BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,043,643	T→A	L1742Q (CTG→CAG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,043,643	0	T	A	99.5%	669.5 / NA	204	L1742Q (CTG→CAG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (94/109); total (94/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

CGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGT  >  CP009273/2043497‑2043789

    aaCCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGAt                                                                                                                                             <  1:2938827/150‑1 (MQ=255)

    aaCCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGAt                                                                                                                                             >  1:2058684/1‑150 (MQ=255)

       ccGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATaaa                                                                                                                                          <  1:3275766/150‑1 (MQ=255)

        cGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAAc                                                                                                                                         >  1:2079892/1‑150 (MQ=255)

         ggTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAAcc                                                                                                                                        <  1:562554/150‑1 (MQ=255)

           tAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTa                                                                                                                                      <  2:2691906/150‑1 (MQ=255)

           tAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTa                                                                                                                                      <  2:3644018/150‑1 (MQ=255)

             gAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACt                                                                                                                                    <  1:2386662/150‑1 (MQ=255)

             gAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACt                                                                                                                                    >  1:909241/1‑150 (MQ=255)

              aaGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTg                                                                                                                                   <  2:1002267/150‑1 (MQ=255)

               aGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGa                                                                                                                                  >  1:3473858/1‑150 (MQ=255)

               aGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGa                                                                                                                                  >  2:1720656/1‑150 (MQ=255)

               aGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGa                                                                                                                                  >  1:1574690/1‑150 (MQ=255)

                ggCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGaa                                                                                                                                 <  2:2992687/150‑1 (MQ=255)

                 gCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAg                                                                                                                                <  1:1981370/150‑1 (MQ=255)

                 gCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAg                                                                                                                                <  2:33215/150‑1 (MQ=255)

                   aTAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTc                                                                                                                              >  2:2531906/1‑150 (MQ=255)

                    tAAAAGTTAATGTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCa                                                                                                                             <  2:2441147/150‑1 (MQ=255)

                      aaaGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAtc                                                                                                                           >  1:3754718/1‑150 (MQ=255)

                       aaGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAtct                                                                                                                          <  1:989452/150‑1 (MQ=255)

                        aGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAtctc                                                                                                                         <  2:2511840/150‑1 (MQ=255)

                        aGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCAtctc                                                                                                                         >  2:2408201/1‑150 (MQ=255)

                         gTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTcg                                                                                                                        >  1:3442608/1‑150 (MQ=255)

                            aaTTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGa                                                                                                                     <  1:1702004/150‑1 (MQ=255)

                            aaTTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGa                                                                                                                     >  1:517394/1‑150 (MQ=255)

                            aaTTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGa                                                                                                                     >  2:2736569/1‑150 (MQ=255)

                                  cgcgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTg                                                                                                               >  2:2081966/1‑150 (MQ=255)

                                    cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGaa                                                                                                             >  2:2777545/1‑150 (MQ=255)

                                    cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGaa                                                                                                             >  2:392373/1‑150 (MQ=255)

                                    cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGaa                                                                                                             >  2:1367791/1‑150 (MQ=255)

                                       aaCCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGc                                                                                                          >  2:2184872/1‑150 (MQ=255)

                                       aaCCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGc                                                                                                          >  2:830844/1‑150 (MQ=255)

                                           tCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGt                                                                                                      <  1:229972/150‑1 (MQ=255)

                                            ccGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTg                                                                                                     <  1:1697840/150‑1 (MQ=255)

                                            ccGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTg                                                                                                     <  1:3752700/150‑1 (MQ=255)

                                              gTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCa                                                                                                   >  2:33958/1‑150 (MQ=255)

                                              gTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCa                                                                                                   >  1:1755487/1‑150 (MQ=255)

                                                 aCGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGAt                                                                                                <  2:3730120/150‑1 (MQ=255)

                                                 aCGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGAt                                                                                                >  1:1900523/1‑150 (MQ=255)

                                                    cTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGtt                                                                                             <  1:3444673/150‑1 (MQ=255)

                                                        gcagcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAAtt                                                                                         <  1:44116/150‑1 (MQ=255)

                                                          agcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCt                                                                                       <  2:770287/150‑1 (MQ=255)

                                                           gcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGTATGCCAGTGCAGATGTTAATTCTg                                                                                      <  2:543755/150‑1 (MQ=255)

                                                           gcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTg                                                                                      <  2:1753433/150‑1 (MQ=255)

                                                            caCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATACCAGTGCAGATGTTAATTCTGc                                                                                     >  1:3662588/1‑150 (MQ=255)

                                                             aCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcg                                                                                    >  1:3726336/1‑150 (MQ=255)

                                                              ccAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcga                                                                                   <  1:968350/150‑1 (MQ=255)

                                                               cAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgac                                                                                  <  1:696446/150‑1 (MQ=255)

                                                               cAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgac                                                                                  <  1:2306110/150‑1 (MQ=255)

                                                                aGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgacg                                                                                 >  1:1005844/1‑150 (MQ=255)

                                                                aGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGT‑CAGATGTTAATTCTGcgacga                                                                                >  2:3460109/1‑150 (MQ=255)

                                                                  cGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAt                                                                               <  1:2138533/150‑1 (MQ=255)

                                                                  cGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAt                                                                               >  2:1529732/1‑150 (MQ=255)

                                                                   gTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAtt                                                                              >  2:3081087/1‑150 (MQ=255)

                                                                    ttGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTa                                                                             <  2:489180/150‑1 (MQ=255)

                                                                         aCGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGt                                                                        <  1:1607518/150‑1 (MQ=255)

                                                                          cGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTc                                                                       >  1:952470/1‑150 (MQ=255)

                                                                                atCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGgaga                                                                 <  2:2703615/150‑1 (MQ=255)

                                                                                  cGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATa                                                               <  2:1358440/150‑1 (MQ=255)

                                                                                  cGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATa                                                               >  2:2262713/1‑150 (MQ=255)

                                                                                  cGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATa                                                               <  1:3555761/150‑1 (MQ=255)

                                                                                   ggggTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATAc                                                              <  2:623363/150‑1 (MQ=255)

                                                                                    gggTTTCGCTGAAATTCTTGTGACAAGCACAGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATAcc                                                             <  1:2875741/150‑1 (MQ=255)

                                                                                            cTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTc                                                     >  1:1066833/1‑150 (MQ=255)

                                                                                             tGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCa                                                    <  2:1255775/150‑1 (MQ=255)

                                                                                             tGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCa                                                    >  1:3088807/1‑150 (MQ=255)

                                                                                             tGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCa                                                    >  1:619503/1‑150 (MQ=255)

                                                                                               aaaTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAgg                                                  >  1:2320365/1‑150 (MQ=255)

                                                                                                 aTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTa                                                >  2:1446782/1‑150 (MQ=255)

                                                                                                            aaGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGcaca                                     >  2:824691/1‑150 (MQ=255)

                                                                                                            aaGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGcaca                                     <  1:266875/150‑1 (MQ=255)

                                                                                                               cACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGa                                  >  2:203500/1‑150 (MQ=255)

                                                                                                                aCCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAc                                 >  1:1467669/1‑150 (MQ=255)

                                                                                                                aCCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAc                                 <  1:3127637/150‑1 (MQ=255)

                                                                                                                aCCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAc                                 >  1:382218/1‑150 (MQ=255)

                                                                                                                 ccGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACg                                <  1:937990/150‑1 (MQ=255)

                                                                                                                   gAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTa                              <  1:2736747/150‑1 (MQ=255)

                                                                                                                    aGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTNg                             <  1:392373/150‑1 (MQ=255)

                                                                                                                       tCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAg                          >  1:2639999/1‑150 (MQ=255)

                                                                                                                         ggACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGtt                        <  2:3659763/150‑1 (MQ=255)

                                                                                                                         ggACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGtt                        >  1:2953480/1‑150 (MQ=255)

                                                                                                                         ggACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGtt                        >  2:1665195/1‑150 (MQ=255)

                                                                                                                          gACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTa                       <  1:76056/150‑1 (MQ=255)

                                                                                                                          gACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTa                       <  2:680451/150‑1 (MQ=255)

                                                                                                                          gACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTa                       <  2:3442608/150‑1 (MQ=255)

                                                                                                                           aCTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTaa                      <  2:3486832/150‑1 (MQ=255)

                                                                                                                             tGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAg                    >  2:2027887/1‑150 (MQ=255)

                                                                                                                             tGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAg                    <  2:3236518/150‑1 (MQ=255)

                                                                                                                                aaaCAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTc                 >  2:1761049/1‑150 (MQ=255)

                                                                                                                                aaaCAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTc                 >  2:918869/1‑150 (MQ=255)

                                                                                                                                  aCAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCAc               <  1:2530054/150‑1 (MQ=255)

                                                                                                                                   cAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACg              <  1:3236942/150‑1 (MQ=255)

                                                                                                                                   cAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACg              <  1:1629162/150‑1 (MQ=255)

                                                                                                                                     gTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGtt            <  1:2777545/150‑1 (MQ=255)

                                                                                                                                     gTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGtt            <  2:3053810/150‑1 (MQ=255)

                                                                                                                                      tttCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTa           <  1:1139275/150‑1 (MQ=255)

                                                                                                                                        tCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAAc         <  2:2657982/150‑1 (MQ=255)

                                                                                                                                           gCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGAc      >  2:2939378/1‑150 (MQ=255)

                                                                                                                                            ccTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGACAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGAcc     >  2:2007024/1‑150 (MQ=255)

                                                                                                                                              tctcaGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAg   >  2:674107/1‑150 (MQ=255)

CGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGT  >  CP009273/2043497‑2043789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`