BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,041,346	C→A	I976I (ATC→ATA)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,041,346	0	C	A	99.6%	743.9 / NA	226	I976I (ATC→ATA)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (116/109); minor base T (1/0); total (117/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

ACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAACAG  >  CP009273/2041199‑2041492

aCAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAc                                                                                                                                                  >  1:3169418/1‑150 (MQ=255)

 cAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAcc                                                                                                                                                 <  1:1652773/150‑1 (MQ=255)

  aGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACTTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCt                                                                                                                                                <  1:3201225/150‑1 (MQ=255)

  aGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCt                                                                                                                                                >  1:2679404/1‑150 (MQ=255)

   ggTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCtt                                                                                                                                               <  1:1162365/150‑1 (MQ=255)

     tGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtct                                                                                                                                             <  2:1508797/150‑1 (MQ=255)

     tGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtct                                                                                                                                             <  1:1484880/150‑1 (MQ=255)

      gAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtctc                                                                                                                                            <  2:180159/150‑1 (MQ=255)

        aTTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCtg                                                                                                                                          <  2:2492102/150‑1 (MQ=255)

          tttATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCtgtg                                                                                                                                        <  2:1394301/150‑1 (MQ=255)

             aTCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCa                                                                                                                                     >  2:926378/1‑150 (MQ=255)

                ggTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAac                                                                                                                                  >  2:867970/1‑150 (MQ=255)

                ggTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAac                                                                                                                                  <  2:218116/150‑1 (MQ=255)

                ggTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAac                                                                                                                                  >  1:1180796/1‑150 (MQ=255)

                 gTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacg                                                                                                                                 >  2:2906718/1‑150 (MQ=255)

                   gATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacgac                                                                                                                               >  2:2248795/1‑150 (MQ=255)

                    ataaaaGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacgacg                                                                                                                              <  1:2092950/147‑1 (MQ=255)

                    aTCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacgacg                                                                                                                              <  2:719498/150‑1 (MQ=255)

                      cAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTc                                                                                                                            >  2:3096945/1‑150 (MQ=255)

                      cAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTc                                                                                                                            >  2:3587901/1‑150 (MQ=255)

                       aaaGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCg                                                                                                                           >  2:360640/1‑150 (MQ=255)

                        aaGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGc                                                                                                                          >  1:1855278/1‑150 (MQ=255)

                        aaGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGc                                                                                                                          <  1:3376559/150‑1 (MQ=255)

                         aGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCa                                                                                                                         <  2:1838382/150‑1 (MQ=255)

                         aGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCa                                                                                                                         >  2:1915286/1‑150 (MQ=255)

                            aCTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagt                                                                                                                      <  2:688319/150‑1 (MQ=255)

                            aCTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagt                                                                                                                      <  2:2248407/150‑1 (MQ=255)

                              tgctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagtaa                                                                                                                    <  2:2230117/150‑1 (MQ=255)

                                   cccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtct                                                                                                               <  2:3377266/150‑1 (MQ=255)

                                    ccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtctc                                                                                                              <  2:3190303/150‑1 (MQ=255)

                                    ccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtctc                                                                                                              <  1:49189/150‑1 (MQ=255)

                                       gACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAt                                                                                                           >  2:1260367/1‑150 (MQ=255)

                                        aCCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAtt                                                                                                          >  1:2783301/1‑150 (MQ=255)

                                        aCCCTCAGTGTGCCTTCAGGTGATACCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAtt                                                                                                          >  1:2503484/1‑150 (MQ=255)

                                         cccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTa                                                                                                         >  1:746079/1‑150 (MQ=255)

                                           cTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGc                                                                                                       <  2:1003234/150‑1 (MQ=255)

                                              aGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAAc                                                                                                    >  1:962786/1‑150 (MQ=255)

                                              aGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAAc                                                                                                    <  1:2876300/150‑1 (MQ=255)

                                                 gtgCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgga                                                                                                 <  2:1189767/150‑1 (MQ=255)

                                                  tgCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACggag                                                                                                >  2:2735447/1‑150 (MQ=255)

                                                    ccTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACggagga                                                                                              <  2:1720945/150‑1 (MQ=255)

                                                       tCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGaaaa                                                                                           <  1:580082/150‑1 (MQ=255)

                                                        cAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAg                                                                                          <  2:2779276/150‑1 (MQ=255)

                                                         aGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAgg                                                                                         >  2:2617815/1‑150 (MQ=255)

                                                         aGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAgg                                                                                         >  1:2189403/1‑150 (MQ=255)

                                                         aGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAgg                                                                                         >  1:1397925/1‑150 (MQ=255)

                                                            tGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCAt                                                                                      <  1:820166/150‑1 (MQ=255)

                                                            tGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCAt                                                                                      >  1:2360119/1‑150 (MQ=255)

                                                             gATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATg                                                                                     >  2:9005/1‑150 (MQ=255)

                                                                 tCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGActg                                                                                 <  2:2680138/150‑3 (MQ=255)

                                                                  cACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGa                                                                                >  1:974430/1‑150 (MQ=255)

                                                                    ccGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATa                                                                              >  1:1040079/1‑150 (MQ=255)

                                                                       tCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                           <  2:3337566/150‑1 (MQ=255)

                                                                       tCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                           <  1:393148/150‑1 (MQ=255)

                                                                        cACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTaa                                                                          >  2:1281452/1‑150 (MQ=255)

                                                                         aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAAc                                                                         <  2:2877974/150‑1 (MQ=255)

                                                                         aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAAc                                                                         >  2:262842/1‑150 (MQ=255)

                                                                         aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGAT‑AAGAAATTACCGTCGCTGGTCCAAACGAAGTAGCAAGGAAGTTCAGGTTTAGCAACGGAGGCAAAGGCATGTCGGGTAGTAACg                                                                        >  2:3388974/1‑150 (MQ=255)

                                                                          ccAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCACGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACg                                                                        >  2:1852118/1‑150 (MQ=255)

                                                                          ccAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACg                                                                        >  2:2014407/1‑150 (MQ=255)

                                                                            aaCACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACggg                                                                      <  1:2351243/150‑1 (MQ=255)

                                                                                caGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTg                                                                  <  1:385665/150‑1 (MQ=255)

                                                                                    tCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAAt                                                              <  2:1545129/150‑1 (MQ=255)

                                                                                      cGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCg                                                            >  2:2052870/1‑150 (MQ=255)

                                                                                         aaTATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCt                                                         >  2:1047686/1‑150 (MQ=255)

                                                                                             tatGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCt                                                     >  2:1409583/1‑150 (MQ=255)

                                                                                             tatGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCt                                                     <  2:2153877/150‑1 (MQ=255)

                                                                                              atGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTg                                                    >  2:794235/1‑150 (MQ=255)

                                                                                                gACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGAc                                                  <  1:1816438/150‑1 (MQ=255)

                                                                                                   tGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCgg                                               <  2:2871244/150‑1 (MQ=255)

                                                                                                      aaCCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCAc                                            >  1:1685955/1‑150 (MQ=255)

                                                                                                        ccTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGt                                          >  2:2164841/1‑150 (MQ=255)

                                                                                                           tGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAg                                       >  2:1070798/1‑150 (MQ=255)

                                                                                                              aGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAANTAAGTTCTCGATNAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCgg                                    <  1:1732303/150‑1 (MQ=255)

                                                                                                               ggATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCggg                                   <  2:68637/150‑1 (MQ=255)

                                                                                                                gATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGc                                  <  1:926378/150‑1 (MQ=255)

                                                                                                                   aaaaaTTGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACg                               >  1:894809/1‑150 (MQ=255)

                                                                                                                     aaaTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCa                             >  1:2736447/1‑150 (MQ=255)

                                                                                                                      aaTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCat                            <  1:2980353/150‑1 (MQ=255)

                                                                                                                         ggCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATg                         >  1:2002671/1‑150 (MQ=255)

                                                                                                                           cAACCCACTAAAAGATAAAAAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGAt                       >  2:2835533/1‑150 (MQ=255)

                                                                                                                            aaCCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATc                      >  2:2050016/1‑150 (MQ=255)

                                                                                                                            aaCCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATc                      <  1:1915286/150‑1 (MQ=255)

                                                                                                                              cccACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCAt                    <  2:2280682/150‑1 (MQ=255)

                                                                                                                              cccACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCAt                    <  1:1260367/150‑1 (MQ=255)

                                                                                                                               cnACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATg                   >  1:523245/1‑150 (MQ=255)

                                                                                                                                cACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATgg                  >  1:303401/1‑150 (MQ=255)

                                                                                                                                  cTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCt                >  2:157808/1‑150 (MQ=255)

                                                                                                                                   tAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTc               >  1:3621669/1‑150 (MQ=255)

                                                                                                                                    aaaaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCg              >  1:309991/1‑150 (MQ=255)

                                                                                                                                     aaaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt             >  1:842478/1‑150 (MQ=255)

                                                                                                                                     aaaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt             <  1:2617815/150‑1 (MQ=255)

                                                                                                                                     aaaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt             >  1:3756828/1‑150 (MQ=255)

                                                                                                                                      aaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTc            >  1:3558502/1‑150 (MQ=255)

                                                                                                                                      aaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTc            >  1:1298410/1‑150 (MQ=255)

                                                                                                                                         aTAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg         >  1:158920/1‑150 (MQ=255)

                                                                                                                                            aaGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTa      <  2:3738818/150‑1 (MQ=255)

                                                                                                                                             aGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTaa     >  2:235704/1‑150 (MQ=255)

                                                                                                                                              gAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAAc    >  2:3787287/1‑150 (MQ=255)

                                                                                                                                               aaaTAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAACa   >  2:1817771/1‑150 (MQ=255)

ACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAACAG  >  CP009273/2041199‑2041492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`