Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,481,488 A→T intergenic (+42/+14) evgS → / ← yfdE hybrid sensory histidine kinase in two‑component regulatory system with EvgA/acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,481,4880AT100.0% 904.4 / NA 269intergenic (+42/+14)evgS/yfdEhybrid sensory histidine kinase in two‑component regulatory system with EvgA/acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (150/119);  total (150/119)

Only 100 of 269 total aligned reads displayed.

TTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAAT  >  CP009273/2481341‑2481633
                                                                                                                                                   |                                                                                                                                                 
  cAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGcac                                                                                                                                               >  1:1433023/1‑150 (MQ=255)
    gATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACAt                                                                                                                                             >  1:2274847/1‑150 (MQ=255)
      tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCt                                                                                                                                           >  2:2100696/1‑150 (MQ=255)
      tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCt                                                                                                                                           >  1:588731/1‑150 (MQ=255)
      tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCt                                                                                                                                           <  2:1880625/150‑1 (MQ=255)
      tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCt                                                                                                                                           >  1:1782363/1‑150 (MQ=255)
      tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTAAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTTCACATCt                                                                                                                                           >  1:2493165/1‑150 (MQ=255)
       gACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCtt                                                                                                                                          <  2:3658036/150‑1 (MQ=255)
        aCAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTa                                                                                                                                         >  2:3260098/1‑150 (MQ=255)
            tAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACAtt                                                                                                                                     >  1:1165986/1‑150 (MQ=255)
             aaGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACAttt                                                                                                                                    <  1:2965143/150‑1 (MQ=255)
              aGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTa                                                                                                                                   >  2:1433052/1‑150 (MQ=255)
              aGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTa                                                                                                                                   >  1:2818173/1‑150 (MQ=255)
                   gAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTatgatg                                                                                                                              <  2:2170000/150‑1 (MQ=255)
                     aaTTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATgag                                                                                                                            <  2:3365314/150‑1 (MQ=255)
                           tcAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTcc                                                                                                                      >  2:3039576/1‑150 (MQ=255)
                            cAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCt                                                                                                                     >  2:2768415/1‑150 (MQ=255)
                              gTTGATGAACTCTGTATATGAACACATTGAAGAGATGGACCAGGAGATTGCTGTTTTCTGTAAGAAAAATGACTAAATAGCGGCTCACACAATGTTCAAATGTGTGAGCTATTTACCTGCACATATTACATTTATGATGAGAACTCCTgg                                                                                                                   <  1:3463737/150‑1 (MQ=255)
                                tGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCg                                                                                                                 >  2:1392323/1‑150 (MQ=255)
                                 gCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGa                                                                                                                >  1:1301222/1‑150 (MQ=255)
                                  cTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGaa                                                                                                               >  2:3290244/1‑150 (MQ=255)
                                    gAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAATTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTCTTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAAtt                                                                                                             >  2:2916273/1‑150 (MQ=255)
                                     aaCTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGTGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAAttt                                                                                                            >  2:3307698/1‑150 (MQ=255)
                                          tGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTcc                                                                                                       >  1:3588969/1‑150 (MQ=255)
                                           gTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTccc                                                                                                      >  2:456855/1‑150 (MQ=255)
                                             aaaaGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCa                                                                                                    >  2:3682457/1‑150 (MQ=255)
                                              aaaGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCAt                                                                                                   <  1:1556477/150‑1 (MQ=255)
                                               aaGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATg                                                                                                  <  1:2768415/150‑1 (MQ=255)
                                                 gAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCt                                                                                                <  2:1146736/150‑1 (MQ=255)
                                                  aaCACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTg                                                                                               <  2:92947/150‑1 (MQ=255)
                                                  aaCACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTg                                                                                               >  2:1834369/1‑150 (MQ=255)
                                                    cacaTTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGt                                                                                             <  1:2726046/150‑1 (MQ=255)
                                                       aTTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGa                                                                                          >  1:1245369/1‑150 (MQ=255)
                                                        ttGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAg                                                                                         <  2:3312442/150‑1 (MQ=255)
                                                          gCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCg                                                                                       <  2:575083/150‑1 (MQ=255)
                                                           cAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGt                                                                                      <  1:1457059/150‑1 (MQ=255)
                                                               gCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGcc                                                                                  <  1:3665188/150‑1 (MQ=255)
                                                                    aCCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTcc                                                                             >  2:727895/1‑150 (MQ=255)
                                                                     ccAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTACAGAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTccc                                                                            <  1:2333380/150‑1 (MQ=255)
                                                                      cAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCg                                                                           >  1:3135609/1‑150 (MQ=255)
                                                                      cAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCg                                                                           >  2:2081615/1‑150 (MQ=255)
                                                                       aGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCgg                                                                          >  1:1107023/1‑150 (MQ=255)
                                                                       aGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCgg                                                                          >  1:213923/1‑150 (MQ=255)
                                                                          agaTTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCAt                                                                       >  2:2505827/1‑150 (MQ=255)
                                                                              tGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACa                                                                   <  1:3328386/150‑1 (MQ=255)
                                                                               gCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACAt                                                                  >  1:564533/1‑150 (MQ=255)
                                                                                 tGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGc                                                                >  2:2465725/1‑150 (MQ=255)
                                                                                 tGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGc                                                                <  1:2400796/150‑1 (MQ=255)
                                                                                 tGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGc                                                                <  1:2100710/150‑1 (MQ=255)
                                                                                  gTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCg                                                               >  1:2629424/1‑150 (MQ=255)
                                                                                   ttttCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCgg                                                              >  1:994201/1‑150 (MQ=255)
                                                                                    tttCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCggg                                                             <  1:1831747/150‑1 (MQ=255)
                                                                                      tCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTc                                                           <  2:1162133/150‑1 (MQ=255)
                                                                                      tCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTc                                                           <  2:1163367/150‑1 (MQ=255)
                                                                                       cTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTcc                                                          >  1:318022/1‑150 (MQ=255)
                                                                                       cTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTcc                                                          <  2:1355011/150‑1 (MQ=255)
                                                                                          tCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCcgcg                                                       <  2:1301261/150‑1 (MQ=255)
                                                                                           cAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCgcgc                                                      >  1:2521562/1‑150 (MQ=255)
                                                                                           cAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCgcgc                                                      >  1:1011397/1‑150 (MQ=255)
                                                                                             gAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAg                                                    <  1:16460/150‑1 (MQ=255)
                                                                                               aaaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGcc                                                  >  1:2219311/1‑150 (MQ=255)
                                                                                                aaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCg                                                 >  1:782475/1‑150 (MQ=255)
                                                                                                aaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCg                                                 >  1:3275236/1‑150 (MQ=255)
                                                                                                  aTGACTAAATAGCGGCTCCCACAATGTTCAGATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCt                                               >  2:3133742/1‑150 (MQ=255)
                                                                                                    gACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGa                                             >  1:1164301/1‑150 (MQ=255)
                                                                                                       tAAAGAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAttt                                          <  1:3705915/150‑1 (MQ=255)
                                                                                                         aaTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAttttt                                        >  2:3000341/1‑150 (MQ=255)
                                                                                                         aaTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAttttt                                        <  2:2818173/150‑1 (MQ=255)
                                                                                                          aTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTa                                       >  1:1325857/1‑150 (MQ=255)
                                                                                                           tAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTAt                                      <  1:1880699/150‑1 (MQ=255)
                                                                                                           tAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTAt                                      >  2:1652218/1‑150 (MQ=255)
                                                                                                           gaGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTAt                                      <  2:2739048/149‑1 (MQ=255)
                                                                                                            aGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATc                                     <  2:1559573/150‑1 (MQ=255)
                                                                                                            aGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATc                                     <  1:3008090/150‑1 (MQ=255)
                                                                                                               ggCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGa                                  >  1:2544750/1‑150 (MQ=255)
                                                                                                               ggCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCCCTGATTTTTATCGGa                                  <  2:2148186/150‑1 (MQ=255)
                                                                                                                gCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAt                                 <  2:1245369/150‑1 (MQ=255)
                                                                                                                gCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAt                                 >  1:2150084/1‑150 (MQ=255)
                                                                                                                 cTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAtt                                >  1:2025920/1‑150 (MQ=255)
                                                                                                                  tCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTa                               >  1:1974940/1‑150 (MQ=255)
                                                                                                                  tCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTa                               <  2:1821600/150‑1 (MQ=255)
                                                                                                                   cccACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTAc                              <  1:3290244/150‑1 (MQ=255)
                                                                                                                   cccACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTAc                              <  2:3198904/150‑1 (MQ=255)
                                                                                                                     cacaATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTAccc                            <  2:3574097/150‑1 (MQ=255)
                                                                                                                         aTGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGca                        <  1:3501646/150‑1 (MQ=255)
                                                                                                                          tGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGATGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcat                       >  2:1325300/1‑150 (MQ=255)
                                                                                                                            ttCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcatca                     >  2:370153/1‑150 (MQ=255)
                                                                                                                              cAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatt                   >  2:1052946/1‑150 (MQ=255)
                                                                                                                              cAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatt                   >  2:1214559/1‑150 (MQ=255)
                                                                                                                              cAAATGTGGGAGCTATTTACCTGCACATCTTACAGTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatt                   >  2:2220317/1‑150 (MQ=255)
                                                                                                                               aaaTGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatta                  <  1:456748/150‑1 (MQ=255)
                                                                                                                                 aTGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCattatt                >  2:1631289/1‑150 (MQ=255)
                                                                                                                                 aTGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCattatt                <  2:249763/150‑1 (MQ=255)
                                                                                                                                 aTGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCattatt                >  2:1631204/1‑150 (MQ=255)
                                                                                                                                    tgGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTccc             <  1:1392323/150‑1 (MQ=255)
                                                                                                                                       gAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGTGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCg          <  1:737660/150‑1 (MQ=255)
                                                                                                                                        aGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCAGCTGATTTTTATCGGATTACCCGGCAACATTATTCCCCCgg         >  1:3043947/1‑150 (MQ=255)
                                                                                                                                            aTTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTtc     <  2:1958234/150‑1 (MQ=255)
                                                                                                                                              ttACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTAATCCCCCTGCTtcaa   >  1:2902327/1‑150 (MQ=255)
                                                                                                                                               tACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTtcaat  <  1:1077762/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAAT  >  CP009273/2481341‑2481633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑