Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,133,311 T→A L162Q (CTG→CAG)  flgJ → flagellar rod assembly protein and murein hydrolase; flagellum‑specific muramidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,133,3110TA99.5% 683.7 / NA 209L162Q (CTG→CAG) flgJflagellar rod assembly protein and murein hydrolase; flagellum‑specific muramidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (124/84);  minor base C (0/1);  total (124/85)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

CGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACTGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAG  >  CP009273/1133164‑1133457
                                                                                                                                                   |                                                                                                                                                  
    gtaaTTCCCGCTCGACCCTGTGGTGCGTTATCAAAATCAGGCGCGTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCAGTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAg                                                                                                                                              <  2:632169/148‑1 (MQ=255)
      aaTTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGcc                                                                                                                                            >  2:1702079/1‑150 (MQ=255)
       aTTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCa                                                                                                                                           >  2:421120/1‑150 (MQ=255)
        ttCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAg                                                                                                                                          >  2:1523884/1‑150 (MQ=255)
         tCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGc                                                                                                                                         <  1:10395/150‑1 (MQ=255)
              cTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGc                                                                                                                                    >  2:1737400/1‑150 (MQ=255)
                cGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCgg                                                                                                                                  >  2:1243050/1‑150 (MQ=255)
                 gAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGgt                                                                                                                                 >  2:1573502/1‑150 (MQ=255)
                  aaaCTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGgtg                                                                                                                                >  1:2158116/1‑150 (MQ=255)
                  aaaCTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGgtg                                                                                                                                >  1:3318725/1‑150 (MQ=255)
                   aaCTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGgtgt                                                                                                                               <  1:1693960/150‑1 (MQ=255)
                    aCTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGtgtg                                                                                                                              <  1:1490963/150‑1 (MQ=255)
                    aCTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGtgtg                                                                                                                              <  1:3389025/150‑1 (MQ=255)
                    aCTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGtgtg                                                                                                                              <  1:1991835/150‑1 (MQ=255)
                    aCTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGtgtg                                                                                                                              >  1:155414/1‑150 (MQ=255)
                     cTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGc                                                                                                                             >  2:1306352/1‑150 (MQ=255)
                     cTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGc                                                                                                                             >  2:3206620/1‑150 (MQ=255)
                       gtggtgCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCca                                                                                                                           >  2:1442433/1‑150 (MQ=255)
                        tggtgCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCcac                                                                                                                          >  2:3040765/1‑150 (MQ=255)
                          gtgCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCAcat                                                                                                                        >  1:1975008/1‑150 (MQ=255)
                          gtgCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCAcat                                                                                                                        >  1:2560315/1‑150 (MQ=255)
                            gCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCAcatca                                                                                                                      <  2:1599855/150‑1 (MQ=255)
                            gCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCAcatca                                                                                                                      <  2:2326496/150‑1 (MQ=255)
                                  tCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGAt                                                                                                                >  1:3694811/1‑150 (MQ=255)
                                   cAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATc                                                                                                               <  1:624872/150‑1 (MQ=255)
                                   cAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATc                                                                                                               >  1:3272547/1‑150 (MQ=255)
                                    aaaaTCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATcc                                                                                                              >  2:2901250/1‑150 (MQ=255)
                                     aaaTCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCt                                                                                                             >  2:2982101/1‑150 (MQ=255)
                                     aaaTCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCt                                                                                                             <  2:2160233/150‑1 (MQ=255)
                                     aaaTCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCt                                                                                                             <  1:1284043/150‑1 (MQ=255)
                                       aTCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCg                                                                                                           >  2:1551779/1‑150 (MQ=255)
                                       aTCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCg                                                                                                           <  2:2441785/150‑1 (MQ=255)
                                          aGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTc                                                                                                        >  2:3398602/1‑150 (MQ=255)
                                             cgcTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAgg                                                                                                     >  2:3300081/1‑150 (MQ=255)
                                                     cAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTg                                                                                             >  2:2484335/1‑150 (MQ=255)
                                                     cAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTg                                                                                             >  1:749551/1‑150 (MQ=255)
                                                      aGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTgg                                                                                            >  1:1317991/1‑150 (MQ=255)
                                                      aGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTgg                                                                                            <  1:3731680/150‑1 (MQ=255)
                                                        cTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGaa                                                                                          <  2:3231289/150‑1 (MQ=255)
                                                           gTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCt                                                                                       >  2:2950273/1‑150 (MQ=255)
                                                             gCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTgg                                                                                     >  1:1799378/1‑150 (MQ=255)
                                                              cAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGt                                                                                    >  2:1123217/1‑150 (MQ=255)
                                                              cAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGt                                                                                    >  1:789714/1‑150 (MQ=255)
                                                               aaaaGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGtt                                                                                   <  2:260262/150‑1 (MQ=255)
                                                                aaaGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTg                                                                                  <  1:3573926/150‑1 (MQ=255)
                                                                aaaGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTg                                                                                  >  1:95417/1‑150 (MQ=255)
                                                                aaaGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTg                                                                                  >  1:2040050/1‑150 (MQ=255)
                                                                aaaGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACCGGAATCTGGTTg                                                                                  >  1:899077/1‑150 (MQ=255)
                                                                      cGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCa                                                                            >  2:1670788/1‑150 (MQ=255)
                                                                      cGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCa                                                                            >  2:1541092/1‑150 (MQ=255)
                                                                       gTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCaa                                                                           <  1:2002812/150‑1 (MQ=255)
                                                                        tGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAAc                                                                          >  2:2285315/1‑150 (MQ=255)
                                                                                aaCTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATc                                                                  >  2:1907920/1‑150 (MQ=255)
                                                                                aaCTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATc                                                                  >  1:1451988/1‑150 (MQ=255)
                                                                                 aCTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATcc                                                                 >  1:2636853/1‑150 (MQ=255)
                                                                                 aCTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATcc                                                                 <  1:3041799/150‑1 (MQ=255)
                                                                                  cTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATccg                                                                >  2:3722674/1‑150 (MQ=255)
                                                                                     cGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATccgccg                                                             <  2:282684/150‑1 (MQ=255)
                                                                                      gatgatTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCcgccgc                                                            >  2:461869/1‑150 (MQ=255)
                                                                                        tgatTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGa                                                          >  2:625218/1‑150 (MQ=255)
                                                                                          atTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGaaa                                                        >  2:3704029/1‑150 (MQ=255)
                                                                                                tGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGcg                                                  <  2:2769296/150‑1 (MQ=255)
                                                                                                 gCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGcga                                                 <  1:2177330/150‑1 (MQ=255)
                                                                                                 gCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGcga                                                 <  1:2177316/150‑1 (MQ=255)
                                                                                                  ccGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGcgag                                                <  1:3538137/150‑1 (MQ=255)
                                                                                                    gggTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGcgagcc                                              >  2:796801/1‑150 (MQ=255)
                                                                                                        gACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGcgagccgagc                                          >  1:1029937/1‑150 (MQ=255)
                                                                                                         aCAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCt                                         <  1:1907920/150‑1 (MQ=255)
                                                                                                          cAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCta                                        >  1:640398/1‑150 (MQ=255)
                                                                                                           aGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCtat                                       >  1:3240027/1‑150 (MQ=255)
                                                                                                           aGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCtat                                       <  1:2933823/150‑1 (MQ=255)
                                                                                                             tAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATaa                                     <  2:749551/150‑1 (MQ=255)
                                                                                                              aaaGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAAc                                    <  2:2404499/150‑1 (MQ=255)
                                                                                                              aaaGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAAc                                    >  1:3081797/1‑150 (MQ=255)
                                                                                                                 gCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTg                                 >  2:2037232/1‑150 (MQ=255)
                                                                                                                  cagtCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGGGTGCCACATCATTTGAGCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGt                                <  2:3693555/147‑1 (MQ=255)
                                                                                                                  cATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGt                                >  2:556665/1‑150 (MQ=255)
                                                                                                                  cATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGt                                <  1:1442433/150‑1 (MQ=255)
                                                                                                                  cATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGt                                >  2:2145383/1‑150 (MQ=255)
                                                                                                                  cATTCCTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGt                                >  2:1620850/1‑150 (MQ=255)
                                                                                                                      ccTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTgg                            <  1:1200601/150‑1 (MQ=255)
                                                                                                                      ccTCGCGCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTGGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTgg                            >  2:3533822/1‑150 (MQ=255)
                                                                                                                         cgcgCAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGgtgt                         <  2:2105390/150‑1 (MQ=255)
                                                                                                                           cgcAACTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCa                       >  2:135085/1‑150 (MQ=255)
                                                                                                                              aaCTCTCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAg                    >  1:58086/1‑150 (MQ=255)
                                                                                                                              aaCTATCGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAg                    >  2:2181660/1‑150 (MQ=255)
                                                                                                                                 tctcGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCct                 <  1:2982101/150‑1 (MQ=255)
                                                                                                                                 tctcGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCct                 <  1:1024221/150‑1 (MQ=255)
                                                                                                                                  ctcGCTGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCctc                >  2:1035594/1‑150 (MQ=255)
                                                                                                                                       tGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCa           <  1:3463436/150‑1 (MQ=255)
                                                                                                                                       tGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCa           >  1:548577/1‑150 (MQ=255)
                                                                                                                                       tGCCCGCCCAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCa           >  1:1652481/1‑150 (MQ=255)
                                                                                                                                        gcccgcccAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCaa          <  1:1396772/150‑1 (MQ=255)
                                                                                                                                         cccgcccAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAAc         >  1:2763874/1‑150 (MQ=255)
                                                                                                                                              ccAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGaa    <  1:39856/150‑1 (MQ=255)
                                                                                                                                               cAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGaaa   <  1:2292009/150‑1 (MQ=255)
                                                                                                                                               cAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGaaa   <  1:1573502/150‑1 (MQ=255)
                                                                                                                                               cAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGaaa   <  1:709642/150‑1 (MQ=255)
                                                                                                                                               cAACAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGaaa   <  1:709658/150‑1 (MQ=255)
                                                                                                                                                aaCAGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAg  >  1:3782549/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCCTCGCGCAACTCTCGCTGCCCGCCCAACTGGCAAGCCAGCAAAGCGGTGTGCCACATCATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAG  >  CP009273/1133164‑1133457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑