BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,331,654	G→T	D523Y (GAT→TAT)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,331,654	0	G	T	100.0%	763.4 / NA	228	D523Y (GAT→TAT)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (131/97); total (131/97)

Only 100 of 228 total aligned reads displayed.

AAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAG  >  CP009273/1331507‑1331800

aaaGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTAt                                                                                                                                                  <  2:1865926/150‑1 (MQ=255)

  aGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTAttt                                                                                                                                                <  2:2456498/150‑1 (MQ=255)

  aGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTAttt                                                                                                                                                >  2:1645189/1‑150 (MQ=255)

   gTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTa                                                                                                                                               <  1:1178666/150‑1 (MQ=255)

      gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAAcc                                                                                                                                            >  1:2611856/1‑150 (MQ=255)

        ttCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGt                                                                                                                                          >  2:2683102/1‑150 (MQ=255)

          cTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTcg                                                                                                                                        >  1:13672/1‑150 (MQ=255)

            gATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTcggt                                                                                                                                      >  1:1614131/1‑150 (MQ=255)

             aTTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTcggtg                                                                                                                                     <  2:1480308/150‑1 (MQ=255)

              ttATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTcggtgc                                                                                                                                    <  2:2001692/150‑1 (MQ=255)

               tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTcggtgcg                                                                                                                                   <  1:2405616/150‑1 (MQ=255)

               tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTcggtgcg                                                                                                                                   >  1:3475196/1‑150 (MQ=255)

                 tCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTcggtgcggt                                                                                                                                 >  1:2509497/1‑150 (MQ=255)

                     gCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTg                                                                                                                             <  1:2701331/150‑1 (MQ=255)

                      cAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGt                                                                                                                            <  1:1521707/150‑1 (MQ=255)

                        aaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTcc                                                                                                                          >  1:1024731/1‑150 (MQ=255)

                          aGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCgg                                                                                                                        <  1:1783362/150‑1 (MQ=255)

                          aGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCgg                                                                                                                        <  1:2192726/150‑1 (MQ=255)

                            cGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCa                                                                                                                      >  2:3171905/1‑150 (MQ=255)

                             gAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCaa                                                                                                                     >  2:1514113/1‑150 (MQ=255)

                             gAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCaa                                                                                                                     >  2:277899/1‑150 (MQ=255)

                              aaaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAAc                                                                                                                    >  2:806873/1‑150 (MQ=255)

                               aaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAAcc                                                                                                                   >  2:3440252/1‑150 (MQ=255)

                                aCTGACACCGTATCTCGACGAACTGGGGTTTAACATTGTGGGATACGGTTGTACCACCTGAATTGGTAACTCTGGGCCGCTGCCCGAACCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGATGCGGTGCTGTCCGGCAACCg                                                                                                                  >  1:3125294/1‑150 (MQ=255)

                                 cTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGt                                                                                                                 >  2:296528/1‑150 (MQ=255)

                                  tGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTa                                                                                                                >  2:2913511/1‑150 (MQ=255)

                                  tGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTa                                                                                                                >  1:3069090/1‑150 (MQ=255)

                                   gACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTaa                                                                                                               <  1:1008894/150‑1 (MQ=255)

                                      acCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACtt                                                                                                            >  1:3142260/1‑150 (MQ=255)

                                      acCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACtt                                                                                                            >  1:1445240/1‑150 (MQ=255)

                                        cGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTg                                                                                                          >  2:3112972/1‑150 (MQ=255)

                                           aTCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAg                                                                                                       >  2:3534935/1‑150 (MQ=255)

                                           aTCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAg                                                                                                       <  1:568308/150‑1 (MQ=255)

                                             ctcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGc                                                                                                     >  1:2451739/1‑150 (MQ=255)

                                               cgacgaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCg                                                                                                   >  2:1320135/1‑150 (MQ=255)

                                                 acgaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTa                                                                                                 >  2:258129/1‑150 (MQ=255)

                                                    aaCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcc                                                                                              >  1:2386698/1‑150 (MQ=255)

                                                     aCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGAAACCGTAACTTTGAAGGCCGTatcca                                                                                             >  2:3411647/1‑150 (MQ=255)

                                                      cTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccat                                                                                            >  2:1985463/1‑150 (MQ=255)

                                                         gggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCg                                                                                         >  2:497606/1‑150 (MQ=255)

                                                          ggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGc                                                                                        >  1:1922388/1‑150 (MQ=255)

                                                            tttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTg                                                                                      <  1:3715030/150‑1 (MQ=255)

                                                             ttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTgg                                                                                     >  1:1572140/1‑150 (MQ=255)

                                                             ttAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTgg                                                                                     <  1:1680763/150‑1 (MQ=255)

                                                               aaCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGtt                                                                                   <  1:1147940/150‑1 (MQ=255)

                                                               aaCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGtt                                                                                   >  2:828147/1‑150 (MQ=255)

                                                                  cTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaa                                                                                <  2:2199965/150‑1 (MQ=255)

                                                                      tgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaacta                                                                            >  2:1237538/1‑150 (MQ=255)

                                                                       gggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaa                                                                           >  2:470190/1‑150 (MQ=255)

                                                                       gggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaa                                                                           >  2:469774/1‑150 (MQ=255)

                                                                        ggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaac                                                                          >  1:2366965/1‑150 (MQ=255)

                                                                         gATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaact                                                                         <  1:1803480/150‑1 (MQ=255)

                                                                          aTACGGTTGTACAACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg                                                                        <  2:1126711/150‑1 (MQ=255)

                                                                              ggTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctg                                                                    <  1:3007233/150‑1 (MQ=255)

                                                                              ggTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctg                                                                    >  1:420511/1‑150 (MQ=255)

                                                                              ggTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCAGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctg                                                                    >  1:906293/1‑150 (MQ=255)

                                                                               gTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctgg                                                                   >  1:2880964/1‑150 (MQ=255)

                                                                                ttGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACtggctggc                                                                  >  2:609172/1‑150 (MQ=255)

                                                                                   tACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTc                                                               <  2:784844/150‑1 (MQ=255)

                                                                                     ccaccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgc                                                             >  1:3498925/1‑150 (MQ=255)

                                                                                      caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgcc                                                            <  2:1801441/150‑1 (MQ=255)

                                                                                      caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgcc                                                            >  2:2056537/1‑150 (MQ=255)

                                                                                      caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgcc                                                            <  2:3800102/150‑1 (MQ=255)

                                                                                                 gTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtgg                                                 >  2:2757828/1‑150 (MQ=255)

                                                                                                  tAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTggtggt                                                <  2:1693988/150‑1 (MQ=255)

                                                                                                   aaCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGtt                                               <  2:3496670/150‑1 (MQ=255)

                                                                                                    aCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTg                                              <  1:1398368/150‑1 (MQ=255)

                                                                                                    aCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTg                                              >  2:919512/1‑150 (MQ=255)

                                                                                                        tGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTa                                          <  1:448915/150‑1 (MQ=255)

                                                                                                         gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTAt                                         <  1:806873/150‑1 (MQ=255)

                                                                                                          ggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATg                                        <  1:3219704/150‑1 (MQ=255)

                                                                                                            ccGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATgcg                                      <  2:1840160/150‑1 (MQ=255)

                                                                                                             cGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATgcgc                                     >  1:965352/1‑150 (MQ=255)

                                                                                                               cTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTg                                   <  1:2019215/150‑1 (MQ=255)

                                                                                                               cTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTg                                   <  1:2754054/150‑1 (MQ=255)

                                                                                                                tGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTgg                                  <  1:1667536/150‑1 (MQ=255)

                                                                                                                  cccGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCg                                >  2:285444/1‑150 (MQ=255)

                                                                                                                    cGATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCggg                              >  1:3663408/1‑150 (MQ=255)

                                                                                                                     gATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                             >  1:207974/1‑150 (MQ=255)

                                                                                                                     gATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                             >  2:2665370/1‑150 (MQ=255)

                                                                                                                     gATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                             >  1:3274918/1‑150 (MQ=255)

                                                                                                                     gATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                             >  2:656902/1‑150 (MQ=255)

                                                                                                                     gATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                             >  1:761743/1‑150 (MQ=255)

                                                                                                                        ccTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAat                          >  1:989519/1‑150 (MQ=255)

                                                                                                                         cTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAata                         <  1:418441/150‑1 (MQ=255)

                                                                                                                           aTCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATg                       <  1:3382359/150‑1 (MQ=255)

                                                                                                                                aaCGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAatat                  <  2:752819/150‑1 (MQ=255)

                                                                                                                                  cGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCa                <  2:2565517/150‑1 (MQ=255)

                                                                                                                                   ggCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCaa               >  1:1282045/1‑150 (MQ=255)

                                                                                                                                    gCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAAc              <  2:3073781/150‑1 (MQ=255)

                                                                                                                                      aaTCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCt            <  2:16197/150‑1 (MQ=255)

                                                                                                                                      aaTCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCt            <  1:258083/150‑1 (MQ=255)

                                                                                                                                         cAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGc         >  2:970012/1‑150 (MQ=255)

                                                                                                                                          aaaaaaGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCt        >  2:659646/1‑150 (MQ=255)

                                                                                                                                           aaaaaGGCTATTTAACCGTCGGTGCGGTGCTGTCCAGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCtt       <  2:906293/150‑1 (MQ=255)

                                                                                                                                            aaaaGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTc      >  2:3371699/1‑150 (MQ=255)

                                                                                                                                            aaaaGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTc      >  2:1431394/1‑150 (MQ=255)

                                                                                                                                             aaaGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCt     >  1:611997/1‑150 (MQ=255)

                                                                                                                                                ggCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAg  >  2:1445412/1‑150 (MQ=255)

                                                                                                                                                ggCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAg  >  1:815594/1‑150 (MQ=255)

AAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAG  >  CP009273/1331507‑1331800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`