Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,546,198 T→G L163R (CTG→CGG)  amiA → N‑acetylmuramoyl‑l‑alanine amidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,546,1980TG100.0% 1047.4 / NA 264L163R (CTG→CGG) amiAN‑acetylmuramoyl‑l‑alanine amidase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (126/138);  total (126/138)

Only 100 of 264 total aligned reads displayed.

TTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACGCTCGGCTCGCATATTCTGAAGAAGA  >  CP009273/2546052‑2546344
                                                                                                                                                  |                                                                                                                                                  
 tACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCt                                                                                                                                                <  1:1003565/150‑1 (MQ=255)
  aCGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTg                                                                                                                                               >  2:2226508/1‑150 (MQ=255)
   cGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGa                                                                                                                                              >  1:510386/1‑150 (MQ=255)
   cGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGa                                                                                                                                              <  2:2939311/150‑1 (MQ=255)
       cgcgTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAAcgc                                                                                                                                          >  2:3350860/1‑150 (MQ=255)
           ttGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGaaa                                                                                                                                      >  2:3065353/1‑150 (MQ=255)
           ttGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGaaa                                                                                                                                      <  1:3070445/150‑1 (MQ=255)
            tGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGaaaa                                                                                                                                     >  2:824116/1‑150 (MQ=255)
             gAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAAc                                                                                                                                    >  1:1212348/1‑150 (MQ=255)
              aaaTCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAAcc                                                                                                                                   >  2:1337285/1‑150 (MQ=255)
               aaTCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACcg                                                                                                                                  >  2:2013183/1‑150 (MQ=255)
               aaTCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACcg                                                                                                                                  <  2:1646694/150‑1 (MQ=255)
                   gCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGcc                                                                                                                              <  1:2172355/150‑1 (MQ=255)
                    cccATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCGAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCg                                                                                                                             >  1:3601512/1‑150 (MQ=255)
                     ccATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGa                                                                                                                            >  2:1919429/1‑150 (MQ=255)
                      cATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGAt                                                                                                                           <  2:722745/150‑1 (MQ=255)
                      cATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGAt                                                                                                                           <  2:25519/150‑1 (MQ=255)
                       aTAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATg                                                                                                                          >  2:1007799/1‑150 (MQ=255)
                          aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAg                                                                                                                       >  2:1765366/1‑150 (MQ=255)
                           aCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGt                                                                                                                      <  1:3582626/150‑1 (MQ=255)
                           aCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGt                                                                                                                      <  2:3250073/150‑1 (MQ=255)
                             aTGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTg                                                                                                                    >  1:81997/1‑150 (MQ=255)
                                    aGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTaa                                                                                                             >  2:3618427/1‑150 (MQ=255)
                                      aTCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTaaaa                                                                                                           >  2:456097/1‑150 (MQ=255)
                                      aTCTGTTAATGTCAATTCATGCCGATGGCTATAACAACACGAAATCTGCCGGTGCTTCGTTATTTGCCCTCTCTAACCGTGGGTAAAGTAGCGCAATAGCTAAATACCGATCTGAACACGAAAACATCGCCGATAAATTTGCCGGTaaaa                                                                                                           >  1:244971/1‑150 (MQ=255)
                                       tCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTaaaaa                                                                                                          <  2:1087702/150‑1 (MQ=255)
                                          gTTTATGTCATTTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCTAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGc                                                                                                       <  2:3783030/150‑1 (MQ=255)
                                          gTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGc                                                                                                       <  1:2029884/150‑1 (MQ=255)
                                           tttATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCg                                                                                                      >  1:436533/1‑150 (MQ=255)
                                               tGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTg                                                                                                  <  2:2533636/150‑1 (MQ=255)
                                                gTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGa                                                                                                 >  2:743538/1‑150 (MQ=255)
                                                 tCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGAc                                                                                                <  1:1901182/150‑1 (MQ=255)
                                                 tCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGAc                                                                                                <  1:614090/150‑1 (MQ=255)
                                                  cAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACa                                                                                               <  1:695695/150‑1 (MQ=255)
                                                   aaTTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCACTGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACaa                                                                                              >  2:1787756/1‑150 (MQ=255)
                                                   aaTTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACaa                                                                                              >  1:3746487/1‑150 (MQ=255)
                                                    aTTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAg                                                                                             <  2:340181/150‑1 (MQ=255)
                                                     ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAgg                                                                                            <  2:1375470/150‑1 (MQ=255)
                                                     ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAgg                                                                                            <  1:2598544/150‑1 (MQ=255)
                                                     ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAgg                                                                                            <  2:2199910/150‑1 (MQ=255)
                                                     ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAgg                                                                                            <  2:2774985/150‑1 (MQ=255)
                                                       cATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGAt                                                                                          <  2:3641345/150‑1 (MQ=255)
                                                        aTGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATc                                                                                         <  1:3780823/150‑1 (MQ=255)
                                                        aTGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATc                                                                                         >  1:786727/1‑150 (MQ=255)
                                                        aTGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATc                                                                                         >  2:190331/1‑150 (MQ=255)
                                                        aTGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATc                                                                                         <  1:2220944/150‑1 (MQ=255)
                                                         tGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCa                                                                                        <  2:2455818/150‑1 (MQ=255)
                                                          gCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGGTGCCGGTAAAAAGGCGACTGACAAGGATCAc                                                                                       <  1:3155056/150‑1 (MQ=255)
                                                            cGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCt                                                                                     >  2:1462727/1‑150 (MQ=255)
                                                             gATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTa                                                                                    >  1:2055092/1‑150 (MQ=255)
                                                             gATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTa                                                                                    >  1:3545564/1‑150 (MQ=255)
                                                              aTGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTAt                                                                                   >  1:2600727/1‑150 (MQ=255)
                                                               tGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTAtt                                                                                  <  2:3544132/150‑1 (MQ=255)
                                                                ggCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTg                                                                                 >  1:1374612/1‑150 (MQ=255)
                                                                ggCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTg                                                                                 <  2:2118039/150‑1 (MQ=255)
                                                                   tttACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGcaa                                                                              >  1:1922612/1‑150 (MQ=255)
                                                                    ttACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGcaac                                                                             >  2:1326332/1‑150 (MQ=255)
                                                                         aaCCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTg                                                                        >  1:3021923/1‑150 (MQ=255)
                                                                            ccGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTg                                                                     <  1:3058483/150‑1 (MQ=255)
                                                                            ccGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTg                                                                     <  1:651193/150‑1 (MQ=255)
                                                                              gAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGtt                                                                   <  1:1765366/150‑1 (MQ=255)
                                                                              gAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGtt                                                                   >  2:2111257/1‑150 (MQ=255)
                                                                              gAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGtt                                                                   <  1:961891/150‑1 (MQ=255)
                                                                              gAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGtt                                                                   >  2:1664400/1‑150 (MQ=255)
                                                                              gAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGtt                                                                   >  2:953941/1‑150 (MQ=255)
                                                                              gAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGtt                                                                   >  1:2730396/1‑150 (MQ=255)
                                                                              gAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGtt                                                                   >  2:1512781/1‑150 (MQ=255)
                                                                               aaaGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGttt                                                                  <  2:2913217/150‑1 (MQ=255)
                                                                               aaaGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGttt                                                                  <  1:2491004/150‑1 (MQ=255)
                                                                                   cTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGACGATGAAGTTGCCGATAAAAAGACGACTAACAAGGATCACCTATTGCAACAAATGCTGTTTGATc                                                              >  1:2869851/1‑150 (MQ=255)
                                                                                    tGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCt                                                             <  2:3647258/150‑1 (MQ=255)
                                                                                      ccGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTgg                                                           <  1:1016236/150‑1 (MQ=255)
                                                                                      ccGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTgg                                                           <  1:158775/150‑1 (MQ=255)
                                                                                        ggTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTg                                                         <  2:1363108/150‑1 (MQ=255)
                                                                                        ggTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCGATTGCAACAAGTGCTGTTTGATCTGGTg                                                         >  2:538593/1‑150 (MQ=255)
                                                                                         gTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGc                                                        <  2:3593415/150‑1 (MQ=255)
                                                                                            cTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCaaa                                                     >  1:3711474/1‑150 (MQ=255)
                                                                                             ttCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAAc                                                    <  1:153450/150‑1 (MQ=255)
                                                                                              tCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACa                                                   <  2:2854044/150‑1 (MQ=255)
                                                                                              tCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACa                                                   <  1:594150/150‑1 (MQ=255)
                                                                                               cGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAg                                                  <  1:944113/150‑1 (MQ=255)
                                                                                                ggTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGa                                                 <  2:2290246/150‑1 (MQ=255)
                                                                                                 gTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGAt                                                >  2:1937551/1‑150 (MQ=255)
                                                                                                      tGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCAt                                           <  1:2783430/150‑1 (MQ=255)
                                                                                                          ctctctAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTaaa                                       >  2:497506/1‑150 (MQ=255)
                                                                                                            ctctAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTaaaaa                                     >  1:1443449/1‑150 (MQ=255)
                                                                                                             tctAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAAt                                    <  2:932836/150‑1 (MQ=255)
                                                                                                                aaCCGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGt                                 <  1:2442898/150‑1 (MQ=255)
                                                                                                                  ccGTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCt                               <  1:3198600/150‑1 (MQ=255)
                                                                                                                    gTGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGa                             <  2:2753303/150‑1 (MQ=255)
                                                                                                                     tGGGGCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGAc                            <  2:881198/150‑1 (MQ=255)
                                                                                                                        ggCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACgct                         >  1:2533470/1‑150 (MQ=255)
                                                                                                                         gCAAGTAGCGCAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACgctc                        >  1:1964333/1‑150 (MQ=255)
                                                                                                                             gTAGCGCAATGGCGAAATACCGGTCTGAACGCGAACACCGCGCCGTTGTAGTGGCCGGTAAAAAGGCGACTGACCAGGATCTCCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAAGAGTCTGACgctcggct                    <  2:2665844/150‑1 (MQ=255)
                                                                                                                                  gcAATGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATAGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACGCTCGGCTCGCat               >  2:1730157/1‑150 (MQ=255)
                                                                                                                                    aaTGGCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACGCTCGGCTCGCatat             >  2:3473450/1‑150 (MQ=255)
                                                                                                                                        gCGAAATACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACGCTCGGCTCGCATATTCTg         <  1:3401563/150‑1 (MQ=255)
                                                                                                                                           aaaTACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACGCTCGGCTCGCATATTCTgaag      >  2:1481371/1‑150 (MQ=255)
                                                                                                                                             aTACCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACGCTCGGCTCGCATATTCTgaagaa    >  2:2646985/1‑150 (MQ=255)
                                                                                                                                               aCCGGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACGCTCGGCTCGCATATTCTGAagaaga  >  2:2446331/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTGTTTGATCTGGTGCAAACAGATACCATTAAAAATAGTCTGACGCTCGGCTCGCATATTCTGAAGAAGA  >  CP009273/2546052‑2546344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑