Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 868,500 A→T S22S (TCA→TCT)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273868,5000AT100.0% 687.3 / NA 208S22S (TCA→TCT) yliEputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (107/101);  total (107/101)

Only 100 of 218 total aligned reads displayed.

TAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC  >  CP009273/868353‑868646
                                                                                                                                                   |                                                                                                                                                  
 aaTTTGGTTAAAAAGCCGCATCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 <  1:2182915/150‑1 (MQ=255)
 aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 >  2:3275028/1‑150 (MQ=255)
 aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 <  2:1695650/150‑1 (MQ=255)
 aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 >  2:2204608/1‑150 (MQ=255)
  aTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTa                                                                                                                                                <  2:3300935/150‑1 (MQ=255)
  aTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTa                                                                                                                                                <  1:2575882/150‑1 (MQ=255)
   tttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAt                                                                                                                                               <  2:2017027/150‑1 (MQ=255)
   tttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAt                                                                                                                                               <  1:863906/150‑1 (MQ=255)
   tttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAt                                                                                                                                               >  2:2380342/1‑150 (MQ=255)
     tGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTg                                                                                                                                             <  1:2051930/150‑1 (MQ=255)
         tAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCt                                                                                                                                         >  1:2704776/1‑150 (MQ=255)
          aaaaaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCtt                                                                                                                                        <  2:3149000/150‑1 (MQ=255)
           aaaaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCttt                                                                                                                                       <  1:1947520/150‑1 (MQ=255)
            aaaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCtttt                                                                                                                                      >  1:1156967/1‑150 (MQ=255)
            aaaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCtttt                                                                                                                                      >  1:1638847/1‑150 (MQ=255)
              aGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCtttttt                                                                                                                                    >  2:3636799/1‑150 (MQ=255)
                     gcaAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTcatcat                                                                                                                             <  1:3288518/150‑1 (MQ=255)
                       aaGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTa                                                                                                                           >  2:678949/1‑150 (MQ=255)
                       aaGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTa                                                                                                                           <  2:3257167/150‑1 (MQ=255)
                       aaGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTa                                                                                                                           >  2:3489048/1‑150 (MQ=255)
                         gggACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAAc                                                                                                                         >  1:790467/1‑150 (MQ=255)
                           gACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTa                                                                                                                       <  2:1209358/150‑1 (MQ=255)
                              aaTTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCa                                                                                                                    <  1:2564819/150‑1 (MQ=255)
                              aaTTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCa                                                                                                                    <  1:1794701/150‑1 (MQ=255)
                                tttttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAAc                                                                                                                  <  1:3411735/150‑1 (MQ=255)
                                 ttttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACt                                                                                                                 <  1:2885930/150‑1 (MQ=255)
                                   ttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTgg                                                                                                               <  2:2835919/150‑1 (MQ=255)
                                   ttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTgg                                                                                                               <  1:497389/150‑1 (MQ=255)
                                   ttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAACGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTgg                                                                                                               <  2:1775372/150‑1 (MQ=255)
                                    tGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATACTTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGt                                                                                                              >  2:3598473/1‑150 (MQ=255)
                                       aGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATc                                                                                                           <  1:1064860/150‑1 (MQ=255)
                                            cacaGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAgc                                                                                                      <  2:1302393/150‑1 (MQ=255)
                                            cacaGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATAAGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAgc                                                                                                      <  1:3526261/150‑1 (MQ=255)
                                               aGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAgcgcg                                                                                                   >  1:3134450/1‑150 (MQ=255)
                                               aGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAgcgcg                                                                                                   <  2:608218/150‑1 (MQ=255)
                                                        aTAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAc                                                                                          >  2:1916043/1‑150 (MQ=255)
                                                        aTAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAc                                                                                          >  2:1390522/1‑150 (MQ=255)
                                                        aTAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAc                                                                                          <  1:145349/150‑1 (MQ=255)
                                                           gTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTg                                                                                       >  1:2811741/1‑150 (MQ=255)
                                                            ttATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGc                                                                                      <  2:3712405/150‑1 (MQ=255)
                                                             tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGcc                                                                                     >  2:2408434/1‑150 (MQ=255)
                                                             tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGcc                                                                                     <  1:3640803/150‑1 (MQ=255)
                                                                tttGTTAAATGTATTCACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGAt                                                                                  <  2:2734421/150‑1 (MQ=255)
                                                                 ttGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTGTCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATa                                                                                 <  2:1220983/150‑1 (MQ=255)
                                                                 ttGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATa                                                                                 >  1:2409069/1‑150 (MQ=255)
                                                                    ttAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGcc                                                                              >  2:1737406/1‑150 (MQ=255)
                                                                      aaaTGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGc                                                                            >  1:1324474/1‑150 (MQ=255)
                                                                       aaTGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCt                                                                           <  2:3081715/150‑1 (MQ=255)
                                                                       aaTGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCt                                                                           <  2:2742877/150‑1 (MQ=255)
                                                                         tGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCttt                                                                         >  1:3584574/1‑150 (MQ=255)
                                                                          gTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTg                                                                        >  2:782474/1‑150 (MQ=255)
                                                                           tATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGa                                                                       >  1:2796521/1‑150 (MQ=255)
                                                                            aTTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGaa                                                                      >  1:1623831/1‑150 (MQ=255)
                                                                               aaCATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACtt                                                                   >  1:1308939/1‑150 (MQ=255)
                                                                                  aTGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAtt                                                                <  2:1196640/150‑1 (MQ=255)
                                                                                    gCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCa                                                              <  2:2846713/150‑1 (MQ=255)
                                                                                         gTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaaaa                                                         <  1:719679/150‑1 (MQ=255)
                                                                                         gTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaaaa                                                         <  1:2624504/150‑1 (MQ=255)
                                                                                           ttATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAAc                                                       >  1:1410040/1‑150 (MQ=255)
                                                                                             ataCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGt                                                     >  1:157195/1‑150 (MQ=255)
                                                                                              taCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGtt                                                    <  1:213787/150‑1 (MQ=255)
                                                                                              taCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGtt                                                    <  1:3603737/150‑1 (MQ=255)
                                                                                                cGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTgg                                                  >  2:248795/1‑150 (MQ=255)
                                                                                                 gAAAAGATAAAGATAAGGCTGATATTTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGATTTGAACTTATTCAGAAAAACGTTGGc                                                 <  2:2042701/150‑1 (MQ=255)
                                                                                                 gAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGc                                                 >  1:2966035/1‑150 (MQ=255)
                                                                                                  aaaaGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCt                                                >  2:86460/1‑150 (MQ=255)
                                                                                                  aaaaGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCt                                                <  1:3489048/150‑1 (MQ=255)
                                                                                                    aagataaagataAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAt                                              >  1:988404/1‑150 (MQ=255)
                                                                                                    aagataaagataAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGAAAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAt                                              >  1:197755/1‑150 (MQ=255)
                                                                                                      gataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAttt                                            <  1:614483/150‑1 (MQ=255)
                                                                                                       ataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTc                                           >  1:712312/1‑150 (MQ=255)
                                                                                                       ataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTc                                           >  1:2347181/1‑150 (MQ=255)
                                                                                                       ataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTATTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTc                                           <  1:2240003/150‑1 (MQ=255)
                                                                                                        taaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCt                                          <  2:470721/150‑1 (MQ=255)
                                                                                                        taaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCt                                          <  1:3332658/150‑1 (MQ=255)
                                                                                                         aaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCtt                                         >  1:1821637/1‑150 (MQ=255)
                                                                                                            gataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTaa                                      >  1:3758261/1‑150 (MQ=255)
                                                                                                               aaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGa                                   >  1:3243255/1‑150 (MQ=255)
                                                                                                                aGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGat                                  <  1:678949/150‑1 (MQ=255)
                                                                                                                  gCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGatat                                <  1:2204589/150‑1 (MQ=255)
                                                                                                                  gCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGAGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGagat                                <  2:1573981/150‑1 (MQ=255)
                                                                                                                    tGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTg                              <  2:3260009/150‑1 (MQ=255)
                                                                                                                     gATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGa                             >  1:2484544/1‑150 (MQ=255)
                                                                                                                       tAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGTAc                           >  2:219385/1‑150 (MQ=255)
                                                                                                                          ttttatttttatTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGtt                        >  2:483292/1‑150 (MQ=255)
                                                                                                                           tttatttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTc                       <  1:735978/150‑1 (MQ=255)
                                                                                                                             tatttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCgg                     >  1:2898207/1‑150 (MQ=255)
                                                                                                                              atttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGc                    >  1:1509559/1‑150 (MQ=255)
                                                                                                                                 tttattGGCAGCTCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCt                 <  1:3598473/150‑1 (MQ=255)
                                                                                                                                 tttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCt                 >  2:297756/1‑150 (MQ=255)
                                                                                                                                  ttattGGCAGCACTGTCTTTTATTGGTATTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTg                <  2:3128004/150‑1 (MQ=255)
                                                                                                                                    attGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGAc              >  2:3373312/1‑150 (MQ=255)
                                                                                                                                    attGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGAc              >  2:605066/1‑150 (MQ=255)
                                                                                                                                    attGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGAc              >  1:2083784/1‑150 (MQ=255)
                                                                                                                                      tGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAt            >  1:3047003/1‑150 (MQ=255)
                                                                                                                                      tGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAt            >  2:2077160/1‑150 (MQ=255)
                                                                                                                                        gcagcaCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTa          <  2:1835741/150‑1 (MQ=255)
                                                                                                                                          agcaCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTaaa        <  2:2154118/150‑1 (MQ=255)
                                                                                                                                             aCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGa     >  1:3432697/1‑150 (MQ=255)
                                                                                                                                               tGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACt   >  1:534656/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC  >  CP009273/868353‑868646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑