Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,643,336 C→A D771Y (GAC→TAC)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,643,3360CA100.0% 655.4 / NA 196D771Y (GAC→TAC) yfhMbacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (112/84);  total (112/84)

Only 100 of 196 total aligned reads displayed.

GATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGA  >  CP009273/2643189‑2643481
                                                                                                                                                   |                                                                                                                                                 
gATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGc                                                                                                                                                 >  2:141302/1‑150 (MQ=255)
 aTTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGcc                                                                                                                                                >  2:956895/1‑150 (MQ=255)
 aTTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGcc                                                                                                                                                >  2:123914/1‑150 (MQ=255)
   tCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAg                                                                                                                                              <  1:2537913/150‑1 (MQ=255)
    ccAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAgg                                                                                                                                             >  2:3191414/1‑150 (MQ=255)
     cAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAggg                                                                                                                                            >  1:1550987/1‑150 (MQ=255)
      aGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGc                                                                                                                                           >  1:528874/1‑150 (MQ=255)
      aGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGc                                                                                                                                           <  2:812193/150‑1 (MQ=255)
      aGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGc                                                                                                                                           >  1:1907115/1‑150 (MQ=255)
       ggTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCg                                                                                                                                          <  1:1110647/150‑1 (MQ=255)
       ggTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCg                                                                                                                                          <  1:1443358/150‑1 (MQ=255)
         ttttATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGAt                                                                                                                                        >  2:1360160/1‑150 (MQ=255)
           ttATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATaa                                                                                                                                      >  2:2281456/1‑150 (MQ=255)
            tATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAAc                                                                                                                                     <  1:19712/150‑1 (MQ=255)
             aTCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACt                                                                                                                                    >  2:2169274/1‑150 (MQ=255)
              tCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTg                                                                                                                                   <  2:572547/150‑1 (MQ=255)
              tCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTg                                                                                                                                   >  1:2375567/1‑150 (MQ=255)
                 aCCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCt                                                                                                                                >  1:1847330/1‑150 (MQ=255)
                    ggAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTa                                                                                                                             <  2:3490892/150‑1 (MQ=255)
                      aaTCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATc                                                                                                                           >  1:2973521/1‑150 (MQ=255)
                       aTCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATnc                                                                                                                          <  1:2308179/150‑1 (MQ=255)
                          gTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGt                                                                                                                       <  2:1642987/150‑1 (MQ=255)
                            cAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGttt                                                                                                                     >  1:2052723/1‑150 (MQ=255)
                              gATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCa                                                                                                                   >  1:42011/1‑150 (MQ=255)
                                 ccAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCg                                                                                                                <  2:2267201/150‑1 (MQ=255)
                                  cAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGt                                                                                                               >  1:1035806/1‑150 (MQ=255)
                                     cccTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGac                                                                                                            <  1:3311438/150‑1 (MQ=255)
                                          gAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggt                                                                                                       <  1:1734828/150‑1 (MQ=255)
                                          gAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggt                                                                                                       <  2:2011827/150‑1 (MQ=255)
                                           aGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggtc                                                                                                      <  2:1372431/150‑1 (MQ=255)
                                            gCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggtcg                                                                                                     <  1:2246477/150‑1 (MQ=255)
                                             cccGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggtcgg                                                                                                    >  1:2129789/1‑150 (MQ=255)
                                             cccGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggtcgg                                                                                                    >  2:3253858/1‑150 (MQ=255)
                                             cccGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggtcgg                                                                                                    >  2:2394818/1‑150 (MQ=255)
                                             cccGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggtcgg                                                                                                    >  2:728359/1‑150 (MQ=255)
                                                  aCATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGc                                                                                               >  2:906928/1‑150 (MQ=255)
                                                  aCATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGc                                                                                               >  2:2373334/1‑150 (MQ=255)
                                                  aCATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGc                                                                                               >  2:1481398/1‑150 (MQ=255)
                                                  aCATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGc                                                                                               >  2:3378069/1‑150 (MQ=255)
                                                     tCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACt                                                                                            <  2:1992696/150‑1 (MQ=255)
                                                     tCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACt                                                                                            <  2:468388/150‑1 (MQ=255)
                                                      cAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTg                                                                                           <  2:1335448/150‑1 (MQ=255)
                                                      cAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTg                                                                                           <  1:1155438/150‑1 (MQ=255)
                                                       aaTCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTgc                                                                                          >  1:967526/1‑150 (MQ=255)
                                                        aTCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTgcg                                                                                         >  1:2062218/1‑150 (MQ=255)
                                                             ttGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCt                                                                                    >  2:2579353/1‑150 (MQ=255)
                                                              tGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTa                                                                                   <  1:327477/150‑1 (MQ=255)
                                                                ccaccaCAGCGGCCCTTCTCTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTAcc                                                                                 <  1:3471514/150‑1 (MQ=255)
                                                                ccaccaCAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTAcc                                                                                 <  1:122957/150‑1 (MQ=255)
                                                                 caccacAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCg                                                                                >  1:3690057/1‑150 (MQ=255)
                                                                 caccacAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCg                                                                                <  1:284667/150‑1 (MQ=255)
                                                                    cacaGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCg                                                                             >  1:1091467/1‑150 (MQ=255)
                                                                     acaGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGc                                                                            >  2:770766/1‑150 (MQ=255)
                                                                      caGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCt                                                                           <  1:2021364/150‑1 (MQ=255)
                                                                         cGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCtgtt                                                                        >  2:3388741/1‑150 (MQ=255)
                                                                          ggCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATCACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCtgttg                                                                       >  2:3292421/1‑150 (MQ=255)
                                                                          ggCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCtgttg                                                                       >  1:1707316/1‑150 (MQ=255)
                                                                           gCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCtgttgt                                                                      >  1:3496532/1‑150 (MQ=255)
                                                                             ccTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTcc                                                                    >  2:244053/1‑150 (MQ=255)
                                                                             ccTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTcc                                                                    >  2:244128/1‑150 (MQ=255)
                                                                             ccTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTcc                                                                    <  2:2691045/150‑1 (MQ=255)
                                                                              cTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCt                                                                   >  2:3582366/1‑150 (MQ=255)
                                                                              cTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCt                                                                   <  2:387740/150‑1 (MQ=255)
                                                                                 cACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGcc                                                                <  2:2375567/150‑1 (MQ=255)
                                                                                  aCTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCa                                                               >  1:587775/1‑150 (MQ=255)
                                                                                        cTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCtata                                                         >  2:681582/1‑150 (MQ=255)
                                                                                         tCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATag                                                        >  1:2960613/1‑150 (MQ=255)
                                                                                         tCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATag                                                        >  1:1611948/1‑150 (MQ=255)
                                                                                         tCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATag                                                        >  1:3517955/1‑150 (MQ=255)
                                                                                            aCCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATagcca                                                     >  1:2113599/1‑150 (MQ=255)
                                                                                            aCCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATagcca                                                     <  1:3785924/150‑1 (MQ=255)
                                                                                              cATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTTTCCTGCCAGCTATaaccagc                                                   >  1:1977516/1‑150 (MQ=255)
                                                                                              cATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATagccagc                                                   >  1:2483218/1‑150 (MQ=255)
                                                                                              cATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATagccagc                                                   <  1:2391982/150‑1 (MQ=255)
                                                                                                tCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGccc                                                 <  1:3185491/150‑1 (MQ=255)
                                                                                                   cATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGa                                              <  1:3748254/150‑1 (MQ=255)
                                                                                                    aTAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGTGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCAGCCAGCTATAGCCAGCCCAGaa                                             >  2:2056241/1‑150 (MQ=255)
                                                                                                    aTAACCTTTACCCGCCGTTGGCGCGGAGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGaa                                             <  1:2650153/150‑1 (MQ=255)
                                                                                                     tAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGaaa                                            >  2:1802992/1‑150 (MQ=255)
                                                                                                      aaCCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAAc                                           >  1:2826821/1‑150 (MQ=255)
                                                                                                         cTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGaa                                        >  2:1770035/1‑150 (MQ=255)
                                                                                                                  ccgTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGa                               <  1:770766/150‑1 (MQ=255)
                                                                                                                   cgTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGAc                              <  2:3175969/150‑1 (MQ=255)
                                                                                                                    gTTGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGAcc                             >  1:3653087/1‑150 (MQ=255)
                                                                                                                     ttGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCg                            >  1:1024230/1‑150 (MQ=255)
                                                                                                                     ttGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCg                            >  2:3220001/1‑150 (MQ=255)
                                                                                                                      tGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCCGCTATAGCCAGCCCAGAAACGAACACTACTGACCGc                           >  2:1012383/1‑150 (MQ=255)
                                                                                                                      tGGCGCGGCGATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGc                           >  1:3035088/1‑150 (MQ=255)
                                                                                                                          gcggcgATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCa                       <  1:1000527/150‑1 (MQ=255)
                                                                                                                           cggcgATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCAt                      >  1:1343224/1‑150 (MQ=255)
                                                                                                                            ggcgATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCaat                     >  1:3082228/1‑148 (MQ=255)
                                                                                                                            ggcgATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCAtt                     <  2:2086641/150‑1 (MQ=255)
                                                                                                                               gATATGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCgg                  >  1:2426564/1‑150 (MQ=255)
                                                                                                                                atatGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGgc                 >  1:82329/1‑150 (MQ=255)
                                                                                                                                   tGCAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCt              <  2:562454/150‑1 (MQ=255)
                                                                                                                                     cAACTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCttt            <  2:3035088/150‑1 (MQ=255)
                                                                                                                                        cTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGAc         <  1:3625694/150‑1 (MQ=255)
                                                                                                                                        cTTAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGAc         >  2:492509/1‑150 (MQ=255)
                                                                                                                                          tAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACtt       <  1:837758/150‑1 (MQ=255)
                                                                                                                                          tAATGGTGTAGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACtt       <  1:1863633/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCGCCGCTACCGTCGCTGTTGTCCTGCCAGCTATAGCCAGCCCAGAAACGAACACTACTGACCGCTTCATTCGGCGCTTTGACTTCCAGA  >  CP009273/2643189‑2643481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑