Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 78,007 C→A G147C (GGT→TGT)  leuB ← 3‑isopropylmalate dehydrogenase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927378,0070CA100.0% 734.0 / NA 219G147C (GGT→TGT) leuB3‑isopropylmalate dehydrogenase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (103/116);  total (103/116)

Only 100 of 219 total aligned reads displayed.

CTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAGTGCTTACGCA  >  CP009273/77860‑78153
                                                                                                                                                   |                                                                                                                                                  
     tcgatcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAg                                                                                                                                             >  1:3774423/1‑150 (MQ=255)
       gatcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGAt                                                                                                                                           <  1:1283073/150‑1 (MQ=255)
         tcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATgc                                                                                                                                         <  2:729024/150‑1 (MQ=255)
         tcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATgc                                                                                                                                         >  2:661251/1‑150 (MQ=255)
         tcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATgc                                                                                                                                         <  2:2447395/150‑1 (MQ=255)
         tcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATgc                                                                                                                                         >  1:2086559/1‑150 (MQ=255)
          cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATgcc                                                                                                                                        >  1:2101726/1‑150 (MQ=255)
             cGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATgccgcc                                                                                                                                     <  2:2591717/150‑1 (MQ=255)
              gTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGccgccg                                                                                                                                    >  1:2658696/1‑150 (MQ=255)
              gTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGccgccg                                                                                                                                    >  1:41456/1‑150 (MQ=255)
                  cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCa                                                                                                                                <  2:2260005/150‑1 (MQ=255)
                   tttGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAg                                                                                                                               <  1:1702285/150‑1 (MQ=255)
                   tttGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAg                                                                                                                               <  2:3763500/150‑1 (MQ=255)
                     tgtgGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGtt                                                                                                                             <  2:1787044/150‑1 (MQ=255)
                     tgtgGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGtt                                                                                                                             <  1:676462/150‑1 (MQ=255)
                     tgtgGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGtt                                                                                                                             <  1:513543/150‑1 (MQ=255)
                     tgtgGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGtt                                                                                                                             <  2:2897725/150‑1 (MQ=255)
                      gtgGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTc                                                                                                                            <  1:1315657/150‑1 (MQ=255)
                               cTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGACATAGATGCCGCCGGTCAGTTCGCGcacaca                                                                                                                   >  1:1969340/1‑150 (MQ=255)
                                   cGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAgg                                                                                                               >  2:854326/1‑150 (MQ=255)
                                    gAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGa                                                                                                              >  1:3546283/1‑150 (MQ=255)
                                     aGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGAt                                                                                                             >  2:1115556/1‑150 (MQ=255)
                                        aGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTc                                                                                                          >  2:1698694/1‑150 (MQ=255)
                                        aGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTc                                                                                                          >  1:1042025/1‑150 (MQ=255)
                                        aGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCCTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTc                                                                                                          <  2:2387419/150‑1 (MQ=255)
                                         gATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCg                                                                                                         >  1:2152348/1‑150 (MQ=255)
                                         gATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCg                                                                                                         >  2:2350812/1‑150 (MQ=255)
                                         gATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCg                                                                                                         <  2:159690/150‑1 (MQ=255)
                                            tCAAACGCGATGCGGGCGATACGTTCGATCTCTATTCGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTTCCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAg                                                                                                      <  2:1339851/150‑1 (MQ=255)
                                              aaaCGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGcc                                                                                                    <  2:2787941/150‑1 (MQ=255)
                                              aaaCGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGcc                                                                                                    >  2:2201809/1‑150 (MQ=255)
                                                 cgcgATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGtt                                                                                                 <  1:945375/150‑1 (MQ=255)
                                                  gcgATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGttt                                                                                                <  2:2043386/150‑1 (MQ=255)
                                                   cgATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTg                                                                                               <  1:2106872/150‑1 (MQ=255)
                                                   cgATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTg                                                                                               <  1:2147120/150‑1 (MQ=255)
                                                     aTGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCg                                                                                             >  1:1682409/1‑150 (MQ=255)
                                                     aTGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCg                                                                                             <  1:2285794/150‑1 (MQ=255)
                                                        cGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCa                                                                                          >  2:3798068/1‑150 (MQ=255)
                                                        cGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCa                                                                                          <  1:1783436/150‑1 (MQ=255)
                                                         gggCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCaa                                                                                         >  1:3126126/1‑150 (MQ=255)
                                                           gCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATg                                                                                       <  2:325370/150‑1 (MQ=255)
                                                              aTACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCt                                                                                    <  2:459710/150‑1 (MQ=255)
                                                                  gTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCAc                                                                                <  1:1559800/150‑1 (MQ=255)
                                                                    tCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGc                                                                              >  2:962484/1‑150 (MQ=255)
                                                                      gATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAg                                                                            >  1:2778626/1‑150 (MQ=255)
                                                                        tctcAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCg                                                                          <  2:301055/150‑1 (MQ=255)
                                                                        tctcAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCg                                                                          <  1:2915224/150‑1 (MQ=255)
                                                                         ctcAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCgg                                                                         >  2:520638/1‑150 (MQ=255)
                                                                         ctcAAAACGGTGATACACCTCGGTATCAAAGGCATTTTCATATAGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCgg                                                                         >  2:3079122/1‑150 (MQ=255)
                                                                          tcAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGa                                                                        <  1:2358640/150‑1 (MQ=255)
                                                                            aaaaCGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCACTGTCTGCACGCAGCGGACa                                                                      >  1:112600/1‑150 (MQ=255)
                                                                            aaaaCGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACa                                                                      >  1:2258851/1‑150 (MQ=255)
                                                                             aaaCGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAg                                                                     >  1:1170900/1‑150 (MQ=255)
                                                                                cGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAAt                                                                  <  1:2188909/150‑1 (MQ=255)
                                                                                 ggTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATg                                                                 <  1:2724914/150‑1 (MQ=255)
                                                                                    gATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCtt                                                              <  1:2567481/150‑1 (MQ=255)
                                                                                     aTACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTc                                                             <  2:3695330/150‑1 (MQ=255)
                                                                                       acacCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCa                                                           >  2:292146/1‑150 (MQ=255)
                                                                                        cacCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAg                                                          <  2:1319424/150‑1 (MQ=255)
                                                                                           cTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGccc                                                       >  2:3367606/1‑150 (MQ=255)
                                                                                              ggTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTg                                                    <  2:2147451/150‑1 (MQ=255)
                                                                                              ggTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTg                                                    >  1:2629225/1‑150 (MQ=255)
                                                                                              ggTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTg                                                    >  1:3637189/1‑150 (MQ=255)
                                                                                              ggTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTg                                                    >  1:2629249/1‑150 (MQ=255)
                                                                                                tATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGAt                                                  >  1:1415002/1‑150 (MQ=255)
                                                                                                 aTCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATa                                                 >  1:136036/1‑150 (MQ=255)
                                                                                                   cAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACa                                               >  2:1591698/1‑150 (MQ=255)
                                                                                                     aaGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGt                                             <  2:2091398/150‑1 (MQ=255)
                                                                                                      aGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGtt                                            <  2:3546283/150‑1 (MQ=255)
                                                                                                        gCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGtttt                                          <  2:2300038/150‑1 (MQ=255)
                                                                                                         cTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTg                                         >  2:780771/1‑150 (MQ=255)
                                                                                                           ttttCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGcc                                       <  1:2126560/150‑1 (MQ=255)
                                                                                                           ttttCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGcc                                       <  1:850811/150‑1 (MQ=255)
                                                                                                              tCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCggg                                    <  2:1032391/150‑1 (MQ=255)
                                                                                                                atatTGTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGgcg                                  >  2:3579117/1‑150 (MQ=255)
                                                                                                                     gTCCGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGt                             <  2:2207273/150‑1 (MQ=255)
                                                                                                                       ccGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTg                           >  2:434646/1‑150 (MQ=255)
                                                                                                                        cGCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGc                          >  2:2366611/1‑150 (MQ=255)
                                                                                                                         gcgaCCGTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCt                         <  2:1835940/147‑1 (MQ=255)
                                                                                                                         gCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCt                         >  2:2558360/1‑150 (MQ=255)
                                                                                                                         gCTACCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCt                         >  2:651712/1‑150 (MQ=255)
                                                                                                                            aCCTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaa                      >  2:1333125/1‑150 (MQ=255)
                                                                                                                             ccTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaat                     <  1:1698694/150‑1 (MQ=255)
                                                                                                                             ccTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaat                     >  1:1135793/1‑150 (MQ=255)
                                                                                                                             ccTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaat                     <  2:2784707/150‑1 (MQ=255)
                                                                                                                             ccTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaat                     >  1:3224082/1‑150 (MQ=255)
                                                                                                                             ccTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaat                     <  1:333129/150‑1 (MQ=255)
                                                                                                                             ccTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaat                     <  1:520638/150‑1 (MQ=255)
                                                                                                                             ccTTCGCGGCCTTTGGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaat                     <  1:3079122/150‑1 (MQ=255)
                                                                                                                              cTTCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaata                    >  2:2615979/1‑150 (MQ=255)
                                                                                                                               ttCGCGGCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGaataa                   <  1:1399105/150‑1 (MQ=255)
                                                                                                                                    ggCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGa              >  2:2492133/1‑150 (MQ=255)
                                                                                                                                     gCCTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGaa             <  1:2416889/150‑1 (MQ=255)
                                                                                                                                      ccTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAg            <  2:3147488/150‑1 (MQ=255)
                                                                                                                                      ccTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAg            <  1:600046/150‑1 (MQ=255)
                                                                                                                                       cTTTTGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAGt           >  2:622051/1‑150 (MQ=255)
                                                                                                                                          ttGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAGTGCt        <  2:201641/150‑1 (MQ=255)
                                                                                                                                          ttGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAGTGCt        <  1:748042/150‑1 (MQ=255)
                                                                                                                                           tGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAGTGCtt       <  2:2778626/150‑1 (MQ=255)
                                                                                                                                           tGGCTGACAGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAGTGCtt       <  2:2658696/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGAATAATTTGAAGTGCTTACGCA  >  CP009273/77860‑78153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑