Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,479,733 G→T D806Y (GAT→TAT)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,479,7330GT99.5% 700.3 / NA 213D806Y (GAT→TAT) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (106/106);  total (107/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

GGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAG  >  CP009273/1479586‑1479879
                                                                                                                                                   |                                                                                                                                                  
ggTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTAt                                                                                                                                                  <  2:2390345/150‑1 (MQ=255)
 gTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATg                                                                                                                                                 >  2:3248960/1‑150 (MQ=255)
 gTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATg                                                                                                                                                 <  2:1921889/150‑1 (MQ=255)
  tGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGt                                                                                                                                                >  1:725639/1‑150 (MQ=255)
      tGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtc                                                                                                                                            <  1:2688493/150‑1 (MQ=255)
       ggCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtct                                                                                                                                           <  1:2950300/150‑1 (MQ=255)
       ggCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtct                                                                                                                                           <  1:2630824/150‑1 (MQ=255)
        gCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtctc                                                                                                                                          <  2:3478629/150‑1 (MQ=255)
        ccAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAAtctc                                                                                                                                          >  2:3214625/2‑150 (MQ=255)
          aGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCg                                                                                                                                        >  2:245969/1‑150 (MQ=255)
          aGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCg                                                                                                                                        >  2:1984107/1‑150 (MQ=255)
          aGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCg                                                                                                                                        <  1:2896006/150‑1 (MQ=255)
             cgcgTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTc                                                                                                                                     <  1:2971982/150‑1 (MQ=255)
               cgTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGc                                                                                                                                   >  1:2508677/1‑150 (MQ=255)
                  cACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCAc                                                                                                                                >  1:2471534/1‑150 (MQ=255)
                    cGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACtt                                                                                                                              >  1:3579301/1‑150 (MQ=255)
                     gCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTc                                                                                                                             >  2:3253758/1‑150 (MQ=255)
                     gCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTc                                                                                                                             <  2:3737301/150‑1 (MQ=255)
                      cATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCg                                                                                                                            >  1:179135/1‑150 (MQ=255)
                       aTTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGa                                                                                                                           <  1:942701/150‑1 (MQ=255)
                       aTTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGa                                                                                                                           >  1:117760/1‑150 (MQ=255)
                          cttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                        >  1:593891/1‑150 (MQ=255)
                          cttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                        >  2:3421977/1‑150 (MQ=255)
                          cttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                        >  2:3422265/1‑150 (MQ=255)
                          cttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAg                                                                                                                        >  2:1488525/1‑150 (MQ=255)
                           ttcCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGc                                                                                                                       >  1:310718/1‑150 (MQ=255)
                                tGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCtggtg                                                                                                                  >  2:1728180/1‑150 (MQ=255)
                                   aaaCCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGaa                                                                                                               <  2:2757281/150‑1 (MQ=255)
                                     aCCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGTGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGaaaa                                                                                                             >  2:212342/1‑150 (MQ=255)
                                            aCTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAg                                                                                                      <  2:3082703/150‑1 (MQ=255)
                                               aCGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCcg                                                                                                   <  2:525779/150‑1 (MQ=255)
                                                cGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCcgc                                                                                                  >  1:3250350/1‑150 (MQ=255)
                                                     ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAc                                                                                             >  2:1490195/1‑150 (MQ=255)
                                                     ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAc                                                                                             >  1:2730318/1‑150 (MQ=255)
                                                     ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAc                                                                                             >  2:2744212/1‑150 (MQ=255)
                                                     ggAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAAc                                                                                             >  2:1583540/1‑150 (MQ=255)
                                                      gAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACg                                                                                            <  1:1281326/150‑1 (MQ=255)
                                                        aGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGATGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACATCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGcc                                                                                          >  1:2872129/1‑150 (MQ=255)
                                                          tAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTg                                                                                        <  1:2540203/150‑1 (MQ=255)
                                                          tAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTg                                                                                        >  1:201869/1‑150 (MQ=255)
                                                            gaagaGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGAt                                                                                      <  2:1862688/150‑1 (MQ=255)
                                                              agagCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGAttt                                                                                    <  1:1372160/150‑1 (MQ=255)
                                                               gagCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTg                                                                                   <  1:1706763/150‑1 (MQ=255)
                                                                    ggAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCaa                                                                              <  2:3584212/150‑1 (MQ=255)
                                                                     gAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAAc                                                                             >  1:19244/1‑150 (MQ=255)
                                                                      aaCACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACt                                                                            >  1:627977/1‑150 (MQ=255)
                                                                      aaCACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACt                                                                            <  1:2348178/150‑1 (MQ=255)
                                                                       acacAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACtt                                                                           <  2:227769/150‑1 (MQ=255)
                                                                       acacAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACtt                                                                           >  2:3128818/1‑150 (MQ=255)
                                                                            aaTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaa                                                                      <  2:411498/150‑1 (MQ=255)
                                                                            aaTCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaa                                                                      >  1:2861019/1‑150 (MQ=255)
                                                                              tCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaa                                                                    >  1:2340772/1‑150 (MQ=255)
                                                                               cACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaaa                                                                   >  2:3329750/1‑150 (MQ=255)
                                                                               cACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGaaaaaa                                                                   >  2:103520/1‑150 (MQ=255)
                                                                                  gTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCa                                                                >  1:1305621/1‑150 (MQ=255)
                                                                                   tCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCAt                                                               <  1:1700895/150‑1 (MQ=255)
                                                                                     gccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGt                                                             >  2:3721699/1‑150 (MQ=255)
                                                                                      ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGtt                                                            >  2:2947572/1‑150 (MQ=255)
                                                                                       cgcgATATTCTGGTTGATGACGAAACGTTGTGTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTg                                                           <  1:808856/150‑1 (MQ=255)
                                                                                        gcgATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGa                                                          <  1:3329750/150‑1 (MQ=255)
                                                                                         cgATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGAt                                                         >  2:656224/1‑150 (MQ=255)
                                                                                         cgATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGAt                                                         <  1:1490123/150‑1 (MQ=255)
                                                                                          gATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATc                                                        >  1:853933/1‑150 (MQ=255)
                                                                                           atatTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCa                                                       >  2:2807446/1‑150 (MQ=255)
                                                                                             atTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCaaa                                                     <  2:55880/150‑1 (MQ=255)
                                                                                                 tGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAgg                                                 >  2:1697582/1‑150 (MQ=255)
                                                                                                    ttGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGgcg                                              <  2:3538846/150‑1 (MQ=255)
                                                                                                    ttGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGgcg                                              <  2:2858932/150‑1 (MQ=255)
                                                                                                        tgaCGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGa                                          <  1:673655/150‑1 (MQ=255)
                                                                                                            gAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGaaaaa                                      >  1:1814460/1‑150 (MQ=255)
                                                                                                            gAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGaaaaa                                      <  2:3390638/150‑1 (MQ=255)
                                                                                                             aaaCGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGaaaaaa                                     >  2:1333249/1‑150 (MQ=255)
                                                                                                                ctttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCa                                  >  2:919723/3‑150 (MQ=255)
                                                                                                                cGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCa                                  <  1:3295503/150‑1 (MQ=255)
                                                                                                                 gttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCTCTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAg                                 <  1:2350191/150‑1 (MQ=255)
                                                                                                                 gttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCTAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAg                                 <  1:3214181/150‑1 (MQ=255)
                                                                                                                  ttgttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGc                                <  1:1503438/150‑1 (MQ=255)
                                                                                                                    gttTGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCaa                              <  1:1876287/150‑1 (MQ=255)
                                                                                                                       tGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCt                           <  1:487283/150‑1 (MQ=255)
                                                                                                                       tGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCt                           <  2:2591563/150‑1 (MQ=255)
                                                                                                                       tGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCt                           <  1:2533506/150‑1 (MQ=255)
                                                                                                                       tGAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCt                           <  2:576468/150‑1 (MQ=255)
                                                                                                                        gAGTTCTACGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTg                          >  1:2388454/1‑150 (MQ=255)
                                                                                                                                cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAccc                  >  2:747063/1‑150 (MQ=255)
                                                                                                                                cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAccc                  >  1:1779375/1‑150 (MQ=255)
                                                                                                                                cGACCAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAccc                  >  2:2888957/1‑150 (MQ=255)
                                                                                                                                   ccAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGaa               >  2:1158770/1‑150 (MQ=255)
                                                                                                                                   ccAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGaa               >  1:2749346/1‑150 (MQ=255)
                                                                                                                                    cAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAc              <  2:828720/150‑1 (MQ=255)
                                                                                                                                    cAGCGCATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAAc              >  1:3502354/1‑150 (MQ=255)
                                                                                                                                      gcgcATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACtt            <  2:2388454/150‑1 (MQ=255)
                                                                                                                                        gcATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCt          >  2:1606901/1‑150 (MQ=255)
                                                                                                                                         cATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTg         >  2:2233700/1‑150 (MQ=255)
                                                                                                                                         cATCAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTg         >  1:2206608/1‑150 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      >  1:3486971/1‑150 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      >  2:1834917/1‑150 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      >  1:18938/1‑150 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      <  2:2872129/150‑1 (MQ=255)
                                                                                                                                            cAGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCa      <  2:1237630/150‑1 (MQ=255)
                                                                                                                                             aGCCACTATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCAt     <  1:313800/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTGACTGGCAGACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAG  >  CP009273/1479586‑1479879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑