Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,077,911 A→C K71N (AAA→AAC)  efeO → inactive ferrous ion transporter EfeUOB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,077,9110AC100.0% 790.1 / NA 199K71N (AAA→AAC) efeOinactive ferrous ion transporter EfeUOB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (93/106);  total (93/106)

Only 100 of 199 total aligned reads displayed.

TATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCGTCAAAGGTGAGGCAAC  >  CP009273/1077764‑1078057
                                                                                                                                                   |                                                                                                                                                  
tATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACgg                                                                                                                                                  <  1:511152/150‑1 (MQ=255)
 aTGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCCGAAGGCGCTGGAGTGGGAGATCCTCAACGGc                                                                                                                                                 >  2:3415610/1‑150 (MQ=255)
  tGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCg                                                                                                                                                >  1:2712485/1‑150 (MQ=255)
   ggCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGt                                                                                                                                               >  1:356293/1‑150 (MQ=255)
     cTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGa                                                                                                                                             >  1:1264355/1‑150 (MQ=255)
      tAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGAt                                                                                                                                            <  2:624634/150‑1 (MQ=255)
        aCGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGAtgg                                                                                                                                          <  1:1533020/150‑1 (MQ=255)
        aCGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGAtgg                                                                                                                                          >  1:3623802/1‑150 (MQ=255)
         cgccgcTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGAtggt                                                                                                                                         <  1:3267454/150‑1 (MQ=255)
            cgcTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGAtggtggt                                                                                                                                      <  2:348373/150‑1 (MQ=255)
              cTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGAtggtggtgg                                                                                                                                    <  1:1788895/150‑1 (MQ=255)
               tGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGa                                                                                                                                   >  2:942312/1‑150 (MQ=255)
                    tgCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGc                                                                                                                              <  1:943818/150‑1 (MQ=255)
                     gCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCg                                                                                                                             <  2:2772012/150‑1 (MQ=255)
                         cAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGaa                                                                                                                         >  2:1230538/1‑150 (MQ=255)
                             tCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAata                                                                                                                     <  1:3686314/150‑1 (MQ=255)
                             tCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAata                                                                                                                     <  1:597573/150‑1 (MQ=255)
                             tCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAata                                                                                                                     >  2:2417653/1‑150 (MQ=255)
                             tCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAata                                                                                                                     <  1:2503719/150‑1 (MQ=255)
                               aaaGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATc                                                                                                                   >  1:1845167/1‑150 (MQ=255)
                               aaaGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATc                                                                                                                   <  2:2812489/150‑1 (MQ=255)
                                aaGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCg                                                                                                                  <  1:2666667/150‑1 (MQ=255)
                                 aGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGc                                                                                                                 >  1:731276/1‑150 (MQ=255)
                                 aGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGc                                                                                                                 <  1:1557148/150‑1 (MQ=255)
                                          gACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCtt                                                                                                        <  1:2993540/150‑1 (MQ=255)
                                            cGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTa                                                                                                      >  1:1410949/1‑150 (MQ=255)
                                             ggATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAg                                                                                                     <  1:3661772/150‑1 (MQ=255)
                                             ggATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGAGGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAg                                                                                                     >  2:312284/1‑150 (MQ=255)
                                               aTAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGcc                                                                                                   >  1:2452563/1‑150 (MQ=255)
                                                      gTGCGACCCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCGGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGaaact                                                                                            <  1:3200771/150‑3 (MQ=255)
                                                         cGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGAc                                                                                         <  1:768301/150‑1 (MQ=255)
                                                         cGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGAc                                                                                         >  2:2974435/1‑150 (MQ=255)
                                                          gAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACg                                                                                        >  1:3468535/1‑150 (MQ=255)
                                                          gAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACg                                                                                        >  1:166764/1‑150 (MQ=255)
                                                           aaCCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACgg                                                                                       <  1:3711357/150‑1 (MQ=255)
                                                              cGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGa                                                                                    >  1:1822748/1‑150 (MQ=255)
                                                                aTGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAAt                                                                                  >  2:1803593/1‑150 (MQ=255)
                                                                 tGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAAtt                                                                                 >  1:3383115/1‑150 (MQ=255)
                                                                 tGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAAtt                                                                                 <  2:2452563/150‑1 (MQ=255)
                                                                    ccATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTAc                                                                              >  2:2815321/1‑150 (MQ=255)
                                                                    ccATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTAc                                                                              <  1:3034906/150‑1 (MQ=255)
                                                                      aTTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAg                                                                            <  2:542279/150‑1 (MQ=255)
                                                                       ttACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGc                                                                           >  2:2383568/1‑150 (MQ=255)
                                                                       ttACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGc                                                                           <  2:1865044/150‑1 (MQ=255)
                                                                         aCGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCt                                                                         <  2:1454636/150‑1 (MQ=255)
                                                                          cGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTg                                                                        <  1:317881/150‑1 (MQ=255)
                                                                          cGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTg                                                                        <  1:1829184/150‑1 (MQ=255)
                                                                          cGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTg                                                                        <  1:935706/150‑1 (MQ=255)
                                                                          cGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTg                                                                        >  1:2785123/1‑150 (MQ=255)
                                                                            gTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGc                                                                      <  2:2076661/150‑1 (MQ=255)
                                                                             ttAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCg                                                                     <  2:3609065/150‑1 (MQ=255)
                                                                              tAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGa                                                                    <  2:1903386/150‑1 (MQ=255)
                                                                              tAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGa                                                                    <  1:3111941/150‑1 (MQ=255)
                                                                               aaCGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGaa                                                                   >  2:2012239/1‑150 (MQ=255)
                                                                                aCGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAAt                                                                  <  1:2848470/150‑1 (MQ=255)
                                                                                 cGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATa                                                                 <  2:3367837/150‑1 (MQ=255)
                                                                                 cGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATa                                                                 <  1:3756663/150‑1 (MQ=255)
                                                                                 cGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATa                                                                 >  2:445340/1‑150 (MQ=255)
                                                                                     gggAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGat                                                             <  2:448199/150‑1 (MQ=255)
                                                                                     gggAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGat                                                             <  2:449530/150‑1 (MQ=255)
                                                                                       gAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGatat                                                           >  1:2473550/1‑150 (MQ=255)
                                                                                       gAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGatat                                                           >  1:1202130/1‑150 (MQ=255)
                                                                                        aaaaCACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATg                                                          >  2:1360039/1‑150 (MQ=255)
                                                                                        aaaaCACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATg                                                          >  1:1376210/1‑150 (MQ=255)
                                                                                         aaaCACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGa                                                         >  2:1577264/1‑150 (MQ=255)
                                                                                          aaCACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCAGGCATTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGAc                                                        >  2:1907128/1‑150 (MQ=255)
                                                                                           acacAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGAcc                                                       <  2:2104208/150‑1 (MQ=255)
                                                                                            cacaGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCt                                                      <  2:2881585/150‑1 (MQ=255)
                                                                                            cacaGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCt                                                      <  2:1106100/150‑1 (MQ=255)
                                                                                            cacaGTTCATTATTCAGAACCACAGCAAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAATAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCt                                                      >  1:1328465/1‑150 (MQ=255)
                                                                                              caGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGc                                                    >  1:3207047/1‑150 (MQ=255)
                                                                                              caGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGc                                                    >  1:1971805/1‑150 (MQ=255)
                                                                                                 ttCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGt                                                 >  2:2785437/1‑150 (MQ=255)
                                                                                                   cATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTct                                               <  1:2530221/150‑1 (MQ=255)
                                                                                                    attattCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTctg                                              <  2:2151942/150‑1 (MQ=255)
                                                                                                      tattCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTctgct                                            <  1:1076409/150‑1 (MQ=255)
                                                                                                       attCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTctgctg                                           <  1:2437262/150‑1 (MQ=255)
                                                                                                       attCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTctgctg                                           <  2:3684196/150‑1 (MQ=255)
                                                                                                           aGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTa                                       >  2:3520867/1‑150 (MQ=255)
                                                                                                            gAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTaa                                      <  2:2327314/150‑1 (MQ=255)
                                                                                                               ccACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAAccc                                   <  2:2712485/150‑1 (MQ=255)
                                                                                                               ccACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAAccc                                   <  2:2258666/150‑1 (MQ=255)
                                                                                                               ccACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAAccc                                   <  1:494937/150‑1 (MQ=255)
                                                                                                                 acaGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGa                                 <  1:484339/150‑1 (MQ=255)
                                                                                                                   aGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGaaa                               <  1:935269/150‑1 (MQ=255)
                                                                                                                   aGCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGaaa                               >  2:753548/1‑150 (MQ=255)
                                                                                                                    gCCAGAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAg                              >  1:1725052/1‑150 (MQ=255)
                                                                                                                        gAAGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGaa                          >  1:2212838/1‑150 (MQ=255)
                                                                                                                          aGGCGCTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGt                        >  1:2971318/1‑150 (MQ=255)
                                                                                                                             cgcTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGa                     >  2:1183439/1‑150 (MQ=255)
                                                                                                                               cTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATc                   <  1:2095141/150‑1 (MQ=255)
                                                                                                                               cTGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATc                   >  1:2291784/1‑150 (MQ=255)
                                                                                                                                tGGAGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCg                  <  1:2911898/150‑1 (MQ=255)
                                                                                                                                   aGTGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCGTCa               >  2:1653572/1‑150 (MQ=255)
                                                                                                                                     tGGGAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGAGATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCGTCaaa             >  2:2474768/1‑150 (MQ=255)
                                                                                                                                      gggAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCGTCAAAg            <  2:2810049/150‑1 (MQ=255)
                                                                                                                                      gggAGATCCTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCGTCAAAg            <  2:1710153/150‑1 (MQ=255)
                                                                                                                                              cTCAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCGTCAAAGGTGAGGCa    <  1:179555/150‑1 (MQ=255)
                                                                                                                                                cAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGACGTTGATCGTCAAAGGTGAGGCAAc  >  2:89863/1‑150 (MQ=255)
                                                                                                                                                cAACGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCGTCAAAGGTGAGGCAAc  <  1:578727/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTCTGCTGACTAACCCGAAAGGGAAGTTGATCGTCAAAGGTGAGGCAAC  >  CP009273/1077764‑1078057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑