Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 753,549 G→T Q135H (CAG→CAT)  sdhB → succinate dehydrogenase, FeS subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273753,5490GT100.0% 600.0 / NA 183Q135H (CAG→CAT) sdhBsuccinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (93/90);  total (93/90)

Only 100 of 188 total aligned reads displayed.

CAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGA  >  CP009273/753402‑753695
                                                                                                                                                   |                                                                                                                                                  
cAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACatat                                                                                                                                                  <  1:2223067/150‑1 (MQ=255)
cAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACatat                                                                                                                                                  <  1:313207/150‑1 (MQ=255)
cAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACatat                                                                                                                                                  >  1:3504349/1‑150 (MQ=255)
cAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACatat                                                                                                                                                  >  2:2538511/1‑150 (MQ=255)
 aagaagATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATg                                                                                                                                                 >  2:3757095/1‑150 (MQ=255)
    aagaTTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCa                                                                                                                                              <  1:3687240/150‑1 (MQ=255)
        ttGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGagc                                                                                                                                          <  1:3652906/150‑1 (MQ=255)
          gtgATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGagcag                                                                                                                                        <  2:1175921/150‑1 (MQ=255)
          gtgATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGagcag                                                                                                                                        >  1:421074/1‑150 (MQ=255)
            gATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAgcg                                                                                                                                      >  2:1430814/1‑150 (MQ=255)
                 gCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGaaa                                                                                                                                 <  1:855603/150‑1 (MQ=255)
                     gCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACt                                                                                                                             >  1:1833261/1‑150 (MQ=255)
                     gCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACt                                                                                                                             <  1:2081789/150‑1 (MQ=255)
                          cAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACg                                                                                                                        >  1:1080536/1‑150 (MQ=255)
                          cAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAAATCGACg                                                                                                                        >  2:802779/1‑150 (MQ=255)
                            ggTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCATCGCGAAAAACTCGACggg                                                                                                                      >  2:2469357/1‑150 (MQ=255)
                            ggTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACggg                                                                                                                      >  1:1134915/1‑150 (MQ=255)
                            ggTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACggg                                                                                                                      >  1:3035511/1‑150 (MQ=255)
                              tttACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCt                                                                                                                    <  2:3403597/150‑1 (MQ=255)
                               ttACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTg                                                                                                                   <  1:2978234/150‑1 (MQ=255)
                                tACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGt                                                                                                                  <  1:2844069/150‑1 (MQ=255)
                                  ccGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTAt                                                                                                                <  2:96/150‑1 (MQ=255)
                                   cGGTGATCCGCGATTGGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATg                                                                                                               <  2:352755/150‑1 (MQ=255)
                                    ggTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGa                                                                                                              >  2:3724024/1‑150 (MQ=255)
                                      tGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAAt                                                                                                            <  2:3153633/150‑1 (MQ=255)
                                      tGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAAt                                                                                                            <  1:3030347/150‑1 (MQ=255)
                                          ccGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTAt                                                                                                        <  2:1212789/150‑1 (MQ=255)
                                            gcgATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATtc                                                                                                      <  1:1321077/150‑1 (MQ=255)
                                             cgATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATtct                                                                                                     <  1:848575/150‑1 (MQ=255)
                                               aTTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTctct                                                                                                   >  1:453297/1‑150 (MQ=255)
                                                 ttGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTgc                                                                                                 <  2:2399272/150‑1 (MQ=255)
                                                 ttGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTgc                                                                                                 >  1:3263626/1‑150 (MQ=255)
                                                          gACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCAtgttgt                                                                                        >  1:2782724/1‑150 (MQ=255)
                                                          gACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCAtgttgt                                                                                        >  1:445890/1‑150 (MQ=255)
                                                           aCATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATgttgtt                                                                                       <  1:2343162/150‑1 (MQ=255)
                                                            cATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTc                                                                                      <  2:1806588/150‑1 (MQ=255)
                                                                ggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAAcc                                                                                  <  2:1700049/150‑1 (MQ=255)
                                                                  aCAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACctc                                                                                >  1:2640389/1‑150 (MQ=255)
                                                                   cAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACctct                                                                               <  2:2800742/150‑1 (MQ=255)
                                                                    aaTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCtt                                                                              <  1:2459259/150‑1 (MQ=255)
                                                                    aaTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCtt                                                                              >  1:1472379/1‑150 (MQ=255)
                                                                    aaTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCtt                                                                              <  1:726024/150‑1 (MQ=255)
                                                                    aaTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCtt                                                                              >  1:2945722/1‑150 (MQ=255)
                                                                     atccTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTg                                                                             <  1:1524406/147‑1 (MQ=255)
                                                                       tCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTTTc                                                                           >  1:2579786/1‑150 (MQ=255)
                                                                       tCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTAAGAATGTAATCTCTGCGCATGTTGTTCAACCTCTTGTc                                                                           >  1:1176101/1‑150 (MQ=255)
                                                                         tATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCg                                                                         <  2:3691369/150‑1 (MQ=255)
                                                                             cgcAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCtt                                                                     >  1:3646973/1‑150 (MQ=255)
                                                                             cgcAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCtt                                                                     >  2:743826/1‑150 (MQ=255)
                                                                             cgcAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCtt                                                                     <  2:421074/150‑1 (MQ=255)
                                                                                 atatGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtg                                                                 <  2:548100/150‑1 (MQ=255)
                                                                                 atatGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtg                                                                 >  2:1051666/1‑150 (MQ=255)
                                                                                   atGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtggt                                                               <  2:1155238/150‑1 (MQ=255)
                                                                                      agaAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGa                                                            >  2:1628838/1‑150 (MQ=255)
                                                                                       gaAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGaa                                                           >  2:1974739/1‑150 (MQ=255)
                                                                                       gaAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGaa                                                           <  1:1010638/150‑1 (MQ=255)
                                                                                       gaAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGaa                                                           <  1:1010814/150‑1 (MQ=255)
                                                                                        aaaaTTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAAt                                                          >  2:1724429/1‑150 (MQ=255)
                                                                                          aaTTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATcc                                                        <  2:2826258/150‑1 (MQ=255)
                                                                                             tAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGa                                                     >  2:3555948/1‑150 (MQ=255)
                                                                                              aaGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGATCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGAt                                                    <  1:3757095/150‑1 (MQ=255)
                                                                                              aaGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGAt                                                    <  1:3234411/150‑1 (MQ=255)
                                                                                               aGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATa                                                   >  2:2106927/1‑150 (MQ=255)
                                                                                                    tACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGttt                                              <  1:178945/150‑1 (MQ=255)
                                                                                                     aCCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTa                                             >  2:2693488/1‑150 (MQ=255)
                                                                                                     aCCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTa                                             <  2:3540166/150‑1 (MQ=255)
                                                                                                        tGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCg                                          >  1:402546/1‑150 (MQ=255)
                                                                                                         gTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCgg                                         <  2:2716781/150‑1 (MQ=255)
                                                                                                           tGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGcc                                       >  1:2388628/1‑150 (MQ=255)
                                                                                                             aataatGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCg                                     <  1:1430814/150‑1 (MQ=255)
                                                                                                              ataatGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCgg                                    >  2:886364/1‑150 (MQ=255)
                                                                                                               taatGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGc                                   >  2:1367556/1‑150 (MQ=255)
                                                                                                               taatGGACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGc                                   >  2:3275246/1‑150 (MQ=255)
                                                                                                                    gACAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCt                              >  1:740332/1‑150 (MQ=255)
                                                                                                                     aCAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCtt                             <  1:757968/150‑1 (MQ=255)
                                                                                                                     aCAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCtt                             <  1:611092/150‑1 (MQ=255)
                                                                                                                      cAAAATCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTg                            <  2:3504349/150‑1 (MQ=255)
                                                                                                                        aaaTCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGtt                          >  2:1475907/1‑150 (MQ=255)
                                                                                                                        aaaTCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGtt                          >  2:348156/1‑150 (MQ=255)
                                                                                                                          aTCCGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGAGAAGTTTATCGGCCCGGCAGGCTTGTTAg                        >  2:404540/1‑150 (MQ=255)
                                                                                                                            ccGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCg                      <  1:1951441/150‑1 (MQ=255)
                                                                                                                            ccGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCg                      >  2:606085/1‑150 (MQ=255)
                                                                                                                            ccGCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCg                      <  1:1951308/150‑1 (MQ=255)
                                                                                                                              gCCAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGc                    >  2:267122/1‑150 (MQ=255)
                                                                                                                               ccAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCa                   <  2:3734048/150‑1 (MQ=255)
                                                                                                                               ccAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCa                   <  2:1833261/150‑1 (MQ=255)
                                                                                                                                cAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCat                  <  1:1216778/150‑1 (MQ=255)
                                                                                                                                cAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCat                  >  2:214358/1‑150 (MQ=255)
                                                                                                                                cAGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCat                  >  2:417280/1‑150 (MQ=255)
                                                                                                                                 aGCTCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCata                 <  1:2538511/150‑1 (MQ=255)
                                                                                                                                    tCGCGAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCg              >  2:2203302/1‑150 (MQ=255)
                                                                                                                                      gcgAGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGtt            <  1:2195231/150‑1 (MQ=255)
                                                                                                                                         aGCATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTcc         >  1:2937792/1‑150 (MQ=255)
                                                                                                                                           cATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtg       >  1:2202436/1‑150 (MQ=255)
                                                                                                                                           cATTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtg       >  1:570491/1‑150 (MQ=255)
                                                                                                                                            aTTTACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtga      <  2:13281/150‑1 (MQ=255)
                                                                                                                                             tttACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgat     <  1:3333251/150‑1 (MQ=255)
                                                                                                                                               tACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattg   <  2:3075085/150‑1 (MQ=255)
                                                                                                                                               tACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattg   >  2:2567212/1‑150 (MQ=255)
                                                                                                                                               tACATATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattg   <  1:1051666/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGA  >  CP009273/753402‑753695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑