BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,241,793	A→T	A73A (GCT→GCA)	lysP ←	lysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,241,793	0	A	T	99.6%	791.5 / NA	242	A73A (GCT→GCA)	lysP	lysine transporter
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (131/110); total (132/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

CTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAAT  >  CP009273/2241647‑2241939

cTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGcc                                                                                                                                                 <  2:2913273/150‑1 (MQ=255)

cTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGcc                                                                                                                                                 >  2:3570294/1‑150 (MQ=255)

 tCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCa                                                                                                                                                >  2:1497219/1‑150 (MQ=255)

 tCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCa                                                                                                                                                <  1:1415607/150‑1 (MQ=255)

   aTGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGt                                                                                                                                              >  1:1291417/1‑150 (MQ=255)

    tGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGtt                                                                                                                                             <  1:2119868/150‑1 (MQ=255)

    tGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGtt                                                                                                                                             <  1:232613/150‑1 (MQ=255)

      aCCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCa                                                                                                                                           >  2:616553/1‑150 (MQ=255)

        cAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCAcc                                                                                                                                         <  2:1196041/150‑1 (MQ=255)

          gctgagctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCga                                                                                                                                       <  1:487742/150‑1 (MQ=255)

           ctgagctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCgag                                                                                                                                      <  1:431862/150‑1 (MQ=255)

                ctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTgg                                                                                                                                 >  1:3080027/1‑150 (MQ=255)

                ctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTgg                                                                                                                                 <  2:567776/150‑1 (MQ=255)

                 tgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGt                                                                                                                                <  1:1069439/150‑1 (MQ=255)

                   cAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGtca                                                                                                                              <  1:1370979/150‑1 (MQ=255)

                   cAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGtca                                                                                                                              >  1:2354100/1‑150 (MQ=255)

                   cAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGtca                                                                                                                              <  1:989461/150‑1 (MQ=255)

                    aaCCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGtcat                                                                                                                             >  1:2374967/1‑150 (MQ=255)

                    aaCCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGtcat                                                                                                                             <  1:1686201/150‑1 (MQ=255)

                     aCCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGtcatc                                                                                                                            <  2:2102768/150‑1 (MQ=255)

                       cAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAg                                                                                                                          >  2:1042566/1‑150 (MQ=255)

                       cAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAg                                                                                                                          <  1:2682347/150‑1 (MQ=255)

                         ggTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGa                                                                                                                        >  1:2304948/1‑150 (MQ=255)

                         ggTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGa                                                                                                                        >  2:3072660/1‑150 (MQ=255)

                          gTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGaa                                                                                                                       <  2:2119238/150‑1 (MQ=255)

                           tCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAg                                                                                                                      >  2:3309972/1‑150 (MQ=255)

                            cAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGt                                                                                                                     <  2:1295851/150‑1 (MQ=255)

                             aaCGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTa                                                                                                                    >  2:1439738/1‑150 (MQ=255)

                                ggCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAAc                                                                                                                 >  2:667440/1‑150 (MQ=255)

                                ggCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAAc                                                                                                                 >  2:1617651/1‑150 (MQ=255)

                                  cGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCa                                                                                                               >  2:1851776/1‑150 (MQ=255)

                                         cACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGcc                                                                                                        <  2:3408633/150‑1 (MQ=255)

                                           ccGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCaa                                                                                                      <  1:1350469/150‑1 (MQ=255)

                                            cGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAAt                                                                                                     >  2:1814662/1‑150 (MQ=255)

                                              cccAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCa                                                                                                   <  2:2643626/150‑1 (MQ=255)

                                              cccAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCa                                                                                                   <  2:1809363/150‑1 (MQ=255)

                                               ccAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGATACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAg                                                                                                  <  1:3576256/150‑1 (MQ=255)

                                               ccAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAg                                                                                                  <  1:2031181/150‑1 (MQ=255)

                                                 aGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCa                                                                                                >  1:10321/1‑150 (MQ=255)

                                                  gTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCat                                                                                               <  1:1443826/150‑1 (MQ=255)

                                                      tACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAc                                                                                           <  1:3100341/150‑1 (MQ=255)

                                                         cAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACgag                                                                                        <  1:599288/150‑1 (MQ=255)

                                                         cAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACgag                                                                                        >  1:1048292/1‑150 (MQ=255)

                                                         cAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTACCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACgag                                                                                        >  2:3644881/1‑150 (MQ=255)

                                                           gtagtTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGagag                                                                                      <  1:1745642/150‑1 (MQ=255)

                                                             agtTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCa                                                                                    <  2:2076463/150‑1 (MQ=255)

                                                                    gcccagcGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGcccc                                                                             <  2:2533960/150‑1 (MQ=255)

                                                                     cccagcGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATTCGAGAGCAATGCcccg                                                                            <  1:1198501/150‑1 (MQ=255)

                                                                      ccagcGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgc                                                                           >  2:1516104/1‑150 (MQ=255)

                                                                       cagcGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcc                                                                          >  1:3400384/1‑150 (MQ=255)

                                                                        agcGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgccc                                                                         <  1:2295159/150‑1 (MQ=255)

                                                                          cgcgAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCgg                                                                       <  1:3382209/150‑1 (MQ=255)

                                                                           gcgAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGa                                                                      >  2:1279715/1‑150 (MQ=255)

                                                                            cgAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAc                                                                     >  1:1617797/1‑150 (MQ=255)

                                                                               aGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctg                                                                  <  2:2327371/150‑1 (MQ=255)

                                                                                gCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgc                                                                 >  1:2500111/1‑150 (MQ=255)

                                                                                  cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                                                               <  1:3240426/150‑1 (MQ=255)

                                                                                  cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                                                               >  1:2292765/1‑150 (MQ=255)

                                                                                   aaaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATCTATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcctg                                                              <  2:3457807/150‑1 (MQ=255)

                                                                                   aaaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcctg                                                              <  1:2198361/150‑1 (MQ=255)

                                                                                   aaaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcctg                                                              <  1:289618/150‑1 (MQ=255)

                                                                                    aaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTga                                                             <  1:3523552/150‑1 (MQ=255)

                                                                                    aaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTga                                                             <  2:1243548/150‑1 (MQ=255)

                                                                                     aGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTgag                                                            >  2:2957482/1‑150 (MQ=255)

                                                                                      gCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTgaga                                                           >  2:368442/1‑150 (MQ=255)

                                                                                      gCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTgaga                                                           <  2:1895875/150‑1 (MQ=255)

                                                                                        cttcttCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGaaa                                                         >  1:1892219/1‑150 (MQ=255)

                                                                                          tcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATc                                                       >  1:295326/1‑150 (MQ=255)

                                                                                           cttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCg                                                      <  2:3080027/150‑1 (MQ=255)

                                                                                           cttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCg                                                      >  1:222533/1‑150 (MQ=255)

                                                                                            ttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCga                                                     <  1:375890/150‑2 (MQ=255)

                                                                                            ttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGt                                                     >  1:2729575/1‑150 (MQ=255)

                                                                                             tcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGtt                                                    <  2:430053/150‑1 (MQ=255)

                                                                                               aaCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgc                                                  >  2:3158874/1‑150 (MQ=255)

                                                                                                      ttCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCagag                                           >  2:834001/1‑150 (MQ=255)

                                                                                                       tCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAGATCGTTGCGCCAGAgg                                          >  1:609766/1‑150 (MQ=255)

                                                                                                       tCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAgg                                          <  2:1712194/150‑1 (MQ=255)

                                                                                                         tGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGca                                        >  2:3773768/1‑150 (MQ=255)

                                                                                                         tGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGca                                        >  1:2623031/1‑150 (MQ=255)

                                                                                                            ccGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGcaaca                                     >  2:2172788/1‑150 (MQ=255)

                                                                                                             cGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGcaacaa                                    >  2:1220877/1‑150 (MQ=255)

                                                                                                              gTAAGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACaaa                                   >  2:2847897/1‑150 (MQ=255)

                                                                                                                 aGTGGCAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAg                                >  2:193367/1‑150 (MQ=255)

                                                                                                                      cAAACGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTg                           >  1:354947/1‑150 (MQ=255)

                                                                                                                       aaaCGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCATCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGt                          <  1:1764083/150‑1 (MQ=255)

                                                                                                                        aaCGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTa                         <  1:1617651/150‑1 (MQ=255)

                                                                                                                          cGAACCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTAcc                       >  1:3689701/1‑150 (MQ=255)

                                                                                                                             aCCGGAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGAt                    <  1:2814492/150‑1 (MQ=255)

                                                                                                                                ggAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGa                 >  2:2364691/1‑150 (MQ=255)

                                                                                                                                 gAAACCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGaa                >  2:3048184/1‑150 (MQ=255)

                                                                                                                                   aaCCGGCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAAcc              <  1:972873/150‑1 (MQ=255)

                                                                                                                                       ggCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCa          >  1:3580554/1‑150 (MQ=255)

                                                                                                                                        gCATATATGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCaa         >  1:1186154/1‑150 (MQ=255)

                                                                                                                                          atatatGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATg       >  1:2449330/1‑150 (MQ=255)

                                                                                                                                           tatatGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATgg      >  1:3791215/1‑150 (MQ=255)

                                                                                                                                            atatGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGc     <  2:923116/150‑1 (MQ=255)

                                                                                                                                            atatGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGc     >  1:1854657/1‑150 (MQ=255)

                                                                                                                                            atatGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGc     >  2:222009/1‑150 (MQ=255)

                                                                                                                                             tatGCTGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCa    >  1:716907/1‑150 (MQ=255)

                                                                                                                                               tgctgcCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAat  >  2:2508517/1‑150 (MQ=255)

CTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAAT  >  CP009273/2241647‑2241939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`