Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,106,503 C→A S62* (TCA→TAA)  opgH → membrane glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,106,5030CA100.0% 836.3 / NA 253S62* (TCA→TAA) opgHmembrane glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (112/141);  total (112/141)

Only 100 of 253 total aligned reads displayed.

TCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATG  >  CP009273/1106356‑1106649
                                                                                                                                                   |                                                                                                                                                  
  gccgcAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACtt                                                                                                                                                <  2:2776587/150‑1 (MQ=255)
     gcAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCt                                                                                                                                             <  2:2932621/150‑1 (MQ=255)
      cAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTg                                                                                                                                            >  2:822512/1‑150 (MQ=255)
        aGCGAGAGAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGAt                                                                                                                                          >  1:2879511/1‑150 (MQ=255)
        aGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGAt                                                                                                                                          >  1:3239983/1‑150 (MQ=255)
         gCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATg                                                                                                                                         <  1:769849/150‑1 (MQ=255)
            agaAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGAc                                                                                                                                      <  1:3277780/150‑1 (MQ=255)
             gaAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACa                                                                                                                                     <  1:2975035/150‑1 (MQ=255)
              aaaGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAg                                                                                                                                    >  2:2114286/1‑150 (MQ=255)
                  cGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGttaa                                                                                                                                <  1:2890950/150‑1 (MQ=255)
                    gCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCAGGCCAGATTAACTTGCTGATGGACAGttaatt                                                                                                                              >  2:3024460/1‑150 (MQ=255)
                     cATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGttaatta                                                                                                                             <  1:1155495/150‑1 (MQ=255)
                      aTTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGttaattaa                                                                                                                            >  2:1585456/1‑150 (MQ=255)
                        tGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAg                                                                                                                          >  2:1089579/1‑150 (MQ=255)
                         gCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAga                                                                                                                         <  2:3238565/150‑1 (MQ=255)
                          ccGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAgac                                                                                                                        <  2:630158/150‑1 (MQ=255)
                           cGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAgacg                                                                                                                       <  1:1035958/150‑1 (MQ=255)
                           cGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAgacg                                                                                                                       <  1:934416/150‑1 (MQ=255)
                            gAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAgacga                                                                                                                      <  2:471776/150‑1 (MQ=255)
                            gAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAgacga                                                                                                                      >  2:3087185/1‑150 (MQ=255)
                              aGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGacgacg                                                                                                                    <  1:1294735/150‑1 (MQ=255)
                                aCTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGaa                                                                                                                  <  1:306101/150‑1 (MQ=255)
                                aCTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGaa                                                                                                                  >  2:3716174/1‑150 (MQ=255)
                                 cTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAg                                                                                                                 <  1:2376661/150‑1 (MQ=255)
                                 cTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAg                                                                                                                 <  1:1393445/150‑1 (MQ=255)
                                  tGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAgg                                                                                                                <  1:1118074/150‑1 (MQ=255)
                                    atatCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGgc                                                                                                              >  2:3596546/1‑150 (MQ=255)
                                      atCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGgcgc                                                                                                            <  1:2277489/150‑1 (MQ=255)
                                           cgcCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCa                                                                                                       <  2:1701107/150‑1 (MQ=255)
                                              cGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCt                                                                                                    <  1:1934035/150‑1 (MQ=255)
                                               gTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTg                                                                                                   >  1:118222/1‑150 (MQ=255)
                                                   atcaGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAgg                                                                                               >  2:2371766/1‑150 (MQ=255)
                                                    tcaGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGc                                                                                              >  1:3097974/1‑150 (MQ=255)
                                                    tcaGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGc                                                                                              >  1:1197323/1‑150 (MQ=255)
                                                     caGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCg                                                                                             >  1:1799213/1‑150 (MQ=255)
                                                       ggCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGAt                                                                                           <  2:1329084/150‑1 (MQ=255)
                                                        gcgcTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATg                                                                                          <  1:3474443/150‑1 (MQ=255)
                                                          gcTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGcc                                                                                        >  1:950466/1‑150 (MQ=255)
                                                          gcTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGcc                                                                                        <  2:887659/150‑1 (MQ=255)
                                                          gcTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGcc                                                                                        >  1:1585000/1‑150 (MQ=255)
                                                            tGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAg                                                                                      >  2:2541253/1‑150 (MQ=255)
                                                              gATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGaa                                                                                    >  2:1237023/1‑150 (MQ=255)
                                                               aTGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGACCAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAg                                                                                   >  2:3283731/1‑150 (MQ=255)
                                                                          cGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGctcc                                                                        <  1:1272652/150‑1 (MQ=255)
                                                                          cGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGctcc                                                                        >  1:2951802/1‑150 (MQ=255)
                                                                          cGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGctcc                                                                        >  2:1443973/1‑150 (MQ=255)
                                                                          cGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGctcc                                                                        >  2:1444607/1‑150 (MQ=255)
                                                                            cACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGctcctc                                                                      <  1:1702972/150‑1 (MQ=255)
                                                                            cACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGctcctc                                                                      <  2:3332269/150‑1 (MQ=255)
                                                                             aCCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCg                                                                     <  2:3049467/150‑1 (MQ=255)
                                                                               cTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTATAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTATAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGAt                                                                   <  1:2827141/150‑1 (MQ=255)
                                                                                 gggCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGt                                                                 >  1:2053386/1‑150 (MQ=255)
                                                                                 gggCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGt                                                                 <  2:2360678/150‑1 (MQ=255)
                                                                                    cgcgGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTc                                                              <  2:1790225/150‑1 (MQ=255)
                                                                                     gcgGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTATCTTGCTGATGGACTGTTAATTAAAGACGACGAAGGGAGCGATCAGCTGATGGCGATGACAGTAGCAAAACGCTCCTCGATGTTTcc                                                             <  2:2867071/150‑1 (MQ=255)
                                                                                     gcgGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTcc                                                             <  2:2408230/150‑1 (MQ=255)
                                                                                     gcgGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTcc                                                             <  2:690542/150‑1 (MQ=255)
                                                                                     gcgGGAGGATGAGTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTcc                                                             <  2:1587466/150‑1 (MQ=255)
                                                                                          aggaTGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGAcc                                                        >  1:3580507/1‑150 (MQ=255)
                                                                                              tgatTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTg                                                    <  2:3797060/150‑1 (MQ=255)
                                                                                               gatTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTgg                                                   >  1:1462452/1‑150 (MQ=255)
                                                                                                atTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGc                                                  >  1:803232/1‑150 (MQ=255)
                                                                                                   ccccGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTa                                               <  1:408774/150‑1 (MQ=255)
                                                                                                     ccGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTAcc                                             <  2:2422041/150‑1 (MQ=255)
                                                                                                     ccGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACGTGCTGAGGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTAcc                                             <  2:2879511/150‑1 (MQ=255)
                                                                                                      cGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTCCCa                                            >  2:2582133/1‑150 (MQ=255)
                                                                                                       gCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCaa                                           <  2:2241656/150‑1 (MQ=255)
                                                                                                       gCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCaa                                           >  2:3537069/1‑150 (MQ=255)
                                                                                                        cAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAAc                                          >  1:2294231/1‑150 (MQ=255)
                                                                                                         aaGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAAcc                                         >  2:1680920/1‑150 (MQ=255)
                                                                                                                 gTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGc                                 >  2:2073462/1‑150 (MQ=255)
                                                                                                                 gTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGc                                 >  2:3484548/1‑150 (MQ=255)
                                                                                                                   aaaGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCg                               >  2:2807616/1‑150 (MQ=255)
                                                                                                                    aaGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGt                              >  1:2780837/1‑150 (MQ=255)
                                                                                                                     aGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGtt                             <  2:1848494/150‑1 (MQ=255)
                                                                                                                     aGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGtt                             >  2:1918943/1‑150 (MQ=255)
                                                                                                                        cgcgTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCt                          <  1:3183475/150‑1 (MQ=255)
                                                                                                                         gcgTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTg                         <  2:1452437/150‑1 (MQ=255)
                                                                                                                          cgTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTgg                        >  2:1480028/1‑150 (MQ=255)
                                                                                                                          cgTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTgg                        >  2:76914/1‑150 (MQ=255)
                                                                                                                            tCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGa                      <  1:3769186/150‑1 (MQ=255)
                                                                                                                              tGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATc                    <  1:822512/150‑1 (MQ=255)
                                                                                                                              tGGAACAAGCCTGGCCAGATTAACTCGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATc                    >  1:2608834/1‑150 (MQ=255)
                                                                                                                                gAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGc                  >  2:3300340/1‑150 (MQ=255)
                                                                                                                                gAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGc                  >  2:3770764/1‑150 (MQ=255)
                                                                                                                                   cAAGCCTGGCCAGATGAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTg               <  2:2915380/150‑1 (MQ=255)
                                                                                                                                      gCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGt            >  1:1057973/1‑150 (MQ=255)
                                                                                                                                      gCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGt            >  1:2958872/1‑150 (MQ=255)
                                                                                                                                        cTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTgg          >  1:1610528/1‑150 (MQ=255)
                                                                                                                                          ggCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAc        <  1:2508592/150‑1 (MQ=255)
                                                                                                                                          ggCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAc        <  1:2821363/150‑1 (MQ=255)
                                                                                                                                          ggCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAc        <  1:3429837/150‑1 (MQ=255)
                                                                                                                                          ggCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAc        <  1:603781/150‑1 (MQ=255)
                                                                                                                                             cAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAcgcg     >  2:2168022/1‑150 (MQ=255)
                                                                                                                                             cAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAcgcg     >  2:1870261/1‑150 (MQ=255)
                                                                                                                                             cAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAcgcg     <  1:542321/150‑1 (MQ=255)
                                                                                                                                             cAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAcgcg     >  1:2346906/1‑150 (MQ=255)
                                                                                                                                              aGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGa    >  2:2284892/1‑150 (MQ=255)
                                                                                                                                               gATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGAt   >  1:2225600/1‑150 (MQ=255)
                                                                                                                                                aTTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATg  >  2:3463301/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATG  >  CP009273/1106356‑1106649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑