BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,335,922	C→T	H370Y (CAT→TAT)	yciM →	envelope integrity maintenance protein; EnvC‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,335,922	0	C	T	100.0%	652.9 / NA	194	H370Y (CAT→TAT)	yciM	envelope integrity maintenance protein; EnvC‑interacting protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (84/110); total (84/110)

Only 100 of 194 total aligned reads displayed.

TTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAG  >  CP009273/1335775‑1336067

ttAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTAt                                                                                                                                                  >  1:2237334/1‑150 (MQ=255)

  aaTGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTg                                                                                                                                                >  2:3738400/1‑150 (MQ=255)

     ggATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTcc                                                                                                                                             <  2:168049/150‑1 (MQ=255)

       aTTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGt                                                                                                                                           >  2:2456932/1‑150 (MQ=255)

         tACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCt                                                                                                                                         <  1:3468021/150‑1 (MQ=255)

            cACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGt                                                                                                                                      >  1:785651/1‑150 (MQ=255)

             aCTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTc                                                                                                                                     <  2:3590752/150‑1 (MQ=255)

                tAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCggg                                                                                                                                  <  2:1330889/150‑1 (MQ=255)

                tAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCggg                                                                                                                                  <  1:2834517/150‑1 (MQ=255)

                  aaTGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGcc                                                                                                                                >  1:1248258/1‑150 (MQ=255)

                   aTGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCt                                                                                                                               <  1:2343929/150‑1 (MQ=255)

                    tGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTg                                                                                                                              >  1:112806/1‑150 (MQ=255)

                          ggAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAAc                                                                                                                        <  1:647833/150‑1 (MQ=255)

                          ggAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAAc                                                                                                                        >  2:410024/1‑150 (MQ=255)

                             agaagGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCAt                                                                                                                     <  2:195/150‑1 (MQ=255)

                               aagGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTa                                                                                                                   >  1:546005/1‑150 (MQ=255)

                                   gCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAAcc                                                                                                               <  1:417449/150‑1 (MQ=255)

                                    cGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCg                                                                                                              >  1:1550032/1‑150 (MQ=255)

                                    cGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTATTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCg                                                                                                              >  1:2321416/1‑150 (MQ=255)

                                       gCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGAtt                                                                                                           <  1:1672267/150‑1 (MQ=255)

                                        ccAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTc                                                                                                          >  2:2397226/1‑150 (MQ=255)

                                        ccAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTc                                                                                                          <  2:502284/150‑1 (MQ=255)

                                         cAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTcg                                                                                                         <  2:1924632/150‑1 (MQ=255)

                                         cAAAGAGAGCCTGATGGAGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTcg                                                                                                         <  2:1924991/150‑1 (MQ=255)

                                          aaaGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTcgc                                                                                                        >  2:1391996/1‑150 (MQ=255)

                                          aaaGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTcgc                                                                                                        <  1:3211046/150‑1 (MQ=255)

                                           aaGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTcgcg                                                                                                       >  2:710442/1‑150 (MQ=255)

                                           aaGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTcgcg                                                                                                       <  2:1591071/150‑1 (MQ=255)

                                            agagagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCgg                                                                                                      <  2:655318/150‑1 (MQ=255)

                                            agagagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCgg                                                                                                      >  2:1102573/1‑150 (MQ=255)

                                             gagagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGt                                                                                                     >  2:3230271/1‑150 (MQ=255)

                                               gagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCt                                                                                                   >  1:2948904/1‑150 (MQ=255)

                                               gagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCt                                                                                                   <  1:1240120/150‑1 (MQ=255)

                                                agCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCtt                                                                                                  <  2:1890330/150‑1 (MQ=255)

                                                 gCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTg                                                                                                 >  1:3065273/1‑150 (MQ=255)

                                                 gCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTg                                                                                                 >  1:1166142/1‑150 (MQ=255)

                                                 gCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTg                                                                                                 >  1:1823512/1‑150 (MQ=255)

                                                  ccTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGa                                                                                                >  1:1545997/1‑150 (MQ=255)

                                                       tGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGcc                                                                                           <  2:203639/150‑1 (MQ=255)

                                                       tGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGcc                                                                                           <  1:2509478/150‑1 (MQ=255)

                                                          tgctgcGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGt                                                                                        >  2:3528913/1‑150 (MQ=255)

                                                            ctgcGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTaa                                                                                      <  1:1898879/150‑1 (MQ=255)

                                                            ctgcGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTaa                                                                                      >  2:907479/1‑150 (MQ=255)

                                                            ctgcGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTaa                                                                                      <  1:3228988/150‑1 (MQ=255)

                                                            ctgcGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTaa                                                                                      <  1:1896682/150‑1 (MQ=255)

                                                              gcGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAAtt                                                                                    <  2:2967018/150‑1 (MQ=255)

                                                                       ggTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑aaaaaa                                                                          <  1:3627036/150‑1 (MQ=255)

                                                                               agaAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGAc                                                                  >  1:27832/1‑150 (MQ=255)

                                                                               agaAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGAc                                                                  >  2:1636707/1‑150 (MQ=255)

                                                                                gaAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACt                                                                 <  2:2657519/150‑1 (MQ=255)

                                                                                gaAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACt                                                                 >  2:534641/1‑150 (MQ=255)

                                                                                 aaGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACtt                                                                <  1:3324506/150‑1 (MQ=255)

                                                                                  aGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACttt                                                               <  1:751756/150‑1 (MQ=255)

                                                                                       cGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTa                                                          <  1:702861/150‑1 (MQ=255)

                                                                                       cGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTa                                                          <  1:2201729/150‑1 (MQ=255)

                                                                                        gtagtaAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTac                                                         <  2:3145375/150‑1 (MQ=255)

                                                                                         tagtaAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTaca                                                        <  2:2313942/150‑1 (MQ=255)

                                                                                         tagtaAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTaca                                                        <  2:854635/150‑1 (MQ=255)

                                                                                          agtaAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTacaa                                                       <  2:1248134/150‑1 (MQ=255)

                                                                                               gCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCAGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATAc                                                  <  2:3547171/150‑1 (MQ=255)

                                                                                                ccTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACt                                                 >  1:2683256/1‑150 (MQ=255)

                                                                                                 cTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTa                                                >  2:1813037/1‑150 (MQ=255)

                                                                                                    gTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAatt                                             <  1:2397226/150‑1 (MQ=255)

                                                                                                     ttATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAatta                                            >  2:2989070/1‑150 (MQ=255)

                                                                                                     ttATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTATTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAatta                                            <  1:2436894/150‑1 (MQ=255)

                                                                                                      tATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCTTATTGTCGGGCCTGGTCAACCATTAAAACGATTAGCGGTCTTGATGGAATGTAATTTTT‑AAAAAAAATAAGACTTTAGTTACAACATAATAattat                                           >  1:2038232/1‑150 (MQ=255)

                                                                                                      tATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAattat                                           >  2:897975/1‑150 (MQ=255)

                                                                                                       aTCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAattatt                                          >  2:3389132/1‑150 (MQ=255)

                                                                                                       aTCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAattatt                                          <  2:3189844/150‑1 (MQ=255)

                                                                                                         cGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTaa                                        >  1:1851092/1‑150 (MQ=255)

                                                                                                          gTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAAt                                       <  2:1738566/150‑1 (MQ=255)

                                                                                                          gTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAAt                                       <  2:1529638/150‑1 (MQ=255)

                                                                                                          gTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAAt                                       >  2:1144720/1‑150 (MQ=255)

                                                                                                          gTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAAt                                       >  1:2251587/1‑150 (MQ=255)

                                                                                                           ttGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAAt                                       <  1:14179/150‑1 (MQ=255)

                                                                                                             gCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGtt                                    <  2:3428438/150‑1 (MQ=255)

                                                                                                             gCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGtt                                    <  2:1533111/150‑1 (MQ=255)

                                                                                                             gCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGtt                                    >  2:2587751/1‑150 (MQ=255)

                                                                                                              ccAGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTc                                   <  2:3681065/150‑1 (MQ=255)

                                                                                                                aGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTGATGTTCCa                                 >  2:991732/1‑150 (MQ=255)

                                                                                                                aGAAATGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCa                                 <  1:2795942/150‑1 (MQ=255)

                                                                                                                  aaaTGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCAtt                               <  2:2070081/150‑1 (MQ=255)

                                                                                                                  aaaTGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCAtt                               <  1:2564005/150‑1 (MQ=255)

                                                                                                                   aaTGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATtg                              >  1:1457625/1‑150 (MQ=255)

                                                                                                                   aaTGTGGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATtg                              <  2:2924029/150‑1 (MQ=255)

                                                                                                                     tgtgGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATtgtg                            <  2:785651/150‑1 (MQ=255)

                                                                                                                      gtgGTTTTACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGc                           <  1:861110/150‑1 (MQ=255)

                                                                                                                            ttACCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGc                     <  1:910293/150‑1 (MQ=255)

                                                                                                                              aCCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCaa                   <  1:2006774/150‑1 (MQ=255)

                                                                                                                              aCCGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCaa                   <  1:2006644/150‑1 (MQ=255)

                                                                                                                                cGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAacg                 >  2:1841164/1‑150 (MQ=255)

                                                                                                                                cGCATACACCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAacg                 >  1:1043444/1‑150 (MQ=255)

                                                                                                                                      cacCCTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGgcg           >  2:2221466/1‑150 (MQ=255)

                                                                                                                                        cccTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCa         <  1:1391996/150‑1 (MQ=255)

                                                                                                                                         ccTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAg        >  2:1142773/1‑150 (MQ=255)

                                                                                                                                         ccTCTACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAg        >  2:1257091/1‑150 (MQ=255)

                                                                                                                                           tctACTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGaa      <  1:3571947/150‑1 (MQ=255)

                                                                                                                                              aCTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGaaaaa   >  1:1933787/1‑150 (MQ=255)

                                                                                                                                              aCTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGaaaaa   >  1:454535/1‑150 (MQ=255)

                                                                                                                                              aCTGGTATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGaaaaa   >  2:2433391/1‑150 (MQ=255)

TTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAG  >  CP009273/1335775‑1336067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`