTGAAGGTTCATGTTGTTATCGATTTGCAACTAATGTTGTTCAATGTTGCAAACTGATAACATATTATTTACACTTGGTAGAAAGTGGGTGATCAATTTATATCTTTTTCTCCTTACGATATACAACCTAAGCAGAA‑ATTCgtagat                                                                                                  >  1:3172038/3‑145 (MQ=255)

                                                aTCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTg                                                                                                  >  1:3697447/1‑150 (MQ=255)

                                                aTCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTATTATTTTCACTTGGGAGAAAGGAGGTGATCGATGTATATATTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTg                                                                                                  >  1:1130545/1‑150 (MQ=255)

                                                 tCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGa                                                                                                 >  2:3368786/1‑150 (MQ=255)

                                                  ccTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGaa                                                                                                >  2:2246807/1‑150 (MQ=255)

                                                   cTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTTCGGCGTGaa                                                                                                >  2:3729866/1‑150 (MQ=255)

                                                   cTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGaaa                                                                                               >  2:3671570/1‑150 (MQ=255)

                                                    tGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAAt                                                                                              >  2:1816117/1‑150 (MQ=255)

                                                     gAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAAtt                                                                                             <  1:2640315/150‑1 (MQ=255)

                                                      aaGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAAttt                                                                                            <  1:417810/150‑1 (MQ=255)

                                                      aaGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAAttt                                                                                            >  2:1591291/1‑150 (MQ=255)

                                                      aaGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAAttt                                                                                            <  2:1860805/150‑1 (MQ=255)

                                                       aGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAAtttt                                                                                           >  1:1013318/1‑150 (MQ=255)

                                                       aGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAAtttt                                                                                           <  1:1653731/150‑1 (MQ=255)

                                                       aGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAAtttt                                                                                           >  1:2894574/1‑150 (MQ=255)

                                                             aTGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGattta                                                                                     <  1:3251269/150‑1 (MQ=255)

                                                             aTGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGattta                                                                                     >  1:736127/1‑150 (MQ=255)

                                                              tgttgtTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGatttat                                                                                    <  2:2677299/150‑1 (MQ=255)

                                                                    tATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTcaca                                                                              >  1:1812537/1‑150 (MQ=255)

                                                                     aTCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACAt                                                                             <  2:1973518/150‑1 (MQ=255)

                                                                        gATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATaga                                                                          <  1:762284/150‑1 (MQ=255)

                                                                         aTTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATagag                                                                         <  2:2370258/150‑1 (MQ=255)

                                                                         aTTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATagag                                                                         >  2:2713243/1‑150 (MQ=255)

                                                                         aTTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATagag                                                                         >  1:20209/1‑150 (MQ=255)

                                                                            tGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTa                                                                      <  1:168398/150‑1 (MQ=255)

                                                                            tGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTa                                                                      <  1:2078504/150‑1 (MQ=255)

                                                                                aCGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTgg                                                                  >  1:3436784/1‑150 (MQ=255)

                                                                                aCGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTgg                                                                  >  1:1539717/1‑150 (MQ=255)

                                                                                 cGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGt                                                                 >  1:3517988/1‑150 (MQ=255)

                                                                                  gAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtt                                                                <  2:3743493/150‑1 (MQ=255)

                                                                                  gAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtt                                                                >  1:91912/1‑150 (MQ=255)

                                                                                      gttgttCAATGTTGCTAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGttttct                                                            <  2:2168904/150‑3 (MQ=255)

                                                                                        tgttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctat                                                          >  2:3462514/1‑150 (MQ=255)

                                                                                        tgttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctat                                                          <  2:1198926/150‑1 (MQ=255)

                                                                                          ttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTAATTTATTTCACATAAATTTAGTGGTTTtctattt                                                        >  1:3059472/1‑150 (MQ=255)

                                                                                            cAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttat                                                      >  2:265267/1‑150 (MQ=255)

                                                                                                 ttGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAAt                                                 <  2:2420074/150‑1 (MQ=255)

                                                                                                 ttGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAAt                                                 <  1:425412/150‑1 (MQ=255)

                                                                                                  tGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATg                                                <  2:1291886/150‑1 (MQ=255)

                                                                                                    cAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAt                                              >  1:1397560/1‑150 (MQ=255)

                                                                                                     aaaCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAtt                                             >  1:2535144/1‑150 (MQ=255)

                                                                                                      aaCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAttt                                            <  2:60992/150‑1 (MQ=255)

                                                                                                      aaCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAttt                                            >  1:1407627/1‑150 (MQ=255)

                                                                                                      aaCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAttt                                            <  2:1395476/150‑1 (MQ=255)

                                                                                                        cTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTa                                          <  1:2215094/150‑1 (MQ=255)

                                                                                                         tGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTaa                                         >  2:1401584/1‑150 (MQ=255)

                                                                                                         tGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTaa                                         >  2:2757882/1‑150 (MQ=255)

                                                                                                             aaCCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGttt                                     >  2:2783866/1‑150 (MQ=255)

                                                                                                                   ttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaat                               <  2:2254251/150‑1 (MQ=255)

                                                                                                                   ttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaat                               <  1:3003333/150‑1 (MQ=255)

                                                                                                                        ttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATg                          <  1:1109886/150‑1 (MQ=255)

                                                                                                                        ttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATg                          <  2:668316/150‑1 (MQ=255)

                                                                                                                           aCTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTaa                       >  1:189516/1‑150 (MQ=255)

                                                                                                                             ttGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAtt                     <  2:2945059/150‑1 (MQ=255)

                                                                                                                             ttGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAtt                     <  1:1839725/150‑1 (MQ=255)

                                                                                                                             ttGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAtt                     <  2:2945433/150‑1 (MQ=255)

                                                                                                                              tGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTa                    >  2:1804711/1‑150 (MQ=255)

                                                                                                                              tGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTa                    >  2:3006834/1‑150 (MQ=255)

                                                                                                                               gggAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTAc                   >  2:2310680/1‑150 (MQ=255)

                                                                                                                               gggAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTAc                   >  1:1172847/1‑150 (MQ=255)

                                                                                                                                ggAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACg                  <  2:1202618/150‑1 (MQ=255)

                                                                                                                                ggAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACg                  <  1:1367478/150‑1 (MQ=255)

                                                                                                                                 gagaAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGa                 >  2:3784823/1‑150 (MQ=255)

                                                                                                                                   gaAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGaaa               >  2:1033176/1‑150 (MQ=255)

                                                                                                                                       gggggTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGAc           <  1:1708719/150‑1 (MQ=255)

                                                                                                                                             gATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGctg     >  2:3193886/1‑148 (MQ=255)

                                                                                                                                               tCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGt   >  2:775643/1‑150 (MQ=255)

                                                                                                                                                cGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGtt  >  2:2525523/1‑150 (MQ=255)

GAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA‑TTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT  >  CP009273/647275‑647568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`