Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,575,339 G→T F481L (TTC→TTA)  ydeN ← putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,575,3390GT100.0% 809.0 / NA 241F481L (TTC→TTA) ydeNputative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (109/132);  total (109/132)

Only 100 of 242 total aligned reads displayed.

TACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGG  >  CP009273/1575192‑1575485
                                                                                                                                                   |                                                                                                                                                  
tACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATaa                                                                                                                                                  >  2:1621996/1‑150 (MQ=255)
 aCCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAAt                                                                                                                                                 <  2:1540386/150‑1 (MQ=255)
  ccACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAAtt                                                                                                                                                <  1:832817/150‑1 (MQ=255)
   cACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTg                                                                                                                                               >  1:2438780/1‑150 (MQ=255)
   cACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTg                                                                                                                                               >  2:3020418/1‑150 (MQ=255)
    aCGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTgg                                                                                                                                              <  2:3743876/150‑1 (MQ=255)
    aCGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTgg                                                                                                                                              >  1:1822469/1‑150 (MQ=255)
      gCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCt                                                                                                                                            <  1:543313/150‑1 (MQ=255)
        cTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTa                                                                                                                                          <  2:2302085/150‑1 (MQ=255)
        cTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTa                                                                                                                                          <  1:1536092/150‑1 (MQ=255)
         ttGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTaa                                                                                                                                         <  1:465923/150‑1 (MQ=255)
          tGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAg                                                                                                                                        <  2:613281/150‑1 (MQ=255)
          tGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAg                                                                                                                                        <  2:2379429/150‑1 (MQ=255)
            cATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTc                                                                                                                                      >  2:3624070/1‑150 (MQ=255)
              tctcTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCt                                                                                                                                    >  1:3595754/1‑150 (MQ=255)
               ctctTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTc                                                                                                                                   <  2:3605939/150‑1 (MQ=255)
                 ctTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAg                                                                                                                                 <  2:1380440/150‑1 (MQ=255)
                 ctTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAg                                                                                                                                 <  1:2750879/150‑1 (MQ=255)
                        gACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTggg                                                                                                                          >  2:837139/1‑150 (MQ=255)
                          ccTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGt                                                                                                                        <  2:507398/150‑1 (MQ=255)
                             gCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTAt                                                                                                                     <  1:2387021/150‑1 (MQ=255)
                              cGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATg                                                                                                                    <  2:2765908/150‑1 (MQ=255)
                                gATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCg                                                                                                                  <  1:2231248/150‑1 (MQ=255)
                                gATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCg                                                                                                                  >  1:2347539/1‑150 (MQ=255)
                                gATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCg                                                                                                                  >  1:3097706/1‑150 (MQ=255)
                                   tGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGt                                                                                                               <  2:3223680/150‑1 (MQ=255)
                                    ggcggcggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTa                                                                                                              <  1:2693769/150‑1 (MQ=255)
                                    ggcggcggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTa                                                                                                              <  1:94563/150‑1 (MQ=255)
                                      cggcggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAAt                                                                                                            >  1:3482793/1‑150 (MQ=255)
                                      cggcggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAAt                                                                                                            >  1:384404/1‑150 (MQ=255)
                                       ggcggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATc                                                                                                           <  2:3378037/150‑1 (MQ=255)
                                       ggcggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATc                                                                                                           <  2:3592001/150‑1 (MQ=255)
                                         cggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGt                                                                                                         >  2:208191/1‑150 (MQ=255)
                                         cggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGt                                                                                                         >  2:2905237/1‑150 (MQ=255)
                                           gcAAGGTTATCTTTTTGATGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTct                                                                                                       <  1:795184/150‑1 (MQ=255)
                                             aaGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTctga                                                                                                     >  2:2983393/1‑150 (MQ=255)
                                             aaGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTctga                                                                                                     >  1:642288/1‑150 (MQ=255)
                                               ggTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTctgact                                                                                                   <  2:2355418/150‑1 (MQ=255)
                                                gTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTctgactg                                                                                                  <  1:1190795/150‑1 (MQ=255)
                                                   aTCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATg                                                                                               >  1:1071525/1‑150 (MQ=255)
                                                   aTCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATg                                                                                               >  2:2110343/1‑150 (MQ=255)
                                                   aTCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATg                                                                                               >  1:1617457/1‑150 (MQ=255)
                                                         ttGCTGTAGATCCGTCAGTTTGTAGAGAACTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAAc                                                                                         <  1:257415/150‑1 (MQ=255)
                                                             tGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAAt                                                                                     <  1:1253759/150‑1 (MQ=255)
                                                               tAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAAttt                                                                                   <  2:3174148/150‑1 (MQ=255)
                                                               tAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAAttt                                                                                   <  2:1159434/150‑1 (MQ=255)
                                                                aGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTtg                                                                                  >  2:935985/1‑150 (MQ=255)
                                                                  aTCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTtgtg                                                                                >  2:2823158/1‑150 (MQ=255)
                                                                     cGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTa                                                                             <  2:1712471/150‑1 (MQ=255)
                                                                     cGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTa                                                                             <  1:3020418/150‑1 (MQ=255)
                                                                     cGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTa                                                                             >  1:2112368/1‑150 (MQ=255)
                                                                      gTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTaa                                                                            >  2:1953360/1‑150 (MQ=255)
                                                                       tCAGTTTGTAGAGACCTAACTGATTGTTTTCTTCTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAAt                                                                           <  1:915518/150‑1 (MQ=255)
                                                                       tCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAAt                                                                           <  1:2300400/150‑1 (MQ=255)
                                                                        cAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAAtt                                                                          <  1:2905237/150‑1 (MQ=255)
                                                                         aGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTa                                                                         <  2:3660266/150‑1 (MQ=255)
                                                                         aGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTa                                                                         <  1:1073786/150‑1 (MQ=255)
                                                                               tAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGTCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAg                                                                   <  1:327260/150‑1 (MQ=255)
                                                                                agagACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCACAGa                                                                  >  2:533557/1‑150 (MQ=255)
                                                                                 gagaCCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGaa                                                                 >  1:2491480/1‑150 (MQ=255)
                                                                                       tAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAat                                                           >  1:3305399/1‑150 (MQ=255)
                                                                                         aCTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAatat                                                         >  1:862311/1‑150 (MQ=255)
                                                                                         aCTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAatat                                                         <  2:261491/150‑1 (MQ=255)
                                                                                          cTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATAtt                                                        >  2:2194525/1‑150 (MQ=255)
                                                                                               tGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCt                                                   >  2:639984/1‑150 (MQ=255)
                                                                                                gTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTc                                                  >  2:344851/1‑150 (MQ=255)
                                                                                                gTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTc                                                  >  1:2447980/1‑150 (MQ=255)
                                                                                                gTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTc                                                  <  1:408032/150‑1 (MQ=255)
                                                                                                 ttttCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCg                                                 <  2:2536054/150‑1 (MQ=255)
                                                                                                   ttCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTc                                               >  1:1585132/1‑150 (MQ=255)
                                                                                                   ttCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTT‑GGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCa                                              <  2:52450/150‑1 (MQ=255)
                                                                                                         tGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCa                                         <  2:2438780/150‑1 (MQ=255)
                                                                                                          gTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAg                                        <  2:1411817/150‑1 (MQ=255)
                                                                                                           tataGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGt                                       >  1:482600/1‑150 (MQ=255)
                                                                                                           tataGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGt                                       >  1:1423580/1‑150 (MQ=255)
                                                                                                               gACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTgaga                                   >  2:3181482/1‑150 (MQ=255)
                                                                                                               gACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTgaga                                   <  1:1953360/150‑1 (MQ=255)
                                                                                                                aCAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGaa                                  <  2:2241110/150‑1 (MQ=255)
                                                                                                                 cAAGCGAATAATCGTTATTTCTCACCGTGTAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAAt                                 <  1:3006215/150‑1 (MQ=255)
                                                                                                                 cAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAAt                                 <  2:1731030/150‑1 (MQ=255)
                                                                                                                 cAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAAt                                 <  1:2184131/150‑1 (MQ=255)
                                                                                                                  aaGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATa                                >  2:3305840/1‑150 (MQ=255)
                                                                                                                  aaGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATa                                <  2:1300027/150‑1 (MQ=255)
                                                                                                                     cGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAaga                             <  1:3283422/150‑1 (MQ=255)
                                                                                                                        ataatCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGt                          >  2:1003937/1‑150 (MQ=255)
                                                                                                                         taatCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGtt                         <  2:1239875/150‑1 (MQ=255)
                                                                                                                          aatCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTa                        >  1:2250166/1‑150 (MQ=255)
                                                                                                                             cGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATcc                     >  1:150996/1‑150 (MQ=255)
                                                                                                                             cGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATcc                     >  1:3682852/1‑150 (MQ=255)
                                                                                                                              gTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCa                    >  1:564903/1‑150 (MQ=255)
                                                                                                                               ttATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAg                   <  2:3783022/150‑1 (MQ=255)
                                                                                                                                tATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAgg                  >  2:1329432/1‑150 (MQ=255)
                                                                                                                                 aTTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGt                 >  2:2710816/1‑150 (MQ=255)
                                                                                                                                    tctcACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGACCAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAg              >  1:3129721/1‑150 (MQ=255)
                                                                                                                                     ctcACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGa             >  2:3209516/1‑150 (MQ=255)
                                                                                                                                        aCCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAttt          <  1:208191/150‑1 (MQ=255)
                                                                                                                                         ccGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAtttt         <  1:3624070/150‑1 (MQ=255)
                                                                                                                                            tataAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAt      <  1:3649216/150‑1 (MQ=255)
                                                                                                                                             ataAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAtg     <  1:1002499/150‑1 (MQ=255)
                                                                                                                                                agatAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTgg  <  2:3529746/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGG  >  CP009273/1575192‑1575485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑