Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,542,627 T→G I389M (ATT→ATG)  yfeW → penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,542,6270TG100.0% 1056.5 / NA 266I389M (ATT→ATG) yfeWpenicillin binding protein PBP4B; weak DD‑carboxypeptidase activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (94/172);  total (94/172)

Only 100 of 271 total aligned reads displayed.

AGATGCCACTTTTGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATTGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGG  >  CP009273/2542480‑2542773
                                                                                                                                                   |                                                                                                                                                  
aGATGCCACTTTTGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATggt                                                                                                                                                  <  1:131530/150‑1 (MQ=255)
 gATGCCACTTTTGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATggtg                                                                                                                                                 <  1:474518/150‑1 (MQ=255)
   tGCCACTTTTGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGAt                                                                                                                                               <  1:1680357/150‑1 (MQ=255)
     ccACTTTTGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGt                                                                                                                                             <  2:3594682/150‑1 (MQ=255)
     ccACTTTTGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGt                                                                                                                                             <  2:3639624/150‑1 (MQ=255)
       aCTTTTGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTa                                                                                                                                           >  2:3602998/1‑150 (MQ=255)
         ttttGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAg                                                                                                                                         <  1:2721571/150‑1 (MQ=255)
           ttGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGca                                                                                                                                       <  1:1089209/150‑1 (MQ=255)
           ttGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGca                                                                                                                                       <  1:2211144/150‑1 (MQ=255)
           ttGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGca                                                                                                                                       <  2:3040335/150‑1 (MQ=255)
            tGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGcaa                                                                                                                                      >  1:2508718/1‑150 (MQ=255)
            tGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGcaa                                                                                                                                      >  1:3419194/1‑150 (MQ=255)
              gCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGcaaca                                                                                                                                    <  2:1757363/150‑1 (MQ=255)
                cTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAg                                                                                                                                  <  1:3212453/150‑1 (MQ=255)
                cTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAg                                                                                                                                  <  2:1691811/150‑1 (MQ=255)
                cTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAg                                                                                                                                  <  2:3386629/150‑1 (MQ=255)
                  cGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGcc                                                                                                                                <  1:3007077/150‑1 (MQ=255)
                    gCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCcac                                                                                                                              <  2:2614284/150‑1 (MQ=255)
                       ggCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACAtt                                                                                                                           <  1:1265392/150‑1 (MQ=255)
                        gcgcGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTc                                                                                                                          <  2:3743657/150‑1 (MQ=255)
                        gcgcGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTc                                                                                                                          <  2:1947812/150‑1 (MQ=255)
                        gcgcGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTc                                                                                                                          <  2:152677/150‑1 (MQ=255)
                          gcgTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGc                                                                                                                        <  1:3097626/150‑1 (MQ=255)
                            gTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCg                                                                                                                      <  2:913316/150‑1 (MQ=255)
                            gTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCg                                                                                                                      <  2:679199/150‑1 (MQ=255)
                             tGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCgg                                                                                                                     <  1:1625929/150‑1 (MQ=255)
                                aTGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTg                                                                                                                  <  1:2358416/150‑1 (MQ=255)
                                aTGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTg                                                                                                                  <  1:2312624/150‑1 (MQ=255)
                                  ggTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGcc                                                                                                                <  2:435571/150‑1 (MQ=255)
                                  ggTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGcc                                                                                                                <  1:2418479/150‑1 (MQ=255)
                                  ggTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGcc                                                                                                                <  1:1331488/150‑1 (MQ=255)
                                  ggTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGcc                                                                                                                <  2:1430938/150‑1 (MQ=255)
                                     aaTGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGAt                                                                                                             <  2:3145518/150‑1 (MQ=255)
                                       tGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATcc                                                                                                           <  2:1912083/150‑1 (MQ=255)
                                          caccaTGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCa                                                                                                        >  1:3639589/1‑150 (MQ=255)
                                              aTGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAg                                                                                                    <  2:1041265/150‑1 (MQ=255)
                                                 aCGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAAt                                                                                                 >  1:722998/1‑150 (MQ=255)
                                                   gCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATcc                                                                                               <  2:2234937/150‑1 (MQ=255)
                                                        cGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAata                                                                                          <  1:2281489/150‑1 (MQ=255)
                                                         gTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAatat                                                                                         >  1:3375764/1‑150 (MQ=255)
                                                          tttGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATg                                                                                        >  1:2551164/1‑150 (MQ=255)
                                                            tGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGtt                                                                                      <  1:861958/150‑1 (MQ=255)
                                                             ggCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTc                                                                                     <  2:2541523/150‑1 (MQ=255)
                                                             ggCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTc                                                                                     <  2:2985023/150‑1 (MQ=255)
                                                                   cTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGc                                                                               <  2:3435849/150‑1 (MQ=255)
                                                                   cTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGc                                                                               <  1:1254162/150‑1 (MQ=255)
                                                                      gCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGt                                                                            <  2:3083967/150‑1 (MQ=255)
                                                                      gCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGt                                                                            <  2:1782337/150‑1 (MQ=255)
                                                                        aaGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCa                                                                          <  2:2450172/150‑1 (MQ=255)
                                                                        aaGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCa                                                                          <  1:3240053/150‑1 (MQ=255)
                                                                         aGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAg                                                                         >  2:2042534/1‑150 (MQ=255)
                                                                          gCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGt                                                                        <  1:1130396/150‑1 (MQ=255)
                                                                           cccGCATTCCTACGGTTTCTCTGGTTGGATCGGTTCGGTGAAAGTTATCGATCTGGTGTATCATATGACGATGGTGATGTTAATCAAAAAGCAAAATTCGCCGGTTTCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGtt                                                                       <  1:1601407/150‑1 (MQ=255)
                                                                            ccGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTg                                                                      >  2:2143074/1‑150 (MQ=255)
                                                                             cGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGc                                                                     >  1:528050/1‑150 (MQ=255)
                                                                              gCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGcc                                                                    <  1:2907574/150‑1 (MQ=255)
                                                                                aaaCCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGa                                                                  >  2:582488/1‑150 (MQ=255)
                                                                                  aCCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGAtt                                                                <  2:1158093/150‑1 (MQ=255)
                                                                                  aCCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGAtt                                                                >  1:344602/1‑150 (MQ=255)
                                                                                    cTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGc                                                              >  1:3281701/1‑150 (MQ=255)
                                                                                    cTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGc                                                              <  1:3602998/150‑1 (MQ=255)
                                                                                     tACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCa                                                             >  1:1453554/1‑150 (MQ=255)
                                                                                     tACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCa                                                             >  1:2649753/1‑150 (MQ=255)
                                                                                        ggTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACt                                                          <  2:1285541/150‑1 (MQ=255)
                                                                                        ggTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACt                                                          <  2:3186677/150‑1 (MQ=255)
                                                                                         gTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACtt                                                         <  2:1823139/150‑1 (MQ=255)
                                                                                          tCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTa                                                        >  1:1060113/1‑150 (MQ=255)
                                                                                          tCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTa                                                        >  1:202832/1‑150 (MQ=255)
                                                                                               ctggctggACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGtt                                                   <  2:3385101/150‑1 (MQ=255)
                                                                                               ctggctggACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGtt                                                   <  2:2772657/150‑1 (MQ=255)
                                                                                                  gctggACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTggg                                                >  2:2351781/1‑150 (MQ=255)
                                                                                                    tggACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTa                                              >  1:1786113/1‑150 (MQ=255)
                                                                                                     ggACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAg                                             >  2:2895633/1‑150 (MQ=255)
                                                                                                      gACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGt                                            <  2:3722881/150‑1 (MQ=255)
                                                                                                        ccGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTg                                          <  2:1978678/150‑1 (MQ=255)
                                                                                                          ggAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGAt                                        <  1:1936370/150‑1 (MQ=255)
                                                                                                           gAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATc                                       <  2:1624788/150‑1 (MQ=255)
                                                                                                              cGGTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAgg                                    <  1:1833585/150‑1 (MQ=255)
                                                                                                               ggTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGgt                                   <  1:1863670/150‑1 (MQ=255)
                                                                                                                gTGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGgtg                                  <  1:3805157/150‑1 (MQ=255)
                                                                                                                 tGACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGgtgt                                 <  2:2468011/150‑1 (MQ=255)
                                                                                                                  gACCGTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTa                                >  1:199580/1‑150 (MQ=255)
                                                                                                                      gTTATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATgcg                            >  2:1633938/1‑150 (MQ=255)
                                                                                                                       ttATCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATgcgg                           >  2:1056470/1‑150 (MQ=255)
                                                                                                                         aTCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATgcggcg                         >  2:2228335/1‑150 (MQ=255)
                                                                                                                         aTCGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATgcggcg                         <  2:2929225/150‑1 (MQ=255)
                                                                                                                           cGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGtt                       <  1:582488/150‑1 (MQ=255)
                                                                                                                           cGATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGtt                       <  2:1667136/150‑1 (MQ=255)
                                                                                                                            gATCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTa                      <  1:2925247/150‑1 (MQ=255)
                                                                                                                              tCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTaaa                    >  2:3286590/1‑150 (MQ=255)
                                                                                                                              tCCGGTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTAGGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTaaa                    >  2:2733132/1‑150 (MQ=255)
                                                                                                                                 ggTGAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCa                 <  2:2530267/150‑1 (MQ=255)
                                                                                                                                    gAATCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGaa              >  1:2164616/1‑150 (MQ=255)
                                                                                                                                       tCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGta           <  2:1392861/150‑1 (MQ=255)
                                                                                                                                       tCATATGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGta           >  2:2496866/1‑150 (MQ=255)
                                                                                                                                         atatGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGtaat         <  1:3652222/150‑1 (MQ=255)
                                                                                                                                          tatGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGtaata        >  2:785455/1‑150 (MQ=255)
                                                                                                                                           atGACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGtaataa       <  1:1043173/150‑1 (MQ=255)
                                                                                                                                             gACGATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGGTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATaaaa     >  2:1572279/1‑150 (MQ=255)
                                                                                                                                                gATGGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAgg  <  1:2706494/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGATGCCACTTTTGGCCTCGGCTGGCGCGTGAATGGTAATGCCACCATGACGCCGACGTTTGGCACGCTGGCAAGCCCGCAAACCTACGGTCACACTGGCTGGACCGGAACGGTGACCGTTATCGATCCGGTGAATCATATGACGATTGTGATGTTAAGCAACAAGCCACATTCGCCGGTTGCCGATCCGCAAAAGAATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGG  >  CP009273/2542480‑2542773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑