Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,084,103 A→T L404* (TTA→TAA)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,084,1030AT100.0% 473.2 / NA 143L404* (TTA→TAA) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (75/68);  total (75/68)

Only 100 of 145 total aligned reads displayed.

AGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAAT  >  CP009273/1083956‑1084249
                                                                                                                                                   |                                                                                                                                                  
 gATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTtata                                                                                                                                                 <  2:506928/150‑1 (MQ=255)
  aTCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAc                                                                                                                                                >  1:2934750/1‑150 (MQ=255)
  aTCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAc                                                                                                                                                <  2:49684/150‑1 (MQ=255)
  aTCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAc                                                                                                                                                >  1:3537786/1‑150 (MQ=255)
   tCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAAGTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAcc                                                                                                                                               <  2:2292985/150‑1 (MQ=255)
        atatAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCAt                                                                                                                                          >  1:12256/1‑150 (MQ=255)
          ataACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATcc                                                                                                                                        >  2:2432727/1‑150 (MQ=255)
            aaCATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCAc                                                                                                                                      >  2:916247/1‑150 (MQ=255)
               aTGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCa                                                                                                                                   >  2:3132726/1‑150 (MQ=255)
                tGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCAt                                                                                                                                  <  1:2969311/150‑1 (MQ=255)
                 gCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATa                                                                                                                                 >  1:3598252/1‑150 (MQ=255)
                   cAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGa                                                                                                                               >  2:1502845/1‑150 (MQ=255)
                     aCTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATg                                                                                                                             <  1:1701813/150‑1 (MQ=255)
                           gCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTaca                                                                                                                       <  2:1517887/150‑1 (MQ=255)
                            ctctGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTacac                                                                                                                      <  1:2484890/150‑1 (MQ=255)
                            ctctGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTacac                                                                                                                      >  1:2007539/1‑150 (MQ=255)
                               tGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTg                                                                                                                   >  1:356553/1‑150 (MQ=255)
                                gACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGa                                                                                                                  <  1:3801947/150‑1 (MQ=255)
                                       tCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTa                                                                                                           <  1:1332937/150‑1 (MQ=255)
                                       tCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTa                                                                                                           <  2:1478146/150‑1 (MQ=255)
                                         aTCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACg                                                                                                         <  1:2362323/150‑1 (MQ=255)
                                         aTCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACg                                                                                                         >  2:2226587/1‑150 (MQ=255)
                                         aTCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACg                                                                                                         >  2:856998/1‑150 (MQ=255)
                                           tgAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTa                                                                                                       >  1:241661/2‑150 (MQ=255)
                                            gAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTaa                                                                                                      >  1:218602/1‑150 (MQ=255)
                                             aaaGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAAt                                                                                                     >  2:87825/1‑150 (MQ=255)
                                               aGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTg                                                                                                   >  1:1632214/1‑150 (MQ=255)
                                               aGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTg                                                                                                   <  1:3034681/150‑1 (MQ=255)
                                               aGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTg                                                                                                   <  2:3537786/150‑1 (MQ=255)
                                               aGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTg                                                                                                   >  1:1551309/1‑150 (MQ=255)
                                                 gagagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGcc                                                                                                 <  1:2513852/150‑1 (MQ=255)
                                                 gagagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGcc                                                                                                 <  2:2142231/150‑1 (MQ=255)
                                                  agagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCa                                                                                                <  2:1024062/150‑1 (MQ=255)
                                                   gagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg                                                                                               <  1:1956835/150‑1 (MQ=255)
                                                    agagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAgg                                                                                              <  2:1914601/150‑1 (MQ=255)
                                                    agagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAgg                                                                                              <  2:607558/150‑1 (MQ=255)
                                                    agagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAgg                                                                                              >  2:2634902/1‑150 (MQ=255)
                                                     gagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGc                                                                                             <  2:698603/150‑1 (MQ=255)
                                                      agaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCa                                                                                            <  1:1160001/150‑1 (MQ=255)
                                                        aCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAc                                                                                          <  2:3441134/150‑1 (MQ=255)
                                                        aCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAc                                                                                          <  2:3441114/150‑1 (MQ=255)
                                                         cGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTCATTGCCAGGCAAcc                                                                                         >  2:1283930/1‑150 (MQ=255)
                                                           gTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCg                                                                                       <  1:3087858/150‑1 (MQ=255)
                                                             gATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGAc                                                                                     >  1:3306021/1‑150 (MQ=255)
                                                                atTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTaa                                                                                  <  1:2754641/150‑1 (MQ=255)
                                                                atTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTAAGCGGGTACGTAATTGCCAGGCAACCCGACTaa                                                                                  >  1:2530047/1‑150 (MQ=255)
                                                                atTGTTCAGGATGAACTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTaa                                                                                  <  1:1685886/150‑1 (MQ=255)
                                                                 ttGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTaaa                                                                                 <  2:926406/150‑1 (MQ=255)
                                                                    ttCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAat                                                                              <  2:2934750/150‑1 (MQ=255)
                                                                      cAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAatat                                                                            >  2:3523041/1‑150 (MQ=255)
                                                                      cAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAatat                                                                            <  2:2615453/150‑1 (MQ=255)
                                                                        ggATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCt                                                                          >  2:582355/1‑150 (MQ=255)
                                                                          aTGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGc                                                                        >  1:2792405/1‑150 (MQ=255)
                                                                           tGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCt                                                                       >  2:297575/1‑150 (MQ=255)
                                                                               tttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCa                                                                   >  1:3527359/1‑150 (MQ=255)
                                                                               tttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCa                                                                   >  1:3527869/1‑150 (MQ=255)
                                                                                ttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAt                                                                  <  1:760968/150‑1 (MQ=255)
                                                                                ttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAt                                                                  >  2:736619/1‑150 (MQ=255)
                                                                                 tgtgCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAtt                                                                 <  2:3327465/150‑1 (MQ=255)
                                                                                   tgCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTgg                                                               <  1:2628166/150‑1 (MQ=255)
                                                                                      ttttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAg                                                            <  2:1886242/150‑1 (MQ=255)
                                                                                        ttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCa                                                          <  1:2600153/150‑1 (MQ=255)
                                                                                        ttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCa                                                          >  1:1228108/1‑150 (MQ=255)
                                                                                        ttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCa                                                          <  2:3598252/150‑1 (MQ=255)
                                                                                         tttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCaa                                                         <  2:1705452/150‑1 (MQ=255)
                                                                                         tttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCaa                                                         >  1:3154574/1‑150 (MQ=255)
                                                                                           tctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAAc                                                       >  1:1009282/1‑150 (MQ=255)
                                                                                            ctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACg                                                      >  2:748259/1‑150 (MQ=255)
                                                                                             tcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGa                                                     >  2:2448735/1‑150 (MQ=255)
                                                                                              ccccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAt                                                    >  2:2405030/1‑150 (MQ=255)
                                                                                                ccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAttt                                                  >  2:458967/1‑150 (MQ=255)
                                                                                                 cTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTg                                                 >  2:723504/1‑150 (MQ=255)
                                                                                                   gTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGa                                               >  1:353991/1‑150 (MQ=255)
                                                                                                   gTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGa                                               >  1:674827/1‑150 (MQ=255)
                                                                                                     tGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGaaa                                             >  2:2527153/1‑150 (MQ=255)
                                                                                                      ggTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAAc                                            >  2:1770146/1‑150 (MQ=255)
                                                                                                       gTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAAcc                                           >  1:18166/1‑150 (MQ=255)
                                                                                                        tAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCa                                          <  1:87825/150‑1 (MQ=255)
                                                                                                          aGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGa                                        <  1:1331083/150‑1 (MQ=255)
                                                                                                            tATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGAcc                                      <  2:1199721/150‑1 (MQ=255)
                                                                                                                ttACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCtt                                  <  2:302786/150‑1 (MQ=255)
                                                                                                                ttACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCtt                                  >  1:2253314/1‑150 (MQ=255)
                                                                                                                 tACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCttt                                 <  2:3221923/150‑1 (MQ=255)
                                                                                                                 tACTCGCGTTAATGTGGGAACTAAATCCCAGCTTTATACCTGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTTCCATGCAACCCGACTAAAAATATCTGCTTTCATAGGTAGCAAACGATTTTGAAACCATACCTCttt                                 >  1:2852749/1‑150 (MQ=255)
                                                                                                                 tACTCGAGTTAATGTGGGATATAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTAAGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCttt                                 <  1:2973475/150‑1 (MQ=255)
                                                                                                                  aCTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTg                                >  1:2344456/1‑150 (MQ=255)
                                                                                                                           aaTGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCa                       >  1:2678695/1‑150 (MQ=255)
                                                                                                                             tgtgGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATc                     <  2:3701705/150‑1 (MQ=255)
                                                                                                                             tgtgGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATc                     <  2:3702175/150‑1 (MQ=255)
                                                                                                                              gtgGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCa                    >  2:1359030/1‑150 (MQ=255)
                                                                                                                                gggATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCAcc                  <  2:1723929/150‑1 (MQ=255)
                                                                                                                                 ggATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCa                 >  2:1317075/1‑150 (MQ=255)
                                                                                                                                     cTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCgg             >  1:489820/1‑150 (MQ=255)
                                                                                                                                      tAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCggg            <  1:2861006/150‑1 (MQ=255)
                                                                                                                                       aaaTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGa           <  2:427176/150‑1 (MQ=255)
                                                                                                                                       aaaTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGa           <  2:427161/150‑1 (MQ=255)
                                                                                                                                          tCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCa        >  1:3770081/1‑150 (MQ=255)
                                                                                                                                          tCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCa        <  2:12256/150‑1 (MQ=255)
                                                                                                                                              aGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCaa    >  1:1110623/1‑150 (MQ=255)
                                                                                                                                                cTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAAt  >  1:3711543/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAAT  >  CP009273/1083956‑1084249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑