Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,544,432 G→T T925T (ACG→ACT)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,544,4320GT99.6% 862.5 / NA 256T925T (ACG→ACT) fdnGformate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (125/130);  minor base A (1/0);  total (126/130)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

ACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACGCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACGGTGGGTATTCCAAT  >  CP009273/1544285‑1544578
                                                                                                                                                   |                                                                                                                                                  
 cAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTg                                                                                                                                                 <  2:1907684/150‑1 (MQ=255)
  aaGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGACATCAGCGAAACTCTgg                                                                                                                                                >  2:3688189/1‑150 (MQ=255)
  aaGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTgg                                                                                                                                                <  1:1634871/150‑1 (MQ=255)
   aGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTggc                                                                                                                                               >  1:857805/1‑150 (MQ=255)
   aGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTggc                                                                                                                                               <  1:3274005/150‑1 (MQ=255)
   aGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTggc                                                                                                                                               <  2:339490/150‑1 (MQ=255)
   aGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTggc                                                                                                                                               <  1:2991086/150‑1 (MQ=255)
       gcgcTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTggcggcg                                                                                                                                           <  2:2638201/150‑1 (MQ=255)
           tGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGa                                                                                                                                       <  2:2186762/150‑1 (MQ=255)
             cGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGaaa                                                                                                                                     <  1:3022191/150‑1 (MQ=255)
             cGGATGGGTAAAAAAGAGCAGGTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGaaa                                                                                                                                     <  2:2403175/150‑1 (MQ=255)
              ggATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAg                                                                                                                                    <  1:2817858/150‑1 (MQ=255)
                  gggTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCAt                                                                                                                                <  2:1797485/150‑1 (MQ=255)
                   ggTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGACCAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCAtt                                                                                                                               <  2:1824555/150‑1 (MQ=255)
                   ggTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCAtt                                                                                                                               <  2:1412073/150‑1 (MQ=255)
                   ggTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCAtt                                                                                                                               <  1:3667156/150‑1 (MQ=255)
                   ggTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCAtt                                                                                                                               >  1:1322894/1‑150 (MQ=255)
                     tAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATtaa                                                                                                                             >  2:2606219/1‑150 (MQ=255)
                      aaaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATtaat                                                                                                                            >  2:3120142/1‑150 (MQ=255)
                       aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATgaata                                                                                                                           >  1:1709694/1‑150 (MQ=255)
                         aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTaataat                                                                                                                         >  2:448031/1‑150 (MQ=255)
                          aaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATg                                                                                                                        <  2:2106936/150‑1 (MQ=255)
                          aaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATg                                                                                                                        >  1:3089092/1‑150 (MQ=255)
                           agagCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATgg                                                                                                                       <  2:3023721/150‑1 (MQ=255)
                           agagCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATgg                                                                                                                       >  2:2472788/1‑150 (MQ=255)
                                    cccGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCgtgt                                                                                                              >  1:482084/1‑150 (MQ=255)
                                     ccGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTc                                                                                                             <  1:1754733/150‑1 (MQ=255)
                                      cGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCa                                                                                                            <  2:2909972/150‑1 (MQ=255)
                                       gTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCAc                                                                                                           >  2:1085306/1‑150 (MQ=255)
                                          tgtgGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGt                                                                                                        <  1:249791/150‑1 (MQ=255)
                                            tgGGTTCGACCTTTCGTCTGACCGAGCACGTCCTCTCGTGGTCCTAGCTTGCTTTGCTCAATGCTTTTGCTCTGCTGGTTCAGTTTGTGGATTTCAGCGATTCTCTGGCGGCGGCGTATGGCATTATTAATGGCGATCGTGTCACTGtct                                                                                                      <  1:133768/150‑1 (MQ=255)
                                            tgGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGtct                                                                                                      >  2:2267268/1‑150 (MQ=255)
                                             gggTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGtctc                                                                                                     >  1:1475722/1‑150 (MQ=255)
                                                tACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAg                                                                                                  <  1:2965236/150‑1 (MQ=255)
                                                tACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAg                                                                                                  >  1:3546265/1‑150 (MQ=255)
                                                    aCCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAg                                                                                              >  1:204609/1‑150 (MQ=255)
                                                     ccTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGc                                                                                             >  1:1023312/1‑150 (MQ=255)
                                                      cTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCg                                                                                            >  2:1559251/1‑150 (MQ=255)
                                                       tATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGt                                                                                           >  2:756197/1‑150 (MQ=255)
                                                       tATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGt                                                                                           >  1:2132135/1‑150 (MQ=255)
                                                        aTCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTg                                                                                          >  1:845629/1‑150 (MQ=255)
                                                         tCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTgg                                                                                         <  1:1870406/150‑1 (MQ=255)
                                                         tCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTgg                                                                                         <  1:640093/150‑1 (MQ=255)
                                                          cGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGc                                                                                        <  2:3512803/150‑1 (MQ=255)
                                                            tCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTg                                                                                          >  2:3206388/1‑146 (MQ=255)
                                                            tCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTg                                                                                          <  1:1304901/146‑1 (MQ=255)
                                                             cTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCttt                                                                                     <  1:2951531/150‑1 (MQ=255)
                                                              tGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTa                                                                                    <  1:2272285/150‑1 (MQ=255)
                                                              tGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTa                                                                                    <  2:2133797/150‑1 (MQ=255)
                                                               gACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTAt                                                                                   >  2:83563/1‑150 (MQ=255)
                                                               gACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTAt                                                                                   >  1:366753/1‑150 (MQ=255)
                                                                aCCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATc                                                                                  >  1:1229927/1‑150 (MQ=255)
                                                                               acCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCtgtg                                                                   >  1:2355004/1‑150 (MQ=255)
                                                                               acCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCtgtg                                                                   <  2:3742794/150‑1 (MQ=255)
                                                                               acCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCtgtg                                                                   >  2:175877/1‑150 (MQ=255)
                                                                                 cTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGt                                                                 >  2:3006510/1‑150 (MQ=255)
                                                                                   ggACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTaa                                                               <  1:1553892/150‑1 (MQ=255)
                                                                                    gACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAAc                                                              <  1:2741768/150‑1 (MQ=255)
                                                                                    gACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAAc                                                              <  2:2796363/150‑1 (MQ=255)
                                                                                     aCCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAAcg                                                             >  2:1044359/1‑150 (MQ=255)
                                                                                        aaGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGcgt                                                          <  1:270708/150‑1 (MQ=255)
                                                                                           cACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGcgtcgt                                                       >  2:1607454/1‑150 (MQ=255)
                                                                                             cGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCt                                                     >  2:205013/1‑150 (MQ=255)
                                                                                             cGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCt                                                     <  2:1442338/150‑1 (MQ=255)
                                                                                               cATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGa                                                   >  2:2710105/1‑150 (MQ=255)
                                                                                               cATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGa                                                   >  2:3777264/1‑150 (MQ=255)
                                                                                               cATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGa                                                   >  1:981936/1‑150 (MQ=255)
                                                                                                aTTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGaa                                                  <  2:3308468/150‑1 (MQ=255)
                                                                                                  tGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAAc                                                >  1:441519/1‑150 (MQ=255)
                                                                                                   gCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAAcc                                               >  1:397523/1‑150 (MQ=255)
                                                                                                   gCTCAACGCAATTGCTCAGCCGGAACAGTGTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAAcc                                               <  2:3329744/150‑1 (MQ=255)
                                                                                                      cAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCt                                            <  2:1469421/150‑1 (MQ=255)
                                                                                                      cAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCt                                            <  1:730663/150‑1 (MQ=255)
                                                                                                       aaCGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTg                                           >  2:1150245/1‑150 (MQ=255)
                                                                                                       aaCGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTg                                           >  2:3051678/1‑150 (MQ=255)
                                                                                                           cAATTGCTCAGCCGGAACAGTTTTTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATg                                       <  1:323528/150‑1 (MQ=255)
                                                                                                           cAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATg                                       <  1:708299/150‑1 (MQ=255)
                                                                                                            aaTTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGt                                      >  1:550225/1‑150 (MQ=255)
                                                                                                             aTTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTa                                     <  1:106668/150‑1 (MQ=255)
                                                                                                               tGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTaaa                                   >  2:1719622/1‑150 (MQ=255)
                                                                                                                gCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAAt                                  <  2:2758417/150‑1 (MQ=255)
                                                                                                                   cAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGt                               <  2:746096/150‑1 (MQ=255)
                                                                                                                   cAGCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGt                               >  2:1181930/1‑150 (MQ=255)
                                                                                                                     gCCGGCACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTca                             <  2:1703690/150‑1 (MQ=255)
                                                                                                                     gCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTca                             <  1:3403724/150‑1 (MQ=255)
                                                                                                                     gCCGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTca                             >  2:355868/1‑150 (MQ=255)
                                                                                                                       cGGAACAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTcagc                           >  1:1141323/1‑150 (MQ=255)
                                                                                                                            cAGTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGtt                      >  2:2359722/1‑150 (MQ=255)
                                                                                                                              gTTTGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGa                    >  2:711474/1‑150 (MQ=255)
                                                                                                                                ttGTGGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGaaa                  <  2:1278752/150‑1 (MQ=255)
                                                                                                                                 tgtgGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAAc                 >  2:1842446/1‑150 (MQ=255)
                                                                                                                                 tgtgGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAAc                 >  1:3325364/1‑150 (MQ=255)
                                                                                                                                  gtgGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACg                >  2:2968986/1‑150 (MQ=255)
                                                                                                                                  gtgGAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACg                >  2:2621225/1‑150 (MQ=255)
                                                                                                                                     gAAATCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACGGTg             <  1:3753768/150‑1 (MQ=255)
                                                                                                                                        aTCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACGGTGGGt          >  1:2622362/1‑150 (MQ=255)
                                                                                                                                        aTCAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACGGTGGGt          >  2:984182/1‑150 (MQ=255)
                                                                                                                                          cAGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACGGTGGGTAt        >  1:1531210/1‑150 (MQ=255)
                                                                                                                                           aGCGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACGGTGGGTAtt       <  2:893600/150‑1 (MQ=255)
                                                                                                                                             cGAAACTCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACGGTGGGTATTcc     <  2:550225/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACGCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTCTCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTGAATGTAAATGGTCAGCAGGTTGAAACGGTGGGTATTCCAAT  >  CP009273/1544285‑1544578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑