Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,003,722 G→T E141* (GAA→TAA)  yedK → DUF159 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,003,7220GT99.6% 756.8 / ‑4.6 225E141* (GAA→TAA) yedKDUF159 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (135/89);  minor base C (0/1);  total (135/90)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 225 total aligned reads displayed.

CAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAGAAATCGCG  >  CP009273/2003575‑2003868
                                                                                                                                                   |                                                                                                                                                  
cAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTaa                                                                                                                                                  <  2:1720859/150‑1 (MQ=255)
 aaCATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAg                                                                                                                                                 >  1:2034296/1‑150 (MQ=255)
          gggCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATGGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGAt                                                                                                                                        >  2:1403251/1‑150 (MQ=255)
          gggCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGAt                                                                                                                                        >  2:3479826/1‑150 (MQ=255)
                   gTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCg                                                                                                                               >  2:2362817/1‑150 (MQ=255)
                   gTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCg                                                                                                                               >  2:1912732/1‑150 (MQ=255)
                    ttttGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGt                                                                                                                              <  2:557302/150‑1 (MQ=255)
                     tttGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTc                                                                                                                             <  2:3738435/150‑1 (MQ=255)
                       tGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCAc                                                                                                                           >  1:993925/1‑150 (MQ=255)
                        gCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCAct                                                                                                                          <  2:636369/150‑1 (MQ=255)
                        gCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCAct                                                                                                                          <  2:2464841/150‑1 (MQ=255)
                        gCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCAct                                                                                                                          <  2:1483509/150‑1 (MQ=255)
                         ccGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCActg                                                                                                                         >  1:402737/1‑150 (MQ=255)
                            aTGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGCTGTTTGATCGTCActgctg                                                                                                                      >  2:2870949/1‑150 (MQ=255)
                             tGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACtgctgc                                                                                                                     <  1:3309136/150‑1 (MQ=255)
                              ggCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTTTCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCg                                                                                                                    >  1:669530/1‑150 (MQ=255)
                              ggCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCg                                                                                                                    >  2:1230522/1‑150 (MQ=255)
                                 tGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCa                                                                                                                 >  2:2107979/1‑150 (MQ=255)
                                  ggTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAg                                                                                                                >  1:3184101/1‑150 (MQ=255)
                                    tttGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGAt                                                                                                              <  2:809584/150‑1 (MQ=255)
                                         gTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAgg                                                                                                         >  2:2786884/1‑150 (MQ=255)
                                          tGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGt                                                                                                        >  1:1178585/1‑150 (MQ=255)
                                            gAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCt                                                                                                      <  2:394545/150‑1 (MQ=255)
                                              aaaaaGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTgg                                                                                                    <  1:2608224/150‑1 (MQ=255)
                                               aaaaGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGt                                                                                                   <  2:3046775/150‑1 (MQ=255)
                                               aaaaGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGt                                                                                                   >  2:1535694/1‑150 (MQ=255)
                                                aaaGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTa                                                                                                  >  2:1360733/1‑150 (MQ=255)
                                                 cagaagGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTCTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAg                                                                                                 <  2:2209186/149‑1 (MQ=255)
                                                 aagaagGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAg                                                                                                 >  1:1961701/1‑150 (MQ=255)
                                                  agaagGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGa                                                                                                >  1:1390155/1‑150 (MQ=255)
                                                    aagGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGata                                                                                              >  2:2652182/1‑150 (MQ=255)
                                                     agGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGatat                                                                                             <  1:580498/150‑1 (MQ=255)
                                                      ggCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATAtt                                                                                            >  2:3760712/1‑150 (MQ=255)
                                                       gCGACAAACCACAGCCTTTTTTTATCGATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGGGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTc                                                                                           <  2:3215903/150‑1 (MQ=255)
                                                       gCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTc                                                                                           <  2:707733/150‑1 (MQ=255)
                                                         gACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCAt                                                                                         >  1:2334190/1‑150 (MQ=255)
                                                         gACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCAt                                                                                         >  1:3280515/1‑150 (MQ=255)
                                                         gACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCAt                                                                                         >  2:117545/1‑150 (MQ=255)
                                                          aCAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATg                                                                                        >  2:1789078/1‑150 (MQ=255)
                                                           cAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGa                                                                                       >  2:1608208/1‑150 (MQ=255)
                                                             aaaaaCAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGAcc                                                                                     >  1:2425920/1‑150 (MQ=255)
                                                               aaaCAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGAccgc                                                                                   >  2:1416314/1‑150 (MQ=255)
                                                               aaaCAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGAccgc                                                                                   >  2:1768318/1‑150 (MQ=255)
                                                                aaCAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGAccgcc                                                                                  >  1:1937561/1‑150 (MQ=255)
                                                                 aCAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGAccgccg                                                                                 >  2:2079623/1‑150 (MQ=255)
                                                                  cAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACcgccgc                                                                                >  2:2249662/1‑150 (MQ=255)
                                                                    gCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGccc                                                                              <  2:106061/150‑1 (MQ=255)
                                                                     ccTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCa                                                                             <  1:262245/150‑1 (MQ=255)
                                                                     ccTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCa                                                                             <  1:1520270/150‑1 (MQ=255)
                                                                        ttttttATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTg                                                                          >  1:1215663/1‑150 (MQ=255)
                                                                         tttttATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTgg                                                                         >  1:232741/1‑150 (MQ=255)
                                                                          ttttATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGt                                                                        >  1:2445946/1‑150 (MQ=255)
                                                                           tttATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGTGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTa                                                                       >  1:3012922/1‑150 (MQ=255)
                                                                            ttATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTAc                                                                      >  1:1469630/1‑150 (MQ=255)
                                                                              atctatcGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTg                                                                    >  1:909606/1‑150 (MQ=255)
                                                                               tctatcGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGt                                                                   >  2:1473731/1‑150 (MQ=255)
                                                                                  atcGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGc                                                                >  1:27448/1‑150 (MQ=255)
                                                                                    cGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCa                                                              <  1:53652/150‑1 (MQ=255)
                                                                                     gTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAg                                                             >  2:831684/1‑150 (MQ=255)
                                                                                          gATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCACCGCCCACTGGTACTGTCGCCAGAAGCt                                                        <  2:3550732/150‑1 (MQ=255)
                                                                                           aTGGACAGCCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTg                                                       >  2:2533332/1‑150 (MQ=255)
                                                                                                cAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCgg                                                  >  2:258673/1‑150 (MQ=255)
                                                                                                cAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCgg                                                  >  2:916145/1‑150 (MQ=255)
                                                                                                 aaCCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCggg                                                 >  1:2475057/1‑150 (MQ=255)
                                                                                                 aaCCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCggg                                                 >  2:3253187/1‑150 (MQ=255)
                                                                                                   ccTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGaa                                               >  1:239644/1‑150 (MQ=255)
                                                                                                    cTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAAt                                              <  2:3379297/150‑1 (MQ=255)
                                                                                                        ttttATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGAt                                          <  1:725192/150‑1 (MQ=255)
                                                                                                         tttATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATg                                         >  1:1693905/1‑150 (MQ=255)
                                                                                                          ttATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGc                                        <  2:3315346/150‑1 (MQ=255)
                                                                                                             tGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGc                                     >  2:1221224/1‑150 (MQ=255)
                                                                                                                 cgcgATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAaga                                 <  1:2910011/150‑1 (MQ=255)
                                                                                                                   cgATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAgaga                               >  2:1419625/1‑150 (MQ=255)
                                                                                                                    gATAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGAt                              <  1:3280867/150‑1 (MQ=255)
                                                                                                                     aTAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGAtt                             <  1:2104296/150‑1 (MQ=255)
                                                                                                                     aTAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGAtt                             <  1:3668019/150‑1 (MQ=255)
                                                                                                                      tAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTa                            <  2:2330516/150‑1 (MQ=255)
                                                                                                                      tAGGTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTa                            <  2:3181661/150‑1 (MQ=255)
                                                                                                                       aGGTAGCACACCATTTGAGCGTGGTGACTAAGCAGAAGGATATTAGATCGACACTGCTGCGGCAGATCAAGGTCTGGAAGATATTCATTACCGCCGCCCACTGGACCTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAg                           >  2:857589/1‑150 (MQ=255)
                                                                                                                        ggTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGc                          <  1:1230522/150‑1 (MQ=255)
                                                                                                                         gTAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCg                         >  2:660592/1‑150 (MQ=255)
                                                                                                                          tAGCACACCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCgg                        <  1:1475402/150‑1 (MQ=255)
                                                                                                                             cacaCCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTaa                     <  1:3098718/150‑1 (MQ=255)
                                                                                                                             cacaCCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTaa                     <  1:1768318/150‑1 (MQ=255)
                                                                                                                             cacaCCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTaa                     >  1:2189199/1‑150 (MQ=255)
                                                                                                                              acacCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTaaa                    >  1:2663998/1‑150 (MQ=255)
                                                                                                                               cacCATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAaag                   >  2:2973191/1‑150 (MQ=255)
                                                                                                                                 ccATTTGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAaagaa                 >  1:3252369/1‑150 (MQ=255)
                                                                                                                                     ttGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCt             <  1:915248/150‑1 (MQ=255)
                                                                                                                                      tGAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTc            >  2:2921906/1‑150 (MQ=255)
                                                                                                                                       gAGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCa           >  1:1095531/1‑150 (MQ=255)
                                                                                                                                        aGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAg          >  2:3445508/1‑150 (MQ=255)
                                                                                                                                        aGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAg          <  2:1314823/150‑1 (MQ=255)
                                                                                                                                        aGCGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAg          >  1:1744803/1‑150 (MQ=255)
                                                                                                                                          cGTGGTGACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTCCTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAGaa        >  1:1284653/1‑150 (MQ=255)
                                                                                                                                           gtggtgACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAGaaa       >  1:2658506/1‑150 (MQ=255)
                                                                                                                                           gtggtgACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAGaaa       >  1:1947212/1‑150 (MQ=255)
                                                                                                                                            tggtgACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAGAAAt      <  2:3574795/150‑1 (MQ=255)
                                                                                                                                            tggtgACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTAATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAGAAAt      >  1:3467661/1‑150 (MQ=255)
                                                                                                                                               tgACTAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAGAAATcgc   >  2:2137990/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGGTCTGGTAGATATTCATGACCGCCGCCCACTGGTACTGTCGCCAGAAGCTGCGCGGGAATGGATGCGGCAAGAGATTAGCGGTAAAGAAGCCTCAGAAATCGCG  >  CP009273/2003575‑2003868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑