BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,063,702	G→T	D56Y (GAC→TAC)	ymdF →	KGG family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,063,702	0	G	T	99.5%	719.1 / NA	215	D56Y (GAC→TAC)	ymdF	KGG family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (109/105); minor base A (1/0); total (110/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTG  >  CP009273/1063555‑1063848

ggTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTAc                                                                                                                                                  <  2:2286806/150‑1 (MQ=255)

  ttCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACaa                                                                                                                                                >  1:321662/1‑150 (MQ=255)

  ttCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACaa                                                                                                                                                >  1:323234/1‑150 (MQ=255)

   tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAAc                                                                                                                                               >  1:1975610/1‑150 (MQ=255)

    ccGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACt                                                                                                                                              <  1:379470/150‑1 (MQ=255)

    ccGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACt                                                                                                                                              <  2:2357410/150‑1 (MQ=255)

     cGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTa                                                                                                                                             >  1:1694557/1‑150 (MQ=255)

      ggCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAg                                                                                                                                            >  1:2894855/1‑150 (MQ=255)

         aaTTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCg                                                                                                                                         <  2:3086008/150‑1 (MQ=255)

         aaTTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCg                                                                                                                                         >  2:2471846/1‑150 (MQ=255)

          aTTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCgg                                                                                                                                        <  1:2520831/150‑1 (MQ=255)

           ttttGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCggg                                                                                                                                       <  1:3512636/150‑1 (MQ=255)

             ttGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCt                                                                                                                                     <  2:2634406/150‑1 (MQ=255)

              tGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTa                                                                                                                                    <  1:2004040/150‑1 (MQ=255)

                 agaagaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATc                                                                                                                                 >  2:564058/1‑150 (MQ=255)

                  gaagaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCa                                                                                                                                >  1:2999196/1‑150 (MQ=255)

                    agaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAAt                                                                                                                              <  2:204972/150‑1 (MQ=255)

                       ccGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGAc                                                                                                                           <  1:1377098/150‑1 (MQ=255)

                       ccGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGAc                                                                                                                           <  2:3511754/150‑1 (MQ=255)

                       ccGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGAc                                                                                                                           <  1:3276623/150‑1 (MQ=255)

                        cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACg                                                                                                                          >  1:2691536/1‑150 (MQ=255)

                          cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGaa                                                                                                                        <  2:1220749/150‑1 (MQ=255)

                           gAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCCGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAAt                                                                                                                       >  1:1424589/1‑150 (MQ=255)

                            aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATg                                                                                                                      >  2:3234943/1‑150 (MQ=255)

                            aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATg                                                                                                                      <  2:3575429/150‑1 (MQ=255)

                            aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATg                                                                                                                      >  1:253870/1‑150 (MQ=255)

                             aaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGc                                                                                                                     >  2:1817693/1‑150 (MQ=255)

                              agagCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCa                                                                                                                    >  1:570545/1‑150 (MQ=255)

                              agagCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCa                                                                                                                    >  1:40233/1‑150 (MQ=255)

                                agCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCAtt                                                                                                                  <  1:2186634/150‑1 (MQ=255)

                                 gCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCAttt                                                                                                                 <  2:448221/150‑1 (MQ=255)

                                   aTCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCAttttt                                                                                                               <  1:62931/150‑1 (MQ=255)

                                   aTCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCAttttt                                                                                                               <  2:1447877/150‑1 (MQ=255)

                                      aGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                                                                                                            <  1:2627689/150‑1 (MQ=255)

                                      aGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                                                                                                            >  2:3009189/1‑150 (MQ=255)

                                      aGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                                                                                                            >  1:237493/1‑150 (MQ=255)

                                             ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCt                                                                                                     <  2:3548697/150‑1 (MQ=255)

                                                 aaaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTc                                                                                                 >  2:1712512/1‑150 (MQ=255)

                                                 aaaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTc                                                                                                 >  1:919502/1‑150 (MQ=255)

                                                  aaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCa                                                                                                <  1:3309346/150‑1 (MQ=255)

                                                     aGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaaaa                                                                                             >  2:694541/1‑150 (MQ=255)

                                                       gTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAg                                                                                           >  2:2408151/1‑150 (MQ=255)

                                                       gTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAg                                                                                           <  1:777853/150‑1 (MQ=255)

                                                         ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGcc                                                                                         <  1:2471846/150‑1 (MQ=255)

                                                           acagcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCAt                                                                                       <  1:969876/150‑1 (MQ=255)

                                                             agcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCa                                                                                     >  2:2797820/1‑150 (MQ=255)

                                                               cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                   >  1:1591320/1‑150 (MQ=255)

                                                                acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAccg                                                                                  <  2:3705908/150‑1 (MQ=255)

                                                                  agcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAccgcc                                                                                <  2:3196243/150‑1 (MQ=255)

                                                                  agcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAccgcc                                                                                <  2:3136759/150‑1 (MQ=255)

                                                                  agcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAccgcc                                                                                >  1:116360/1‑150 (MQ=255)

                                                                    cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCgg                                                                              <  1:1818922/150‑1 (MQ=255)

                                                                     gggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                             >  2:1480535/1‑150 (MQ=255)

                                                                      ggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGtt                                                                            >  2:388633/1‑150 (MQ=255)

                                                                         gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                         >  1:2109405/1‑150 (MQ=255)

                                                                          tAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAccc                                                                        <  1:140177/150‑1 (MQ=255)

                                                                             tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggt                                                                     >  2:3692559/1‑150 (MQ=255)

                                                                                 aaGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGtt                                                                 <  2:514870/150‑1 (MQ=255)

                                                                                 aaGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGtt                                                                 >  2:2217748/1‑150 (MQ=255)

                                                                                 aaGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGtt                                                                 >  1:1904171/1‑150 (MQ=255)

                                                                                    aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGAt                                                              >  1:1106605/1‑150 (MQ=255)

                                                                                    aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGAt                                                              >  1:2730279/1‑150 (MQ=255)

                                                                                       gACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACt                                                           <  1:3753493/150‑1 (MQ=255)

                                                                                          ccGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgat                                                        <  1:1977450/150‑1 (MQ=255)

                                                                                              aGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACa                                                    >  1:1001666/1‑150 (MQ=255)

                                                                                              aGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACa                                                    >  1:682729/1‑150 (MQ=255)

                                                                                                  cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATg                                                >  2:1805140/1‑150 (MQ=255)

                                                                                                   gcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGt                                               >  1:3371542/1‑150 (MQ=255)

                                                                                                    cATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTa                                              <  2:78264/150‑1 (MQ=255)

                                                                                                     aTCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                             <  1:156291/150‑1 (MQ=255)

                                                                                                      tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                            >  1:1246481/1‑150 (MQ=255)

                                                                                                      tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                            >  1:3630295/1‑150 (MQ=255)

                                                                                                        tGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCt                                          <  1:1415891/150‑1 (MQ=255)

                                                                                                         gAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCtt                                         <  1:216593/150‑1 (MQ=255)

                                                                                                         gAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCtt                                         >  2:73331/1‑150 (MQ=255)

                                                                                                         gAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCtt                                         <  1:3018173/150‑1 (MQ=255)

                                                                                                             cAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCt                                     >  1:458009/1‑150 (MQ=255)

                                                                                                              aGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                    <  1:2403071/150‑1 (MQ=255)

                                                                                                              aGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                    >  1:225966/1‑150 (MQ=255)

                                                                                                              aGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                    >  1:1049979/1‑150 (MQ=255)

                                                                                                                  aaaaaaGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgc                                >  2:2693311/1‑150 (MQ=255)

                                                                                                                  aaaaaaGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgc                                >  2:1607596/1‑150 (MQ=255)

                                                                                                                   aaaaaGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                               <  1:100983/150‑1 (MQ=255)

                                                                                                                   aaaaaGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                               >  1:1466705/1‑150 (MQ=255)

                                                                                                                   aaaaaGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                               >  2:250247/1‑150 (MQ=255)

                                                                                                                     aaaGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATCCTGATGACAAATGTAAGCTTGCCTGATGcgcg                             >  2:1048564/1‑150 (MQ=255)

                                                                                                                        ggtggtAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                          <  2:886096/150‑1 (MQ=255)

                                                                                                                            gtAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                      <  1:3555234/150‑1 (MQ=255)

                                                                                                                                gagCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAg                  <  2:1975610/150‑1 (MQ=255)

                                                                                                                                  gCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGc                >  1:3137589/1‑150 (MQ=255)

                                                                                                                                   cAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGcc               <  1:2279034/150‑1 (MQ=255)

                                                                                                                                      tCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc            >  1:2756435/1‑150 (MQ=255)

                                                                                                                                       cACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAcc           <  1:3692559/150‑1 (MQ=255)

                                                                                                                                          ggCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCaga        >  2:1460122/1‑150 (MQ=255)

                                                                                                                                           gCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa       >  2:3206062/1‑150 (MQ=255)

                                                                                                                                            cAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaag      <  1:977580/150‑1 (MQ=255)

                                                                                                                                            cAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaag      <  2:3371542/150‑1 (MQ=255)

                                                                                                                                            cAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaag      <  2:2894855/150‑1 (MQ=255)

                                                                                                                                            cAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaag      <  2:2999196/150‑1 (MQ=255)

                                                                                                                                            cAAAAGCTACAACTAGCCGGGCTAATAAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaag      >  1:117116/1‑150 (MQ=255)

GGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTG  >  CP009273/1063555‑1063848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`