Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,823,802 C→A V64F (GTT→TTT)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,823,8020CA99.1% 673.8 / NA 221V64F (GTT→TTT) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (134/85);  minor base T (0/2);  total (134/87)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

GATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATG  >  CP009273/1823655‑1823948
                                                                                                                                                   |                                                                                                                                                  
gATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAgg                                                                                                                                                  <  2:1366267/150‑1 (MQ=255)
       cTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACg                                                                                                                                           <  1:1111793/150‑1 (MQ=255)
         tAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGtt                                                                                                                                         <  2:3311352/150‑1 (MQ=255)
           aTTGATATCGCGATTTTATTGATCCTCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCa                                                                                                                                       <  2:1280074/150‑1 (MQ=255)
            ttGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAg                                                                                                                                      <  2:3493301/150‑1 (MQ=255)
              gATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCa                                                                                                                                    <  1:1520800/150‑1 (MQ=255)
                  tCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATg                                                                                                                                <  1:2846411/150‑1 (MQ=255)
                  tCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATg                                                                                                                                <  1:3212218/150‑1 (MQ=255)
                   cgcgATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGa                                                                                                                               >  2:2855111/1‑150 (MQ=255)
                     cgATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGaaa                                                                                                                             <  2:547838/150‑1 (MQ=255)
                      gATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAg                                                                                                                            <  2:1849589/150‑1 (MQ=255)
                       aTTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGc                                                                                                                           >  1:1090535/1‑150 (MQ=255)
                        ttttATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGcc                                                                                                                          <  2:381465/150‑1 (MQ=255)
                        ttttATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGcc                                                                                                                          <  1:1540074/150‑1 (MQ=255)
                         tttATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCcg                                                                                                                         <  2:1445491/150‑1 (MQ=255)
                         tttATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCcg                                                                                                                         <  2:1587473/150‑1 (MQ=255)
                          ttATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCcgc                                                                                                                        >  2:801398/1‑150 (MQ=255)
                           tATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCcgcg                                                                                                                       >  2:3497949/1‑150 (MQ=255)
                               gATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCa                                                                                                                   >  2:1306578/1‑150 (MQ=255)
                                atcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAg                                                                                                                  <  1:1614407/150‑1 (MQ=255)
                                atcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAg                                                                                                                  >  1:2262754/1‑150 (MQ=255)
                                 tcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgc                                                                                                                 >  1:1317399/1‑150 (MQ=255)
                                 tcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgc                                                                                                                 >  1:2551187/1‑150 (MQ=255)
                                  catcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgcg                                                                                                                >  1:3236772/1‑150 (MQ=255)
                                  catcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgcg                                                                                                                >  2:1049605/1‑150 (MQ=255)
                                    tcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGcgcg                                                                                                              <  1:1870032/150‑1 (MQ=255)
                                      gCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCTCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGa                                                                                                            >  1:584092/1‑150 (MQ=255)
                                        cGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                          >  2:2783175/1‑150 (MQ=255)
                                        cGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                          <  2:1109907/150‑1 (MQ=255)
                                        cGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                          <  2:1395634/150‑1 (MQ=255)
                                         gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                         >  1:2624538/1‑150 (MQ=255)
                                         gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                         <  2:2390390/150‑1 (MQ=255)
                                          tCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcg                                                                                                        >  2:555042/1‑150 (MQ=255)
                                            aCTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgcc                                                                                                      <  2:3690614/150‑1 (MQ=255)
                                                cGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCAc                                                                                                  >  1:1996620/1‑150 (MQ=255)
                                                 ggTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACg                                                                                                 >  2:26414/1‑150 (MQ=255)
                                                 ggTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACg                                                                                                 >  1:3572798/1‑150 (MQ=255)
                                                    tGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGc                                                                                              >  1:1623482/1‑150 (MQ=255)
                                                     gCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGTGGTTAATTCGGTTTTATTGCTTTCCAGCAGTGCGGCAATGCGTTCGACAAAGGCATGACGTTCAGAAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCt                                                                                             <  1:1778652/150‑1 (MQ=255)
                                                          ttCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCg                                                                                        <  2:594578/150‑1 (MQ=255)
                                                             ccAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGTCCGACa                                                                                     >  1:521821/1‑150 (MQ=255)
                                                             ccAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACa                                                                                     >  1:2355770/1‑150 (MQ=255)
                                                               aGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAg                                                                                   >  2:886629/1‑150 (MQ=255)
                                                                   cgGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTg                                                                               >  2:2744657/1‑150 (MQ=255)
                                                                    ggCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                              >  2:324385/1‑150 (MQ=255)
                                                                      cTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTc                                                                            <  1:3740561/150‑1 (MQ=255)
                                                                         aCCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAc                                                                         >  1:1910832/1‑150 (MQ=255)
                                                                         aCCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAc                                                                         >  2:2738370/1‑150 (MQ=255)
                                                                           ccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                       >  1:549828/1‑150 (MQ=255)
                                                                           ccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                       >  1:3556548/1‑150 (MQ=255)
                                                                             gTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCTGCTGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                                     <  1:1890363/150‑1 (MQ=255)
                                                                              tttCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAg                                                                    <  2:2444557/150‑1 (MQ=255)
                                                                                  tctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggc                                                                >  2:2950964/1‑150 (MQ=255)
                                                                                      gCAATAATCGTGGTTATTTCGGTTTTTTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGAAAAAGGAATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcg                                                            <  1:1014645/150‑1 (MQ=255)
                                                                                      gCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcg                                                            >  2:661799/1‑150 (MQ=255)
                                                                                        aataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                          >  2:3237791/1‑150 (MQ=255)
                                                                                        aataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                          >  2:1338580/1‑150 (MQ=255)
                                                                                          taatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                        <  1:629187/150‑1 (MQ=255)
                                                                                           aatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCTACAAAGGCTTGACGTTAAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCggcatcggcatc                                                       <  1:705228/150‑1 (MQ=255)
                                                                                           aatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCggcatcggcatc                                                       >  1:1917516/1‑150 (MQ=255)
                                                                                             tCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAATGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                     <  1:3646862/150‑1 (MQ=255)
                                                                                             tCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                     >  1:1601505/1‑150 (MQ=255)
                                                                                             tCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                     >  1:788775/1‑150 (MQ=255)
                                                                                              cgtgtttAATGTGGCTTTTTTGTTTTCCAGCTGTGCGGAAAAGCGTTCGACATAGGTTTGATGTTCATAAAATGAAAGCCGCGCCCTGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                    <  1:3497949/145‑1 (MQ=255)
                                                                                              cgcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                    <  2:33842/150‑1 (MQ=255)
                                                                                               gcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttg                                                   <  2:1787747/150‑1 (MQ=255)
                                                                                                cgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgc                                                  >  1:2912608/1‑150 (MQ=255)
                                                                                                  gTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                                <  1:3146888/150‑1 (MQ=255)
                                                                                                   ttAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                               >  1:2385076/1‑150 (MQ=255)
                                                                                                    tAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                              >  2:1914815/1‑150 (MQ=255)
                                                                                                    tAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                              <  2:1089638/150‑1 (MQ=255)
                                                                                                    tAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                              >  2:3656504/1‑150 (MQ=255)
                                                                                                     taTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgc                                             >  2:2243937/2‑150 (MQ=255)
                                                                                                        tCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCTTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCAt                                          <  1:129742/150‑1 (MQ=255)
                                                                                                          ggCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                                        >  2:742189/1‑150 (MQ=255)
                                                                                                          ggCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                                        >  2:3123473/1‑150 (MQ=255)
                                                                                                          ggCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                                        >  1:650758/1‑150 (MQ=255)
                                                                                                          ggCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                                        >  2:3267496/1‑150 (MQ=255)
                                                                                                             tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAacac                                     <  1:2483366/150‑1 (MQ=255)
                                                                                                             tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAacac                                     >  1:867418/1‑150 (MQ=255)
                                                                                                              ttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                                    <  1:1182739/150‑1 (MQ=255)
                                                                                                              ttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                                    <  1:3198752/150‑1 (MQ=255)
                                                                                                               tATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                   <  2:1526905/150‑1 (MQ=255)
                                                                                                                       tCCAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                           <  1:2783175/150‑1 (MQ=255)
                                                                                                                        ccAGCAGTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                          >  2:3137795/1‑150 (MQ=255)
                                                                                                                         cagcagTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAc                         <  2:2120293/150‑1 (MQ=255)
                                                                                                                          agcagTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                        >  2:2044565/1‑150 (MQ=255)
                                                                                                                          agcagTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTAGCCATAACACCTCGCCCGATAcc                        >  2:2044600/1‑150 (MQ=255)
                                                                                                                            cagTGCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCgg                      >  2:1019572/1‑150 (MQ=255)
                                                                                                                                gCGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTa                  <  1:801398/150‑1 (MQ=255)
                                                                                                                                 cGGCAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTAc                 >  1:3325341/1‑150 (MQ=255)
                                                                                                                                    cAAAGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt              <  2:200942/150‑1 (MQ=255)
                                                                                                                                       aGCGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTca           <  2:672718/150‑1 (MQ=255)
                                                                                                                                         cGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca         >  2:686909/1‑150 (MQ=255)
                                                                                                                                         cGTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca         >  2:2594134/1‑150 (MQ=255)
                                                                                                                                          gTTCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCTGATTACGCTTCacac        >  1:122130/1‑150 (MQ=255)
                                                                                                                                          gTTCGACAAAGGCATGACGTTCAGAAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCacac        <  1:2014631/150‑1 (MQ=255)
                                                                                                                                            tCGACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAcgc      >  2:142550/1‑150 (MQ=255)
                                                                                                                                              gACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGa    >  1:1528917/1‑150 (MQ=255)
                                                                                                                                              gACAAAGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGa    <  2:1176461/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATG  >  CP009273/1823655‑1823948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑