BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,358,855	G→T	G123C (GGC→TGC)	puuB →	gamma‑glutamylputrescine oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,358,855	0	G	T	100.0%	691.0 / NA	208	G123C (GGC→TGC)	puuB	gamma‑glutamylputrescine oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (110/98); total (110/98)

Only 100 of 210 total aligned reads displayed.

GTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGT  >  CP009273/1358708‑1359001

gtgAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCctgc                                                                                                                                                  >  2:1516053/1‑150 (MQ=255)

 tgAACTCCTCCAGCCGCGACATCGACGTGATCGAAACAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCctgcg                                                                                                                                                 <  2:2843686/150‑1 (MQ=255)

    aCTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGcc                                                                                                                                              >  1:2891894/1‑150 (MQ=255)

     cTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCt                                                                                                                                             <  2:835966/150‑1 (MQ=255)

         tACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGttt                                                                                                                                         <  2:3641235/150‑1 (MQ=255)

          aCAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtg                                                                                                                                        >  2:1366967/1‑150 (MQ=255)

          aCAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtg                                                                                                                                        >  2:2298190/1‑150 (MQ=255)

          aCAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGAATGCGACTACCGCCCCTGCGGCCTGTTtg                                                                                                                                        >  1:231079/1‑150 (MQ=255)

              ccGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGc                                                                                                                                    <  1:75340/150‑1 (MQ=255)

               cgcgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCg                                                                                                                                   <  2:2263002/150‑1 (MQ=255)

                 cgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGat                                                                                                                                 <  1:2577030/150‑1 (MQ=255)

                  gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatg                                                                                                                                >  1:2428262/1‑150 (MQ=255)

                  gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatg                                                                                                                                <  1:3190422/150‑1 (MQ=255)

                  gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatg                                                                                                                                >  1:284813/1‑150 (MQ=255)

                  gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatg                                                                                                                                <  1:2908032/150‑1 (MQ=255)

                     aTCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaat                                                                                                                             >  2:2470866/1‑150 (MQ=255)

                       cGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaatga                                                                                                                           <  2:1662686/150‑1 (MQ=255)

                        gACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAt                                                                                                                          <  2:138383/150‑1 (MQ=255)

                          cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATaa                                                                                                                        >  2:40826/1‑150 (MQ=255)

                           gTGATCG‑AAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                      <  2:1900576/150‑1 (MQ=255)

                            tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                      <  2:1900746/150‑1 (MQ=255)

                            tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                      <  2:2563683/150‑1 (MQ=255)

                            tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                      >  2:1931607/1‑150 (MQ=255)

                            tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                      <  2:2038954/150‑1 (MQ=255)

                            tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                      >  1:2476112/1‑150 (MQ=255)

                             gATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACa                                                                                                                     >  1:516150/1‑150 (MQ=255)

                                 gAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTc                                                                                                                 >  2:272045/1‑150 (MQ=255)

                                  aaaaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCg                                                                                                                >  1:3771630/1‑150 (MQ=255)

                                    aaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGcc                                                                                                              >  2:1846375/1‑150 (MQ=255)

                                       aGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcaca                                                                                                           <  1:3143045/150‑1 (MQ=255)

                                        gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCacac                                                                                                          >  2:2173474/1‑150 (MQ=255)

                                          tACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACtt                                                                                                        <  2:1818645/150‑1 (MQ=255)

                                          tACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACtt                                                                                                        <  1:30658/150‑1 (MQ=255)

                                          tACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACtt                                                                                                        <  1:30376/150‑1 (MQ=255)

                                          tACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACtt                                                                                                        >  2:2859521/1‑150 (MQ=255)

                                           aCGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTg                                                                                                       <  1:854923/150‑1 (MQ=255)

                                             ggCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGaa                                                                                                     >  1:3524498/1‑150 (MQ=255)

                                             ggCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGaa                                                                                                     >  1:3525846/1‑150 (MQ=255)

                                                aTGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAagag                                                                                                  >  2:1809633/1‑150 (MQ=255)

                                                aTGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAagag                                                                                                  >  1:1936306/1‑150 (MQ=255)

                                                aTGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAagag                                                                                                  <  1:1516053/150‑1 (MQ=255)

                                                 tGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGc                                                                                                 <  1:337223/150‑1 (MQ=255)

                                                 tGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGc                                                                                                 <  1:3411283/150‑1 (MQ=255)

                                                       ccGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAag                                                                                           <  2:2202775/150‑1 (MQ=255)

                                                         gCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAagag                                                                                         >  2:643701/1‑150 (MQ=255)

                                                           ccGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGaa                                                                                       <  2:3736324/150‑1 (MQ=255)

                                                             gTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACt                                                                                     >  2:2089777/1‑150 (MQ=255)

                                                             gTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACt                                                                                     >  1:91344/1‑150 (MQ=255)

                                                             gTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACt                                                                                     >  1:90653/1‑150 (MQ=255)

                                                               aTGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCCAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGACTGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTgg                                                                                   >  2:367556/1‑150 (MQ=255)

                                                                tGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTggg                                                                                  <  2:1211977/150‑1 (MQ=255)

                                                                   tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAAc                                                                               <  1:1083488/150‑1 (MQ=255)

                                                                   tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAAc                                                                               <  1:1895023/150‑1 (MQ=255)

                                                                    cGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACg                                                                              <  2:1835923/150‑1 (MQ=255)

                                                                     ggCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGc                                                                             >  2:499393/1‑150 (MQ=255)

                                                                     ggCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGc                                                                             >  2:499446/1‑150 (MQ=255)

                                                                      gcagcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCt                                                                            <  1:992126/150‑1 (MQ=255)

                                                                       cagcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTa                                                                           <  1:1725069/150‑1 (MQ=255)

                                                                         gcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGAGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACg                                                                         >  1:2407717/1‑150 (MQ=255)

                                                                          caTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACgg                                                                        >  1:3352827/1‑150 (MQ=255)

                                                                           atgatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGc                                                                       >  1:3374502/1‑150 (MQ=255)

                                                                            tgatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGACTGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCa                                                                      >  1:1100747/1‑150 (MQ=255)

                                                                              atgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAAt                                                                    >  1:2122098/1‑150 (MQ=255)

                                                                                   cGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACa                                                               <  2:3771630/150‑1 (MQ=255)

                                                                                        gtggtgAGATCATCCGAGAACGTATCAAACGTTATCAGATTGTCTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGATTGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTgg                                                          <  2:1011226/150‑1 (MQ=255)

                                                                                          ggtgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                        >  1:3377637/1‑150 (MQ=255)

                                                                                            tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAtt                                                      <  1:3203147/150‑1 (MQ=255)

                                                                                            tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAtt                                                      <  1:3386698/150‑1 (MQ=255)

                                                                                             gagaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTg                                                     <  2:1074503/150‑1 (MQ=255)

                                                                                               gaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCt                                                   <  2:3462587/150‑1 (MQ=255)

                                                                                               gaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCt                                                   <  2:1110047/150‑1 (MQ=255)

                                                                                                   atcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGac                                               <  2:3489472/150‑1 (MQ=255)

                                                                                                   atcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGac                                               >  1:2199379/1‑150 (MQ=255)

                                                                                                       gcgAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgcca                                           >  1:2967850/1‑150 (MQ=255)

                                                                                                         gAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaac                                         >  2:127760/1‑150 (MQ=255)

                                                                                                         gAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaac                                         >  1:1146019/1‑150 (MQ=255)

                                                                                                          aaCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacg                                        >  1:591743/1‑150 (MQ=255)

                                                                                                          aaCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacg                                        >  1:163863/1‑150 (MQ=255)

                                                                                                          aaCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacg                                        <  2:516150/150‑1 (MQ=255)

                                                                                                            cGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacgcc                                      <  2:1742854/150‑1 (MQ=255)

                                                                                                                tCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTc                                  >  2:3274238/1‑150 (MQ=255)

                                                                                                                 cAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCg                                 >  2:3029167/1‑150 (MQ=255)

                                                                                                                  aaaCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGc                                >  1:3512516/1‑150 (MQ=255)

                                                                                                                   aaCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGcc                               <  2:3354058/150‑1 (MQ=255)

                                                                                                                    aCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCg                              <  1:643701/150‑1 (MQ=255)

                                                                                                                      gTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTg                            >  1:1192352/1‑150 (MQ=255)

                                                                                                                      gTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTg                            >  1:1220350/1‑150 (MQ=255)

                                                                                                                         aTCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAg                         >  2:1720019/1‑150 (MQ=255)

                                                                                                                           cAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTa                       <  1:1023359/150‑1 (MQ=255)

                                                                                                                            aGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGACTGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGACCGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCCTTCGCCGTGACGTAg                      >  1:2042349/1‑150 (MQ=255)

                                                                                                                                 gACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGc                 >  2:1556348/1‑150 (MQ=255)

                                                                                                                                   cTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa               <  2:2891894/150‑1 (MQ=255)

                                                                                                                                     gCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATc             <  1:1418170/150‑1 (MQ=255)

                                                                                                                                     gCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATc             <  2:2428262/150‑1 (MQ=255)

                                                                                                                                       gACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGc           >  2:3150905/1‑150 (MQ=255)

                                                                                                                                         cTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCta         <  2:2827908/150‑1 (MQ=255)

                                                                                                                                         cTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCta         >  1:3272606/1‑150 (MQ=255)

                                                                                                                                           aCCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCtata       >  1:3715378/1‑150 (MQ=255)

                                                                                                                                             cGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATAcc     <  1:1809695/150‑1 (MQ=255)

                                                                                                                                              gCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCg    >  2:1443576/1‑150 (MQ=255)

GTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGT  >  CP009273/1358708‑1359001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`