Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,596,168 C→A P331Q (CCG→CAG)  hyfB → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,596,1680CA100.0% 677.8 / NA 204P331Q (CCG→CAG) hyfBhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (100/104);  total (100/104)

Only 100 of 204 total aligned reads displayed.

TCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGAAAC  >  CP009273/2596021‑2596314
                                                                                                                                                   |                                                                                                                                                  
tCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAgc                                                                                                                                                  >  2:1718533/1‑150 (MQ=255)
 cGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAgct                                                                                                                                                 >  1:2616937/1‑150 (MQ=255)
 cGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAgct                                                                                                                                                 >  1:3332614/1‑150 (MQ=255)
 cGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAgct                                                                                                                                                 <  2:3727098/150‑1 (MQ=255)
     gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTc                                                                                                                                             >  1:3176919/1‑150 (MQ=255)
     gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTc                                                                                                                                             <  2:18360/150‑1 (MQ=255)
     gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTc                                                                                                                                             >  1:2401175/1‑150 (MQ=255)
     gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTc                                                                                                                                             <  1:2773772/150‑1 (MQ=255)
     gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTc                                                                                                                                             >  1:2400447/1‑150 (MQ=255)
           tcCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTg                                                                                                                                       >  2:3504059/1‑150 (MQ=255)
                   ccTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGACGGCATTATTTTGCTGGCAGTCTGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACt                                                                                                                               >  1:3157066/1‑150 (MQ=255)
                        gCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCg                                                                                                                          <  2:2483214/150‑1 (MQ=255)
                           tGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGgcg                                                                                                                       >  2:960885/1‑150 (MQ=255)
                               atatGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACt                                                                                                                   <  2:3190503/150‑1 (MQ=255)
                               atatGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGACGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACt                                                                                                                   >  1:2324854/1‑150 (MQ=255)
                                   gcgcTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGttt                                                                                                               <  2:2057787/150‑1 (MQ=255)
                                       tGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATc                                                                                                           >  2:776904/1‑150 (MQ=255)
                                        ggCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCt                                                                                                          <  2:3635791/150‑1 (MQ=255)
                                        ggCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCt                                                                                                          >  1:1490994/1‑150 (MQ=255)
                                         gCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTg                                                                                                         <  1:208482/150‑1 (MQ=255)
                                          cGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGc                                                                                                        <  1:1608846/150‑1 (MQ=255)
                                                aGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAAcc                                                                                                  <  2:24187/150‑1 (MQ=255)
                                                 ggATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCa                                                                                                 <  1:923618/150‑1 (MQ=255)
                                                   atatCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATg                                                                                               >  2:1784655/1‑150 (MQ=255)
                                                    tatCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATgc                                                                                              >  1:154393/1‑150 (MQ=255)
                                                     atCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATgcg                                                                                             >  1:1374129/1‑150 (MQ=255)
                                                       cAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCt                                                                                           <  1:3726028/150‑1 (MQ=255)
                                                       cAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCt                                                                                           <  1:3692434/150‑1 (MQ=255)
                                                       cAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCt                                                                                           <  1:1793938/150‑1 (MQ=255)
                                                        aaaCGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTg                                                                                          >  2:3227142/1‑150 (MQ=255)
                                                         aaCGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGt                                                                                         >  1:1098767/1‑150 (MQ=255)
                                                          aCGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGtt                                                                                        >  2:497340/1‑150 (MQ=255)
                                                           cGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCCGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTc                                                                                       <  1:3289821/150‑1 (MQ=255)
                                                            ggCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCa                                                                                      <  1:1239804/150‑1 (MQ=255)
                                                            ggCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGACGGTGTGGCGATGGTCGGTCTGTCACTGCACAACCAGCTGCTCACCGTAGTTGGACTGCTCGGCGCACTATTTCATCTGCTCAACCATGCGCTGTTCa                                                                                      >  1:3507723/1‑150 (MQ=255)
                                                              ctgctgGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCaaa                                                                                    >  2:820938/1‑150 (MQ=255)
                                                              ctgctgGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCaaa                                                                                    >  2:435002/1‑150 (MQ=255)
                                                                gctgGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAgg                                                                                  >  2:3037528/1‑150 (MQ=255)
                                                                gctgGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAgg                                                                                  >  2:3577759/1‑150 (MQ=255)
                                                                 ctgGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAggg                                                                                 >  1:6092/1‑150 (MQ=255)
                                                                   ggCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGgct                                                                               <  1:3615439/150‑1 (MQ=255)
                                                                    gCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGgctg                                                                              <  2:3048012/150‑1 (MQ=255)
                                                                     ccTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGgctgc                                                                             >  1:190777/1‑150 (MQ=255)
                                                                       tGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTa                                                                           <  2:3138744/150‑1 (MQ=255)
                                                                       tGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTa                                                                           <  2:341989/150‑1 (MQ=255)
                                                                       tGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTa                                                                           <  2:2248836/150‑1 (MQ=255)
                                                                       tGGAGGACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTa                                                                           <  2:961870/150‑1 (MQ=255)
                                                                         gAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTAtt                                                                         <  2:1325351/150‑1 (MQ=255)
                                                                          aGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTAttt                                                                        >  2:840327/1‑150 (MQ=255)
                                                                           gTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTtc                                                                       >  1:3100621/1‑150 (MQ=255)
                                                                           gTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTtc                                                                       <  2:2972828/150‑1 (MQ=255)
                                                                            tACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTtct                                                                      >  2:1661201/1‑150 (MQ=255)
                                                                            tACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTtct                                                                      >  1:1180096/1‑150 (MQ=255)
                                                                             aCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTtctc                                                                     >  1:2227359/1‑150 (MQ=255)
                                                                               cGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCgg                                                                   <  2:2062479/150‑1 (MQ=255)
                                                                               cGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCgg                                                                   <  2:2336214/150‑1 (MQ=255)
                                                                                gTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGgc                                                                  >  1:3257826/1‑150 (MQ=255)
                                                                                 tCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGgcg                                                                 <  1:2649319/150‑1 (MQ=255)
                                                                                 tCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGgcg                                                                 <  1:2432552/150‑1 (MQ=255)
                                                                                 tCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGgcg                                                                 <  2:1887747/150‑1 (MQ=255)
                                                                                 tCGAAAACGTCGGCAATATATAGCTGGCAGTCGGTGAGGCGAAGGACGGACAGTCACAGAACGACCAGCAGCTCACCGTGGTTGGACTGCTAGGCGCACTGTATCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGgcg                                                                 >  2:226127/1‑150 (MQ=255)
                                                                                   gAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGcgcg                                                               >  2:3245356/1‑150 (MQ=255)
                                                                                     aaaCGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCggg                                                             >  2:103358/1‑150 (MQ=255)
                                                                                      aaCGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGa                                                            >  2:579817/1‑150 (MQ=255)
                                                                                      aaCGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGa                                                            >  2:253944/1‑150 (MQ=255)
                                                                                       aCGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAg                                                           <  2:1165587/150‑1 (MQ=255)
                                                                                         gTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCTCTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCg                                                         <  1:803758/150‑1 (MQ=255)
                                                                                         gTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCg                                                         <  1:1460752/150‑1 (MQ=255)
                                                                                           cGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCTCGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGat                                                       <  2:1476387/150‑1 (MQ=255)
                                                                                           cGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGat                                                       >  1:2261180/1‑150 (MQ=255)
                                                                                             gCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGatta                                                     <  2:2495499/150‑1 (MQ=255)
                                                                                              cATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGattat                                                    <  1:2883979/150‑1 (MQ=255)
                                                                                                  attTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTcg                                                >  2:2154336/1‑150 (MQ=255)
                                                                                                   ttttGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTcgc                                               >  1:911426/1‑150 (MQ=255)
                                                                                                    tttGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTcgcg                                              <  1:2748601/150‑1 (MQ=255)
                                                                                                       gCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGttt                                           <  2:2300064/150‑1 (MQ=255)
                                                                                                        cTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTg                                          >  1:762503/1‑150 (MQ=255)
                                                                                                        cTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTg                                          <  1:2406710/150‑1 (MQ=255)
                                                                                                        cTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTg                                          >  1:762166/1‑150 (MQ=255)
                                                                                                        cTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTg                                          <  2:2603874/150‑1 (MQ=255)
                                                                                                          ggCAGTCGGTGGGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCa                                        <  2:2832190/150‑1 (MQ=255)
                                                                                                           gCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCAt                                       >  2:567522/1‑150 (MQ=255)
                                                                                                             aGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATAc                                     >  2:181421/1‑150 (MQ=255)
                                                                                                                cGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCa                                  <  1:100486/150‑1 (MQ=255)
                                                                                                                    gtgGCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGAc                              <  1:484272/150‑1 (MQ=255)
                                                                                                                      ggCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGAGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACAt                            >  2:3569775/1‑150 (MQ=255)
                                                                                                                       gCGATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATg                           >  1:2099780/1‑150 (MQ=255)
                                                                                                                         gATGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGa                         >  1:1728464/1‑150 (MQ=255)
                                                                                                                           tGGTCGGTCTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGaaa                       <  1:125683/150‑1 (MQ=255)
                                                                                                                            ggtcggtcTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGaaaa                      >  2:107810/1‑150 (MQ=255)
                                                                                                                                   cTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATgggg               >  2:126593/1‑150 (MQ=255)
                                                                                                                                   cTGTCACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATgggg               >  2:127380/1‑150 (MQ=255)
                                                                                                                                       cACTGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCAc           <  2:2677228/150‑1 (MQ=255)
                                                                                                                                          tGCACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAg        <  2:1211700/150‑1 (MQ=255)
                                                                                                                                            cACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCg      >  1:1649478/1‑150 (MQ=255)
                                                                                                                                            cACGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCg      >  2:3331676/1‑150 (MQ=255)
                                                                                                                                              ctaCCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGaa    >  2:3758658/3‑150 (MQ=255)
                                                                                                                                              cGACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGaa    >  1:580076/1‑150 (MQ=255)
                                                                                                                                               gACCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGaaa   >  1:1070368/1‑150 (MQ=255)
                                                                                                                                                aCCAGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGAAAc  <  1:3037528/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGAAAC  >  CP009273/2596021‑2596314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑