BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,146,333	C→A	D93Y (GAT→TAT)	yegL ←	VMA domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,146,333	0	C	A	99.6%	714.9 / NA	221	D93Y (GAT→TAT)	yegL	VMA domain protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (118/102); minor base T (0/1); total (118/103)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

CTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCA  >  CP009273/2146186‑2146479

cTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGc                                                                                                                                                  >  2:2166933/1‑150 (MQ=255)

 tGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGcc                                                                                                                                                 >  2:432222/1‑150 (MQ=255)

 tGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGcc                                                                                                                                                 <  2:3458700/150‑1 (MQ=255)

 tGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGcc                                                                                                                                                 >  1:2183328/1‑150 (MQ=255)

    aGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTg                                                                                                                                              >  2:1678457/1‑150 (MQ=255)

    aGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTg                                                                                                                                              >  1:2076557/1‑150 (MQ=255)

      ccTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTggg                                                                                                                                            <  1:2764026/150‑1 (MQ=255)

       cTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGc                                                                                                                                           >  1:96625/1‑150 (MQ=255)

       cTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGc                                                                                                                                           >  1:224109/1‑150 (MQ=255)

        tGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCa                                                                                                                                          <  1:120325/150‑1 (MQ=255)

           cACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAAc                                                                                                                                       >  1:2539774/1‑150 (MQ=255)

             cTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAg                                                                                                                                     <  1:3467238/150‑1 (MQ=255)

              tCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAgg                                                                                                                                    >  1:2345322/1‑150 (MQ=255)

                     ttGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCg                                                                                                                             <  1:2013220/150‑1 (MQ=255)

                               aTCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAAtt                                                                                                                   >  1:1090842/1‑150 (MQ=255)

                                tCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTa                                                                                                                  <  1:2676096/150‑1 (MQ=255)

                                  ggTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgc                                                                                                                >  2:2034197/1‑150 (MQ=255)

                                  ggTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgc                                                                                                                >  1:1879195/1‑150 (MQ=255)

                                  ggTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgc                                                                                                                >  2:3344590/1‑150 (MQ=255)

                                  ggTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgc                                                                                                                <  1:144901/150‑1 (MQ=255)

                                     aaTCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgcggc                                                                                                             >  1:2883911/1‑150 (MQ=255)

                                     aaTCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGATCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCACACAGGATGGGCGGAAAAAAATTAgcggc                                                                                                             >  1:3223810/1‑150 (MQ=255)

                                      aTCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgcggcg                                                                                                            >  2:760391/1‑150 (MQ=255)

                                        cAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCt                                                                                                          <  2:2496562/150‑1 (MQ=255)

                                          ggAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTgg                                                                                                        >  1:458734/1‑150 (MQ=255)

                                          ggAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTgg                                                                                                        >  2:1695351/1‑150 (MQ=255)

                                            aaaaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGAGGGGCGGAACTAAATTAGCGGCGCTGGtc                                                                                                      >  2:3042247/1‑149 (MQ=255)

                                            aaaaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGATTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTa                                                                                                      <  1:2648664/150‑1 (MQ=255)

                                             aaaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTaa                                                                                                     <  2:768271/150‑1 (MQ=255)

                                              aaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTaaa                                                                                                    <  2:2657779/150‑1 (MQ=255)

                                              aaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTaaa                                                                                                    <  2:2112284/150‑1 (MQ=255)

                                              aaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATCAGCGGCGCTGGTaaa                                                                                                    <  1:2995448/150‑1 (MQ=255)

                                               aTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAg                                                                                                   >  1:427629/1‑150 (MQ=255)

                                                  cACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGG‑AAAAAATTAGCGGCGCTGGTAAAGGgtt                                                                                               >  1:2026356/1‑150 (MQ=255)

                                                   aCGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGgtt                                                                                               <  2:3067361/150‑1 (MQ=255)

                                                     gggCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGgttgt                                                                                             <  2:2286427/150‑1 (MQ=255)

                                                      ggCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGgttgtt                                                                                            <  2:1118077/150‑1 (MQ=255)

                                                        cGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTcc                                                                                          >  1:1136308/1‑150 (MQ=255)

                                                        cGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTcc                                                                                          >  2:3609035/1‑150 (MQ=255)

                                                        cGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTcc                                                                                          >  1:2921919/1‑150 (MQ=255)

                                                         gATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCca                                                                                         >  1:301493/1‑150 (MQ=255)

                                                         gATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCca                                                                                         >  1:3509100/1‑150 (MQ=255)

                                                          ataataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCcac                                                                                        >  2:784517/1‑150 (MQ=255)

                                                          ataataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCcac                                                                                        >  1:334007/1‑150 (MQ=255)

                                                           taataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCcaca                                                                                       >  1:283715/1‑150 (MQ=255)

                                                            aataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACAt                                                                                      >  1:2336213/1‑150 (MQ=255)

                                                             ataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATg                                                                                     >  2:910142/1‑150 (MQ=255)

                                                              taCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGc                                                                                    >  2:565346/1‑150 (MQ=255)

                                                              taCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGc                                                                                    >  1:1954266/1‑150 (MQ=255)

                                                               aCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCa                                                                                   >  2:1587148/1‑150 (MQ=255)

                                                                  aaaTACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCg                                                                                >  1:2004642/1‑150 (MQ=255)

                                                                  aaaTACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCg                                                                                <  1:2844972/150‑1 (MQ=255)

                                                                      aCCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGcc                                                                            >  2:1263253/1‑150 (MQ=255)

                                                                         aTTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTNTATAGCCCTGGGNAAACAGGANGGGCGGAAAAAAATTAGCGGCGCTGGNAAAGGGTTGTTCNACATGCACCGGGCCGaa                                                                         >  1:657839/1‑150 (MQ=255)

                                                                         aTTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTCAAGGGTTGTTCCACATGCACCGGGCCGaa                                                                         >  1:2276081/1‑150 (MQ=255)

                                                                         aTTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGaa                                                                         >  2:525383/1‑150 (MQ=255)

                                                                          tttGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAAc                                                                        <  1:2944188/150‑1 (MQ=255)

                                                                           ttGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACg                                                                       <  1:923777/150‑1 (MQ=255)

                                                                           ttGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACg                                                                       <  2:927699/150‑1 (MQ=255)

                                                                           ttGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACg                                                                       <  1:2855477/150‑1 (MQ=255)

                                                                              cACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTca                                                                    <  1:1388494/150‑1 (MQ=255)

                                                                                cGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTcaca                                                                  >  1:621217/1‑150 (MQ=255)

                                                                                 gATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACaa                                                                 >  2:3002629/1‑150 (MQ=255)

                                                                                  aTACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAAt                                                                <  1:2954411/150‑1 (MQ=255)

                                                                                  aTACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAAGGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAAt                                                                <  1:82054/150‑1 (MQ=255)

                                                                                    aCTCATGTTTCCGCTCCTCTACCATATCCAGGGATTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGc                                                              <  1:3256852/150‑1 (MQ=255)

                                                                                     cTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGcc                                                             >  1:779083/1‑150 (MQ=255)

                                                                                         cGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGt                                                         <  2:933115/150‑1 (MQ=255)

                                                                                          gTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGtt                                                        >  2:1881171/1‑150 (MQ=255)

                                                                                          gTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGtt                                                        >  1:2608257/1‑150 (MQ=255)

                                                                                          gTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGtt                                                        <  2:2708391/150‑1 (MQ=255)

                                                                                           tttCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTc                                                       >  1:3134478/1‑150 (MQ=255)

                                                                                            ttCCGCTCCTCTACCATATCCTGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCACACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTcc                                                      <  2:2903813/150‑1 (MQ=255)

                                                                                             tCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCa                                                     <  2:2442062/150‑1 (MQ=255)

                                                                                                   cctcTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCttt                                               <  1:3618944/150‑1 (MQ=255)

                                                                                                     tctACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACTGGTTGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCttttt                                             <  1:1020277/150‑1 (MQ=255)

                                                                                                     tctACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCttttt                                             <  1:243925/150‑1 (MQ=255)

                                                                                                        aCCATATCTAGGGATTTGGTAATGGCTGTACCCTTGGGTGTATAGCCCTGGGCAAACTGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTaaa                                          <  1:1675778/150‑1 (MQ=255)

                                                                                                        aCCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTaaa                                          >  1:1324506/1‑150 (MQ=255)

                                                                                                         ccATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAg                                         >  2:3559296/1‑150 (MQ=255)

                                                                                                          cATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGc                                        <  1:1767443/150‑1 (MQ=255)

                                                                                                            tatCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCa                                      <  1:3546041/150‑1 (MQ=255)

                                                                                                                 aGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGa                                 >  2:744989/1‑150 (MQ=255)

                                                                                                                 aGGGCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGa                                 >  1:928376/1‑150 (MQ=255)

                                                                                                                   ggCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATc                               >  1:1556402/1‑150 (MQ=255)

                                                                                                                    gCTTTGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCa                              >  1:249887/1‑150 (MQ=255)

                                                                                                                        tGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCagcaa                          <  1:310293/150‑1 (MQ=255)

                                                                                                                        tGGTAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCagcaa                          <  2:1793377/150‑1 (MQ=255)

                                                                                                                           tAATGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACCCTTTTTAAAGCCAGCGGATCagcaagca                       <  1:404009/150‑1 (MQ=255)

                                                                                                                            aaTGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAg                      >  2:1259793/1‑150 (MQ=255)

                                                                                                                              tGGCCGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGtt                    >  1:2727725/1‑150 (MQ=255)

                                                                                                                                 ccGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTtcat                 <  1:2166933/150‑1 (MQ=255)

                                                                                                                                  cGCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTtcatc                >  2:1517377/1‑150 (MQ=255)

                                                                                                                                   gCACCCATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTtcatca               >  1:728101/1‑150 (MQ=255)

                                                                                                                                        cATTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGaaa          <  1:1277955/150‑1 (MQ=255)

                                                                                                                                         aTTGGTGTATAGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAg         >  2:3235727/1‑150 (MQ=255)

                                                                                                                                              tgtATAGCACTGGGCAAACAGGATGGGCTGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGTGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAAc    <  2:1465019/150‑1 (MQ=255)

                                                                                                                                                tataGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCa  <  2:2539774/150‑1 (MQ=255)

                                                                                                                                                tataGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCa  >  2:1097024/1‑150 (MQ=255)

                                                                                                                                                tataGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCa  <  2:2072389/150‑1 (MQ=255)

CTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCA  >  CP009273/2146186‑2146479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`