BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	837,716	G→T	intergenic (‑204/‑72)	mcbA ← / → rlmF	colanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	837,716	0	G	T	100.0%	784.4 / NA	233	intergenic (‑204/‑72)	mcbA/rlmF	colanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (139/94); total (139/94)

Only 100 of 235 total aligned reads displayed.

GATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAG  >  CP009273/837569‑837862

gATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTAt                                                                                                                                                  <  2:1600149/150‑1 (MQ=255)

 aTGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATtata                                                                                                                                                 <  2:1557868/150‑1 (MQ=255)

    cAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAAc                                                                                                                                              <  2:2513129/150‑1 (MQ=255)

     aaaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAGTGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACg                                                                                                                                             >  1:875293/1‑150 (MQ=255)

     aaaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACg                                                                                                                                             >  1:1295788/1‑150 (MQ=255)

        tGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAg                                                                                                                                          <  2:2916070/150‑1 (MQ=255)

            gCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAg                                                                                                                                      >  1:3033053/1‑150 (MQ=255)

             cAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGa                                                                                                                                     >  1:2235960/1‑150 (MQ=255)

              aaaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGat                                                                                                                                    >  1:602520/1‑150 (MQ=255)

                aaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGatat                                                                                                                                  <  1:2688582/150‑1 (MQ=255)

                 aaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATAtt                                                                                                                                 <  1:1239803/150‑1 (MQ=255)

                  aaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATAttt                                                                                                                                >  2:829737/1‑150 (MQ=255)

                  aaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATAttt                                                                                                                                >  2:125982/1‑150 (MQ=255)

                   aTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTa                                                                                                                               <  2:2410075/150‑1 (MQ=255)

                    ttGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTAc                                                                                                                              <  1:3796021/150‑1 (MQ=255)

                     tGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTAcc                                                                                                                             >  1:3378671/1‑150 (MQ=255)

                      gACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTAcac                                                                                                                            >  2:204842/1‑148 (MQ=255)

                           agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTcg                                                                                                                       >  2:2746033/1‑150 (MQ=255)

                           agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTcg                                                                                                                       <  2:3252092/150‑1 (MQ=255)

                           agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTcg                                                                                                                       >  2:340845/1‑150 (MQ=255)

                           agaTGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTcg                                                                                                                       <  2:3485698/150‑1 (MQ=255)

                               gtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGtt                                                                                                                   >  1:53778/1‑150 (MQ=255)

                                       tttttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGt                                                                                                           >  2:512272/1‑150 (MQ=255)

                                         tttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTAt                                                                                                         <  1:3532461/150‑1 (MQ=255)

                                         tttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTAt                                                                                                         <  2:1930045/150‑1 (MQ=255)

                                           tGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCt                                                                                                       <  1:1059363/150‑1 (MQ=255)

                                            gAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCtt                                                                                                      >  2:1905142/1‑150 (MQ=255)

                                            gAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCtt                                                                                                      <  1:1912115/150‑1 (MQ=255)

                                             aaCGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCCGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTCAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTa                                                                                                     >  2:1283342/1‑150 (MQ=255)

                                             aaCGAAATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTa                                                                                                     >  2:1651959/1‑150 (MQ=255)

                                                 atatatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgcc                                                                                                 >  2:2187682/1‑150 (MQ=255)

                                                    tatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgct                                                                                              <  1:2088159/150‑1 (MQ=255)

                                                      tttttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgctgc                                                                                            <  1:3245863/150‑1 (MQ=255)

                                                       ttttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgctgct                                                                                           <  2:2971443/150‑1 (MQ=255)

                                                         ttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATACCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTa                                                                                         <  2:2121815/150‑1 (MQ=255)

                                                          tACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTaa                                                                                        <  2:2024002/150‑1 (MQ=255)

                                                           aCAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTaaa                                                                                       >  2:894383/1‑150 (MQ=255)

                                                             aaaaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAgg                                                                                     >  1:838319/1‑150 (MQ=255)

                                                               aaTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgag                                                                                   >  1:3353899/1‑150 (MQ=255)

                                                                   aGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                               <  2:1743936/150‑1 (MQ=255)

                                                                   aGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                               <  1:2564027/150‑1 (MQ=255)

                                                                    gACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGc                                                                              >  1:280718/1‑150 (MQ=255)

                                                                     aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCt                                                                             >  2:3519905/1‑150 (MQ=255)

                                                                     aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCt                                                                             >  2:3445353/1‑150 (MQ=255)

                                                                      cAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTa                                                                            >  2:1965816/1‑150 (MQ=255)

                                                                        taTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATg                                                                          >  2:542629/2‑150 (MQ=255)

                                                                        aaTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATg                                                                          <  1:2666703/150‑1 (MQ=255)

                                                                        aaTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATg                                                                          >  1:2425860/1‑150 (MQ=255)

                                                                        aaTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATg                                                                          >  1:3170639/1‑150 (MQ=255)

                                                                         aTCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGt                                                                         >  1:394410/1‑150 (MQ=255)

                                                                          tCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTc                                                                        <  1:959199/150‑1 (MQ=255)

                                                                           cAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTcc                                                                       >  2:3629040/1‑150 (MQ=255)

                                                                           cAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTcc                                                                       >  2:3629309/1‑150 (MQ=255)

                                                                                aCATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCa                                                                  <  1:2843554/150‑1 (MQ=255)

                                                                                 cATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAg                                                                 >  1:1336771/1‑150 (MQ=255)

                                                                                  aTGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGa                                                                >  2:2984946/1‑150 (MQ=255)

                                                                                   tGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGaa                                                               <  1:1457546/150‑1 (MQ=255)

                                                                                     cATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAAc                                                             >  1:942219/1‑150 (MQ=255)

                                                                                        aaTGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCgg                                                          >  1:3032559/1‑150 (MQ=255)

                                                                                         aTGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGACTTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTCTCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGACACCggg                                                         >  1:1130176/1‑150 (MQ=255)

                                                                                            cTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGtt                                                      <  1:216252/150‑1 (MQ=255)

                                                                                            cTGATTTTTATGATGAATTGACATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGtt                                                      <  1:2024366/150‑1 (MQ=255)

                                                                                             tGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTg                                                     <  2:540584/150‑1 (MQ=255)

                                                                                             tGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTg                                                     <  2:3178989/150‑1 (MQ=255)

                                                                                             tGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTg                                                     <  2:1295788/150‑1 (MQ=255)

                                                                                               aTTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCa                                                   <  1:754035/150‑1 (MQ=255)

                                                                                               aTTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCa                                                   >  1:2949560/1‑150 (MQ=255)

                                                                                                  tttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATcc                                                >  2:2149656/1‑150 (MQ=255)

                                                                                                      tgatgaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCgcgc                                            >  1:338673/1‑150 (MQ=255)

                                                                                                        atgaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCaa                                          >  1:647423/1‑150 (MQ=255)

                                                                                                           aaTTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCg                                       >  2:2583502/1‑150 (MQ=255)

                                                                                                           aaTTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCg                                       >  2:2660563/1‑150 (MQ=255)

                                                                                                            aTTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGt                                      >  1:3089254/1‑150 (MQ=255)

                                                                                                                  aTAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                <  2:1472911/150‑1 (MQ=255)

                                                                                                                  aTAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                >  2:702978/1‑150 (MQ=255)

                                                                                                                  aTAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                >  2:3198888/1‑150 (MQ=255)

                                                                                                                  aTAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                                >  2:1525354/1‑150 (MQ=255)

                                                                                                                   tAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcaca                               <  1:1225813/150‑1 (MQ=255)

                                                                                                                   tAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcaca                               <  1:1153849/150‑1 (MQ=255)

                                                                                                                    aaaCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAg                              <  2:1132053/150‑1 (MQ=255)

                                                                                                                      aCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGcc                            <  2:2850372/150‑1 (MQ=255)

                                                                                                                      aCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGcc                            <  2:602520/150‑1 (MQ=255)

                                                                                                                      aCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGcc                            <  1:2651592/150‑1 (MQ=255)

                                                                                                                        cATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGc                          >  2:387441/1‑150 (MQ=255)

                                                                                                                         aTAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCt                         <  1:2458288/150‑1 (MQ=255)

                                                                                                                         aTAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCt                         <  1:2868800/150‑1 (MQ=255)

                                                                                                                           tgCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTAc                       <  1:2893965/149‑1 (MQ=255)

                                                                                                                           aGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTAc                       <  1:512272/150‑1 (MQ=255)

                                                                                                                           aGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTAc                       <  2:1238618/150‑1 (MQ=255)

                                                                                                                            gCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACg                      <  2:2932263/150‑1 (MQ=255)

                                                                                                                              cATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAt                    >  2:223882/1‑150 (MQ=255)

                                                                                                                                tGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtct                  <  1:1739/150‑1 (MQ=255)

                                                                                                                                     aGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCa             >  2:3352974/1‑150 (MQ=255)

                                                                                                                                     aGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCa             >  2:2997237/1‑150 (MQ=255)

                                                                                                                                      ggTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCAc            >  2:1091951/1‑150 (MQ=255)

                                                                                                                                      ggTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCAc            >  2:682448/1‑150 (MQ=255)

                                                                                                                                      ggTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCAc            >  2:2677793/1‑150 (MQ=255)

                                                                                                                                            ggggAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTg      >  2:3111262/1‑150 (MQ=255)

                                                                                                                                               gAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCa   >  2:1613673/1‑150 (MQ=255)

                                                                                                                                                aaTTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAg  >  1:1977279/1‑150 (MQ=255)

GATGCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAG  >  CP009273/837569‑837862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`