Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,281,042 C→A R150S (CGT→AGT)  narJ → molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,281,0420CA100.0% 758.6 / NA 227R150S (CGT→AGT) narJmolybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (119/108);  total (119/108)

Only 100 of 227 total aligned reads displayed.

CACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGCGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGGAAGAAGAGCAG  >  CP009273/1280895‑1281188
                                                                                                                                                   |                                                                                                                                                  
caCGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGt                                                                                                                                                  >  2:584639/1‑150 (MQ=255)
 aCGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTg                                                                                                                                                 <  2:1023617/150‑1 (MQ=255)
   ggCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGaa                                                                                                                                               >  1:73758/1‑150 (MQ=255)
   ggCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGaa                                                                                                                                               <  1:2508620/150‑1 (MQ=255)
   ggCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGaa                                                                                                                                               <  1:1462456/150‑1 (MQ=255)
       tGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGcc                                                                                                                                           >  1:3174747/1‑150 (MQ=255)
       tGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGcc                                                                                                                                           <  2:3521405/150‑1 (MQ=255)
         cAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCACAGCGAAGCCGTGGAAGGTTTGAAAGAGATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGt                                                                                                                                         <  2:2659652/150‑1 (MQ=255)
            ttAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTAt                                                                                                                                      <  2:1351339/150‑1 (MQ=255)
             tAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATg                                                                                                                                     >  1:873910/1‑150 (MQ=255)
              aaaCAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGc                                                                                                                                    >  1:1474602/1‑150 (MQ=255)
                 cAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGt                                                                                                                                 >  1:1299821/1‑150 (MQ=255)
                 cAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGt                                                                                                                                 <  2:128664/150‑1 (MQ=255)
                    ccGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCt                                                                                                                              >  2:399915/1‑150 (MQ=255)
                    ccGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCt                                                                                                                              <  2:2297763/150‑1 (MQ=255)
                     cgcgAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTg                                                                                                                             >  1:3295442/1‑150 (MQ=255)
                      gcgAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGt                                                                                                                            <  2:801257/150‑1 (MQ=255)
                        gAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGttt                                                                                                                          <  1:2683678/150‑1 (MQ=255)
                        gAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGttt                                                                                                                          <  2:487402/150‑1 (MQ=255)
                         aaTTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTg                                                                                                                         <  1:70605/150‑1 (MQ=255)
                            tGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATc                                                                                                                      <  2:1734876/150‑1 (MQ=255)
                            tGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATc                                                                                                                      >  1:567363/1‑150 (MQ=255)
                            tGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATc                                                                                                                      >  1:2724366/1‑150 (MQ=255)
                               cGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATctgc                                                                                                                   >  2:1674795/1‑150 (MQ=255)
                               cGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATctgc                                                                                                                   >  1:1621815/1‑150 (MQ=255)
                                ggACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATctgct                                                                                                                  <  2:2423856/150‑1 (MQ=255)
                                 gACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATctgctg                                                                                                                 >  1:1114269/1‑150 (MQ=255)
                                 gACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATctgctg                                                                                                                 >  1:1114456/1‑150 (MQ=255)
                                          ccGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTg                                                                                                        <  2:2749519/150‑1 (MQ=255)
                                            gCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGc                                                                                                      <  2:1541435/150‑1 (MQ=255)
                                              tGTATCTGGAGGACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGa                                                                                                    <  1:3747078/150‑1 (MQ=255)
                                                tATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAAt                                                                                                  >  2:1949331/1‑150 (MQ=255)
                                                  tCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATAc                                                                                                >  2:3451217/1‑150 (MQ=255)
                                                   cTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATAcc                                                                                               <  1:64206/150‑1 (MQ=255)
                                                      gAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCt                                                                                            <  1:2032908/150‑1 (MQ=255)
                                                       aGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTa                                                                                           >  2:1994344/1‑150 (MQ=255)
                                                        gTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTAt                                                                                          <  1:1160035/150‑1 (MQ=255)
                                                           ccTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGa                                                                                       >  2:3164944/1‑150 (MQ=255)
                                                            cTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGAc                                                                                      >  2:3436346/1‑150 (MQ=255)
                                                              ggCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAg                                                                                    >  1:1040295/1‑150 (MQ=255)
                                                               gcgcAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGc                                                                                   <  2:343947/150‑1 (MQ=255)
                                                                cgcAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCg                                                                                  >  2:72481/1‑150 (MQ=255)
                                                                   aGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACa                                                                               >  2:2692340/1‑150 (MQ=255)
                                                                    gCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACaa                                                                              <  1:3796088/150‑1 (MQ=255)
                                                                       gCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGt                                                                           <  2:3618680/150‑1 (MQ=255)
                                                                         cGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTgg                                                                         >  2:762989/1‑150 (MQ=255)
                                                                          gCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGc                                                                        <  1:1377323/150‑1 (MQ=255)
                                                                           cAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCg                                                                       >  2:3036931/1‑150 (MQ=255)
                                                                           cAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCg                                                                       >  2:1449641/1‑150 (MQ=255)
                                                                            aaaGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCgg                                                                      <  1:1826134/150‑1 (MQ=255)
                                                                             aaGCGAAGCCGTGGAAGGTTTGAAAGATATCTCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGa                                                                     >  1:2192036/1‑150 (MQ=255)
                                                                             aaGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGa                                                                     <  1:3106647/150‑1 (MQ=255)
                                                                             aaGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGa                                                                     >  2:511704/1‑150 (MQ=255)
                                                                                cGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGaaaa                                                                  <  1:1688828/150‑1 (MQ=255)
                                                                                 gAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGaaaaa                                                                 >  1:2221590/1‑150 (MQ=255)
                                                                                 gAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGaaaaa                                                                 >  1:563753/1‑150 (MQ=255)
                                                                                 gAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGaaaaa                                                                 >  1:1740345/1‑150 (MQ=255)
                                                                                  aaGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGaaaaaa                                                                >  2:2361939/1‑150 (MQ=255)
                                                                                   aGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAAt                                                               >  1:3026127/1‑150 (MQ=255)
                                                                                      cGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGc                                                            >  2:3116862/1‑150 (MQ=255)
                                                                                      cGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGc                                                            >  1:2991254/1‑150 (MQ=255)
                                                                                         ggAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCcga                                                         <  2:2650103/150‑1 (MQ=255)
                                                                                          gAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCcgac                                                        <  2:1434129/150‑1 (MQ=255)
                                                                                           aaGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCcgacg                                                       <  1:199122/150‑1 (MQ=255)
                                                                                             ggTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGaa                                                     <  1:2133653/150‑1 (MQ=255)
                                                                                                 tGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGACgcgc                                                 >  2:2493065/1‑150 (MQ=255)
                                                                                                  gAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAgcgcg                                                <  1:2355806/150‑1 (MQ=255)
                                                                                                  gAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAgcgcg                                                >  1:192920/1‑150 (MQ=255)
                                                                                                  gAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAgcgcg                                                >  1:2102471/1‑150 (MQ=255)
                                                                                                   aaaGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAgcgcgc                                               <  2:2627362/150‑1 (MQ=255)
                                                                                                     aGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCga                                             >  1:400267/1‑150 (MQ=255)
                                                                                                       atatCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCgatg                                           >  1:2393158/1‑150 (MQ=255)
                                                                                                       atatCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCgatg                                           >  2:1612039/1‑150 (MQ=255)
                                                                                                       atatCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCgatg                                           <  2:3507800/150‑1 (MQ=255)
                                                                                                       atatCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCgatg                                           <  2:2936853/150‑1 (MQ=255)
                                                                                                               ccGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATAcgccg                                   <  1:3154105/150‑1 (MQ=255)
                                                                                                                cGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATAcgccgc                                  >  1:91096/1‑150 (MQ=255)
                                                                                                                 gATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCa                                 >  2:2541727/1‑150 (MQ=255)
                                                                                                                  aTTCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAg                                >  1:2454793/1‑150 (MQ=255)
                                                                                                                   ttCTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAgg                               <  1:504038/150‑1 (MQ=255)
                                                                                                                     cTGGCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGACGCGCGCGATGATACGCCGCAGgcg                             >  1:2760231/1‑150 (MQ=255)
                                                                                                                        gCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTg                          <  1:61480/150‑1 (MQ=255)
                                                                                                                        gCATTGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTg                          <  2:2192036/150‑1 (MQ=255)
                                                                                                                            tGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATg                      <  2:1114456/150‑1 (MQ=255)
                                                                                                                            tGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATg                      <  2:3313557/150‑1 (MQ=255)
                                                                                                                            tGCTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATg                      <  2:1114269/150‑1 (MQ=255)
                                                                                                                              cTGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCt                    <  1:3436314/150‑1 (MQ=255)
                                                                                                                               tGAGCGCGCGTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTg                   >  2:3560519/1‑150 (MQ=255)
                                                                                                                                  gcgcgcGTCTGCAACTGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCt                <  1:3529671/150‑1 (MQ=255)
                                                                                                                                     cgcgTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTggg             >  2:3622092/1‑150 (MQ=255)
                                                                                                                                       cgTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGACGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGgaa           >  2:1884330/1‑150 (MQ=255)
                                                                                                                                       cgTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGgaa           >  2:1727037/1‑150 (MQ=255)
                                                                                                                                        gTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGgaag          <  2:286494/150‑1 (MQ=255)
                                                                                                                                        gTCTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGgaag          <  1:397641/150‑1 (MQ=255)
                                                                                                                                          cTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGgaagaa        <  1:853356/150‑1 (MQ=255)
                                                                                                                                          cTGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGgaagaa        >  1:3475071/1‑150 (MQ=255)
                                                                                                                                           tGCAACAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGGaagaag       <  1:3063905/150‑1 (MQ=255)
                                                                                                                                              aaCAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGGAAGAAGAGc    >  2:3429661/1‑150 (MQ=255)
                                                                                                                                               aCAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGGAAGAAGAGCa   <  1:813705/150‑1 (MQ=255)
                                                                                                                                                cAGAGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGGAAGAAGAGCAg  >  2:3726956/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTGCAACAGCGTGAAAGCCGTTATGCCGTGCTGTTTGATCTGCTGCTGAAACTGGCGAATACCGCTATCGACAGCGACAAAGTGGCGGAAAAAATTGCCGACGAAGCGCGCGATGATACGCCGCAGGCGCTGGATGCTGTCTGGGAAGAAGAGCAG  >  CP009273/1280895‑1281188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑