Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,669,978 C→A A69A (GCG→GCT)  yphD ← inner membrane putative ABC superfamily sugar transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,669,9780CA99.5% 719.4 / NA 219A69A (GCG→GCT) yphDinner membrane putative ABC superfamily sugar transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (113/105);  total (113/106)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGACT  >  CP009273/2669831‑2670124
                                                                                                                                                   |                                                                                                                                                  
 aTCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGcc                                                                                                                                                 <  2:2643086/150‑1 (MQ=255)
  tCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGccc                                                                                                                                                <  2:429513/150‑1 (MQ=255)
     gcgcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAgg                                                                                                                                             <  1:1829175/150‑1 (MQ=255)
      cgcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAggc                                                                                                                                            >  2:3113534/1‑150 (MQ=255)
          ccTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCa                                                                                                                                        <  2:2498776/150‑1 (MQ=255)
            tAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAAt                                                                                                                                      >  2:272709/1‑150 (MQ=255)
            tAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAAt                                                                                                                                      >  1:2438482/1‑150 (MQ=255)
                 gCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCaa                                                                                                                                 >  2:707523/1‑150 (MQ=255)
                  cAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAt                                                                                                                                <  1:3447506/150‑1 (MQ=255)
                      acCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtggtg                                                                                                                            >  2:487867/1‑150 (MQ=255)
                      acCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtggtg                                                                                                                            >  2:2314233/1‑150 (MQ=255)
                       ccAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATACTAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtggtgg                                                                                                                           >  2:759314/1‑150 (MQ=255)
                       ccAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtggtgg                                                                                                                           <  2:416959/150‑1 (MQ=255)
                           cagGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTggcggc                                                                                                                       <  2:3400676/150‑1 (MQ=255)
                           cagGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTggcggc                                                                                                                       <  1:79244/150‑1 (MQ=255)
                               cACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATcg                                                                                                                   >  2:3682600/1‑150 (MQ=255)
                                aCGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATcgc                                                                                                                  >  2:1410450/1‑150 (MQ=255)
                                  gCCTCCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCgcgc                                                                                                                <  2:565957/150‑1 (MQ=255)
                                  gCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCgcgc                                                                                                                >  2:1696460/1‑150 (MQ=255)
                                    cACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcag                                                                                                              >  1:2054027/1‑150 (MQ=255)
                                    cACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATACCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcag                                                                                                              <  2:3323496/150‑1 (MQ=255)
                                     aCCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcagc                                                                                                             <  1:2290144/150‑1 (MQ=255)
                                       cGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCAc                                                                                                           <  2:2233786/150‑1 (MQ=255)
                                          cAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt                                                                                                        <  2:2001524/150‑1 (MQ=255)
                                          cAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt                                                                                                        >  1:266241/1‑150 (MQ=255)
                                          cAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt                                                                                                        >  2:3252840/1‑150 (MQ=255)
                                           aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTc                                                                                                       <  2:1020604/150‑1 (MQ=255)
                                           aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTc                                                                                                       >  1:3713839/1‑150 (MQ=255)
                                           aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTc                                                                                                       >  2:868890/1‑150 (MQ=255)
                                             cGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                     >  2:2975020/1‑150 (MQ=255)
                                              ggAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATc                                                                                                    >  2:1450925/1‑150 (MQ=255)
                                               gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                   >  1:1795757/1‑150 (MQ=255)
                                               gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                   <  1:167068/150‑1 (MQ=255)
                                               gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGACACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                   <  1:2077342/150‑1 (MQ=255)
                                                aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTg                                                                                                  >  2:3648835/1‑150 (MQ=255)
                                                  cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGt                                                                                                <  1:3049940/150‑1 (MQ=255)
                                                  cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGt                                                                                                <  1:3467124/150‑1 (MQ=255)
                                                  cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGt                                                                                                >  1:2891712/1‑150 (MQ=255)
                                                   ttCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGtt                                                                                               >  2:3310663/1‑150 (MQ=255)
                                                    tCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTa                                                                                              >  1:1374621/1‑150 (MQ=255)
                                                        aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCa                                                                                          >  2:2229400/1‑150 (MQ=255)
                                                           gcagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATg                                                                                       <  2:688897/150‑1 (MQ=255)
                                                            cagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATga                                                                                      >  1:301287/1‑150 (MQ=255)
                                                             agcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                     >  2:1614683/1‑150 (MQ=255)
                                                              gcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgaga                                                                                    >  1:2498265/1‑150 (MQ=255)
                                                                 aaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaa                                                                                 >  2:280937/1‑150 (MQ=255)
                                                                 aaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaa                                                                                 <  1:2085557/150‑1 (MQ=255)
                                                                   aaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAg                                                                               >  2:2030306/1‑150 (MQ=255)
                                                                   aaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAg                                                                               <  2:403105/150‑1 (MQ=255)
                                                                     tGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGcc                                                                             <  1:6131/150‑1 (MQ=255)
                                                                      gCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCa                                                                            >  1:2602711/1‑150 (MQ=255)
                                                                        cAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAgg                                                                          >  1:470547/1‑150 (MQ=255)
                                                                        cAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAgg                                                                          <  1:429199/150‑1 (MQ=255)
                                                                         aGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGgc                                                                         >  1:35263/1‑150 (MQ=255)
                                                                          ggCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGgcg                                                                        <  2:1548218/150‑1 (MQ=255)
                                                                          ggCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGgcg                                                                        >  2:2458177/1‑150 (MQ=255)
                                                                           gCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGgcgc                                                                       >  1:3040181/1‑150 (MQ=255)
                                                                               acCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTc                                                                   >  1:1405476/1‑150 (MQ=255)
                                                                                  gagaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAgg                                                                >  1:1092038/1‑150 (MQ=255)
                                                                                       aaaaGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGagaa                                                           >  1:1705945/1‑150 (MQ=255)
                                                                                        aaaGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGagaag                                                          >  2:2102375/1‑150 (MQ=255)
                                                                                          aGCCACCATCGGCCCACCGCTGACCTCACTTTCACCGGAGATAATACTCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGAc                                                        <  2:569473/150‑1 (MQ=255)
                                                                                               ccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGAt                                                   >  2:810934/1‑150 (MQ=255)
                                                                                                caTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATa                                                  >  1:2510196/1‑150 (MQ=255)
                                                                                                 aTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATaa                                                 >  2:2485388/1‑150 (MQ=255)
                                                                                                   cGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAg                                               >  1:1041428/1‑150 (MQ=255)
                                                                                                     gCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGaa                                             <  1:3155096/150‑1 (MQ=255)
                                                                                                      cccAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAt                                            <  1:3506834/150‑1 (MQ=255)
                                                                                                         aaCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                         >  2:1858852/1‑150 (MQ=255)
                                                                                                          aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCa                                        <  2:1931076/150‑1 (MQ=255)
                                                                                                          aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCa                                        <  1:691033/150‑1 (MQ=255)
                                                                                                           cGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCaa                                       <  1:2277345/150‑1 (MQ=255)
                                                                                                            gCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAAt                                      >  2:3470532/1‑150 (MQ=255)
                                                                                                            gCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAAt                                      <  2:3325308/150‑1 (MQ=255)
                                                                                                              tGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATca                                    <  1:1939815/150‑1 (MQ=255)
                                                                                                                aCATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcacc                                  >  2:677885/1‑150 (MQ=255)
                                                                                                                aCATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcacc                                  >  2:3236124/1‑150 (MQ=255)
                                                                                                                aCATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcacc                                  >  2:1338087/1‑150 (MQ=255)
                                                                                                                   tCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCaccacc                               <  2:2897520/150‑1 (MQ=255)
                                                                                                                    cAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAccacca                              <  2:47998/150‑1 (MQ=255)
                                                                                                                    cAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAccacca                              <  2:3593368/150‑1 (MQ=255)
                                                                                                                      aTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGc                            <  2:2054027/150‑1 (MQ=255)
                                                                                                                            ccGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAcca                      <  2:3551337/150‑2 (MQ=255)
                                                                                                                              ggAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAt                    >  1:3225927/1‑150 (MQ=255)
                                                                                                                                agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtct                  <  2:2545365/150‑1 (MQ=255)
                                                                                                                                agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtct                  <  2:3346573/150‑1 (MQ=255)
                                                                                                                                  ataataATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCa                <  2:1092038/150‑1 (MQ=255)
                                                                                                                                    aagaatCAGCGTCAGAGCCCAGGCGGCAATCCCCATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCagt              <  2:303982/147‑3 (MQ=255)
                                                                                                                                     ataatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTa             <  1:1561765/150‑1 (MQ=255)
                                                                                                                                      taatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTaa            <  1:2030306/150‑1 (MQ=255)
                                                                                                                                       aatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat           <  1:2229400/150‑1 (MQ=255)
                                                                                                                                        atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAata          <  2:1405476/150‑1 (MQ=255)
                                                                                                                                        atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAata          <  1:637439/150‑1 (MQ=255)
                                                                                                                                         tCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAatat         <  1:1364100/150‑1 (MQ=255)
                                                                                                                                          cAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATg        >  2:851653/1‑150 (MQ=255)
                                                                                                                                           aGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATgg       >  2:875599/1‑150 (MQ=255)
                                                                                                                                             cGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGNg     <  1:2182842/150‑1 (MQ=255)
                                                                                                                                              gTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGa    <  1:1876970/150‑1 (MQ=255)
                                                                                                                                              gTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGa    >  1:2864632/1‑150 (MQ=255)
                                                                                                                                                cATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGACt  <  2:3664926/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGACT  >  CP009273/2669831‑2670124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑