Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 994,328 G→T G128V (GGA→GTA)  elfD → putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273994,3280GT100.0% 765.9 / NA 229G128V (GGA→GTA) elfDputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (130/99);  total (130/99)

Only 100 of 229 total aligned reads displayed.

TTATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA  >  CP009273/994181‑994472
                                                                                                                                                   |                                                                                                                                                
ttattaCACCACTGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTaa                                                                                                                                                >  2:3212298/1‑150 (MQ=255)
ttattaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTaa                                                                                                                                                >  1:3437067/1‑150 (MQ=255)
  attaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAt                                                                                                                                              >  1:17570/1‑150 (MQ=255)
  attaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAt                                                                                                                                              <  2:2616054/150‑1 (MQ=255)
  attaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAt                                                                                                                                              >  2:1153805/1‑150 (MQ=255)
   ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATa                                                                                                                                             >  1:1092382/1‑150 (MQ=255)
   ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATa                                                                                                                                             >  2:2229721/1‑150 (MQ=255)
   ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATa                                                                                                                                             <  1:1657852/150‑1 (MQ=255)
     acacCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAcc                                                                                                                                           <  2:1778838/150‑1 (MQ=255)
      caccacCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAccc                                                                                                                                          >  1:944713/1‑150 (MQ=255)
        ccaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTg                                                                                                                                        >  1:1235709/1‑150 (MQ=255)
           ccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAg                                                                                                                                     <  2:341349/150‑1 (MQ=255)
            cGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGa                                                                                                                                    >  1:614207/1‑150 (MQ=255)
             gCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGAt                                                                                                                                   >  2:1911978/1‑150 (MQ=255)
                aTTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCTCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACAAGCATACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAAACCCTGAAGATTGc                                                                                                                                >  1:1958312/1‑150 (MQ=255)
                      gCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcacaca                                                                                                                          >  1:2244040/1‑150 (MQ=255)
                         gAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAg                                                                                                                       >  2:732058/1‑150 (MQ=255)
                           aTCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCg                                                                                                                     <  1:445745/150‑1 (MQ=255)
                           aTCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCg                                                                                                                     >  1:2031329/1‑150 (MQ=255)
                            tCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGt                                                                                                                    >  1:1683150/1‑150 (MQ=255)
                            tCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGt                                                                                                                    >  1:3578997/1‑150 (MQ=255)
                            tCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGt                                                                                                                    >  1:3508616/1‑150 (MQ=255)
                             ccGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtc                                                                                                                   <  1:2506049/150‑1 (MQ=255)
                              cGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtca                                                                                                                  >  1:1649796/1‑150 (MQ=255)
                                gCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtcatc                                                                                                                >  2:1440993/1‑150 (MQ=255)
                                 cAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtcatca                                                                                                               >  2:3032145/1‑150 (MQ=255)
                                    aTGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAAc                                                                                                            <  2:3073446/150‑1 (MQ=255)
                                       agaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                         >  2:292175/1‑150 (MQ=255)
                                       agaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                         <  1:3622374/150‑1 (MQ=255)
                                        gaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACtttt                                                                                                        >  1:3052339/1‑150 (MQ=255)
                                        gaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACtttt                                                                                                        >  2:1055537/1‑150 (MQ=255)
                                          aTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCt                                                                                                      <  1:1386814/150‑1 (MQ=255)
                                          aTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCt                                                                                                      >  1:179746/1‑150 (MQ=255)
                                          aTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCt                                                                                                      <  2:3360824/150‑1 (MQ=255)
                                          aTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCt                                                                                                      <  1:2179807/150‑1 (MQ=255)
                                          aTCTGTGACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCt                                                                                                      <  1:2921908/150‑1 (MQ=255)
                                           tCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTg                                                                                                     >  2:3104211/1‑150 (MQ=255)
                                            cTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTgg                                                                                                    <  1:866564/150‑1 (MQ=255)
                                             tGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGc                                                                                                   >  1:1601134/1‑150 (MQ=255)
                                             tGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGc                                                                                                   <  2:430917/150‑1 (MQ=255)
                                                tACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGAc                                                                                                <  2:1935150/150‑1 (MQ=255)
                                                  cGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCa                                                                                              <  2:2423914/150‑1 (MQ=255)
                                                    cATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaa                                                                                            <  2:67433/150‑1 (MQ=255)
                                                          gTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGttt                                                                                      <  1:280131/150‑1 (MQ=255)
                                                          gTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGttt                                                                                      <  2:2788167/150‑1 (MQ=255)
                                                            aCATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAg                                                                                    <  2:2194772/150‑1 (MQ=255)
                                                            aCATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAg                                                                                    <  1:3212298/150‑1 (MQ=255)
                                                              aTTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCg                                                                                  >  2:1557426/1‑150 (MQ=255)
                                                               ttGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGt                                                                                 >  2:3579069/1‑150 (MQ=255)
                                                                 ggAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTAt                                                                               <  1:3796345/150‑1 (MQ=255)
                                                                      gcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCtt                                                                          >  2:570310/1‑150 (MQ=255)
                                                                      gcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCtt                                                                          <  1:2009857/150‑1 (MQ=255)
                                                                       ccGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTa                                                                         <  2:2161848/150‑1 (MQ=255)
                                                                        cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                        >  2:552475/1‑150 (MQ=255)
                                                                        cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                        <  2:3770030/150‑1 (MQ=255)
                                                                        cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                        <  2:1082911/150‑1 (MQ=255)
                                                                         gTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAgg                                                                       >  2:1377617/1‑150 (MQ=255)
                                                                            ggCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGa                                                                    >  2:280722/1‑150 (MQ=255)
                                                                                    aCAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCgg                                                            <  2:1235709/150‑1 (MQ=255)
                                                                                     cAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGc                                                           >  1:2420795/1‑150 (MQ=255)
                                                                                     cAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGc                                                           >  1:2864731/1‑150 (MQ=255)
                                                                                     cAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGc                                                           <  1:3694229/150‑1 (MQ=255)
                                                                                       gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGa                                                         >  1:1310704/1‑150 (MQ=255)
                                                                                       gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGa                                                         >  1:2576961/1‑150 (MQ=255)
                                                                                       gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGa                                                         >  1:760961/1‑150 (MQ=255)
                                                                                         gaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCTCTTAGGCGAGGCTCCGGCGaaa                                                       >  2:2985533/1‑150 (MQ=255)
                                                                                           aaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACt                                                     <  1:401306/150‑1 (MQ=255)
                                                                                            aCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTg                                                    >  1:1311697/1‑150 (MQ=255)
                                                                                             cccTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGc                                                   >  2:3256134/1‑150 (MQ=255)
                                                                                             cccTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGc                                                   >  1:1528589/1‑150 (MQ=255)
                                                                                                ttttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGtt                                                <  2:1965948/150‑1 (MQ=255)
                                                                                                  ttcttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCCCCGGCGAAACTGCGTTGc                                              >  2:2880532/1‑150 (MQ=255)
                                                                                                   tcttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCa                                             >  2:3286119/1‑150 (MQ=255)
                                                                                                    cttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCAc                                            <  1:1902200/150‑1 (MQ=255)
                                                                                                     ttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACt                                           >  1:1166787/1‑150 (MQ=255)
                                                                                                      tcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACtt                                          >  2:788961/1‑150 (MQ=255)
                                                                                                      tcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACtt                                          <  1:252561/150‑1 (MQ=255)
                                                                                                        aCTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCg                                        >  1:58252/1‑150 (MQ=255)
                                                                                                         cTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGt                                       >  1:451476/1‑150 (MQ=255)
                                                                                                          tAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTc                                      >  1:3383390/1‑150 (MQ=255)
                                                                                                          tAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTc                                      <  2:3726351/150‑1 (MQ=255)
                                                                                                             cGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGc                                   >  2:2434954/1‑150 (MQ=255)
                                                                                                                  gggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACa                              >  2:1499616/1‑150 (MQ=255)
                                                                                                                   ggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACAt                             <  2:168694/150‑1 (MQ=255)
                                                                                                                    gCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATg                            <  1:860837/150‑1 (MQ=255)
                                                                                                                    gCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATg                            <  2:77739/150‑1 (MQ=255)
                                                                                                                       gTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGtt                         <  2:1368747/150‑1 (MQ=255)
                                                                                                                         cccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTAc                       >  2:951827/1‑150 (MQ=255)
                                                                                                                         cccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGAGTCTTCTAACTTTTCTGGCGTACAATAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGAGAAACTGCGTTGCACTTCGTCAGCTGACTTGTTTAc                       <  2:313199/150‑1 (MQ=255)
                                                                                                                          ccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACg                      >  1:3272077/1‑150 (MQ=255)
                                                                                                                          ccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACg                      >  2:1634193/1‑150 (MQ=255)
                                                                                                                            ttCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGt                    <  2:3508616/150‑1 (MQ=255)
                                                                                                                            ttCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGt                    <  2:1683150/150‑1 (MQ=255)
                                                                                                                                    cGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAAcc            >  1:3153476/1‑150 (MQ=255)
                                                                                                                                       aGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCaa         >  2:2384589/1‑150 (MQ=255)
                                                                                                                                        gCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAac        <  1:1033728/150‑1 (MQ=255)
                                                                                                                                          ggAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAacac      <  1:570310/150‑1 (MQ=255)
                                                                                                                                           gAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACAcc     >  1:2892303/1‑150 (MQ=255)
                                                                                                                                             aaGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCtt   >  2:2838061/1‑150 (MQ=255)
                                                                                                                                              agagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTa  >  2:1839756/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA  >  CP009273/994181‑994472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑