Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,040,890 C→A G824G (GGC→GGA)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,040,8900CA99.5% 670.9 / ‑4.6 200G824G (GGC→GGA) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (105/94);  minor base G (1/0);  total (106/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 200 total aligned reads displayed.

ACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGC  >  CP009273/2040744‑2041035
                                                                                                                                                  |                                                                                                                                                 
aCCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTg                                                                                                                                                >  1:887263/1‑150 (MQ=255)
aCCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTg                                                                                                                                                >  1:3253688/1‑150 (MQ=255)
aCCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTg                                                                                                                                                >  2:828123/1‑150 (MQ=255)
  cGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGaa                                                                                                                                              <  2:441019/150‑1 (MQ=255)
    tCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGaaac                                                                                                                                            >  2:3478216/1‑150 (MQ=255)
        gTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGaaacaaac                                                                                                                                        >  2:869027/1‑150 (MQ=255)
        gTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGaaacaaac                                                                                                                                        <  1:3533635/150‑1 (MQ=255)
           tGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGtt                                                                                                                                     >  1:1219711/1‑150 (MQ=255)
            gCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTa                                                                                                                                    <  2:101900/150‑1 (MQ=255)
                    aaGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAg                                                                                                                            >  1:3430564/1‑150 (MQ=255)
                    aaGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAg                                                                                                                            >  2:1370464/1‑150 (MQ=255)
                        ggATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGtttt                                                                                                                        <  1:1860733/150‑1 (MQ=255)
                        ggATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGtttt                                                                                                                        >  2:2334500/1‑150 (MQ=255)
                         gATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTg                                                                                                                       <  2:3192906/150‑1 (MQ=255)
                             ggCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCgg                                                                                                                   >  1:532013/1‑150 (MQ=255)
                             ggCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCgg                                                                                                                   <  2:2262960/150‑1 (MQ=255)
                               cAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCg                                                                                                                 <  2:1617500/150‑1 (MQ=255)
                                  cttccttcAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACt                                                                                                              <  1:2870968/150‑1 (MQ=255)
                                   ttccttcAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTc                                                                                                             <  2:217836/150‑1 (MQ=255)
                                   ttccttcAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTc                                                                                                             <  2:2547098/150‑1 (MQ=255)
                                    tccttcAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCg                                                                                                            >  1:3408222/1‑150 (MQ=255)
                                        tcAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTa                                                                                                        <  2:3059319/150‑1 (MQ=255)
                                        tcAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTa                                                                                                        <  2:3165632/150‑1 (MQ=255)
                                            caaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgc                                                                                                    >  1:2646143/1‑150 (MQ=255)
                                            caaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgc                                                                                                    <  1:183243/150‑1 (MQ=255)
                                             aaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgcg                                                                                                   >  1:1691319/1‑150 (MQ=255)
                                               tCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCa                                                                                                 >  2:1312167/1‑150 (MQ=255)
                                                 aaaaCACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAgg                                                                                               <  1:2233812/150‑1 (MQ=255)
                                                   aaCACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGtt                                                                                             >  1:3154764/1‑150 (MQ=255)
                                                    acacCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTg                                                                                            <  1:2347294/150‑1 (MQ=255)
                                                           aaaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCa                                                                                     >  2:64575/1‑150 (MQ=255)
                                                           aaaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCa                                                                                     >  2:901096/1‑150 (MQ=255)
                                                            aaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAg                                                                                    >  2:1211354/1‑150 (MQ=255)
                                                            aaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAg                                                                                    <  2:1098594/150‑1 (MQ=255)
                                                            aaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAg                                                                                    >  1:253110/1‑150 (MQ=255)
                                                                cGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGt                                                                                <  2:3118756/150‑1 (MQ=255)
                                                                 ggATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTc                                                                               <  1:3737259/150‑1 (MQ=255)
                                                                 ggATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTc                                                                               >  2:315827/1‑150 (MQ=255)
                                                                 ggATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTc                                                                               >  1:1065307/1‑150 (MQ=255)
                                                                         aTGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATg                                                                       >  1:3059549/1‑150 (MQ=255)
                                                                           ggTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGaa                                                                     <  1:1435615/150‑1 (MQ=255)
                                                                            gTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGacg                                                                    >  2:599002/1‑148 (MQ=255)
                                                                            gTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAg                                                                    <  1:482424/150‑1 (MQ=255)
                                                                            gTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAg                                                                    >  2:945763/1‑150 (MQ=255)
                                                                            gTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAg                                                                    >  2:598778/1‑150 (MQ=255)
                                                                               tGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTgg                                                                 >  2:1497611/1‑150 (MQ=255)
                                                                                ggCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGt                                                                <  1:1167595/150‑1 (MQ=255)
                                                                                ggCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGt                                                                <  1:3069594/150‑1 (MQ=255)
                                                                                  cGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTg                                                              >  1:3147714/1‑150 (MQ=255)
                                                                                   gACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGc                                                             >  1:177684/1‑150 (MQ=255)
                                                                                     cTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTg                                                           >  2:3104837/1‑150 (MQ=255)
                                                                                     cTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTg                                                           <  1:2153413/150‑1 (MQ=255)
                                                                                     cTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTg                                                           <  1:2742353/150‑1 (MQ=255)
                                                                                     cTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTg                                                           <  2:3648330/150‑1 (MQ=255)
                                                                                     cTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTg                                                           <  2:1831121/150‑1 (MQ=255)
                                                                                       tttGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGAc                                                         <  2:738538/150‑1 (MQ=255)
                                                                                        ttGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACg                                                        <  2:747666/150‑1 (MQ=255)
                                                                                            tCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCaa                                                    <  1:349773/150‑1 (MQ=255)
                                                                                              tGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATg                                                  <  1:33017/150‑1 (MQ=255)
                                                                                              tGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATg                                                  >  2:425229/1‑150 (MQ=255)
                                                                                              tGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATg                                                  <  1:828123/150‑1 (MQ=255)
                                                                                              tGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATg                                                  >  2:2773385/1‑150 (MQ=255)
                                                                                               gAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGa                                                 <  2:2837121/150‑1 (MQ=255)
                                                                                                 aaaGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACa                                               <  2:887263/150‑1 (MQ=255)
                                                                                                    gtagtaAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCg                                            >  2:1804623/1‑150 (MQ=255)
                                                                                                     tagtaAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGt                                           >  1:2458113/1‑150 (MQ=255)
                                                                                                      agtaAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTc                                          >  1:3200030/1‑150 (MQ=255)
                                                                                                         aaGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCACTGACAGCGTcaca                                       >  1:645542/1‑150 (MQ=255)
                                                                                                         aaGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTcaca                                       <  2:1935232/150‑1 (MQ=255)
                                                                                                         aaGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTcaca                                       <  2:2237500/150‑1 (MQ=255)
                                                                                                          aGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACaa                                      >  2:967995/1‑150 (MQ=255)
                                                                                                              ggAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGAc                                  >  1:932237/1‑150 (MQ=255)
                                                                                                               gAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGAcc                                 <  1:2074027/150‑1 (MQ=255)
                                                                                                                aaGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACcg                                >  2:2385197/1‑150 (MQ=255)
                                                                                                                  gACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACcgcg                              <  1:1613266/150‑1 (MQ=255)
                                                                                                                  gACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACcgcg                              >  1:2584658/1‑150 (MQ=255)
                                                                                                                   acaacaCGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGa                             >  2:3603806/1‑150 (MQ=255)
                                                                                                                     aacaCGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGAcc                           <  2:2876087/150‑1 (MQ=255)
                                                                                                                     aacaCGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGAcc                           >  2:2760478/1‑150 (MQ=255)
                                                                                                                      acacGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCg                          >  1:3311799/1‑150 (MQ=255)
                                                                                                                       cacGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGGTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGt                         >  2:2650828/1‑150 (MQ=255)
                                                                                                                        acGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTc                        >  2:2464682/1‑150 (MQ=255)
                                                                                                                        acGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTc                        <  1:869027/150‑1 (MQ=255)
                                                                                                                         cGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTcc                       <  2:3794205/150‑1 (MQ=255)
                                                                                                                            ttGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCggg                    <  2:1771090/150‑1 (MQ=255)
                                                                                                                               aaGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATg                 >  2:2971023/1‑150 (MQ=255)
                                                                                                                                aGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGc                >  1:2274353/1‑150 (MQ=255)
                                                                                                                                 gTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCa               <  2:277786/150‑1 (MQ=255)
                                                                                                                                   cACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCaaa             >  2:1458364/1‑150 (MQ=255)
                                                                                                                                     cccTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAg           <  2:1041128/150‑1 (MQ=255)
                                                                                                                                      ccTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAgg          >  1:798937/1‑150 (MQ=255)
                                                                                                                                        ttGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCa        <  1:1396106/150‑1 (MQ=255)
                                                                                                                                         tGAAAATGGGGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCaa       >  2:2008149/1‑150 (MQ=255)
                                                                                                                                           aaaaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAcc     <  2:3505956/150‑1 (MQ=255)
                                                                                                                                           aaaaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAcc     >  2:1866464/1‑150 (MQ=255)
                                                                                                                                            aaaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCt    <  2:3252387/150‑1 (MQ=255)
                                                                                                                                            aaaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCt    <  2:777889/150‑1 (MQ=255)
                                                                                                                                            aaaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCt    >  1:2782140/1‑150 (MQ=255)
                                                                                                                                            aaaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCt    <  2:2411090/150‑1 (MQ=255)
                                                                                                                                             aaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTg   >  2:1417841/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGC  >  CP009273/2040744‑2041035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑