Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,411,701 T→A L381* (TTA→TAA)  yfcC → putative inner membrane transporter, C4‑dicarboxylate anaerobic carrier family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,411,7010TA100.0% 744.7 / NA 223L381* (TTA→TAA) yfcCputative inner membrane transporter, C4‑dicarboxylate anaerobic carrier family
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (103/120);  total (103/120)

Only 100 of 224 total aligned reads displayed.

TCCGCCTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGG  >  CP009273/2411554‑2411847
                                                                                                                                                   |                                                                                                                                                  
tCCGCCTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTaaa                                                                                                                                                  >  2:1422517/1‑150 (MQ=255)
  cGCCTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAAt                                                                                                                                                <  1:1278656/150‑1 (MQ=255)
  cGCCTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAAt                                                                                                                                                <  1:2248578/150‑1 (MQ=255)
   gCCTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATa                                                                                                                                               >  1:2653424/1‑150 (MQ=255)
    ccTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATAc                                                                                                                                              >  2:3153672/1‑150 (MQ=255)
    ccTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATAc                                                                                                                                              <  2:1332147/150‑1 (MQ=255)
    ccTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATAc                                                                                                                                              <  1:1656939/150‑1 (MQ=255)
      ttAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCa                                                                                                                                            <  1:1407073/150‑1 (MQ=255)
      ttAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCa                                                                                                                                            >  2:2988310/1‑150 (MQ=255)
           ggCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCCGCGTGTAAAATACCATCCTc                                                                                                                                       >  2:1313354/1‑150 (MQ=255)
           ggCATGACGGTAAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGAAGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTc                                                                                                                                       >  2:1862100/1‑150 (MQ=255)
            gCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCa                                                                                                                                      <  2:1619489/150‑1 (MQ=255)
                 aCGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGc                                                                                                                                 <  1:774272/150‑1 (MQ=255)
                  cGGTTAATACCATGGCTTCATCCTTTACCGAGGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCa                                                                                                                                >  1:891838/1‑150 (MQ=255)
                  cGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCa                                                                                                                                <  1:1512877/150‑1 (MQ=255)
                   ggTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCAt                                                                                                                               <  2:2734551/150‑1 (MQ=255)
                   ggTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCAt                                                                                                                               <  2:3440650/150‑1 (MQ=255)
                   ggTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCAt                                                                                                                               <  1:1559732/150‑1 (MQ=255)
                          aCCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAAt                                                                                                                        <  1:710903/150‑1 (MQ=255)
                             aTGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGcc                                                                                                                     >  1:2928680/1‑150 (MQ=255)
                             aTGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGcc                                                                                                                     >  2:2812603/1‑150 (MQ=255)
                                gCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCAtt                                                                                                                  <  1:2532375/150‑1 (MQ=255)
                                 cTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTa                                                                                                                 <  2:1921823/150‑1 (MQ=255)
                                 cTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTa                                                                                                                 <  1:2519582/150‑1 (MQ=255)
                                 cTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTa                                                                                                                 <  1:3288545/150‑1 (MQ=255)
                                  ttCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAg                                                                                                                <  1:2158617/150‑1 (MQ=255)
                                  ttCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAg                                                                                                                <  1:2829895/150‑1 (MQ=255)
                                       ccTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTc                                                                                                           >  1:1064560/1‑150 (MQ=255)
                                        cTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCt                                                                                                          >  1:1694650/1‑150 (MQ=255)
                                        cTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCt                                                                                                          >  1:3684109/1‑150 (MQ=255)
                                        cTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCt                                                                                                          >  1:3610936/1‑150 (MQ=255)
                                           tACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGa                                                                                                       >  1:2834389/1‑150 (MQ=255)
                                               gAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAAc                                                                                                   >  2:3495955/1‑150 (MQ=255)
                                               gAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAAc                                                                                                   >  2:1069975/1‑150 (MQ=255)
                                                aaGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAAcg                                                                                                  <  2:3510302/150‑1 (MQ=255)
                                                 aGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAAcgc                                                                                                 >  1:3738688/1‑150 (MQ=255)
                                                    ggCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGt                                                                                              <  1:319399/150‑1 (MQ=255)
                                                     gcgcGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTc                                                                                             <  2:1122420/150‑1 (MQ=255)
                                                     gcgcGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTc                                                                                             <  2:757942/150‑1 (MQ=255)
                                                        cgAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTcgcg                                                                                          >  2:3754451/1‑150 (MQ=255)
                                                         gAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCgg                                                                                         <  1:2120678/150‑1 (MQ=255)
                                                          aaTGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGc                                                                                        >  2:3013177/1‑150 (MQ=255)
                                                          aaTGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGc                                                                                        >  2:2836426/1‑150 (MQ=255)
                                                            tgatgaTCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCt                                                                                      <  1:452666/150‑1 (MQ=255)
                                                            tgatgaTCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCt                                                                                      <  1:1970954/150‑1 (MQ=255)
                                                             gatgatCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTg                                                                                     >  2:2966770/1‑150 (MQ=255)
                                                                    gCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATg                                                                              >  2:2396220/1‑150 (MQ=255)
                                                                    gCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATg                                                                              >  1:3558646/1‑150 (MQ=255)
                                                                      ccctgccctgCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGtt                                                                            <  2:456295/150‑1 (MQ=255)
                                                                       cctgccctgcTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTg                                                                           <  1:20846/150‑1 (MQ=255)
                                                                            cctgcTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCtt                                                                      <  2:503384/150‑1 (MQ=255)
                                                                              tgctgGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTcc                                                                    >  1:2735316/1‑150 (MQ=255)
                                                                              tgctgGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTcc                                                                    >  1:3629867/1‑150 (MQ=255)
                                                                               gctgGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCa                                                                   <  2:2320943/150‑1 (MQ=255)
                                                                                ctgGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAg                                                                  >  1:1014978/1‑150 (MQ=255)
                                                                                 tggtggGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAgg                                                                 >  1:1482959/1‑150 (MQ=255)
                                                                                 tggtggGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAgg                                                                 <  1:1883801/150‑1 (MQ=255)
                                                                                   gtggGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCg                                                               >  1:2850120/1‑150 (MQ=255)
                                                                                    tggGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCgg                                                              >  1:3317974/1‑150 (MQ=255)
                                                                                    tggGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCgg                                                              >  2:3803806/1‑150 (MQ=255)
                                                                                      ggTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTa                                                            <  2:526633/150‑1 (MQ=255)
                                                                                         ttCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTAttt                                                         <  1:1289462/150‑1 (MQ=255)
                                                                                         ttCGCCAAAGGGATTATGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTAttt                                                         >  2:1789246/1‑150 (MQ=255)
                                                                                            gCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAAt                                                      <  2:2736581/150‑1 (MQ=255)
                                                                                            gCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAAt                                                      <  1:2988310/150‑1 (MQ=255)
                                                                                            gCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAAt                                                      >  2:173830/1‑150 (MQ=255)
                                                                                             ccAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAAtt                                                     <  1:3448338/150‑1 (MQ=255)
                                                                                             ccAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAAtt                                                     <  1:3549191/150‑1 (MQ=255)
                                                                                              cAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAAttt                                                    <  1:2402266/150‑1 (MQ=255)
                                                                                               aaaGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATttc                                                   <  1:3013177/150‑1 (MQ=255)
                                                                                                aaGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATttct                                                  >  1:1447263/1‑150 (MQ=255)
                                                                                                aaGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATttct                                                  <  2:1219331/150‑1 (MQ=255)
                                                                                                 aGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTa                                                                                >  1:2488839/1‑119 (MQ=255)
                                                                                                 aGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTa                                                                                <  2:2488839/119‑1 (MQ=255)
                                                                                                  gggATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATttcttc                                                <  2:785758/150‑1 (MQ=255)
                                                                                                  gggATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATttcttc                                                <  1:2607940/150‑1 (MQ=255)
                                                                                                   ggATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCg                                               >  1:1048001/1‑150 (MQ=255)
                                                                                                      ttttGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGa                                            >  2:64382/1‑150 (MQ=255)
                                                                                                           ctgctgGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTcc                                       >  2:632617/1‑150 (MQ=255)
                                                                                                                 gTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCt                                 >  2:2167479/1‑150 (MQ=255)
                                                                                                                 gTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCt                                 >  2:1764448/1‑150 (MQ=255)
                                                                                                                 gTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCt                                 <  2:3071269/150‑1 (MQ=255)
                                                                                                                 gTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCt                                 >  2:3658388/1‑150 (MQ=255)
                                                                                                                  tCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTg                                >  1:3779530/1‑150 (MQ=255)
                                                                                                                   cGGTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTgg                               <  2:1060280/150‑1 (MQ=255)
                                                                                                                    ggTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGt                              >  1:1880810/1‑150 (MQ=255)
                                                                                                                    ggTAATGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGt                              >  2:2824778/1‑150 (MQ=255)
                                                                                                                       aaTGGTGAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAg                           >  1:1941657/1‑150 (MQ=255)
                                                                                                                             gAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGgcggcg                     >  1:1233492/1‑150 (MQ=255)
                                                                                                                             gAAGCGGGTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGgcggcg                     <  2:2785310/150‑1 (MQ=255)
                                                                                                                                   ggTGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAAcc               <  2:3610936/150‑1 (MQ=255)
                                                                                                                                     tGATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCAt             <  2:1160830/150‑1 (MQ=255)
                                                                                                                                      gATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCTGCGTTAACCATg            <  1:1177395/150‑1 (MQ=255)
                                                                                                                                      gATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATg            <  1:2812603/150‑1 (MQ=255)
                                                                                                                                      gATGCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGCTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATg            >  2:2598051/1‑150 (MQ=255)
                                                                                                                                         gCCAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCg         <  2:2888681/150‑1 (MQ=255)
                                                                                                                                           cAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGtt       >  1:3089608/1‑150 (MQ=255)
                                                                                                                                           cAGCGTGTAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGtt       <  2:3317936/150‑1 (MQ=255)
                                                                                                                                                tgtAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTgg  <  1:1247979/150‑1 (MQ=255)
                                                                                                                                                tgtAAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTgg  <  2:2136855/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCGCCTTAACGGCATGACGGTTAATACCATGGCTTCATCCTTTACCGAAGGGGCGCGAATGATGATCGCCCCTGCCCTGCTGGTGGGTTTCGCCAAAGGGATTTTGCTGCTGGTCGGTAATGGTGAAGCGGGTGATGCCAGCGTGTTAAATACCATCCTCAACAGCATTGCCAATGCCATTAGCGGTCTGGACAACGCGGTCGCGGCCTGGTTTATGTTGCTCTTCCAGGCGGTATTTAATTTCTTCGTGACGTCCGGTTCTGGTCAGGCGGCGTTAACCATGCCGTTACTGG  >  CP009273/2411554‑2411847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑