BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,777,451	C→A	A55E (GCG→GAG)	fadK →	short chain acyl‑CoA synthetase, anaerobic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,777,451	0	C	A	100.0%	781.9 / NA	231	A55E (GCG→GAG)	fadK	short chain acyl‑CoA synthetase, anaerobic
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (90/141); total (90/141)

Only 100 of 235 total aligned reads displayed.

CGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGC  >  CP009273/1777304‑1777597

cGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAgcgagc                                                                                                                                                  <  2:2451617/150‑1 (MQ=255)

cGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAgcgagc                                                                                                                                                  <  2:544347/150‑1 (MQ=255)

 gTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCt                                                                                                                                                 >  2:809205/1‑150 (MQ=255)

 gTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCt                                                                                                                                                 >  2:2879841/1‑150 (MQ=255)

  tttAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTc                                                                                                                                                <  1:1094158/150‑1 (MQ=255)

  tttAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTc                                                                                                                                                <  2:2273539/150‑1 (MQ=255)

   ttAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCg                                                                                                                                               <  1:3381065/150‑1 (MQ=255)

   ttAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCg                                                                                                                                               <  1:1482356/150‑1 (MQ=255)

    tAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGa                                                                                                                                              >  2:1087566/1‑150 (MQ=255)

    tAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGa                                                                                                                                              >  2:3631867/1‑150 (MQ=255)

     aaCGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGAt                                                                                                                                             <  2:3391749/150‑1 (MQ=255)

     aaCGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGAt                                                                                                                                             >  1:434536/1‑150 (MQ=255)

        gAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCAc                                                                                                                                          <  1:715541/150‑1 (MQ=255)

           caacGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCAcgcc                                                                                                                                       >  1:3628904/1‑150 (MQ=255)

           caacGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCAcgcc                                                                                                                                       >  1:1320268/1‑150 (MQ=255)

           caacGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCAcgcc                                                                                                                                       <  1:1238138/150‑1 (MQ=255)

           caacGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCAcgcc                                                                                                                                       >  2:1718900/1‑150 (MQ=255)

              cgtcgtGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCcgcg                                                                                                                                    >  1:158967/1‑150 (MQ=255)

                tcgtGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAg                                                                                                                                  <  1:226970/150‑1 (MQ=255)

                  gtGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCt                                                                                                                                <  2:612362/150‑1 (MQ=255)

                      ggcgTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCt                                                                                                                            >  1:1012585/1‑150 (MQ=255)

                       gcgTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTg                                                                                                                           <  2:1532974/150‑1 (MQ=255)

                       gcgTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTg                                                                                                                           <  2:1875220/150‑1 (MQ=255)

                       gcgTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTg                                                                                                                           <  2:3769896/150‑1 (MQ=255)

                       gcgTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTg                                                                                                                           <  1:1046868/150‑1 (MQ=255)

                       gcgTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTg                                                                                                                           <  1:2949562/150‑1 (MQ=255)

                               tCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTg                                                                                                                   >  2:3141846/1‑150 (MQ=255)

                                 aGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGa                                                                                                                 >  1:619899/1‑150 (MQ=255)

                                   cAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGTAAACTGGATg                                                                                                               <  1:2879841/150‑1 (MQ=255)

                                   cAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATg                                                                                                               >  2:2368438/1‑150 (MQ=255)

                                   cAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATg                                                                                                               <  2:3733987/150‑1 (MQ=255)

                                    aaGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGt                                                                                                              <  2:2643091/150‑1 (MQ=255)

                                     aGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGtt                                                                                                             >  1:8170/1‑150 (MQ=255)

                                      gggTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTa                                                                                                            <  2:624233/150‑1 (MQ=255)

                                      gggTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTa                                                                                                            <  2:2319370/150‑1 (MQ=255)

                                        gTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGc                                                                                                          >  2:925480/1‑150 (MQ=255)

                                         ttATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCg                                                                                                         >  2:1934111/1‑150 (MQ=255)

                                           aTGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGaa                                                                                                       >  1:1524629/1‑150 (MQ=255)

                                             ggggCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAgg                                                                                                     >  1:3335073/1‑150 (MQ=255)

                                              gggCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAggg                                                                                                    <  2:2818099/150‑1 (MQ=255)

                                              gggCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAggg                                                                                                    <  2:1153409/150‑1 (MQ=255)

                                                      cTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAAt                                                                                            <  2:717693/150‑1 (MQ=255)

                                                        tCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCa                                                                                          <  1:3434972/150‑1 (MQ=255)

                                                          gCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAgg                                                                                        <  1:1765777/150‑1 (MQ=255)

                                                           cTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGc                                                                                       <  2:157479/150‑1 (MQ=255)

                                                            tGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCg                                                                                      <  1:3214760/150‑1 (MQ=255)

                                                            tGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCg                                                                                      <  2:1405214/150‑1 (MQ=255)

                                                             ggCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGa                                                                                     >  1:2086318/1‑150 (MQ=255)

                                                              gCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGat                                                                                    <  1:925480/150‑1 (MQ=255)

                                                              gCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGat                                                                                    <  1:950084/150‑1 (MQ=255)

                                                              gCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGat                                                                                    <  2:1191587/150‑1 (MQ=255)

                                                              gCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGat                                                                                    <  2:2453218/150‑1 (MQ=255)

                                                               ccGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTCTTGAATCAGGCGatc                                                                                   >  2:2586620/1‑150 (MQ=255)

                                                                  aTTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGatcgca                                                                                >  1:3363503/1‑150 (MQ=255)

                                                                   ttACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGatcgcat                                                                               >  1:308065/1‑150 (MQ=255)

                                                                     aCTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGatcgcatcg                                                                             <  2:411001/150‑1 (MQ=255)

                                                                         gcagcaGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCAtt                                                                         <  1:3553523/150‑1 (MQ=255)

                                                                         gcagcaGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCAtt                                                                         <  1:3130550/150‑1 (MQ=255)

                                                                           agcagACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTc                                                                       <  2:2934530/150‑1 (MQ=255)

                                                                            gcagACCGCTCGTGCGATGCCAGAAAAAATTGCCGTTGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCa                                                                      <  2:2039175/150‑1 (MQ=255)

                                                                             cagACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCaa                                                                     <  2:2241970/150‑1 (MQ=255)

                                                                               gACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACt                                                                   <  2:863605/150‑1 (MQ=255)

                                                                               gACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACt                                                                   >  1:2804617/1‑150 (MQ=255)

                                                                                aCCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTg                                                                  >  1:2052193/1‑150 (MQ=255)

                                                                                 ccGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGc                                                                 >  1:3567483/1‑150 (MQ=255)

                                                                                   gCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCct                                                               <  1:2242500/150‑1 (MQ=255)

                                                                                   gCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCct                                                               <  1:1949298/150‑1 (MQ=255)

                                                                                   gCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCct                                                               <  1:3489120/150‑1 (MQ=255)

                                                                                     tCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCctgg                                                             <  2:2697891/150‑1 (MQ=255)

                                                                                      cGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCctggc                                                            >  1:292157/1‑150 (MQ=255)

                                                                                      cGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCctggc                                                            >  1:2386411/1‑150 (MQ=255)

                                                                                        tGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCctggctg                                                          <  2:3053692/150‑1 (MQ=255)

                                                                                           gATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGgtg                                                       >  1:941274/1‑150 (MQ=255)

                                                                                             tGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGtgtg                                                     >  1:3595792/1‑150 (MQ=255)

                                                                                              gCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGa                                                    >  1:2640856/1‑150 (MQ=255)

                                                                                              gCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGa                                                    <  1:3192470/150‑1 (MQ=255)

                                                                                                cAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAAt                                                  <  1:1635534/150‑1 (MQ=255)

                                                                                                  gACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGACTGGCTGGTGTGAAttt                                                >  2:3266041/1‑150 (MQ=255)

                                                                                                   aCAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTa                                               <  2:852570/150‑1 (MQ=255)

                                                                                                        aTTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGtt                                          <  1:246175/150‑1 (MQ=255)

                                                                                                         ttGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTta                                         <  1:690313/150‑1 (MQ=255)

                                                                                                         ttGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTta                                         <  2:331678/150‑1 (MQ=255)

                                                                                                            ccGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTtatct                                      >  2:1887728/1‑150 (MQ=255)

                                                                                                             cGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTtatcta                                     <  1:3169723/150‑1 (MQ=255)

                                                                                                             cGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTtatcta                                     <  2:2982888/150‑1 (MQ=255)

                                                                                                                 gTCGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCtt                                 <  2:3571449/150‑1 (MQ=255)

                                                                                                                   cGATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTtgc                               <  1:1342569/150‑1 (MQ=255)

                                                                                                                    gATAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTtgcc                              >  1:2538073/1‑150 (MQ=255)

                                                                                                                      tAATCATGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTtgcctg                            <  1:1718900/150‑1 (MQ=255)

                                                                                                                            tGGTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGaa                      <  1:515350/150‑1 (MQ=255)

                                                                                                                              gTGCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGaaaa                    >  2:2651526/1‑150 (MQ=255)

                                                                                                                                gCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATc                  <  1:367689/150‑1 (MQ=255)

                                                                                                                                gCATCGTACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATc                  <  2:158967/150‑1 (MQ=255)

                                                                                                                                      tACACCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCa            >  1:1788987/1‑150 (MQ=255)

                                                                                                                                        cacCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGt          >  1:2040215/1‑150 (MQ=255)

                                                                                                                                        cacCTATAGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGt          >  1:586930/1‑150 (MQ=255)

                                                                                                                                            tataGCGAGCTCGATCTCGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTcc      <  1:1660859/150‑1 (MQ=255)

                                                                                                                                             ataGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCg     >  1:2669878/1‑150 (MQ=255)

                                                                                                                                               aGCGAGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTg   <  1:3006582/150‑1 (MQ=255)

                                                                                                                                                gcgagcTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGc  <  2:1389075/150‑1 (MQ=255)

CGTTTAACGAACAACGTCGTGCGGCGTATCGTCAGCAAGGGTTATGGGGCGATGCTTCGCTGGCCGATTACTGGCAGCAGACCGCTCGTGCGATGCCAGACAAAATTGCCGTGGTCGATAATCATGGTGCATCGTACACCTATAGCGCGCTCGATCACGCCGCGAGCTGTCTGGCAAACTGGATGTTAGCGAAGGGTATTGAATCAGGCGATCGCATCGCATTTCAACTGCCTGGCTGGTGTGAATTTACCGTTATCTATCTTGCCTGCCTGAAAATCGGTGCAGTTTCCGTGC  >  CP009273/1777304‑1777597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`