Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,836,179 G→A Q72* (CAG→TAG)  ynjH ← DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,836,1790GA99.5% 711.3 / ‑4.6 214Q72* (CAG→TAG) ynjHDUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (105/108);  total (105/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 214 total aligned reads displayed.

CATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGAC  >  CP009273/1836032‑1836325
                                                                                                                                                   |                                                                                                                                                  
caTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGc                                                                                                                                                  <  1:3374369/150‑1 (MQ=255)
  tGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCAt                                                                                                                                                >  1:1095095/1‑150 (MQ=255)
  tGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCAt                                                                                                                                                >  1:1560753/1‑150 (MQ=255)
   gAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCAtt                                                                                                                                               <  2:1825431/150‑1 (MQ=255)
          aGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGcagcag                                                                                                                                        <  2:1881586/150‑1 (MQ=255)
          aGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGcagcag                                                                                                                                        >  1:3398919/1‑150 (MQ=255)
            gcgcTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGcagcagaa                                                                                                                                      <  2:804349/150‑3 (MQ=255)
            gcgcTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGcagcagaa                                                                                                                                      <  2:2781471/150‑3 (MQ=255)
             cgcTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAAGGTTTTGTAATCACACTAGCATTGCAGCAGAAt                                                                                                                                     >  1:101436/1‑150 (MQ=255)
                  gtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCtt                                                                                                                                <  2:1773461/150‑1 (MQ=255)
                  gtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCtt                                                                                                                                <  1:1421429/150‑1 (MQ=255)
                     tggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCgg                                                                                                                             <  2:2455647/150‑1 (MQ=255)
                         gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCttt                                                                                                                         <  1:420812/150‑1 (MQ=255)
                         gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCttt                                                                                                                         <  2:2989646/150‑1 (MQ=255)
                         gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACAGTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCttt                                                                                                                         <  1:420221/150‑1 (MQ=255)
                          cTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTa                                                                                                                        >  2:2179509/1‑150 (MQ=255)
                          cTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTa                                                                                                                        >  2:1916654/1‑150 (MQ=255)
                          cTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTa                                                                                                                        <  2:1134761/150‑1 (MQ=255)
                           tCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                       >  2:1362107/1‑150 (MQ=255)
                           tCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATAGCAATAAGAGCCCCCAGACCAACGGAATATATCTAAAAGTTTTTTCAACACGCTAACAATGCAACAAAATTACTACGGCTTTaa                                                                                                                       >  1:79596/1‑150 (MQ=255)
                            cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGCTTTACGCGCCGCCAGACCAACGGATTAGTGCTAAGGGTTTTGTAATCACGCTAGCATTGCAGCAGAATTACTTCGGCTTTAAt                                                                                                                      >  1:2236437/1‑150 (MQ=255)
                                tGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTAcc                                                                                                                  >  1:1907557/1‑150 (MQ=255)
                                 gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCg                                                                                                                 >  2:493501/1‑150 (MQ=255)
                                 gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCg                                                                                                                 >  2:1126399/1‑150 (MQ=255)
                                  aaGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGc                                                                                                                >  2:408953/1‑150 (MQ=255)
                                  aaGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGc                                                                                                                >  1:774281/1‑150 (MQ=255)
                                    gagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCCGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTAACGccc                                                                                                              >  1:1657010/1‑150 (MQ=255)
                                    gagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGccc                                                                                                              >  2:2535739/1‑150 (MQ=255)
                                     agGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGcccc                                                                                                             >  2:1564150/1‑150 (MQ=255)
                                      ggCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCt                                                                                                            <  2:468571/150‑1 (MQ=255)
                                       gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCtt                                                                                                           <  1:311039/150‑1 (MQ=255)
                                       gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCtt                                                                                                           <  1:2419918/150‑1 (MQ=255)
                                       gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCtt                                                                                                           <  1:3247630/150‑1 (MQ=255)
                                         gcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCa                                                                                                         <  1:3754892/150‑1 (MQ=255)
                                          cTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAg                                                                                                        <  1:3753884/150‑1 (MQ=255)
                                            gCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                                                                      <  1:1183456/150‑1 (MQ=255)
                                                tatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaataat                                                                                                  <  1:3544807/150‑1 (MQ=255)
                                                      cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGAt                                                                                            <  2:2029090/150‑1 (MQ=255)
                                                       tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATc                                                                                           >  1:1248880/1‑150 (MQ=255)
                                                         gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCt                                                                                         <  1:874895/150‑1 (MQ=255)
                                                         gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCt                                                                                         <  1:2044549/150‑1 (MQ=255)
                                                         gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCt                                                                                         <  1:3474854/150‑1 (MQ=255)
                                                          ccccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTg                                                                                        >  2:3660910/1‑150 (MQ=255)
                                                          ccccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTg                                                                                        <  1:2843421/150‑1 (MQ=255)
                                                           cccTGCTGACATTCCATTATTAGAGGCGTTTATTGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAATGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCATCAAAATAATTTTGATCCTGa                                                                                       >  1:706569/1‑150 (MQ=255)
                                                            ccTGCTGACATTCCATTATTAGAGGCGTTTATTGCTTTACGCGCCGCCAGACCAGCGGATTATTGCTAAGGGTTTTGTCATCACGCTAGCAATTCAGCAGAATTACTTCGGCTTTAATTAACGCCCCTTCAGAAAAATTTTTATACTGAt                                                                                      >  1:2880431/1‑150 (MQ=255)
                                                             ctgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATa                                                                                     <  1:1356747/150‑1 (MQ=255)
                                                             ctgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATa                                                                                     >  2:163781/1‑150 (MQ=255)
                                                             ctgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATa                                                                                     <  1:2925777/150‑1 (MQ=255)
                                                              tgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGAATAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaa                                                                                    >  2:2930725/1‑150 (MQ=255)
                                                               gctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaaa                                                                                   >  2:3491589/1‑150 (MQ=255)
                                                                ctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAAc                                                                                  <  2:3120545/150‑1 (MQ=255)
                                                                 tgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACg                                                                                 <  1:1830689/150‑1 (MQ=255)
                                                                  gACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgc                                                                                <  1:522827/150‑1 (MQ=255)
                                                                  gACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgc                                                                                <  1:2180303/150‑1 (MQ=255)
                                                                    cATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcag                                                                              <  1:399469/150‑1 (MQ=255)
                                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcagca                                                                            <  2:639812/150‑1 (MQ=255)
                                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcagca                                                                            <  1:2517248/150‑1 (MQ=255)
                                                                        ccATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACt                                                                          >  2:3046813/1‑150 (MQ=255)
                                                                           ttattaGAGGCGTTTATGGCTTTACGCGCCGCAAGAACAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAg                                                                       <  1:3392613/150‑1 (MQ=255)
                                                                               taGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACa                                                                   <  1:2755619/150‑1 (MQ=255)
                                                                                     cGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTg                                                             >  2:2959409/1‑150 (MQ=255)
                                                                                      gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                                                            >  2:1414877/1‑150 (MQ=255)
                                                                                      gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                                                            <  1:307745/150‑1 (MQ=255)
                                                                                      gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                                                            >  1:974185/1‑150 (MQ=255)
                                                                                         tATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCt                                                         >  2:871021/1‑150 (MQ=255)
                                                                                         tATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCt                                                         >  1:2380883/1‑150 (MQ=255)
                                                                                           tGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                       >  1:212823/1‑150 (MQ=255)
                                                                                            ggCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCGTCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAc                                                      <  2:493841/150‑1 (MQ=255)
                                                                                            ggCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAc                                                      <  2:3290520/150‑1 (MQ=255)
                                                                                                 tACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGccc                                                 >  1:164009/1‑150 (MQ=255)
                                                                                                   cgcgCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGc                                               >  2:2519974/1‑150 (MQ=255)
                                                                                                     cgccgcCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCct                                             <  1:1166554/150‑1 (MQ=255)
                                                                                                      gccgccAGACCAGCGGATTAGTGCTAATGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctg                                            <  2:776082/150‑1 (MQ=255)
                                                                                                        cgccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaa                                          >  1:488745/1‑150 (MQ=255)
                                                                                                           cAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactg                                       >  1:3437365/1‑150 (MQ=255)
                                                                                                             gACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactgaa                                     >  2:1084104/1‑150 (MQ=255)
                                                                                                             gACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGAGCCTGATACACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactgaa                                     >  2:1305306/1‑150 (MQ=255)
                                                                                                              aCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                    >  1:151936/1‑150 (MQ=255)
                                                                                                              aCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                    >  1:1596412/1‑150 (MQ=255)
                                                                                                              aCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                    >  2:2569078/1‑150 (MQ=255)
                                                                                                              aCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                    >  1:3493153/1‑150 (MQ=255)
                                                                                                                cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAacac                                  >  2:2818112/1‑150 (MQ=255)
                                                                                                                 aGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACt                                 >  2:1611147/1‑150 (MQ=255)
                                                                                                                   cGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                               >  2:3596162/1‑150 (MQ=255)
                                                                                                                      aTTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTgg                            >  2:1507955/1‑150 (MQ=255)
                                                                                                                          gTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGt                        <  1:2715749/150‑1 (MQ=255)
                                                                                                                           tGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTa                       >  2:2612706/1‑150 (MQ=255)
                                                                                                                              tAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGt                    >  2:38567/1‑150 (MQ=255)
                                                                                                                               aaGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGtt                   <  2:3388882/150‑1 (MQ=255)
                                                                                                                               aaGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGtt                   >  1:1086447/1‑150 (MQ=255)
                                                                                                                                aGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGACCACTTCTGGCGGTACGttt                  >  2:2756906/1‑150 (MQ=255)
                                                                                                                                   gTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAcc               >  1:3647056/1‑150 (MQ=255)
                                                                                                                                      ttGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTcc            <  2:3705092/150‑1 (MQ=255)
                                                                                                                                       tGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCa           <  1:3283251/150‑1 (MQ=255)
                                                                                                                                          catcaCGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGt        <  1:1362107/150‑1 (MQ=255)
                                                                                                                                           atcaCGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTc       >  1:3151898/1‑150 (MQ=255)
                                                                                                                                             caCGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCg     >  2:3678874/1‑150 (MQ=255)
                                                                                                                                               cGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGa   >  1:3046354/1‑150 (MQ=255)
                                                                                                                                                gCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGAc  >  1:1401776/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGAC  >  CP009273/1836032‑1836325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑