Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,024,003 C→A D59Y (GAT→TAT)  hspQ ← heat shock protein involved in degradation of mutant DnaA; hemimethylated oriC DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,024,0030CA100.0% 627.5 / NA 188D59Y (GAT→TAT) hspQheat shock protein involved in degradation of mutant DnaA; hemimethylated oriC DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (109/79);  total (109/79)

Only 100 of 194 total aligned reads displayed.

GGGATTAGTTACGCAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCT  >  CP009273/1023856‑1024149
                                                                                                                                                   |                                                                                                                                                  
   aTTAGTTACGCAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGtcct                                                                                                                                               <  1:1531839/150‑1 (MQ=255)
     tAGTTACGCAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGtcctcc                                                                                                                                             >  2:1692235/1‑150 (MQ=255)
        ttACGCAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATc                                                                                                                                          <  1:3766019/150‑1 (MQ=255)
        ttACGCAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATc                                                                                                                                          >  2:3680191/1‑150 (MQ=255)
         tACGCAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCa                                                                                                                                         >  1:2967580/1‑150 (MQ=255)
          aCGCAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCAc                                                                                                                                        <  2:3708942/150‑1 (MQ=255)
             cAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTAc                                                                                                                                     >  1:1380314/1‑150 (MQ=255)
              aGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACg                                                                                                                                    >  2:2419533/1‑150 (MQ=255)
              aGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACg                                                                                                                                    <  2:1161080/150‑1 (MQ=255)
              aGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACg                                                                                                                                    >  2:3583337/1‑150 (MQ=255)
                  gcgGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGAt                                                                                                                                <  1:2419533/150‑1 (MQ=255)
                   cgGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATa                                                                                                                               <  2:1681385/150‑1 (MQ=255)
                    ggggCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATAc                                                                                                                              >  2:394535/1‑150 (MQ=255)
                    ggggCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATAc                                                                                                                              <  1:2627861/150‑1 (MQ=255)
                      ggCCTGGAGTTGTTTGCGGATGGTCTGCGGCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCa                                                                                                                            <  1:3774776/150‑1 (MQ=255)
                      ggCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCa                                                                                                                            <  2:1316447/150‑1 (MQ=255)
                        ccTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACg                                                                                                                          >  2:2622297/1‑150 (MQ=255)
                          tGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGa                                                                                                                        >  1:3491894/1‑150 (MQ=255)
                               ttgttTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGTACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAgcggc                                                                                                                   <  2:106565/150‑1 (MQ=255)
                                tgttTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAgcggcg                                                                                                                  <  2:2543553/150‑1 (MQ=255)
                                tgttTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAgcggcg                                                                                                                  <  1:3214646/150‑1 (MQ=255)
                                tgttTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAgcggcg                                                                                                                  <  1:1123788/150‑1 (MQ=255)
                                tgttTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAgcggcg                                                                                                                  >  1:2935102/1‑150 (MQ=255)
                                    tGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTCGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGaa                                                                                                              <  1:307895/150‑1 (MQ=255)
                                     gCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAg                                                                                                             <  2:462489/150‑1 (MQ=255)
                                        gATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTc                                                                                                          <  2:1131104/150‑1 (MQ=255)
                                              cTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCAtt                                                                                                    >  1:2657739/1‑150 (MQ=255)
                                              cTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCAtt                                                                                                    >  2:1771078/1‑150 (MQ=255)
                                                 cgcCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCAc                                                                                                 >  2:3663275/1‑150 (MQ=255)
                                                    cAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGc                                                                                              >  1:649535/1‑150 (MQ=255)
                                                    cAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGc                                                                                              >  1:2770458/1‑150 (MQ=255)
                                                    cAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGc                                                                                              >  1:2681210/1‑150 (MQ=255)
                                                        tCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGc                                                                                          >  2:189286/1‑150 (MQ=255)
                                                           tCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGACGCTCGTCATTCACCGCCAGCtca                                                                                       >  2:1914794/1‑150 (MQ=255)
                                                               tggatggCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAg                                                                                   >  1:783313/1‑150 (MQ=255)
                                                                    ggCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGAc                                                                              >  1:302347/1‑150 (MQ=255)
                                                                    ggCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGAc                                                                              >  1:1047887/1‑150 (MQ=255)
                                                                    ggCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGAc                                                                              >  2:3713388/1‑150 (MQ=255)
                                                                     gCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACg                                                                             <  1:1055601/150‑1 (MQ=255)
                                                                        gTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGtt                                                                          >  1:3085547/1‑150 (MQ=255)
                                                                         ttCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTc                                                                         <  2:756107/150‑1 (MQ=255)
                                                                          tCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCa                                                                        >  1:2741832/1‑150 (MQ=255)
                                                                           cAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCag                                                                       <  2:3213915/150‑1 (MQ=255)
                                                                             ggATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCagag                                                                     >  2:3037473/1‑150 (MQ=255)
                                                                             ggATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCagag                                                                     >  1:237924/1‑150 (MQ=255)
                                                                             ggATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTCCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCagag                                                                     <  2:1405696/150‑1 (MQ=255)
                                                                               aTGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCagagag                                                                   >  2:3289876/1‑150 (MQ=255)
                                                                                tGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGc                                                                  <  1:312514/150‑1 (MQ=255)
                                                                                tGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGc                                                                  >  1:1910969/1‑150 (MQ=255)
                                                                                 gTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCg                                                                 <  1:2437451/150‑1 (MQ=255)
                                                                                  ttCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGa                                                                <  2:2871933/150‑1 (MQ=255)
                                                                                  ttCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGa                                                                <  2:377428/150‑1 (MQ=255)
                                                                                   tCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGaa                                                               >  2:1534728/1‑150 (MQ=255)
                                                                                    cATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAAt                                                              >  1:3451163/1‑150 (MQ=255)
                                                                                     aTCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATa                                                             >  1:2752842/1‑150 (MQ=255)
                                                                                           cAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCgg                                                       >  2:1096538/1‑150 (MQ=255)
                                                                                              ttCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTc                                                    <  1:1195222/150‑1 (MQ=255)
                                                                                              ttCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTc                                                    <  2:1848701/150‑1 (MQ=255)
                                                                                              ttCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTc                                                    <  2:1876588/150‑1 (MQ=255)
                                                                                               tCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCg                                                   >  2:3644696/1‑150 (MQ=255)
                                                                                                cGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGa                                                  >  2:3432244/1‑150 (MQ=255)
                                                                                                   tACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGatat                                               >  2:130863/1‑150 (MQ=255)
                                                                                                   tACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGatat                                               <  2:1810430/150‑1 (MQ=255)
                                                                                                       tAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCAc                                           <  2:2089502/150‑1 (MQ=255)
                                                                                                       tAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCAc                                           >  2:503652/1‑150 (MQ=255)
                                                                                                       tAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCAc                                           <  1:226208/150‑1 (MQ=255)
                                                                                                        aGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACg                                          >  1:1087896/1‑150 (MQ=255)
                                                                                                        aGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACg                                          >  2:2863671/1‑150 (MQ=255)
                                                                                                          cTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGATTAAACCGGGTCGATATCCACGAc                                        <  1:1791183/150‑1 (MQ=255)
                                                                                                            gggCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCa                                      <  2:762245/150‑1 (MQ=255)
                                                                                                             ggCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCAc                                     >  1:2838922/1‑150 (MQ=255)
                                                                                                              gCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACt                                    >  2:1233744/1‑150 (MQ=255)
                                                                                                                ttCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTcc                                  >  2:3608828/1‑150 (MQ=255)
                                                                                                                 tCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCg                                 <  2:2967580/150‑1 (MQ=255)
                                                                                                                        ggtaggtaTGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAAc                          >  1:394578/1‑150 (MQ=255)
                                                                                                                          taggtaTGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCt                        <  2:783313/150‑1 (MQ=255)
                                                                                                                           aggtaTGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTa                       >  2:139167/1‑150 (MQ=255)
                                                                                                                            ggtaTGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTaa                      >  2:193038/1‑150 (MQ=255)
                                                                                                                             gtaTGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAAc                     >  1:1451987/1‑150 (MQ=255)
                                                                                                                               aTGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAg                   <  2:3520765/150‑1 (MQ=255)
                                                                                                                               aTGAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAg                   >  1:1625516/1‑150 (MQ=255)
                                                                                                                                 gAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGTGTCGATATCCACGACCACTCCGAGATAACCTAACAggg                 <  2:2167448/150‑1 (MQ=255)
                                                                                                                                 gAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAggg                 >  2:1365494/1‑150 (MQ=255)
                                                                                                                                 gAACCGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAggg                 <  2:2349199/150‑1 (MQ=255)
                                                                                                                                    ccGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAAt              >  1:2089583/1‑150 (MQ=255)
                                                                                                                                     cGGTGGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATg             >  1:3578147/1‑150 (MQ=255)
                                                                                                                                     cGGTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATg             >  1:695624/1‑150 (MQ=255)
                                                                                                                                      ggTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATgg            >  1:2441627/1‑150 (MQ=255)
                                                                                                                                       gTAGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGc           <  2:2319134/150‑1 (MQ=255)
                                                                                                                                         aGGCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCgg         >  2:1982770/1‑150 (MQ=255)
                                                                                                                                          ggCCGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGa        >  1:1465683/1‑150 (MQ=255)
                                                                                                                                            ccGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGAcc      >  2:477420/1‑150 (MQ=255)
                                                                                                                                             cGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACct     <  1:2622229/150‑1 (MQ=255)
                                                                                                                                             cGTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACct     <  1:479167/150‑1 (MQ=255)
                                                                                                                                              gTTATAGTCCTCCATCACTACGTGATACCACGTAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctg    >  1:3611549/1‑150 (MQ=255)
                                                                                                                                              gTTATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctg    >  2:2676375/1‑150 (MQ=255)
                                                                                                                                               ttATAGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctgc   >  1:860333/1‑150 (MQ=255)
                                                                                                                                                tataGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctgct  <  2:2364215/150‑1 (MQ=255)
                                                                                                                                                tataGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctgct  >  2:3635531/1‑150 (MQ=255)
                                                                                                                                                tataGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctgct  >  2:3635294/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGATTAGTTACGCAGACGCGGGGCCTGGAGTTGTTTGCGGATGGTCTGCGCCAGCTCATCCATGGATGGCTGTTCAGGATGTTCATCCTGCAATTCGCTACTTAGCTGGGCTTCAGCCAGGTAGGTATGAACCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCT  >  CP009273/1023856‑1024149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑