BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,512,031	C→A	S29* (TCA→TAA)	yfeC →	DUF1323 family putative DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,512,031	0	C	A	100.0%	669.7 / NA	200	S29* (TCA→TAA)	yfeC	DUF1323 family putative DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (114/86); total (114/86)

Only 100 of 200 total aligned reads displayed.

TTATAAAATGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTG  >  CP009273/2511884‑2512176

         gAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTg                                                                                                                                        <  1:3313200/150‑1 (MQ=255)

         gAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTg                                                                                                                                        <  2:1173337/150‑1 (MQ=255)

          aaCTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTgg                                                                                                                                       >  1:246047/1‑150 (MQ=255)

             tactaATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGt                                                                                                                                    >  2:2643776/1‑150 (MQ=255)

                 aaTAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAg                                                                                                                                >  1:3722664/1‑150 (MQ=255)

                  aTAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAgg                                                                                                                               >  2:1232785/1‑150 (MQ=255)

                  aTAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAgg                                                                                                                               <  2:3635102/150‑1 (MQ=255)

                  aTAGACCCACATACAGTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAgg                                                                                                                               <  2:3661108/150‑1 (MQ=255)

                    aGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGt                                                                                                                             >  2:84848/1‑150 (MQ=255)

                    aGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGt                                                                                                                             <  2:2721111/150‑1 (MQ=255)

                     gACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTg                                                                                                                            <  2:3042896/150‑1 (MQ=255)

                         cacaTACATTCAGGGAATTGTTATGTACAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCaa                                                                                                                        >  2:1339785/1‑150 (MQ=255)

                          acatacatTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCaaa                                                                                                                       >  1:1113943/1‑150 (MQ=255)

                             tacatTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGcc                                                                                                                    <  1:3248702/150‑1 (MQ=255)

                              acatTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGccc                                                                                                                   <  1:3635217/150‑1 (MQ=255)

                               catTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCg                                                                                                                  >  1:391882/1‑150 (MQ=255)

                                  tCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACt                                                                                                               <  1:3482017/150‑1 (MQ=255)

                                  tCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACt                                                                                                               >  2:1043570/1‑150 (MQ=255)

                                   cAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTg                                                                                                              <  1:1912558/150‑1 (MQ=255)

                                    aGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTgg                                                                                                             >  1:1083364/1‑150 (MQ=255)

                                       gAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTc                                                                                                          >  2:3273756/1‑150 (MQ=255)

                                       gAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTc                                                                                                          >  1:2095431/1‑150 (MQ=255)

                                       gAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTc                                                                                                          >  2:3095244/1‑150 (MQ=255)

                                        aaTTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACACCGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCa                                                                                                         >  1:3782841/1‑150 (MQ=255)

                                        aaTTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCa                                                                                                         <  1:2312797/150‑1 (MQ=255)

                                        aaTTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCa                                                                                                         >  1:1720944/1‑150 (MQ=255)

                                          ttGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACg                                                                                                       <  2:1135675/150‑1 (MQ=255)

                                           tGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGt                                                                                                      <  1:1809044/150‑1 (MQ=255)

                                           tGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGt                                                                                                      <  1:3703520/150‑1 (MQ=255)

                                           tGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGt                                                                                                      >  2:1626262/1‑150 (MQ=255)

                                               aTGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAAt                                                                                                  <  2:69040/150‑1 (MQ=255)

                                               aTGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAAt                                                                                                  <  2:1067656/150‑1 (MQ=255)

                                                  ttCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGaa                                                                                               <  2:1094898/150‑1 (MQ=255)

                                                   tCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAAc                                                                                              <  2:36886/150‑1 (MQ=255)

                                                     aaGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAg                                                                                            >  1:2852325/1‑150 (MQ=255)

                                                      aGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAgg                                                                                           >  2:2060997/1‑150 (MQ=255)

                                                      aGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAgg                                                                                           >  2:441927/1‑150 (MQ=255)

                                                       ggAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGt                                                                                          >  2:337285/1‑150 (MQ=255)

                                                        gagaGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCACAGCCCGACTGGTTCACGTCAATGAACAGGtt                                                                                         >  1:708246/1‑150 (MQ=255)

                                                        gagaGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGtt                                                                                         >  1:1303346/1‑150 (MQ=255)

                                                           agGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGt                                                                                      >  2:748071/1‑150 (MQ=255)

                                                             gATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGa                                                                                    >  1:1223298/1‑150 (MQ=255)

                                                              aTGACACCAGATGAACTTGCCAGACTGACCGGCGATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGaa                                                                                   <  2:3383361/150‑1 (MQ=255)

                                                                 acacCAGATGAACGTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAatat                                                                                <  2:2283647/150‑1 (MQ=255)

                                                                   acCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAatatat                                                                              >  1:1515751/1‑150 (MQ=255)

                                                                   acCAGATGAACTTGCCAGACTGACCGGATATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAatatat                                                                              <  2:1031297/150‑1 (MQ=255)

                                                                    ccAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATAtt                                                                             >  2:246230/1‑150 (MQ=255)

                                                                    ccAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATAtt                                                                             <  2:77254/150‑1 (MQ=255)

                                                                    ccAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTCCACGTCAATGAACAGGTTCGTGAATATAtt                                                                             <  2:1597059/150‑1 (MQ=255)

                                                                     cAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTc                                                                            <  1:53523/150‑1 (MQ=255)

                                                                        aTGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCa                                                                         >  2:1939320/1‑150 (MQ=255)

                                                                           aaCTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATg                                                                      >  1:686510/1‑150 (MQ=255)

                                                                           aaCTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATg                                                                      >  1:1486318/1‑150 (MQ=255)

                                                                           aaCTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATg                                                                      >  2:455978/1‑150 (MQ=255)

                                                                              ttGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTg                                                                   >  1:1788697/1‑150 (MQ=255)

                                                                              ttGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTg                                                                   <  1:3593660/150‑1 (MQ=255)

                                                                               tGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGa                                                                  <  2:2063591/150‑1 (MQ=255)

                                                                               tGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGa                                                                  >  1:1931619/1‑150 (MQ=255)

                                                                                gCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGaa                                                                 <  2:2493264/150‑1 (MQ=255)

                                                                                   aGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGt                                                              >  2:2443218/1‑150 (MQ=255)

                                                                                     aCTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTcc                                                            <  1:1181063/150‑1 (MQ=255)

                                                                                     aCTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTcc                                                            <  2:1724306/150‑1 (MQ=255)

                                                                                      cTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCa                                                           >  1:1445099/1‑150 (MQ=255)

                                                                                       tGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAg                                                          >  2:1029833/1‑150 (MQ=255)

                                                                                       tGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAg                                                          <  1:3019930/150‑1 (MQ=255)

                                                                                       tGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAg                                                          >  1:979680/1‑150 (MQ=255)

                                                                                         aCCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGaa                                                        <  1:2060997/150‑1 (MQ=255)

                                                                                          ccGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAg                                                       <  1:174741/150‑1 (MQ=255)

                                                                                            ggCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGt                                                     <  2:2693267/150‑1 (MQ=255)

                                                                                            ggCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGt                                                     >  2:2517228/1‑150 (MQ=255)

                                                                                                 taGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAggg                                                <  1:1801300/150‑1 (MQ=255)

                                                                                                 taGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAggg                                                <  2:879527/150‑1 (MQ=255)

                                                                                                    ccgccAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGgaga                                             <  1:2643776/150‑1 (MQ=255)

                                                                                                         aGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAgcgc                                        >  2:2958072/1‑150 (MQ=255)

                                                                                                           aCCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCt                                      >  1:670265/1‑150 (MQ=255)

                                                                                                           aCCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCt                                      <  1:2489938/150‑1 (MQ=255)

                                                                                                           aCCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCt                                      <  1:2891195/150‑1 (MQ=255)

                                                                                                           aCCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCt                                      <  1:3105823/150‑1 (MQ=255)

                                                                                                            ccATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTg                                     >  1:1786061/1‑150 (MQ=255)

                                                                                                             cATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGc                                    >  2:3209746/1‑150 (MQ=255)

                                                                                                             cATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGc                                    >  2:3209794/1‑150 (MQ=255)

                                                                                                              aTTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGcc                                   >  1:2962037/1‑150 (MQ=255)

                                                                                                               ttAATAAATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGccc                                  >  2:2423719/1‑150 (MQ=255)

                                                                                                                taataaATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCt                                 >  2:1487846/1‑150 (MQ=255)

                                                                                                                 aataaATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCtt                                <  1:2466556/150‑1 (MQ=255)

                                                                                                                 aataaATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGACAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCtt                                >  2:2307249/1‑150 (MQ=255)

                                                                                                                   taaATGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTc                              >  1:1353539/1‑150 (MQ=255)

                                                                                                                    aaaTGGGTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCt                             <  1:434306/150‑1 (MQ=255)

                                                                                                                         ggTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGa                        >  1:3437939/1‑150 (MQ=255)

                                                                                                                          gTACGCAAGGAAGGCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGAt                       <  1:328970/150‑1 (MQ=255)

                                                                                                                                     aggCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGa            >  2:2581259/1‑150 (MQ=255)

                                                                                                                                      ggCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGaa           <  2:3021989/150‑1 (MQ=255)

                                                                                                                                       gCTGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAg          >  1:195321/1‑150 (MQ=255)

                                                                                                                                         tGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTa        >  2:2007605/1‑150 (MQ=255)

                                                                                                                                         tGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTa        >  2:524163/1‑150 (MQ=255)

                                                                                                                                         tGGACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTa        >  1:1191882/1‑150 (MQ=255)

                                                                                                                                           gACAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTAtt      <  2:2889011/150‑1 (MQ=255)

                                                                                                                                            aCAACGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTg     <  2:1515751/150‑1 (MQ=255)

                                                                                                                                               aCGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg  >  2:1223384/1‑150 (MQ=255)

                                                                                                                                               aCGTAACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg  <  1:929377/150‑1 (MQ=255)

TTATAAAATGAACTACTAATAGACCCACATACATTCAGGGAATTGTTATGTTCAAGGAGAGGATGACACCAGATGAACTTGCCAGACTGACCGGCTATAGCCGCCAGACCATTAATAAATGGGTACGCAAGGAAGGCTGGACAACGTCACCAAAACCTGGCGTCCAGGGTGGCAAAGCCCGACTGGTTCACGTCAATGAACAGGTTCGTGAATATATTCGCAATGCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTG  >  CP009273/2511884‑2512176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`