Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,552,629 C→A D216Y (GAT→TAT)  ddpD ← D,D‑dipeptide permease system, ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,552,6290CA99.6% 817.9 / NA 252D216Y (GAT→TAT) ddpDD,D‑dipeptide permease system, ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (147/104);  total (148/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

CTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAA  >  CP009273/1552482‑1552775
                                                                                                                                                   |                                                                                                                                                  
cTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAAt                                                                                                                                                  <  1:2543774/150‑1 (MQ=255)
 tCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGTTAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATg                                                                                                                                                 <  2:3684650/150‑1 (MQ=255)
   cATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGc                                                                                                                                               >  2:2320200/1‑150 (MQ=255)
    aTGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCt                                                                                                                                              >  1:265751/1‑150 (MQ=255)
    aTGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCt                                                                                                                                              <  1:3047622/150‑1 (MQ=255)
     tGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtg                                                                                                                                             <  1:2929921/150‑1 (MQ=255)
       gtCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgat                                                                                                                                           <  1:9687/149‑1 (MQ=255)
           ggTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAAc                                                                                                                                       <  1:1723296/150‑1 (MQ=255)
            gTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACa                                                                                                                                      <  1:1103464/150‑1 (MQ=255)
                gcATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAc                                                                                                                                  >  2:442490/1‑150 (MQ=255)
                 cATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAca                                                                                                                                 <  1:3622637/150‑2 (MQ=255)
                 cATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAca                                                                                                                                 >  1:2629275/1‑149 (MQ=255)
                 cATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAca                                                                                                                                 <  1:3622478/150‑2 (MQ=255)
                 cATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAca                                                                                                                                 >  1:484098/1‑149 (MQ=255)
                  aTTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCAGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAcag                                                                                                                                >  1:54614/1‑148 (MQ=255)
                      cagcaAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGt                                                                                                                            >  1:2943346/1‑150 (MQ=255)
                         caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTcc                                                                                                                         >  1:729992/1‑150 (MQ=255)
                         caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTcc                                                                                                                         <  2:1762253/150‑1 (MQ=255)
                         caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTcc                                                                                                                         >  2:1661197/1‑150 (MQ=255)
                         caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTcc                                                                                                                         <  1:1011741/150‑1 (MQ=255)
                          aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCa                                                                                                                        >  1:3472999/1‑150 (MQ=255)
                          aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCa                                                                                                                        >  2:3066738/1‑150 (MQ=255)
                             ccAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTg                                                                                                                     >  2:561059/1‑150 (MQ=255)
                               aaTGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGgc                                                                                                                   >  2:110921/1‑150 (MQ=255)
                                  ggTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGcgcg                                                                                                                >  1:2441021/1‑150 (MQ=255)
                                   gTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCATGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCgg                                                                                                               >  2:3796545/1‑150 (MQ=255)
                                   gTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCgg                                                                                                               >  2:281079/1‑150 (MQ=255)
                                     ataCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGc                                                                                                             <  2:1481615/150‑1 (MQ=255)
                                      taCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCt                                                                                                            <  1:1923970/150‑1 (MQ=255)
                                         ggATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGCTCCACTGGCGCGGGCTTTa                                                                                                         >  2:2877672/1‑150 (MQ=255)
                                          gATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTAt                                                                                                        <  2:3273380/150‑1 (MQ=255)
                                          gATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTAt                                                                                                        <  2:727233/150‑1 (MQ=255)
                                          gATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTAt                                                                                                        >  1:3802984/1‑150 (MQ=255)
                                            tGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGt                                                                                                      >  1:2843309/1‑150 (MQ=255)
                                             gCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGtt                                                                                                     <  1:3383100/150‑1 (MQ=255)
                                               cGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTa                                                                                                   >  1:1196015/1‑150 (MQ=255)
                                                ggggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTaa                                                                                                  >  1:2099930/1‑150 (MQ=255)
                                                  ggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGc                                                                                                >  2:2837278/1‑150 (MQ=255)
                                                      gatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAAc                                                                                            >  2:3550997/1‑150 (MQ=255)
                                                      gatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAAc                                                                                            >  1:94265/1‑150 (MQ=255)
                                                      gatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAAc                                                                                            >  2:479204/1‑150 (MQ=255)
                                                      gatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTACCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAAc                                                                                            >  2:653629/1‑150 (MQ=255)
                                                       atgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACg                                                                                           >  1:612784/1‑150 (MQ=255)
                                                       atgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACg                                                                                           <  1:1240247/150‑1 (MQ=255)
                                                       atgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACg                                                                                           <  2:2980272/150‑1 (MQ=255)
                                                         ggATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCa                                                                                         >  2:3408785/1‑150 (MQ=255)
                                                         ggATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCa                                                                                         <  2:733825/150‑1 (MQ=255)
                                                         ggATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTAACGCACAGTTGCGACACCACGGCCATAAAATGGCTAAAGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTAAGTTTAAGCAAACGCa                                                                                         >  1:199373/1‑150 (MQ=255)
                                                          gATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAg                                                                                        >  1:3138933/1‑150 (MQ=255)
                                                          gATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAg                                                                                        <  1:3607874/150‑1 (MQ=255)
                                                           aTAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGt                                                                                       <  2:2968562/150‑1 (MQ=255)
                                                                gTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTg                                                                                  <  2:2148413/150‑1 (MQ=255)
                                                                 tCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGc                                                                                 >  2:1028551/1‑150 (MQ=255)
                                                                   tGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCaa                                                                               >  2:2897795/1‑150 (MQ=255)
                                                                   tGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCaa                                                                               >  2:2897752/1‑150 (MQ=255)
                                                                          aCGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCAcc                                                                        >  2:142198/1‑150 (MQ=255)
                                                                            gccgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGt                                                                      >  2:3716898/1‑150 (MQ=255)
                                                                              cgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGa                                                                    >  2:867137/1‑150 (MQ=255)
                                                                              cgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGa                                                                    >  1:3247274/1‑150 (MQ=255)
                                                                              cgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGa                                                                    >  2:1362549/1‑150 (MQ=255)
                                                                                cTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACg                                                                  >  2:1972402/1‑150 (MQ=255)
                                                                                  ttCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTc                                                                >  2:2778055/1‑150 (MQ=255)
                                                                                   tCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTcc                                                               <  1:1329145/150‑1 (MQ=255)
                                                                                   tCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTcc                                                               >  2:3254633/1‑150 (MQ=255)
                                                                                      aTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgc                                                            >  2:3027879/1‑150 (MQ=255)
                                                                                        tACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgcgc                                                          >  2:1808363/1‑150 (MQ=255)
                                                                                         aCGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGcc                                                         <  2:3645433/150‑1 (MQ=255)
                                                                                           gCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGt                                                       >  1:812846/1‑150 (MQ=255)
                                                                                            cTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTa                                                      <  2:2300428/150‑1 (MQ=255)
                                                                                             ttCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAg                                                     <  1:442490/150‑1 (MQ=255)
                                                                                              tCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGt                                                    >  2:2805148/1‑150 (MQ=255)
                                                                                                cGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCg                                                  >  1:3265599/1‑150 (MQ=255)
                                                                                                 ggCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCgg                                                 >  2:3474824/1‑150 (MQ=255)
                                                                                                   cATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGtt                                               <  2:1618537/150‑1 (MQ=255)
                                                                                                       cATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtc                                           <  1:2776134/150‑1 (MQ=255)
                                                                                                       cATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtc                                           <  2:265751/150‑1 (MQ=255)
                                                                                                       cATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtc                                           <  2:1839345/150‑1 (MQ=255)
                                                                                                       cATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtc                                           <  1:3080165/150‑1 (MQ=255)
                                                                                                        aTCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtcg                                          <  2:2546589/150‑1 (MQ=255)
                                                                                                        aTCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtcg                                          >  1:2581634/1‑150 (MQ=255)
                                                                                                        aTCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtcg                                          <  1:1661197/150‑1 (MQ=255)
                                                                                                        aTCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtcg                                          >  2:2665676/1‑150 (MQ=255)
                                                                                                             gTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaat                                     >  1:1566424/1‑150 (MQ=255)
                                                                                                               aaaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaataa                                   >  1:1319254/1‑150 (MQ=255)
                                                                                                                aaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaataat                                  <  1:2266842/150‑1 (MQ=255)
                                                                                                                  cGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCa                                <  2:2629275/150‑1 (MQ=255)
                                                                                                                        cGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCg                          >  1:864339/1‑150 (MQ=255)
                                                                                                                         gCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCgg                         >  2:1793817/1‑150 (MQ=255)
                                                                                                                             aGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCg                     >  2:1560742/1‑150 (MQ=255)
                                                                                                                                 gCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAgg                 <  2:3138933/150‑1 (MQ=255)
                                                                                                                                  cGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGga                <  1:2169153/150‑1 (MQ=255)
                                                                                                                                   gACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGgag               >  1:763124/1‑150 (MQ=255)
                                                                                                                                   gACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGgag               >  1:3121675/1‑150 (MQ=255)
                                                                                                                                    acacCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGgaga              <  1:1799245/150‑1 (MQ=255)
                                                                                                                                      accacGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAAt            >  1:3061088/1‑150 (MQ=255)
                                                                                                                                       ccacGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATg           <  1:1437435/150‑1 (MQ=255)
                                                                                                                                        cacGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGc          >  1:367228/1‑150 (MQ=255)
                                                                                                                                          cGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCa        <  2:3084197/150‑1 (MQ=255)
                                                                                                                                           ggCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAg       >  1:375756/1‑150 (MQ=255)
                                                                                                                                             ccATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcg     >  2:2051084/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAA  >  CP009273/1552482‑1552775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑