Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,335,356 A→T H181L (CAT→CTT)  yciM → envelope integrity maintenance protein; EnvC‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,335,3560AT100.0% 693.3 / NA 207H181L (CAT→CTT) yciMenvelope integrity maintenance protein; EnvC‑interacting protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (108/99);  total (108/99)

Only 100 of 210 total aligned reads displayed.

AGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAT  >  CP009273/1335209‑1335502
                                                                                                                                                   |                                                                                                                                                   
aGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCttt                                                                                                                                                   >  2:1683157/1‑150 (MQ=255)
   tGACAGATGAAAATGACATCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGAAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTGTTCt                                                                                                                                                >  2:2842773/1‑150 (MQ=255)
    gACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTa                                                                                                                                               <  1:2210058/150‑1 (MQ=255)
      ccGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACt                                                                                                                                             >  2:152879/1‑150 (MQ=255)
      ccGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACt                                                                                                                                             >  2:2062520/1‑150 (MQ=255)
       cGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACtg                                                                                                                                            >  1:1102570/1‑150 (MQ=255)
       cGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACtg                                                                                                                                            >  2:3741496/1‑150 (MQ=255)
          tGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGa                                                                                                                                         <  1:914901/150‑1 (MQ=255)
          tGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGa                                                                                                                                         <  1:3358467/150‑1 (MQ=255)
           gAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAg                                                                                                                                        >  1:3675804/1‑150 (MQ=255)
              aCTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTa                                                                                                                                     <  1:521437/150‑1 (MQ=255)
                tGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGc                                                                                                                                   <  1:3185972/150‑1 (MQ=255)
                 gACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGcc                                                                                                                                  >  1:387321/1‑150 (MQ=255)
                        gCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTgcagc                                                                                                                           >  1:2843210/1‑150 (MQ=255)
                               cgcgcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGc                                                                                                                    <  1:3302023/150‑1 (MQ=255)
                                  gcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAg                                                                                                                 >  1:3571318/1‑150 (MQ=255)
                                  gcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAg                                                                                                                 >  1:2671771/1‑150 (MQ=255)
                                      cAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGcgac                                                                                                             <  2:3703935/150‑1 (MQ=255)
                                      cAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGcgac                                                                                                             <  1:2385978/150‑1 (MQ=255)
                                          aGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtc                                                                                                         >  2:1216333/1‑150 (MQ=255)
                                          aGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtc                                                                                                         >  1:25512/1‑150 (MQ=255)
                                           gTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtct                                                                                                        <  1:1226159/150‑1 (MQ=255)
                                           gTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtct                                                                                                        >  2:3300679/1‑150 (MQ=255)
                                            ttGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtctc                                                                                                       <  2:3460846/150‑1 (MQ=255)
                                                tACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATc                                                                                                   <  1:1236868/150‑1 (MQ=255)
                                                  cAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGt                                                                                                 >  2:158684/1‑150 (MQ=255)
                                                  cAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGt                                                                                                 >  2:1710926/1‑150 (MQ=255)
                                                   aaaTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTg                                                                                                >  2:1443444/1‑150 (MQ=255)
                                                     aTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGcc                                                                                              <  1:3549779/150‑1 (MQ=255)
                                                     aTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGcc                                                                                              <  2:3264581/150‑1 (MQ=255)
                                                          ccAGGCTACTAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAc                                                                                         <  1:1912952/150‑1 (MQ=255)
                                                          ccAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAc                                                                                         <  1:1838091/150‑1 (MQ=255)
                                                           cAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAcc                                                                                        <  2:677772/150‑1 (MQ=255)
                                                            aGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCt                                                                                       >  1:1358425/1‑150 (MQ=255)
                                                             ggCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCtt                                                                                      <  2:2889434/150‑1 (MQ=255)
                                                                  ccAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑aa                                                                                >  2:2440455/1‑150 (MQ=255)
                                                                    agtgagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑aaaa                                                                              >  1:760540/1‑150 (MQ=255)
                                                                       gagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑aaaaaaa                                                                           >  2:297663/1‑150 (MQ=255)
                                                                        agtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAg                                                                          >  2:2571517/1‑150 (MQ=255)
                                                                              ataaaGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGggcgg                                                                    >  1:2485252/3‑150 (MQ=255)
                                                                               gAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGggcggc                                                                   <  1:568990/150‑1 (MQ=255)
                                                                                aaaGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGggcggcg                                                                  >  1:1989476/1‑150 (MQ=255)
                                                                                   gCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCa                                                               <  1:1471547/150‑1 (MQ=255)
                                                                                    cAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAg                                                              <  1:1041345/150‑1 (MQ=255)
                                                                                    cAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAg                                                              <  1:633446/150‑1 (MQ=255)
                                                                                    cAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAg                                                              <  1:633538/150‑1 (MQ=255)
                                                                                     aaTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGa                                                             >  1:3163678/1‑150 (MQ=255)
                                                                                     aaTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGa                                                             >  2:2259532/1‑150 (MQ=255)
                                                                                      aTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGAt                                                            >  2:3046663/1‑150 (MQ=255)
                                                                                      aTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGAt                                                            >  2:871129/1‑150 (MQ=255)
                                                                                        tGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATaa                                                          >  2:80872/1‑150 (MQ=255)
                                                                                          aTGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATaaaa                                                        >  2:3403406/1‑150 (MQ=255)
                                                                                          aTGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATaaaa                                                        >  2:2702103/1‑150 (MQ=255)
                                                                                              tGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAg                                                    >  2:3614646/1‑150 (MQ=255)
                                                                                              tGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAg                                                    >  2:1719972/1‑150 (MQ=255)
                                                                                               gCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAgc                                                   <  1:1340419/150‑1 (MQ=255)
                                                                                                 cGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAgcgc                                                 <  1:2247154/150‑1 (MQ=255)
                                                                                                  gAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑‑AAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                               <  1:2360243/150‑1 (MQ=255)
                                                                                                   aaCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                               >  2:2620431/1‑150 (MQ=255)
                                                                                                   aaCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                               >  2:532975/1‑150 (MQ=255)
                                                                                                   aaCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                               >  2:218849/1‑150 (MQ=255)
                                                                                                    aCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCcg                                              <  2:1493527/150‑1 (MQ=255)
                                                                                                    aCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCcg                                              >  1:2873201/1‑150 (MQ=255)
                                                                                                        ctagTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTa                                          >  2:2184234/4‑150 (MQ=255)
                                                                                                         tGGTTAAGTTGGGTAAATATAAACAGCGTGTCGAAATTGCCTTTTTCTACTGTGTGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGAAGATAAAAACAGCGCCCGCGTAt                                         <  1:640578/150‑1 (MQ=255)
                                                                                                         tGGTGAAGTTGGGTAAAGATAAATAGCGCGTTGAAATTGCCCTTTTTTACTGTGAGTTAGCCCTGCAGCATATGGCAAGCGACGATATCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCATATAAAAACAGCGCCCGCGTAt                                         <  1:3232797/150‑1 (MQ=255)
                                                                                                         tGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAt                                         >  1:3433107/1‑150 (MQ=255)
                                                                                                         tGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATAGGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAt                                         <  1:1683157/150‑1 (MQ=255)
                                                                                                           gTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATcc                                       >  2:3202613/1‑150 (MQ=255)
                                                                                                            tGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCa                                      >  2:1967309/1‑150 (MQ=255)
                                                                                                             gAAGCTGGGTAAATATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCAt                                     <  1:1419783/150‑1 (MQ=255)
                                                                                                                 cTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatg                                 >  1:1638518/1‑150 (MQ=255)
                                                                                                                  tGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTCTCCATAatga                                >  1:2745413/1‑150 (MQ=255)
                                                                                                                  tGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatga                                >  1:869355/1‑150 (MQ=255)
                                                                                                                  tGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatga                                >  1:137066/1‑150 (MQ=255)
                                                                                                                   gggTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatgat                               <  1:1735052/150‑1 (MQ=255)
                                                                                                                     gTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATgg                             <  2:2340721/150‑1 (MQ=255)
                                                                                                                      tAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATggg                            <  2:3675804/150‑1 (MQ=255)
                                                                                                                      tAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATggg                            >  2:3397670/1‑150 (MQ=255)
                                                                                                                        aaGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAc                          <  1:3639814/150‑1 (MQ=255)
                                                                                                                             aaaCAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCgtg                     >  2:1887127/1‑150 (MQ=255)
                                                                                                                                 aGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTa                 <  2:25512/150‑1 (MQ=255)
                                                                                                                                 aGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTa                 >  1:1670566/1‑150 (MQ=255)
                                                                                                                                    gcgTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATgg              <  2:639044/150‑1 (MQ=255)
                                                                                                                                    gcgTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATgg              <  1:2281576/150‑1 (MQ=255)
                                                                                                                                     cgTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGc             >  2:421575/1‑150 (MQ=255)
                                                                                                                                       tCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATTAAAACAGCGCCCGCGTATCCATAATTATTGGACGAGTGTTTATGGCGa           <  2:2677411/150‑1 (MQ=255)
                                                                                                                                        cGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGa           >  1:1095938/1‑150 (MQ=255)
                                                                                                                                         gAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGaaa         >  1:578605/1‑150 (MQ=255)
                                                                                                                                         gAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGaaa         >  2:1299672/1‑150 (MQ=255)
                                                                                                                                          aaaTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAg        <  1:80402/150‑1 (MQ=255)
                                                                                                                                          aaaTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAg        >  1:1696724/1‑150 (MQ=255)
                                                                                                                                           aaTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAgg       <  2:2368532/150‑1 (MQ=255)
                                                                                                                                            aTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGga      <  1:152879/150‑1 (MQ=255)
                                                                                                                                            aTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGga      <  1:2062520/150‑1 (MQ=255)
                                                                                                                                             ttGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGgag     <  1:1875437/150‑1 (MQ=255)
                                                                                                                                                cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGTCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAt  >  2:1403786/1‑150 (MQ=255)
                                                                                                                                                cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATGATGATGGGACGCGTGTTTATGGCGAAAGGAGAAt  <  2:3163678/150‑1 (MQ=255)
                                                                                                                                                cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAt  <  1:3123791/150‑1 (MQ=255)
                                                                                                                                                cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAt  <  2:1394431/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAT  >  CP009273/1335209‑1335502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑