Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,183,642 C→A D250Y (GAT→TAT)  yehB ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,183,6420CA99.6% 877.5 / NA 263D250Y (GAT→TAT) yehBputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (149/113);  minor base T (0/1);  total (149/114)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

AATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATCACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTA  >  CP009273/2183495‑2183787
                                                                                                                                                   |                                                                                                                                                 
aaTCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAAc                                                                                                                                                 >  1:1155234/1‑150 (MQ=255)
aaTCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAAc                                                                                                                                                 <  1:1977742/150‑1 (MQ=255)
    tGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGaaa                                                                                                                                             >  1:2217740/1‑150 (MQ=255)
      tAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAaaca                                                                                                                                           <  2:3470932/150‑1 (MQ=255)
       aaTCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAaacaa                                                                                                                                          >  1:3338392/1‑150 (MQ=255)
         tCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAAcc                                                                                                                                        <  1:1550270/150‑1 (MQ=255)
            cgAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGca                                                                                                                                     >  1:1795877/1‑150 (MQ=255)
              aaCGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCATCGCGTTACTCTTAGCAATCCCCTGCAACCGTGGCGTAAAATTTTTTTTCGAGTTAGGCAACATCTGCATATAACTAAACAACCTcaca                                                                                                                                   >  1:3423394/1‑150 (MQ=255)
              aaCGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGcaca                                                                                                                                   >  2:1571885/1‑150 (MQ=255)
                 gggCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACct                                                                                                                                <  2:3026883/150‑1 (MQ=255)
                   gCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACctct                                                                                                                              >  1:3612824/1‑150 (MQ=255)
                   gCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACctct                                                                                                                              <  2:806297/150‑1 (MQ=255)
                    ccAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTg                                                                                                                             >  2:2403316/1‑150 (MQ=255)
                    ccAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTg                                                                                                                             >  2:3176087/1‑150 (MQ=255)
                     cAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGa                                                                                                                            >  1:1730768/1‑150 (MQ=255)
                         aggaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCGGAGCAATCCCCTGCACCCGTGGCGTCCAATTTTGTTTCGAGTTAGGCACCATCGGCATATAACGAAACAACCGCACGCCTCTGAcgcg                                                                                                                        >  2:3252829/1‑150 (MQ=255)
                          ggaACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGa                                                                                                                       >  2:127258/1‑150 (MQ=255)
                             aCCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACa                                                                                                                    >  1:210548/1‑150 (MQ=255)
                             aCCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACa                                                                                                                    >  2:3581755/1‑150 (MQ=255)
                                  tttCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATc                                                                                                               <  1:3739522/150‑1 (MQ=255)
                                        aTAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAat                                                                                                         >  1:2482266/1‑150 (MQ=255)
                                        aTAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAat                                                                                                         >  1:3573741/1‑150 (MQ=255)
                                        aTAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAat                                                                                                         >  1:861477/1‑150 (MQ=255)
                                         tAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAata                                                                                                        >  2:1436668/1‑150 (MQ=255)
                                           aaCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAatata                                                                                                      >  2:2880629/1‑149 (MQ=255)
                                           aaCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATc                                                                                                      >  2:1052199/1‑150 (MQ=255)
                                           aaCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATc                                                                                                      <  1:2985599/150‑1 (MQ=255)
                                           aaCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATc                                                                                                      <  2:848334/150‑1 (MQ=255)
                                            aCCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCg                                                                                                     >  2:3143602/1‑150 (MQ=255)
                                              cacaAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCt                                                                                                   >  2:603672/1‑150 (MQ=255)
                                               acaAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACATAAACAAAAATAACGCtt                                                                                                  >  1:3482240/1‑150 (MQ=255)
                                                  aaaCTATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGATACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAt                                                                                               <  2:2809087/150‑1 (MQ=255)
                                                  aaaCCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAt                                                                                               <  1:672527/150‑1 (MQ=255)
                                                  aaaCCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAt                                                                                               >  1:1737851/1‑150 (MQ=255)
                                                  aaaCCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAt                                                                                               >  1:1040571/1‑150 (MQ=255)
                                                     ccATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAtgtg                                                                                            >  1:355282/1‑150 (MQ=255)
                                                     ccATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAtgtg                                                                                            <  2:1251145/150‑1 (MQ=255)
                                                     ccATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGAtgtg                                                                                            <  1:3689342/150‑1 (MQ=255)
                                                        ttCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTAc                                                                                         >  1:3085626/1‑150 (MQ=255)
                                                           tGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCGCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACata                                                                                      >  1:1676319/1‑150 (MQ=255)
                                                            gTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACatat                                                                                     <  2:453726/150‑1 (MQ=255)
                                                            gTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACatat                                                                                     >  2:1029500/1‑150 (MQ=255)
                                                            gTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACatat                                                                                     >  2:975232/1‑150 (MQ=255)
                                                               cAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCAc                                                                                  <  1:3065608/150‑1 (MQ=255)
                                                                 aTAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCAccc                                                                                >  1:3673663/1‑150 (MQ=255)
                                                                  tAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCa                                                                               >  2:641405/1‑150 (MQ=255)
                                                                   aGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCAc                                                                              >  2:3553403/1‑150 (MQ=255)
                                                                         cAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAg                                                                        >  2:3768746/1‑150 (MQ=255)
                                                                         cAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAg                                                                        <  2:517040/150‑1 (MQ=255)
                                                                          aGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGc                                                                       <  2:216037/150‑1 (MQ=255)
                                                                          aGCGCGTTACTCTGAGCAATCCCCTGCACCCGAGGCGTAACATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATAACGCTTGATGTGTACATATCACCCACGCGAAGc                                                                       <  1:726391/150‑1 (MQ=255)
                                                                           gcgcGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCg                                                                      <  2:1956384/150‑1 (MQ=255)
                                                                              cgTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGc                                                                   >  2:2661195/1‑150 (MQ=255)
                                                                              cgTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGc                                                                   >  2:707329/1‑150 (MQ=255)
                                                                                ttACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCg                                                                 >  2:1865552/1‑150 (MQ=255)
                                                                                 tACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCga                                                                <  1:62281/150‑1 (MQ=255)
                                                                                  aCTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCgag                                                               >  1:2670964/1‑150 (MQ=255)
                                                                                  aCTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCgag                                                               >  1:404203/1‑150 (MQ=255)
                                                                                  aCTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCgag                                                               >  1:2670615/1‑150 (MQ=255)
                                                                                    tctGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGaa                                                             <  2:1093397/150‑1 (MQ=255)
                                                                                     ctGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAg                                                            >  2:248427/1‑150 (MQ=255)
                                                                                           aaTCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGc                                                      >  2:3006034/1‑150 (MQ=255)
                                                                                           aaTCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGc                                                      >  2:169118/1‑150 (MQ=255)
                                                                                            aTCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCa                                                     >  2:3051411/1‑150 (MQ=255)
                                                                                               cccTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAAt                                                  >  1:131260/1‑150 (MQ=255)
                                                                                               cccTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAAt                                                  >  1:660789/1‑150 (MQ=255)
                                                                                                  tGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCa                                               >  1:460372/1‑150 (MQ=255)
                                                                                                   gCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCAc                                              <  1:13679/150‑1 (MQ=255)
                                                                                                    cACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACg                                             >  2:1652352/1‑150 (MQ=255)
                                                                                                    cACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACg                                             >  2:2345177/1‑150 (MQ=255)
                                                                                                     aCCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGt                                            >  1:974057/1‑150 (MQ=255)
                                                                                                             cGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATa                                    >  2:2462195/1‑150 (MQ=255)
                                                                                                              gTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATAc                                   >  1:943667/1‑150 (MQ=255)
                                                                                                              gTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATAc                                   >  2:2827748/1‑150 (MQ=255)
                                                                                                               tAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACa                                  >  1:2793724/1‑150 (MQ=255)
                                                                                                                aaaaTTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAg                                 <  1:2361677/150‑1 (MQ=255)
                                                                                                                aaaaTTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAg                                 <  1:2317017/150‑1 (MQ=255)
                                                                                                                aaaaTTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAg                                 <  1:1865552/150‑1 (MQ=255)
                                                                                                                   aTTTTGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGt                              >  1:2833128/1‑150 (MQ=255)
                                                                                                                       tGTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTg                          <  1:1571754/150‑1 (MQ=255)
                                                                                                                        gTTTCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGc                         <  2:1155234/150‑1 (MQ=255)
                                                                                                                         tttCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCt                        <  2:147342/150‑1 (MQ=255)
                                                                                                                          ttCGAGTTAGGCAACATCTGCATATAACGAAGCAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtt                       >  2:2264562/1‑150 (MQ=255)
                                                                                                                           tCGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCttt                      <  1:1648415/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     <  1:1022736/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     <  2:151302/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     <  1:2551015/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     <  2:1676319/150‑1 (MQ=255)
                                                                                                                            cGAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCtttt                     >  2:297001/1‑150 (MQ=255)
                                                                                                                             gAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTc                    <  2:3612824/150‑1 (MQ=255)
                                                                                                                             gAGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTc                    >  2:1814937/1‑150 (MQ=255)
                                                                                                                              aGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTcc                   >  1:1543731/1‑150 (MQ=255)
                                                                                                                              aGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTcc                   >  1:2869942/1‑150 (MQ=255)
                                                                                                                              aGTTAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATAGCTTTTcc                   >  1:746059/1‑150 (MQ=255)
                                                                                                                                 tAGGCAACATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCcaca                >  1:2929329/1‑150 (MQ=255)
                                                                                                                                      aaCATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCt           >  1:2041151/1‑150 (MQ=255)
                                                                                                                                        cATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTgg         <  2:3083759/150‑1 (MQ=255)
                                                                                                                                        cATCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTgg         >  2:1623582/1‑150 (MQ=255)
                                                                                                                                         aTCTGCATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGa        >  2:363599/1‑150 (MQ=255)
                                                                                                                                              cATATAACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGtt   <  2:210548/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AATCTGTAATCGCGAACGGGCCAGGAGGAACCTCTTTCTGATAAACCACAAAACCATTCTGTTCAATAGTTACCAGCGCGTTACTCTGAGCAATCCCCTGCACCCGTGGCGTAAAATTTTGTTTCGAGTTAGGCAACATCTGCATATCACGAAACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTGGATTGTTA  >  CP009273/2183495‑2183787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑