Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,781,790 A→T Q30L (CAA→CTA)  ppsR → bifunctional regulatory protein: PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,781,7900AT100.0% 735.8 / NA 218Q30L (CAA→CTA) ppsRbifunctional regulatory protein: PEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (108/110);  total (108/110)

Only 100 of 224 total aligned reads displayed.

CTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTC  >  CP009273/1781644‑1781936
                                                                                                                                                  |                                                                                                                                                  
cTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTAtt                                                                                                                                                 <  2:1662345/150‑1 (MQ=255)
 tgtgCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTAttt                                                                                                                                                >  1:3148066/1‑150 (MQ=255)
    gCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTccc                                                                                                                                             <  2:559922/150‑1 (MQ=255)
         tttAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCAc                                                                                                                                        >  2:3444848/1‑150 (MQ=255)
          ttAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACt                                                                                                                                       >  2:3609501/1‑150 (MQ=255)
           tAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTa                                                                                                                                      >  2:1291237/1‑150 (MQ=255)
                tATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATcagc                                                                                                                                 >  1:2447018/1‑150 (MQ=255)
                 aTGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATcagca                                                                                                                                >  2:1709761/1‑150 (MQ=255)
                       tCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCa                                                                                                                          <  2:2029277/150‑1 (MQ=255)
                        cATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCAc                                                                                                                         >  2:81384/1‑150 (MQ=255)
                        cATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCAc                                                                                                                         <  2:179642/150‑1 (MQ=255)
                         aTAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACg                                                                                                                        <  2:1754191/150‑1 (MQ=255)
                          tAGAAGTATGTCTGCATCACGGGAAGAACAAAATGGATACTGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGc                                                                                                                       <  2:3042469/150‑1 (MQ=255)
                           aGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCt                                                                                                                      <  1:3372671/150‑1 (MQ=255)
                           aGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCt                                                                                                                      <  1:1170080/150‑1 (MQ=255)
                           aGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCt                                                                                                                      >  1:1589681/1‑150 (MQ=255)
                            gAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTg                                                                                                                     <  1:3522585/150‑1 (MQ=255)
                              aTTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGcc                                                                                                                   >  1:2152797/1‑150 (MQ=255)
                              aTTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGcc                                                                                                                   >  1:3779715/1‑150 (MQ=255)
                                 aTGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGtt                                                                                                                <  2:1811898/150‑1 (MQ=255)
                                 aTGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGtt                                                                                                                <  1:2628700/150‑1 (MQ=255)
                                  tGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGttt                                                                                                               <  2:2816170/150‑1 (MQ=255)
                                  tGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGttt                                                                                                               <  1:395731/150‑1 (MQ=255)
                                  tGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGttt                                                                                                               <  1:1833286/150‑1 (MQ=255)
                                  tGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGttt                                                                                                               <  1:2837179/150‑1 (MQ=255)
                                   gTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTg                                                                                                              >  2:3773531/1‑150 (MQ=255)
                                      tGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCg                                                                                                           >  2:29800/1‑150 (MQ=255)
                                      tGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCg                                                                                                           >  1:887172/1‑150 (MQ=255)
                                       gCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGa                                                                                                          <  1:743491/150‑1 (MQ=255)
                                       gCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGa                                                                                                          >  2:1442033/1‑150 (MQ=255)
                                         aTCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGaaa                                                                                                        >  2:2138974/1‑150 (MQ=255)
                                           cACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAAt                                                                                                      >  1:3715616/1‑150 (MQ=255)
                                            aCGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATg                                                                                                     >  2:742017/1‑150 (MQ=255)
                                            aCGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATg                                                                                                     <  1:3478898/150‑1 (MQ=255)
                                              gggAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATgag                                                                                                   <  2:2603968/150‑1 (MQ=255)
                                               ggAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGTCACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATgaga                                                                                                  <  1:2600578/150‑1 (MQ=255)
                                                 aagaaCAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGc                                                                                                <  2:3289627/150‑1 (MQ=255)
                                                 aagaaCAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGc                                                                                                <  2:747953/150‑1 (MQ=255)
                                                  agaaCAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGcc                                                                                               <  1:1032381/150‑1 (MQ=255)
                                                  agaaCAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGcc                                                                                               >  2:631489/1‑150 (MQ=255)
                                                    aaCAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGt                                                                                             >  2:2721300/1‑150 (MQ=255)
                                                      cAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGACAATGAGAGCCGTGc                                                                                           >  1:3314334/1‑150 (MQ=255)
                                                       aaaaTGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCa                                                                                          >  1:1679598/1‑150 (MQ=255)
                                                       aaaaTGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCa                                                                                          >  2:527357/1‑150 (MQ=255)
                                                        aaaTGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCAc                                                                                         >  1:2603538/1‑150 (MQ=255)
                                                         aaTGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCAca                                                                                        <  1:839083/150‑2 (MQ=255)
                                                           tGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACggg                                                                                      >  1:1275847/1‑150 (MQ=255)
                                                            ggATCAGGCTGTTGATCGCCACGTTTTTGAGATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGc                                                                                     <  2:3681572/150‑1 (MQ=255)
                                                              aTAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAg                                                                                   <  2:1507189/150‑1 (MQ=255)
                                                                   gCTGTTGATCGCCTCGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAg                                                                              <  1:2122342/150‑1 (MQ=255)
                                                                       ttGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATc                                                                          <  2:3199533/150‑1 (MQ=255)
                                                                        tGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCa                                                                         >  2:1892689/1‑150 (MQ=255)
                                                                         gATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAg                                                                        <  1:577178/150‑1 (MQ=255)
                                                                         gATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAg                                                                        >  2:878083/1‑150 (MQ=255)
                                                                               cACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGAc                                                                  >  1:2289125/1‑150 (MQ=255)
                                                                                     ttttATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAAtt                                                            <  1:2742919/150‑1 (MQ=255)
                                                                                     ttttATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAAtt                                                            <  1:2704862/150‑1 (MQ=255)
                                                                                     ttttATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAAtt                                                            <  1:2590941/150‑1 (MQ=255)
                                                                                      tttATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAAttt                                                           <  2:1086389/150‑1 (MQ=255)
                                                                                       ttATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTa                                                          <  1:523813/150‑1 (MQ=255)
                                                                                        tataTTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTAt                                                         <  1:804443/150‑1 (MQ=255)
                                                                                         atatTTCTTATTGTACGGAAATAACTGCTGATGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCTCGCTGCCGTTTTTCTAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATc                                                        <  2:1059244/150‑1 (MQ=255)
                                                                                          tatTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCa                                                       <  1:29800/150‑1 (MQ=255)
                                                                                           atTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCAc                                                      <  2:153131/150‑1 (MQ=255)
                                                                                            tttCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCAcc                                                     <  2:1594777/150‑1 (MQ=255)
                                                                                            tttCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCAcc                                                     >  2:1548268/1‑150 (MQ=255)
                                                                                                 gATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCAACACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACa                                                >  2:1615046/1‑150 (MQ=255)
                                                                                                   tGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAgg                                              <  2:1307583/150‑1 (MQ=255)
                                                                                                     gtaagtcAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTTCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCg                                            <  2:3690627/144‑1 (MQ=255)
                                                                                                     gTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCg                                            <  2:3639766/150‑1 (MQ=255)
                                                                                                        cGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCACTTTATCACCAGACAGGCGTgc                                         >  1:2435266/1‑150 (MQ=255)
                                                                                                        cGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTgc                                         <  2:2409933/150‑1 (MQ=255)
                                                                                                          gCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTgcgc                                       >  2:3488422/1‑150 (MQ=255)
                                                                                                          gCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTgcgc                                       >  2:1932312/1‑150 (MQ=255)
                                                                                                            aaTAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGccc                                     <  1:1319192/150‑1 (MQ=255)
                                                                                                            aaTAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGccc                                     <  1:3217947/150‑1 (MQ=255)
                                                                                                            aaTAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGccc                                     <  2:1252896/150‑1 (MQ=255)
                                                                                                             aTAACTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCg                                    >  2:853014/1‑150 (MQ=255)
                                                                                                               aaCTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCt                                  >  2:2573827/1‑150 (MQ=255)
                                                                                                               aaCTGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCt                                  <  1:1973871/150‑1 (MQ=255)
                                                                                                                  tGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGt                               >  1:1114522/1‑150 (MQ=255)
                                                                                                                  tGCGGAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGt                               >  1:2780588/1‑150 (MQ=255)
                                                                                                                      gAGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGtcttc                           <  2:2868924/150‑1 (MQ=255)
                                                                                                                       aGGTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGtcttct                          >  1:3503343/1‑150 (MQ=255)
                                                                                                                        ggTATTAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTa                         >  1:2174792/1‑150 (MQ=255)
                                                                                                                            ttAGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTcc                     >  1:2402659/1‑150 (MQ=255)
                                                                                                                              aGGACACGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGACAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCAt                   >  2:1756370/1‑150 (MQ=255)
                                                                                                                                 acacGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCgt                >  1:1328414/1‑150 (MQ=255)
                                                                                                                                  cacGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCgtg               <  1:609939/150‑1 (MQ=255)
                                                                                                                                  cacGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCgtg               <  1:2515946/150‑1 (MQ=255)
                                                                                                                                    cGCAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGtt             <  2:2211283/150‑1 (MQ=255)
                                                                                                                                      cAGTAATGTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGc           >  1:1419368/1‑150 (MQ=255)
                                                                                                                                             gTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGAt    >  1:1488265/1‑150 (MQ=255)
                                                                                                                                             gTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGAt    <  1:98434/150‑1 (MQ=255)
                                                                                                                                             gTCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGAt    <  2:887172/150‑1 (MQ=255)
                                                                                                                                              tCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGAtt   >  2:694331/1‑150 (MQ=255)
                                                                                                                                              tCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGAtt   >  1:2886347/1‑150 (MQ=255)
                                                                                                                                              tCACTATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGAtt   <  1:1442033/150‑1 (MQ=255)
                                                                                                                                               cactATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTc  >  2:380613/1‑150 (MQ=255)
                                                                                                                                               cactATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTc  <  1:2791972/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTC  >  CP009273/1781644‑1781936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑