Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 61,967 A→C L433R (CTG→CGG)  rapA ← RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927361,9670AC99.5% 769.0 / NA 195L433R (CTG→CGG) rapARNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (105/89);  total (105/90)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

GCGTTATCACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGC  >  CP009273/61820‑62113
                                                                                                                                                   |                                                                                                                                                  
gCGTTATCACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGc                                                                                                                                                  >  2:3262013/1‑150 (MQ=255)
 cGTTATCACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCt                                                                                                                                                 >  2:191792/1‑150 (MQ=255)
 cGTTATCACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCt                                                                                                                                                 >  1:2293702/1‑150 (MQ=255)
    tATCACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTcgc                                                                                                                                              >  1:1012348/1‑150 (MQ=255)
     aTCACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTcgcg                                                                                                                                             >  2:806977/1‑150 (MQ=255)
     aTCACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTcgcg                                                                                                                                             >  1:994366/1‑150 (MQ=255)
       cACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGtt                                                                                                                                           >  2:1197272/1‑150 (MQ=255)
        aCCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGttt                                                                                                                                          >  2:1232244/1‑150 (MQ=255)
        aCCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGttt                                                                                                                                          >  1:1881294/1‑150 (MQ=255)
         ccTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTc                                                                                                                                         <  1:536271/150‑1 (MQ=255)
         ccTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTc                                                                                                                                         <  1:3262013/150‑1 (MQ=255)
          cTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCg                                                                                                                                        <  2:3673027/150‑1 (MQ=255)
          cTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCg                                                                                                                                        <  2:3558863/150‑1 (MQ=255)
          cTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCg                                                                                                                                        >  1:2360833/1‑150 (MQ=255)
           ttCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCgg                                                                                                                                       >  2:1540632/1‑150 (MQ=255)
              aaaTTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGaa                                                                                                                                    <  2:1850764/150‑1 (MQ=255)
               aaTTCCTGATACATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAAt                                                                                                                                   <  2:3406757/150‑1 (MQ=255)
               aaTTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAAt                                                                                                                                   <  2:547311/150‑1 (MQ=255)
                 ttCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATcc                                                                                                                                 <  1:3333537/150‑1 (MQ=255)
                  tCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCt                                                                                                                                <  2:2079148/150‑1 (MQ=255)
                  tCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCt                                                                                                                                >  2:2243270/1‑150 (MQ=255)
                     tGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTc                                                                                                                             >  1:2856578/1‑150 (MQ=255)
                       aTAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTcac                                                                                                                           >  1:2210643/1‑150 (MQ=255)
                        tAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTcaca                                                                                                                          <  2:2298414/150‑1 (MQ=255)
                          aaTACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACAcc                                                                                                                        >  2:772833/1‑150 (MQ=255)
                           aTACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCg                                                                                                                       <  1:1770350/150‑1 (MQ=255)
                           aTACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCg                                                                                                                       <  1:2971374/150‑1 (MQ=255)
                               gCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTAc                                                                                                                   >  1:3141676/1‑150 (MQ=255)
                                  ccGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTAcgcg                                                                                                                <  2:3172352/150‑1 (MQ=255)
                                       tAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTa                                                                                                           >  2:2801377/1‑150 (MQ=255)
                                       tAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTa                                                                                                           >  2:3469340/1‑150 (MQ=255)
                                        agagCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTAc                                                                                                          >  1:1695390/1‑150 (MQ=255)
                                        agagCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTAc                                                                                                          >  2:2970947/1‑150 (MQ=255)
                                         gagCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACg                                                                                                         >  2:2255650/1‑150 (MQ=255)
                                          agCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACgg                                                                                                        >  1:3709780/1‑150 (MQ=255)
                                           gCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGa                                                                                                       <  1:3073444/150‑1 (MQ=255)
                                            cATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGaa                                                                                                      >  1:3716817/1‑150 (MQ=255)
                                            cATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGaa                                                                                                      <  2:3055936/150‑1 (MQ=255)
                                             atatCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAAc                                                                                                     >  1:2373085/1‑150 (MQ=255)
                                               atCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAg                                                                                                   <  2:3162093/150‑1 (MQ=255)
                                               atCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAg                                                                                                   <  1:3227232/150‑1 (MQ=255)
                                                tCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATGGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGc                                                                                                  >  1:2068605/1‑150 (MQ=255)
                                                  gcgAGCACGATCTTCCGCACTTTTCCGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCAc                                                                                                <  2:2123308/150‑1 (MQ=255)
                                                   cgAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCAcg                                                                                               <  1:1107765/150‑1 (MQ=255)
                                                       cACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGc                                                                                           >  2:3038179/1‑150 (MQ=255)
                                                       cACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGc                                                                                           >  2:2882949/1‑150 (MQ=255)
                                                       cACGATCTTCAGCACTTTTACGTTCTCCCATAATGCCGGAGACTTTAATAGCCGTTTGATACTGCGTCGGTAGCGGCGGCTTTATGGTGTGCAGCTCGCGTTTCGTGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGc                                                                                           <  1:2285940/150‑1 (MQ=255)
                                                         cGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTg                                                                                         <  2:1351473/150‑1 (MQ=255)
                                                           aTCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGt                                                                                       >  1:1205957/1‑150 (MQ=255)
                                                           aTCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGt                                                                                       >  2:1406214/1‑150 (MQ=255)
                                                             cgtCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGc                                                                                     <  1:2945527/148‑1 (MQ=255)
                                                             cTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGc                                                                                     <  1:3363784/150‑1 (MQ=255)
                                                             cTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGc                                                                                     >  1:3751412/1‑150 (MQ=255)
                                                              ttCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGcc                                                                                    >  2:2581442/1‑150 (MQ=255)
                                                               tCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCg                                                                                   <  2:1114723/150‑1 (MQ=255)
                                                                 cGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTg                                                                                 <  1:2154512/150‑1 (MQ=255)
                                                                  gCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTgg                                                                                <  1:3401220/150‑1 (MQ=255)
                                                                   cACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGc                                                                               >  1:1515862/1‑150 (MQ=255)
                                                                      ttttACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGAt                                                                            >  1:613238/1‑150 (MQ=255)
                                                                      ttttACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGAt                                                                            <  1:2489748/150‑1 (MQ=255)
                                                                      ttttACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGAt                                                                            <  1:3262813/150‑1 (MQ=255)
                                                                       tttACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATc                                                                           >  2:2141229/1‑150 (MQ=255)
                                                                         tACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCa                                                                         >  1:1629825/1‑150 (MQ=255)
                                                                            gTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTATCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCa                                                                      >  1:847340/1‑150 (MQ=255)
                                                                             tGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAg                                                                     >  2:405364/1‑150 (MQ=255)
                                                                               cgcCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCa                                                                   >  1:2914769/1‑150 (MQ=255)
                                                                                  ccATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCg                                                                <  2:3795234/150‑1 (MQ=255)
                                                                                     tAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGaaa                                                             <  2:1695390/150‑1 (MQ=255)
                                                                                     tAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGaaa                                                             >  1:717030/1‑150 (MQ=255)
                                                                                     tAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGaaa                                                             >  2:616316/1‑150 (MQ=255)
                                                                                        tGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCa                                                          <  2:1438024/150‑1 (MQ=255)
                                                                                        tGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCa                                                          <  1:1319286/150‑1 (MQ=255)
                                                                                        tGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCa                                                          <  1:1773313/150‑1 (MQ=255)
                                                                                        tGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCa                                                          <  1:2110607/150‑1 (MQ=255)
                                                                                           cGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCt                                                       <  1:3038179/150‑1 (MQ=255)
                                                                                            ggAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTc                                                      >  1:262884/1‑150 (MQ=255)
                                                                                                 cTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGAGCCATCAGCATCGAAACCAGCTCCTGAc                                                 >  1:1509922/1‑150 (MQ=255)
                                                                                                  tttAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCGGCATCGAAACCAGCTCCTGACg                                                <  2:2289138/150‑1 (MQ=255)
                                                                                                  tttAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACg                                                >  1:2496674/1‑150 (MQ=255)
                                                                                                     aaTAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGgc                                             >  2:1007452/1‑150 (MQ=255)
                                                                                                      aTAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGgcg                                            <  1:3545529/150‑1 (MQ=255)
                                                                                                        aGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGct                                          >  2:764067/1‑150 (MQ=255)
                                                                                                          ccGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGGTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGctct                                        <  2:3085462/150‑1 (MQ=255)
                                                                                                            gTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTgg                                      >  2:1683890/1‑150 (MQ=255)
                                                                                                                gATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCAt                                  >  1:1942094/1‑150 (MQ=255)
                                                                                                                gATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCAt                                  <  1:1936764/150‑1 (MQ=255)
                                                                                                                 aTACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATc                                 >  2:2637440/1‑150 (MQ=255)
                                                                                                                    cTGCGTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTtc                              <  2:2914769/150‑1 (MQ=255)
                                                                                                                        gTCGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTtcgctg                          >  1:2233869/1‑150 (MQ=255)
                                                                                                                          cGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTtcgctgtc                        >  2:3783516/1‑150 (MQ=255)
                                                                                                                          cGGTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTtcgctgtc                        <  2:1537379/150‑1 (MQ=255)
                                                                                                                           ggTAGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTtcgctgtcg                       <  2:1790121/150‑1 (MQ=255)
                                                                                                                              aGCGGCAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTtcgctgtcgctg                    >  2:1061326/1‑150 (MQ=255)
                                                                                                                                   cAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTtgc               >  2:2509944/1‑150 (MQ=255)
                                                                                                                                   cAGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTtgc               >  2:2436060/1‑150 (MQ=255)
                                                                                                                                    aGCTTAATGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTtgct              <  2:3743022/150‑1 (MQ=255)
                                                                                                                                           tGGTGTGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCa       <  1:2143350/150‑1 (MQ=255)
                                                                                                                                             gtgtGCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAAc     >  1:663172/1‑150 (MQ=255)
                                                                                                                                              tgtgCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAANa    <  1:453556/150‑1 (MQ=255)
                                                                                                                                              tgtgCCGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACa    <  2:3340779/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTTATCACCTTCAAATTCCTGATAAATACGCTCCGGGTAGAGCATATCGCGAGCACGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGC  >  CP009273/61820‑62113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑