Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,680,642 C→A E68D (GAG→GAT)  fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,680,6420CA99.5% 711.3 / NA 216E68D (GAG→GAT) fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (121/94);  total (122/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

TAATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTT  >  CP009273/1680495‑1680788
                                                                                                                                                   |                                                                                                                                                  
tAATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTtatc                                                                                                                                                  >  1:2757166/1‑150 (MQ=255)
 aaTTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTatct                                                                                                                                                 >  2:1818837/1‑150 (MQ=255)
 aaTTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTatct                                                                                                                                                 >  2:374828/1‑150 (MQ=255)
  aTTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCtt                                                                                                                                                <  1:2902337/150‑1 (MQ=255)
  aTTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCtt                                                                                                                                                <  2:2779040/150‑1 (MQ=255)
   ttCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTc                                                                                                                                               <  1:2222070/150‑1 (MQ=255)
     cACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAg                                                                                                                                             >  1:1343131/1‑150 (MQ=255)
     cACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAg                                                                                                                                             >  1:1343634/1‑150 (MQ=255)
        tGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGaca                                                                                                                                          >  2:2407649/1‑150 (MQ=255)
         ggCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGacaa                                                                                                                                         >  1:1458548/1‑150 (MQ=255)
             cGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAg                                                                                                                                     >  2:2488033/1‑150 (MQ=255)
                 tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa                                                                                                                                 <  2:2206741/150‑1 (MQ=255)
                 tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa                                                                                                                                 >  1:2686781/1‑150 (MQ=255)
                      aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCt                                                                                                                            >  2:2211112/1‑150 (MQ=255)
                      aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCt                                                                                                                            >  2:2211442/1‑150 (MQ=255)
                       gCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCtt                                                                                                                           <  2:2092858/150‑1 (MQ=255)
                        cACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTc                                                                                                                          <  2:3252599/150‑1 (MQ=255)
                         aCTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCa                                                                                                                         >  2:3301278/1‑150 (MQ=255)
                            tCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTa                                                                                                                      <  2:1582312/150‑1 (MQ=255)
                            tCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTa                                                                                                                      <  1:1227050/150‑1 (MQ=255)
                             cGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTaa                                                                                                                     <  2:2320000/150‑1 (MQ=255)
                                tcatgttcatgtTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACtt                                                                                                                  >  1:3279835/1‑150 (MQ=255)
                                   tgttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTg                                                                                                               >  2:2939348/1‑150 (MQ=255)
                                    gttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGc                                                                                                              <  2:2686781/150‑1 (MQ=255)
                                    gttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGc                                                                                                              >  1:513273/1‑150 (MQ=255)
                                          gttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGc                                                                                                        <  1:667838/150‑1 (MQ=255)
                                          gttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGc                                                                                                        <  1:2575095/150‑1 (MQ=255)
                                           ttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCa                                                                                                       >  2:550591/1‑150 (MQ=255)
                                            tACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCAc                                                                                                      >  1:2247401/1‑150 (MQ=255)
                                            tACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCAc                                                                                                      >  2:1053334/1‑150 (MQ=255)
                                             aCTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGTCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACg                                                                                                     <  1:2267778/150‑1 (MQ=255)
                                                 tGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTg                                                                                                 <  2:2478388/150‑1 (MQ=255)
                                                  gCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTgg                                                                                                <  1:2122478/150‑1 (MQ=255)
                                                   cGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGt                                                                                               <  2:703909/150‑1 (MQ=255)
                                                    gTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGtt                                                                                              >  2:1861131/1‑150 (MQ=255)
                                                        cGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcg                                                                                          >  1:3314883/1‑150 (MQ=255)
                                                         ggAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc                                                                                         >  1:459476/1‑150 (MQ=255)
                                                         ggAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc                                                                                         >  1:1031450/1‑150 (MQ=255)
                                                         ggAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc                                                                                         >  1:3272720/1‑150 (MQ=255)
                                                          gAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGcc                                                                                        >  1:2969593/1‑150 (MQ=255)
                                                           aGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCa                                                                                       <  2:898420/150‑1 (MQ=255)
                                                             ccGGTCTGCCAGATAGCCAGCGGGATTTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgc                                                                                     <  2:175828/150‑1 (MQ=255)
                                                             ccGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgc                                                                                     <  2:2086982/150‑1 (MQ=255)
                                                              cGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgcg                                                                                    >  2:2474314/1‑150 (MQ=255)
                                                              cGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgcg                                                                                    >  2:1409227/1‑150 (MQ=255)
                                                               ggTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgcgc                                                                                   >  1:357596/1‑150 (MQ=255)
                                                               ggTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgcgc                                                                                   >  2:2299275/1‑150 (MQ=255)
                                                                  cTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATg                                                                                >  2:1240315/1‑150 (MQ=255)
                                                                     ccAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAAt                                                                             >  1:499289/1‑150 (MQ=255)
                                                                      cAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATc                                                                            >  2:1320124/1‑150 (MQ=255)
                                                                       aGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCa                                                                           <  2:3159658/150‑1 (MQ=255)
                                                                           aGCCAGCGGGAATTCGTCGTCATTCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTTCACGCTTGGTTAGCGACAGCGCATGAATCAGTGa                                                                       <  2:2971056/150‑1 (MQ=255)
                                                                           aGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGa                                                                       <  2:859528/150‑1 (MQ=255)
                                                                           aGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGa                                                                       <  2:2232995/150‑1 (MQ=255)
                                                                            gCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAg                                                                      <  1:2108989/150‑1 (MQ=255)
                                                                              cAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGt                                                                    >  2:2410848/1‑150 (MQ=255)
                                                                                gCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTgg                                                                  >  2:2635929/1‑150 (MQ=255)
                                                                                 cGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTggg                                                                 <  1:501465/150‑1 (MQ=255)
                                                                                  gggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGc                                                                >  1:3759127/1‑150 (MQ=255)
                                                                                       ttCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAtttt                                                           <  2:1855917/150‑1 (MQ=255)
                                                                                         cgtcgtCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTtct                                                         <  2:3528148/150‑1 (MQ=255)
                                                                                         cgtcgtCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTtct                                                         <  1:1099198/150‑1 (MQ=255)
                                                                                          gtcgtcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTtctc                                                        >  1:2551287/1‑150 (MQ=255)
                                                                                             gtcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGt                                                     >  1:1654390/1‑150 (MQ=255)
                                                                                               cATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCg                                                   >  2:3596528/1‑150 (MQ=255)
                                                                                                aTGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGa                                                  >  1:1731163/1‑150 (MQ=255)
                                                                                                aTGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGa                                                  >  2:3033565/1‑150 (MQ=255)
                                                                                                   cTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAAt                                               <  2:2643685/150‑1 (MQ=255)
                                                                                                            cAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATg                                      >  2:865297/1‑150 (MQ=255)
                                                                                                                   tCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagc                               >  1:897863/1‑150 (MQ=255)
                                                                                                                    cATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcg                              <  2:1676708/150‑1 (MQ=255)
                                                                                                                    cATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcg                              >  1:1845306/1‑150 (MQ=255)
                                                                                                                     aTCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcga                             <  1:1422937/150‑1 (MQ=255)
                                                                                                                     aTCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcga                             >  1:1812249/1‑150 (MQ=255)
                                                                                                                         gccgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGt                         <  1:1572144/150‑1 (MQ=255)
                                                                                                                          ccgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGtt                        <  1:1053334/150‑1 (MQ=255)
                                                                                                                           cgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTg                       >  2:1468160/1‑150 (MQ=255)
                                                                                                                           cgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTg                       >  2:1505164/1‑150 (MQ=255)
                                                                                                                            gccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGa                      <  1:3301278/150‑1 (MQ=255)
                                                                                                                             ccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAg                     <  2:378922/150‑1 (MQ=255)
                                                                                                                              cTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGt                    >  1:1791355/1‑150 (MQ=255)
                                                                                                                              cTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGt                    <  2:837340/150‑1 (MQ=255)
                                                                                                                               tGACGAATGTCGCTCGCTTTATCTTCCGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGtt                   <  1:1121728/150‑1 (MQ=255)
                                                                                                                               tGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGtt                   <  2:2430152/150‑1 (MQ=255)
                                                                                                                               tGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTGGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGtt                   <  2:2019014/150‑1 (MQ=255)
                                                                                                                                 aCGAATGGCGCTCTCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTtg                 <  2:2589163/150‑1 (MQ=255)
                                                                                                                                 aCGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTtg                 >  1:1867416/1‑150 (MQ=255)
                                                                                                                                  cGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTtgt                >  1:2434708/1‑150 (MQ=255)
                                                                                                                                  cGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTtgt                >  1:977589/1‑150 (MQ=255)
                                                                                                                                   gAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTtgtg               >  2:129780/1‑150 (MQ=255)
                                                                                                                                    aaTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgc              >  2:3044999/1‑150 (MQ=255)
                                                                                                                                    aaTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgc              <  1:2006235/150‑1 (MQ=255)
                                                                                                                                     aTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgcg             >  1:3750181/1‑150 (MQ=255)
                                                                                                                                     aTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgcg             <  2:1389333/150‑1 (MQ=255)
                                                                                                                                       ggCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGcc           >  2:3625407/1‑150 (MQ=255)
                                                                                                                                       ggCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCCCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGcc           <  2:607507/150‑1 (MQ=255)
                                                                                                                                        gcgcTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGccc          >  1:3589087/1‑150 (MQ=255)
                                                                                                                                          gctcgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCca        >  1:3619886/1‑150 (MQ=255)
                                                                                                                                             cgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAg     >  1:546913/1‑150 (MQ=255)
                                                                                                                                                tttATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCtt  >  1:146922/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTT  >  CP009273/1680495‑1680788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑