Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,570,428 G→T I483I (ATC→ATA)  yddB ← putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,570,4280GT99.6% 778.5 / NA 242I483I (ATC→ATA) yddBputative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (133/108);  total (134/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

ATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGGTT  >  CP009273/1570281‑1570574
                                                                                                                                                   |                                                                                                                                                  
    ccTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtg                                                                                                                                              <  1:372958/150‑1 (MQ=255)
     cTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtgt                                                                                                                                             >  1:1343991/1‑150 (MQ=255)
      tGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtgtg                                                                                                                                            >  1:855842/1‑150 (MQ=255)
       gATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTgg                                                                                                                                           >  1:2430039/1‑150 (MQ=255)
       gATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTgg                                                                                                                                           >  2:198755/1‑150 (MQ=255)
        aTACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGt                                                                                                                                          >  2:576894/1‑150 (MQ=255)
         tACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGtt                                                                                                                                         <  1:298059/150‑1 (MQ=255)
            cGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGa                                                                                                                                      >  1:1484214/1‑150 (MQ=255)
               ttCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAg                                                                                                                                   >  2:353636/1‑150 (MQ=255)
                  cGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGGTTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATa                                                                                                                                <  2:636068/150‑1 (MQ=255)
                  cGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATa                                                                                                                                >  1:770205/1‑150 (MQ=255)
                   gTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAg                                                                                                                               >  2:208483/1‑150 (MQ=255)
                       aGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgt                                                                                                                           <  1:1673296/150‑1 (MQ=255)
                        gCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtc                                                                                                                          <  1:958221/150‑1 (MQ=255)
                              gCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtc                                                                                                                    >  2:279346/1‑150 (MQ=255)
                                 gATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtcata                                                                                                                 >  1:607683/1‑150 (MQ=255)
                                   tatCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATAcc                                                                                                               <  1:2740715/150‑1 (MQ=255)
                                        cGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg                                                                                                          >  2:169202/1‑150 (MQ=255)
                                        cGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg                                                                                                          <  2:3771805/150‑1 (MQ=255)
                                         gTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGc                                                                                                         >  1:2827438/1‑150 (MQ=255)
                                          tAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGcc                                                                                                        <  2:2039773/150‑1 (MQ=255)
                                          tAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGcc                                                                                                        >  2:3610070/1‑150 (MQ=255)
                                             tCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCggg                                                                                                     <  1:1662418/150‑1 (MQ=255)
                                             tCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCggg                                                                                                     >  1:1906771/1‑150 (MQ=255)
                                                cATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCAt                                                                                                  <  2:2738771/150‑1 (MQ=255)
                                                 atatCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCAtt                                                                                                 <  1:3080061/150‑1 (MQ=255)
                                                     cAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATg                                                                                             >  1:1999980/1‑150 (MQ=255)
                                                     cAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATg                                                                                             <  2:1554512/150‑1 (MQ=255)
                                                     cAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGAGAGTTGTCATAGTCATACCGCACGCCGGGCATTAATg                                                                                             >  2:2185034/1‑150 (MQ=255)
                                                      aaGAATAGTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGa                                                                                            <  2:2195407/150‑1 (MQ=255)
                                                       aGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGac                                                                                           >  2:414094/1‑150 (MQ=255)
                                                        gAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGaca                                                                                          <  2:2485565/150‑1 (MQ=255)
                                                        gAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGaca                                                                                          >  2:2066388/1‑150 (MQ=255)
                                                        gAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGaca                                                                                          >  2:2206184/1‑150 (MQ=255)
                                                         aaTATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGacac                                                                                         >  1:2439537/1‑150 (MQ=255)
                                                         aaTATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGacac                                                                                         >  1:2671403/1‑150 (MQ=255)
                                                          atatTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGAcaca                                                                                        >  2:3755130/1‑150 (MQ=255)
                                                            atTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACAtt                                                                                      >  1:2584424/1‑150 (MQ=255)
                                                            atTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACAtt                                                                                      >  1:2898893/1‑150 (MQ=255)
                                                            atTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCAGTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACAtt                                                                                      <  1:3184383/150‑1 (MQ=255)
                                                                      tAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTa                                                                            <  1:3766909/150‑1 (MQ=255)
                                                                       aGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTaa                                                                           >  2:3252644/1‑150 (MQ=255)
                                                                         tAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATg                                                                         <  2:1300787/150‑1 (MQ=255)
                                                                         tAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATg                                                                         >  2:3338253/1‑150 (MQ=255)
                                                                         tAACGGTTATAACCTGCTGTAATCATTGAGGGTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATg                                                                         >  2:3337913/1‑150 (MQ=255)
                                                                                 ataACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGc                                                                 >  1:2774879/1‑150 (MQ=255)
                                                                                  tnACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGcc                                                                >  1:1752622/1‑150 (MQ=255)
                                                                                   aaCCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCa                                                               <  1:934074/150‑1 (MQ=255)
                                                                                     ccTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCata                                                             >  2:3769175/1‑150 (MQ=255)
                                                                                      ctgctgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCatat                                                            >  2:2662225/1‑150 (MQ=255)
                                                                                       tgctgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCAtata                                                           <  2:1504677/150‑1 (MQ=255)
                                                                                        gctgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATAc                                                          >  1:494374/1‑150 (MQ=255)
                                                                                            tAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCg                                                      >  1:3331175/1‑150 (MQ=255)
                                                                                              aTCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtg                                                    <  1:1372029/150‑1 (MQ=255)
                                                                                              aTCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtg                                                    <  1:198755/150‑1 (MQ=255)
                                                                                                cATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTa                                                  >  2:305533/1‑150 (MQ=255)
                                                                                                   tGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGtt                                               <  2:2180607/150‑1 (MQ=255)
                                                                                                   tGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGtt                                               >  2:1183587/1‑150 (MQ=255)
                                                                                                   tGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGtt                                               >  1:2345247/1‑150 (MQ=255)
                                                                                                     aGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGt                                             >  1:1021409/1‑150 (MQ=255)
                                                                                                       gTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCa                                           >  1:1306129/1‑150 (MQ=255)
                                                                                                       gTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCa                                           >  2:78610/1‑150 (MQ=255)
                                                                                                         ttGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAt                                         >  1:725830/1‑150 (MQ=255)
                                                                                                            aTTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTcc                                      >  1:1420839/1‑150 (MQ=255)
                                                                                                            aTTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTcc                                      >  2:3084826/1‑150 (MQ=255)
                                                                                                            aTTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTcc                                      >  1:3778988/1‑150 (MQ=255)
                                                                                                              tAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCa                                    <  1:2362714/150‑1 (MQ=255)
                                                                                                                gCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGACATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAgg                                  >  1:1585691/1‑150 (MQ=255)
                                                                                                                     aaTATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcc                             >  2:1416863/1‑150 (MQ=255)
                                                                                                                      atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcct                            <  2:1628030/150‑1 (MQ=255)
                                                                                                                      atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcct                            <  1:1183587/150‑1 (MQ=255)
                                                                                                                          cccATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttac                        >  2:2844417/1‑150 (MQ=255)
                                                                                                                            cATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                      <  2:467837/150‑1 (MQ=255)
                                                                                                                            cATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                      >  1:1113162/1‑150 (MQ=255)
                                                                                                                             aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacctt                     >  2:733816/1‑150 (MQ=255)
                                                                                                                             aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacctt                     <  1:2168825/150‑1 (MQ=255)
                                                                                                                              ttCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttt                    <  1:1693099/150‑1 (MQ=255)
                                                                                                                              ttCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttt                    <  1:2983676/150‑1 (MQ=255)
                                                                                                                               tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttta                   >  1:617789/1‑150 (MQ=255)
                                                                                                                               tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttta                   >  1:3402336/1‑150 (MQ=255)
                                                                                                                                 cGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcc                             >  2:763618/1‑138 (MQ=255)
                                                                                                                                 cGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATg                 <  2:832725/150‑1 (MQ=255)
                                                                                                                                 cGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATg                 >  2:295889/1‑150 (MQ=255)
                                                                                                                                 cGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATg                 <  1:2189524/150‑1 (MQ=255)
                                                                                                                                 cGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATg                 <  1:279346/150‑1 (MQ=255)
                                                                                                                                  gTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg                >  2:1794789/1‑150 (MQ=255)
                                                                                                                                  gTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg                <  1:3609671/150‑1 (MQ=255)
                                                                                                                                   tCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGt               <  2:2475973/150‑1 (MQ=255)
                                                                                                                                     aTAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTaa             >  2:2266463/1‑150 (MQ=255)
                                                                                                                                       aaaGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAat           >  1:2323973/1‑150 (MQ=255)
                                                                                                                                       aaaGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAat           >  1:1224890/1‑150 (MQ=255)
                                                                                                                                         aGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGCCATACCGCACGCCGGGTATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatgg         >  2:1775739/1‑150 (MQ=255)
                                                                                                                                          gcgcGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggt        <  2:1484167/150‑1 (MQ=255)
                                                                                                                                             cgGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatg     <  1:3324355/150‑1 (MQ=255)
                                                                                                                                              ggggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg    >  1:3203863/1‑150 (MQ=255)
                                                                                                                                              ggggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg    >  1:1834967/1‑150 (MQ=255)
                                                                                                                                              ggggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg    <  1:576894/150‑1 (MQ=255)
                                                                                                                                               gggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatggt   >  2:1220883/1‑150 (MQ=255)
                                                                                                                                                ggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGGtt  <  1:3236321/150‑1 (MQ=255)
                                                                                                                                                ggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGGtt  <  2:3299398/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGGTT  >  CP009273/1570281‑1570574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑