Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,865,836 G→T D399Y (GAT→TAT)  yeaI → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,865,8360GT100.0% 769.1 / NA 229D399Y (GAT→TAT) yeaIputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (134/95);  total (134/95)

Only 100 of 233 total aligned reads displayed.

TTGTCGCGACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAA  >  CP009273/1865689‑1865982
                                                                                                                                                   |                                                                                                                                                  
 tGTCGCGACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATtata                                                                                                                                                 <  1:212600/150‑1 (MQ=255)
    cgcgACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATAttt                                                                                                                                              <  2:1659364/150‑1 (MQ=255)
      cgACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTa                                                                                                                                            >  2:3303004/1‑150 (MQ=255)
       gACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAg                                                                                                                                           >  1:1208064/1‑150 (MQ=255)
         ccTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAgcg                                                                                                                                         >  1:3800519/1‑150 (MQ=255)
           tgttgtTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGcgcg                                                                                                                                       >  2:705524/1‑150 (MQ=255)
             ttgttAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGAc                                                                                                                                     <  1:1973905/150‑1 (MQ=255)
                 tAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGa                                                                                                                                 >  1:2207092/1‑150 (MQ=255)
                        ttCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAg                                                                                                                          >  1:879181/1‑150 (MQ=255)
                         tCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAgg                                                                                                                         >  2:772926/1‑150 (MQ=255)
                            gtgtCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGgtgt                                                                                                                      >  1:169660/1‑150 (MQ=255)
                             tgtCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGtt                                                                                                                     >  1:566388/1‑150 (MQ=255)
                               tCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTg                                                                                                                   <  2:2258575/150‑1 (MQ=255)
                               tCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTg                                                                                                                   >  2:1502921/1‑150 (MQ=255)
                               tCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCAAATGGGGACATCGTGTGGGTGATAAAGTGATGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTg                                                                                                                   >  2:1241379/1‑150 (MQ=255)
                                   gCTGGTTGATATTAATCAGTTCAAACGCATCACTGCCCACTGGGGACATCGGGTGGGTGATAAAGTGCTGGTTTCAAGTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCtt                                                                                                               <  2:2613422/150‑1 (MQ=255)
                                   gCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCtt                                                                                                               <  2:1921722/150‑1 (MQ=255)
                                     tGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGc                                                                                                             <  2:3157208/150‑1 (MQ=255)
                                      ggTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCt                                                                                                            >  1:1510985/1‑150 (MQ=255)
                                       gTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTa                                                                                                           <  2:1366033/150‑1 (MQ=255)
                                       gTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTa                                                                                                           <  1:57679/150‑1 (MQ=255)
                                          gATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTAttt                                                                                                        <  1:990032/150‑1 (MQ=255)
                                           atatTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTa                                                                                                       >  2:2011325/1‑150 (MQ=255)
                                           atatTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTa                                                                                                       >  2:98864/1‑150 (MQ=255)
                                               tAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGa                                                                                                   <  2:1700331/150‑1 (MQ=255)
                                                  tCAGTACAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCAGCCCCGATTATATTTTAGCGAGACTGGAGGGTGAGGTGTTTGGCTTGCTATATACCGAACt                                                                                                >  2:2828962/1‑150 (MQ=255)
                                                     gTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCaa                                                                                             <  2:1320681/150‑1 (MQ=255)
                                                       tCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATa                                                                                           >  1:677194/1‑150 (MQ=255)
                                                        cAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAg                                                                                          <  1:2501473/150‑1 (MQ=255)
                                                          aaCGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTg                                                                                        <  1:109060/150‑1 (MQ=255)
                                                            cGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGcc                                                                                      >  2:2744900/1‑150 (MQ=255)
                                                            cGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGcc                                                                                      >  2:2384992/1‑150 (MQ=255)
                                                            cGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGcc                                                                                      <  2:2082295/150‑1 (MQ=255)
                                                              cATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCa                                                                                    <  1:1324650/150‑1 (MQ=255)
                                                               aTCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAg                                                                                   >  2:1862501/1‑150 (MQ=255)
                                                                 cAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGc                                                                                 >  1:2988518/1‑150 (MQ=255)
                                                                  aaggCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCa                                                                                <  2:2094498/147‑1 (MQ=255)
                                                                  aaTGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCa                                                                                <  2:1584585/150‑1 (MQ=255)
                                                                  aaTGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCa                                                                                >  1:3571736/1‑150 (MQ=255)
                                                                  aaTGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCa                                                                                >  1:1982639/1‑150 (MQ=255)
                                                                    tGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCaag                                                                              <  2:816559/150‑2 (MQ=255)
                                                                        cAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATc                                                                          <  1:2757991/150‑1 (MQ=255)
                                                                             gggACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGc                                                                     >  2:3445531/1‑150 (MQ=255)
                                                                             gggACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGc                                                                     >  1:52229/1‑150 (MQ=255)
                                                                              ggACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCg                                                                    >  2:2669152/1‑150 (MQ=255)
                                                                                  aTCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAAc                                                                >  1:3301505/1‑150 (MQ=255)
                                                                                   tCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACg                                                               >  2:1807075/1‑150 (MQ=255)
                                                                                    cGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGt                                                              >  2:1952964/1‑150 (MQ=255)
                                                                                      tgtgGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTAt                                                            <  2:1675549/150‑1 (MQ=255)
                                                                                       gtgGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATg                                                           <  2:890760/150‑1 (MQ=255)
                                                                                       gtgGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATg                                                           >  1:3034781/1‑150 (MQ=255)
                                                                                         gggTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCg                                                         <  2:3340773/150‑1 (MQ=255)
                                                                                         gggTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCg                                                         <  2:1093666/150‑1 (MQ=255)
                                                                                           gTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTa                                                       <  1:103168/150‑1 (MQ=255)
                                                                                           gTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTa                                                       <  2:566388/150‑1 (MQ=255)
                                                                                            tGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTaa                                                      <  1:2253036/150‑1 (MQ=255)
                                                                                               tAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTaaaaa                                                   >  1:2549077/1‑150 (MQ=255)
                                                                                               tAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTaaaaa                                                   >  2:2596041/1‑150 (MQ=255)
                                                                                                aaaGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGACCGTATGCGTCAAAAt                                                  >  2:3774756/1‑150 (MQ=255)
                                                                                                aaaGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAAt                                                  <  2:3700651/150‑1 (MQ=255)
                                                                                                aaaGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAAt                                                  <  2:2772843/150‑1 (MQ=255)
                                                                                                aaaGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAAt                                                  >  1:2772878/1‑150 (MQ=255)
                                                                                                aaaGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAAt                                                  >  1:1694152/1‑150 (MQ=255)
                                                                                                  aGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGt                                                <  2:203036/150‑1 (MQ=255)
                                                                                                    tGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCg                                              >  2:765714/1‑150 (MQ=255)
                                                                                                       tGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAATAATCATTGCGGAACGTATGCGTAAAAATGTCGAAc                                           <  1:1380137/150‑1 (MQ=255)
                                                                                                         gTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTc                                         >  1:3086509/1‑150 (MQ=255)
                                                                                                            tCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTg                                      <  2:2431551/150‑1 (MQ=255)
                                                                                                             cAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGa                                     <  1:705524/150‑1 (MQ=255)
                                                                                                               aTTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGAcc                                   <  1:1266318/150‑1 (MQ=255)
                                                                                                               aTTGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGAcc                                   >  1:619628/1‑150 (MQ=255)
                                                                                                                ttGTCGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCg                                  >  2:315630/1‑150 (MQ=255)
                                                                                                                    cGATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCtt                              <  1:2669152/150‑1 (MQ=255)
                                                                                                                     gATATTATCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCttt                             >  1:77283/1‑150 (MQ=255)
                                                                                                                        attatCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGt                          <  2:1814140/150‑1 (MQ=255)
                                                                                                                        attatCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGt                          >  2:1890629/1‑150 (MQ=255)
                                                                                                                          tatCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTaa                        >  2:3235043/1‑150 (MQ=255)
                                                                                                                          tatCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTaa                        >  2:918844/1‑150 (MQ=255)
                                                                                                                           atCCAGCAAAGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTagc                       >  1:1871789/1‑148 (MQ=255)
                                                                                                                                  aaaGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATatg                >  2:1984099/1‑150 (MQ=255)
                                                                                                                                  aaaGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATatg                >  1:32309/1‑150 (MQ=255)
                                                                                                                                   aaGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATatga               >  1:1147030/1‑150 (MQ=255)
                                                                                                                                   aaGCATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATatga               >  1:1146518/1‑150 (MQ=255)
                                                                                                                                      cATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGt            <  2:450936/150‑1 (MQ=255)
                                                                                                                                      cATCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGt            <  1:3446572/150‑1 (MQ=255)
                                                                                                                                       aTCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGtt           >  1:916005/1‑150 (MQ=255)
                                                                                                                                       aTCCGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGtt           <  1:2445354/150‑1 (MQ=255)
                                                                                                                                          cGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCt        >  2:1312537/1‑150 (MQ=255)
                                                                                                                                          cGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCt        <  1:2933689/150‑1 (MQ=255)
                                                                                                                                          cGCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCt        <  1:705770/150‑1 (MQ=255)
                                                                                                                                           gCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTg       >  1:3725196/1‑150 (MQ=255)
                                                                                                                                           gCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTg       >  2:2816991/1‑150 (MQ=255)
                                                                                                                                           gCCCCGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTg       >  1:1451228/1‑150 (MQ=255)
                                                                                                                                            ccccGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGa      >  2:587587/1‑150 (MQ=255)
                                                                                                                                             cccGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGaa     >  2:3608499/1‑150 (MQ=255)
                                                                                                                                             cccGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGCTCCTGaa     <  2:1208064/150‑1 (MQ=255)
                                                                                                                                              ccGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAAc    <  2:677194/150‑1 (MQ=255)
                                                                                                                                              ccGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAAc    <  1:1616948/150‑1 (MQ=255)
                                                                                                                                               cGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACa   >  2:2463138/1‑150 (MQ=255)
                                                                                                                                               cGATTATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCAAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTACTGAACa   >  2:1453510/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGTCGCGACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAA  >  CP009273/1865689‑1865982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑