Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 229,915 C→A M176I (ATG→ATT)  mltD ← putative membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273229,9150CA99.6% 849.6 / NA 254M176I (ATG→ATT) mltDputative membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (134/119);  total (134/120)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

CTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAA  >  CP009273/229768‑230060
                                                                                                                                                   |                                                                                                                                                 
cTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGcataat                                                                                                                                                 <  1:1894614/150‑1 (MQ=255)
       aaCGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAgc                                                                                                                                          >  2:32009/1‑150 (MQ=255)
          ggTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGcgcg                                                                                                                                       >  2:3481566/1‑150 (MQ=255)
          ggTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGcgcg                                                                                                                                       >  2:13723/1‑150 (MQ=255)
             aaCGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCa                                                                                                                                    <  2:3666846/150‑1 (MQ=255)
               cGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAgt                                                                                                                                  >  1:3322561/1‑150 (MQ=255)
                gACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAgtt                                                                                                                                 <  2:1097302/150‑1 (MQ=255)
                  ccAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAgttgt                                                                                                                               >  1:117873/1‑150 (MQ=255)
                   cAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAgttgtt                                                                                                                              <  1:2464631/150‑1 (MQ=255)
                   cAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAgttgtt                                                                                                                              <  1:2121647/150‑1 (MQ=255)
                    aGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGttgttg                                                                                                                             <  2:638321/150‑1 (MQ=255)
                    aGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGttgttg                                                                                                                             >  1:2116206/1‑150 (MQ=255)
                     gAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGa                                                                                                                            <  2:444328/150‑1 (MQ=255)
                      aaGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGaa                                                                                                                           >  2:895365/1‑150 (MQ=255)
                      aaGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGaa                                                                                                                           >  1:1529177/1‑150 (MQ=255)
                         tCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGca                                                                                                                        <  2:3446842/150‑1 (MQ=255)
                          ccGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGcaa                                                                                                                       <  2:2941956/150‑1 (MQ=255)
                              ggATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCaacaac                                                                                                                   >  2:1268228/1‑150 (MQ=255)
                              ggATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCaacaac                                                                                                                   <  2:2061445/150‑1 (MQ=255)
                                aTTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACAt                                                                                                                 <  1:716604/150‑1 (MQ=255)
                                   tCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATcgc                                                                                                              >  1:3524210/1‑150 (MQ=255)
                                    cccACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATcgcg                                                                                                             <  1:2974712/150‑1 (MQ=255)
                                    cccACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATcgcg                                                                                                             >  1:3164449/1‑150 (MQ=255)
                                       aCGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGAcg                                                                                                          >  1:3045040/1‑150 (MQ=255)
                                              ttGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCa                                                                                                   <  2:1673011/150‑1 (MQ=255)
                                               tGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCAt                                                                                                  <  1:2328007/150‑1 (MQ=255)
                                                gTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATa                                                                                                 >  2:2992858/1‑150 (MQ=255)
                                                     ttttAAGTGCCTTCATGACCCGACCGTCGCCGCTGTTATCAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATAGTCCGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGc                                                                                            <  2:3394986/150‑1 (MQ=255)
                                                      tttAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCg                                                                                           <  1:1621537/150‑1 (MQ=255)
                                                       ttAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCgg                                                                                          >  2:3130595/1‑150 (MQ=255)
                                                         aaTTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGt                                                                                        >  2:3467993/1‑150 (MQ=255)
                                                         aaTTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGt                                                                                        >  2:2490640/1‑150 (MQ=255)
                                                              ccTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGtt                                                                                   >  1:1987499/1‑150 (MQ=255)
                                                               cTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGttt                                                                                  <  1:32009/150‑1 (MQ=255)
                                                               cTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGttt                                                                                  <  1:407530/150‑1 (MQ=255)
                                                               cTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGttt                                                                                  <  1:1031774/150‑1 (MQ=255)
                                                                 tCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTTTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCa                                                                                <  1:2452066/150‑1 (MQ=255)
                                                                  cATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCaa                                                                               <  2:3704281/150‑1 (MQ=255)
                                                                  cATGACCCGACCTTCGCCGCTGGTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCACCAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCaa                                                                               >  2:1496766/1‑150 (MQ=255)
                                                                   aTGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGACGCAACAACATCGCGACGCGCGTCATCATTGCGGGTCTGTTTCaaa                                                                              >  1:2565699/1‑150 (MQ=255)
                                                                   aTGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCaaa                                                                              >  1:3473542/1‑150 (MQ=255)
                                                                   aTGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCaaa                                                                              >  1:1690608/1‑150 (MQ=255)
                                                                      aCCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCa                                                                           >  2:538855/1‑150 (MQ=255)
                                                                      aCCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCa                                                                           >  2:2444010/1‑150 (MQ=255)
                                                                       cccGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCAt                                                                          >  2:1999810/1‑150 (MQ=255)
                                                                        ccGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATa                                                                         <  1:3666377/150‑1 (MQ=255)
                                                                        ccGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATa                                                                         >  2:2233483/1‑150 (MQ=255)
                                                                         tgACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATaa                                                                        >  2:2219748/2‑150 (MQ=255)
                                                                         cGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATaa                                                                        <  1:860/150‑1 (MQ=255)
                                                                         cGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATaa                                                                        >  2:46448/1‑150 (MQ=255)
                                                                          gACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAAt                                                                       >  2:2076124/1‑150 (MQ=255)
                                                                           aCCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAAtt                                                                      <  2:2844903/150‑1 (MQ=255)
                                                                           aCCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAAtt                                                                      <  1:2039116/150‑1 (MQ=255)
                                                                           aCCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAAtt                                                                      <  1:1324376/150‑1 (MQ=255)
                                                                            ccTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTg                                                                     <  1:539785/150‑1 (MQ=255)
                                                                             cTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTgc                                                                    >  2:801253/1‑150 (MQ=255)
                                                                              ttCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTgcg                                                                   <  1:2521965/150‑1 (MQ=255)
                                                                              ttCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTgcg                                                                   <  2:1911155/150‑1 (MQ=255)
                                                                               tCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTgcgc                                                                  >  1:1053110/1‑150 (MQ=255)
                                                                                  ccgcTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGcccc                                                               <  1:2754490/150‑1 (MQ=255)
                                                                                   cgcTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGcccca                                                              <  2:721616/150‑2 (MQ=255)
                                                                                       gTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCt                                                          >  1:976939/1‑150 (MQ=255)
                                                                                       gTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCt                                                          <  1:2226184/150‑1 (MQ=255)
                                                                                        ttATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTc                                                         <  1:2724150/150‑1 (MQ=255)
                                                                                         tataAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCg                                                        >  2:241821/1‑150 (MQ=255)
                                                                                           taAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGa                                                      <  2:1529177/150‑1 (MQ=255)
                                                                                             aGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAAt                                                    <  1:493815/150‑1 (MQ=255)
                                                                                              gCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATg                                                   <  2:3758509/150‑1 (MQ=255)
                                                                                              gCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATg                                                   >  1:1935436/1‑150 (MQ=255)
                                                                                                 gCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATc                                                >  2:2762017/1‑150 (MQ=255)
                                                                                                  cTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCt                                               >  1:1576244/1‑150 (MQ=255)
                                                                                                  cTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCt                                               <  2:1595139/150‑1 (MQ=255)
                                                                                                   tACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTg                                              >  2:232067/1‑150 (MQ=255)
                                                                                                          aGAAGCCAGTAGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATg                                       >  2:3293977/1‑150 (MQ=255)
                                                                                                             aGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCt                                    >  2:2139841/1‑150 (MQ=255)
                                                                                                                cAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCg                                 <  2:2263912/150‑1 (MQ=255)
                                                                                                                cAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCg                                 >  2:785959/1‑150 (MQ=255)
                                                                                                                 aGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCgg                                >  2:1916181/1‑150 (MQ=255)
                                                                                                                 aGTCGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCgg                                >  1:565286/1‑150 (MQ=255)
                                                                                                                    cGCCGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCAt                             >  1:1271708/1‑150 (MQ=255)
                                                                                                                      ccGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCAGTg                           >  2:1225420/1‑150 (MQ=255)
                                                                                                                       cGTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTgg                          >  2:1014548/1‑150 (MQ=255)
                                                                                                                        gTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGgc                         <  2:1794785/150‑1 (MQ=255)
                                                                                                                        gTCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGgc                         >  1:758553/1‑150 (MQ=255)
                                                                                                                         tCAAACATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGgcg                        <  2:1342932/150‑1 (MQ=255)
                                                                                                                           aaaCATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGcc                      >  1:1451357/1‑150 (MQ=255)
                                                                                                                           aaaCATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGcc                      >  1:292592/1‑150 (MQ=255)
                                                                                                                            aaCATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCa                     >  1:670274/1‑150 (MQ=255)
                                                                                                                            aaCATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCa                     <  1:1836888/150‑1 (MQ=255)
                                                                                                                              cATTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGa                   >  1:12215/1‑150 (MQ=255)
                                                                                                                               aTTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGAc                  <  1:3271622/150‑1 (MQ=255)
                                                                                                                               aTTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGAc                  >  1:2189260/1‑150 (MQ=255)
                                                                                                                               aTTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGAc                  <  1:1371678/150‑1 (MQ=255)
                                                                                                                               aTTTTGTTCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCAGTGCTCGTAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGAc                  <  1:3579650/150‑1 (MQ=255)
                                                                                                                                     ttCAGACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCg            <  2:3604632/150‑1 (MQ=255)
                                                                                                                                         gACGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAg        >  1:1489839/1‑150 (MQ=255)
                                                                                                                                           cGCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGa      >  2:2794521/1‑150 (MQ=255)
                                                                                                                                            gCTGCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGAt     >  2:2533835/1‑150 (MQ=255)
                                                                                                                                               gCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGCTCaa  >  2:1642634/1‑150 (MQ=255)
                                                                                                                                               gCATAATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCaa  >  2:1731584/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGCCTTCATGACCCGACCTTCGCCGCTGTTATAAGCCGCTACGGTCAGAAGCCAGTCGCCGTCAAACATTTTGTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAA  >  CP009273/229768‑230060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑