Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,156,310 G→T S233* (TCA→TAA)  fhuE ← ferric‑rhodotorulic acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,156,3100GT99.6% 787.8 / NA 243S233* (TCA→TAA) fhuEferric‑rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (108/134);  total (109/134)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

TCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGC  >  CP009273/1156164‑1156455
                                                                                                                                                  |                                                                                                                                                 
tCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGt                                                                                                                                                <  2:1747068/150‑1 (MQ=255)
tCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGt                                                                                                                                                <  1:867607/150‑1 (MQ=255)
 ccAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTc                                                                                                                                               >  1:3274214/1‑150 (MQ=255)
   aGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGt                                                                                                                                             >  1:2990226/1‑150 (MQ=255)
   aGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGt                                                                                                                                             >  1:214249/1‑150 (MQ=255)
   aGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGt                                                                                                                                             >  1:1656947/1‑150 (MQ=255)
    gcgcGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGtt                                                                                                                                            <  1:695413/150‑1 (MQ=255)
     cgcgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTa                                                                                                                                           <  1:1363889/150‑1 (MQ=255)
        ggTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTc                                                                                                                                        >  2:2966908/1‑150 (MQ=255)
         gTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCt                                                                                                                                       <  1:1160897/150‑1 (MQ=255)
          tAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTg                                                                                                                                      <  2:3197847/150‑1 (MQ=255)
          tAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTg                                                                                                                                      <  2:3068731/150‑1 (MQ=255)
           aaaCCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTgg                                                                                                                                     <  2:1176601/150‑1 (MQ=255)
           aaaCCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTgg                                                                                                                                     >  2:1585365/1‑150 (MQ=255)
            aaCCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGt                                                                                                                                    <  1:2514065/150‑1 (MQ=255)
              ccGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAg                                                                                                                                  <  2:3380565/150‑1 (MQ=255)
                gCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgcc                                                                                                                                <  2:2242915/150‑1 (MQ=255)
                 ccccAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccg                                                                                                                               <  2:2998029/150‑1 (MQ=255)
                 ccccAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccg                                                                                                                               <  2:3287414/150‑1 (MQ=255)
                     aGGTAGGGCTATTAACATCAATGCGCTGATATTCGTCACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGa                                                                                                                           >  2:1024339/1‑150 (MQ=255)
                      ggTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGAc                                                                                                                          <  1:3059845/150‑1 (MQ=255)
                          tggCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAAtt                                                                                                                      >  2:2716365/2‑150 (MQ=255)
                             cTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgc                                                                                                                   >  2:2586092/1‑150 (MQ=255)
                               aTTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcgc                                                                                                                 >  1:1162748/1‑150 (MQ=255)
                                ttAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcgcg                                                                                                                <  2:792280/150‑1 (MQ=255)
                                  aaCATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcgcgcg                                                                                                              >  1:1461336/1‑150 (MQ=255)
                                   aCATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCgg                                                                                                             >  1:3469903/1‑150 (MQ=255)
                                    cATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGa                                                                                                            <  2:1199198/150‑1 (MQ=255)
                                      tCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGAtt                                                                                                          <  1:2401364/150‑1 (MQ=255)
                                        aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGAtttt                                                                                                        >  1:1735046/1‑150 (MQ=255)
                                           gcgcTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTAcc                                                                                                     <  2:2727701/150‑1 (MQ=255)
                                           gcgcTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTAcc                                                                                                     <  1:1321599/150‑1 (MQ=255)
                                               tGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCt                                                                                                 <  1:1048518/150‑1 (MQ=255)
                                               tGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCt                                                                                                 <  1:3683091/150‑1 (MQ=255)
                                               tGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCt                                                                                                 >  1:2276396/1‑150 (MQ=255)
                                                gATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCtt                                                                                                <  1:3283420/150‑1 (MQ=255)
                                                   atTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCgg                                                                                             >  2:3100913/1‑150 (MQ=255)
                                                   atTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCgg                                                                                             >  2:3474610/1‑150 (MQ=255)
                                                    ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGt                                                                                            <  1:260780/150‑1 (MQ=255)
                                                      cGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGa                                                                                          <  1:2107315/150‑1 (MQ=255)
                                                                tGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTg                                                                                >  2:2167970/1‑150 (MQ=255)
                                                                tGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTg                                                                                >  2:1331628/1‑150 (MQ=255)
                                                                 gAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACACTTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGt                                                                               >  1:3776054/1‑150 (MQ=255)
                                                                    aGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCGTCGGTGAGTGGGCTTTGTaaa                                                                            >  2:2937594/1‑150 (MQ=255)
                                                                     gCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAAt                                                                           <  1:141571/150‑1 (MQ=255)
                                                                      tgtcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATc                                                                          >  2:188823/2‑150 (MQ=255)
                                                                      cgtcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATc                                                                          >  2:397125/1‑150 (MQ=255)
                                                                        tcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCg                                                                        <  1:2849711/150‑1 (MQ=255)
                                                                           tAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCca                                                                     <  2:3469903/150‑1 (MQ=255)
                                                                           tAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCca                                                                     >  2:3753767/1‑150 (MQ=255)
                                                                            aaGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGATAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCcac                                                                    >  1:3592881/1‑150 (MQ=255)
                                                                              gATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACAt                                                                  <  1:419206/150‑1 (MQ=255)
                                                                               aTCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATa                                                                 <  2:2793783/150‑1 (MQ=255)
                                                                                tCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATAc                                                                <  1:2735726/150‑1 (MQ=255)
                                                                                    cTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGtt                                                            >  1:3201803/1‑150 (MQ=255)
                                                                                        aTCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCttt                                                        >  1:48731/1‑150 (MQ=255)
                                                                                        aTCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCttt                                                        <  1:1085800/150‑1 (MQ=255)
                                                                                        aTCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCttt                                                        <  1:986154/150‑1 (MQ=255)
                                                                                             cATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTcc                                                   >  1:2714873/1‑150 (MQ=255)
                                                                                              aTCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCa                                                  <  2:1257759/150‑1 (MQ=255)
                                                                                              aTCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCa                                                  >  1:1947340/1‑150 (MQ=255)
                                                                                              aTCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCa                                                  <  1:2933369/150‑1 (MQ=255)
                                                                                              aTCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCa                                                  <  1:36367/150‑1 (MQ=255)
                                                                                              aTCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCa                                                  <  2:1918777/150‑1 (MQ=255)
                                                                                                cGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGc                                                <  1:3131987/150‑1 (MQ=255)
                                                                                                     aTGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCg                                           <  1:3434116/150‑1 (MQ=255)
                                                                                                      tGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGt                                          >  1:178523/1‑150 (MQ=255)
                                                                                                          cGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTc                                      >  2:2530687/1‑150 (MQ=255)
                                                                                                           gagaAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTcc                                     <  2:596045/150‑1 (MQ=255)
                                                                                                            agaagaAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccg                                    <  1:3089729/150‑1 (MQ=255)
                                                                                                              aagaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgcc                                  <  2:170600/150‑1 (MQ=255)
                                                                                                              aagaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgcc                                  >  2:3518859/1‑150 (MQ=255)
                                                                                                              aagaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgcc                                  <  2:3645982/150‑1 (MQ=255)
                                                                                                                  agGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCgaga                              <  1:2754591/150‑1 (MQ=255)
                                                                                                                   ggTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGAc                             >  1:2908743/1‑150 (MQ=255)
                                                                                                                   ggTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGAc                             >  2:3324869/1‑150 (MQ=255)
                                                                                                                     tCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACAt                           <  2:2133031/150‑1 (MQ=255)
                                                                                                                      cTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATc                          >  1:1550484/1‑150 (MQ=255)
                                                                                                                      cTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATc                          >  1:946325/1‑150 (MQ=255)
                                                                                                                      cTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATc                          <  2:2123509/150‑1 (MQ=255)
                                                                                                                      cTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATc                          <  1:1259452/150‑1 (MQ=255)
                                                                                                                           cACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCtt                     >  1:1287097/1‑150 (MQ=255)
                                                                                                                             cTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTa                   >  1:1908825/1‑150 (MQ=255)
                                                                                                                               gttgtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTaaa                 >  1:1001457/1‑150 (MQ=255)
                                                                                                                                ttgtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAAt                <  2:1461336/150‑1 (MQ=255)
                                                                                                                                  gtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTc              >  2:2413472/1‑150 (MQ=255)
                                                                                                                                    aGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCTGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCAc            >  1:2317231/1‑150 (MQ=255)
                                                                                                                                    aGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCAc            >  2:1982586/1‑150 (MQ=255)
                                                                                                                                    aGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCAc            <  1:3573583/150‑1 (MQ=255)
                                                                                                                                    aGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCAc            >  2:474656/1‑150 (MQ=255)
                                                                                                                                    aGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTATAAATTCAc            >  2:844411/1‑150 (MQ=255)
                                                                                                                                     gCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACg           >  2:2553658/1‑150 (MQ=255)
                                                                                                                                     gCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACg           <  1:1675685/150‑1 (MQ=255)
                                                                                                                                       ggTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGAc         >  1:2238518/1‑150 (MQ=255)
                                                                                                                                       ggTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGAc         >  2:3632989/1‑150 (MQ=255)
                                                                                                                                       ggTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGAc         >  1:1472281/1‑150 (MQ=255)
                                                                                                                                        gTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACt        <  2:301928/150‑1 (MQ=255)
                                                                                                                                            aGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTc    >  2:665581/1‑150 (MQ=255)
                                                                                                                                             gCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcg   <  2:1294796/150‑1 (MQ=255)
                                                                                                                                              ccATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTcgc  >  2:874463/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCCAGCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGC  >  CP009273/1156164‑1156455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑