Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,736,714 C→A R325S (CGC→AGC)  yfiN → putative membrane‑anchored diguanylate cyclase with an N‑terminal periplasmic domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,736,7140CA100.0% 700.2 / NA 209R325S (CGC→AGC) yfiNputative membrane‑anchored diguanylate cyclase with an N‑terminal periplasmic domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (107/102);  total (107/102)

Only 100 of 209 total aligned reads displayed.

GCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT  >  CP009273/2736567‑2736860
                                                                                                                                                   |                                                                                                                                                  
  ccGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCt                                                                                                                                                <  2:684928/150‑1 (MQ=255)
   cGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTg                                                                                                                                               >  2:115149/1‑150 (MQ=255)
    gAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTgg                                                                                                                                              >  1:3210656/1‑150 (MQ=255)
    gAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTgg                                                                                                                                              <  1:2656583/150‑1 (MQ=255)
    gAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTgg                                                                                                                                              >  1:2628758/1‑150 (MQ=255)
     aaaaaCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTggg                                                                                                                                             >  1:2885950/1‑150 (MQ=255)
      aaaaCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGggc                                                                                                                                            >  1:237350/1‑150 (MQ=255)
       aaaCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGggcg                                                                                                                                           >  1:451117/1‑150 (MQ=255)
        aaCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGggcgg                                                                                                                                          >  2:3671576/1‑150 (MQ=255)
          cGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGTCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGgcggcg                                                                                                                                        <  2:2018627/150‑1 (MQ=255)
          cGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGgcggcg                                                                                                                                        >  2:3294500/1‑150 (MQ=255)
             cGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATg                                                                                                                                     >  2:2241387/1‑150 (MQ=255)
                cGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTTATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAt                                                                                                                                  <  2:160913/150‑1 (MQ=255)
                cGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAt                                                                                                                                  <  2:3170841/150‑1 (MQ=255)
                 gTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAtt                                                                                                                                 <  2:1983532/150‑1 (MQ=255)
                  ttACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTc                                                                                                                                <  1:3744596/150‑1 (MQ=255)
                  ttACTATTTCTTGATGGCGAAAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTc                                                                                                                                <  2:618176/150‑1 (MQ=255)
                   tACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCg                                                                                                                               <  1:2585702/150‑1 (MQ=255)
                    aCTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGc                                                                                                                              >  2:1516618/1‑150 (MQ=255)
                      tATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTa                                                                                                                            >  1:142294/1‑150 (MQ=255)
                      tATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTa                                                                                                                            >  2:1667469/1‑150 (MQ=255)
                         ttCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATgg                                                                                                                         >  2:3046431/1‑150 (MQ=255)
                             tGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                     <  1:709010/150‑1 (MQ=255)
                               aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGctct                                                                                                                   >  1:392252/1‑150 (MQ=255)
                                 ggCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTat                                                                                                                 >  1:1209086/1‑150 (MQ=255)
                                 ggCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTat                                                                                                                 >  1:2097372/1‑150 (MQ=255)
                                  gCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatg                                                                                                                >  1:2922487/1‑150 (MQ=255)
                                    gATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgat                                                                                                              <  1:3170476/150‑1 (MQ=255)
                                      tAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGt                                                                                                            <  2:2693132/150‑1 (MQ=255)
                                       aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTa                                                                                                           >  2:2886916/1‑150 (MQ=255)
                                       aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTa                                                                                                           >  2:2655002/1‑150 (MQ=255)
                                        aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGTGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTAc                                                                                                          >  2:2361167/1‑150 (MQ=255)
                                            cAAATACATCAATGATACCTGTGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTc                                                                                                      >  1:2792208/1‑150 (MQ=255)
                                              aaTACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCgg                                                                                                    >  2:666629/1‑150 (MQ=255)
                                              aaTACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCgg                                                                                                    >  1:1877835/1‑150 (MQ=255)
                                                 aCATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAt                                                                                                 >  1:3576436/1‑150 (MQ=255)
                                                  cATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATc                                                                                                >  2:2472231/1‑150 (MQ=255)
                                                     cAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGa                                                                                             >  2:1408767/1‑150 (MQ=255)
                                                     cAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGa                                                                                             >  1:705860/1‑150 (MQ=255)
                                                        tGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGt                                                                                          <  2:2080559/150‑1 (MQ=255)
                                                          aTACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgc                                                                                        >  1:3018463/1‑150 (MQ=255)
                                                           tACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgca                                                                                       >  2:133888/1‑150 (MQ=255)
                                                           tACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgca                                                                                       >  2:2045826/1‑150 (MQ=255)
                                                           tACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgca                                                                                       <  2:2576214/150‑1 (MQ=255)
                                                              cTGGGGACATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgcagca                                                                                    >  2:2896895/1‑150 (MQ=255)
                                                                 gggTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGat                                                                                 <  1:3725238/150‑1 (MQ=255)
                                                                  ggTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGata                                                                                <  2:2234062/150‑1 (MQ=255)
                                                                    tCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATg                                                                              <  2:52884/150‑1 (MQ=255)
                                                                     cATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGt                                                                             <  1:538274/150‑1 (MQ=255)
                                                                     cATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGt                                                                             <  1:1512182/150‑1 (MQ=255)
                                                                     cATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGt                                                                             <  1:490436/150‑1 (MQ=255)
                                                                     cATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGt                                                                             <  1:1628347/150‑1 (MQ=255)
                                                                      aTGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGtt                                                                            <  1:838292/150‑1 (MQ=255)
                                                                      aTGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGtt                                                                            >  1:1288435/1‑150 (MQ=255)
                                                                      aTGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGGGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGtt                                                                            <  1:3662764/150‑1 (MQ=255)
                                                                        gCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCa                                                                          >  2:1964032/1‑150 (MQ=255)
                                                                             gggCGATAGAGTCTTGTTTGAAATCGCAAATCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACt                                                                     <  2:2710894/150‑1 (MQ=255)
                                                                                cGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                  >  1:3330878/1‑150 (MQ=255)
                                                                                cGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                  <  2:1848772/150‑1 (MQ=255)
                                                                                 gATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAATCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGaca                                                                 <  2:2377367/150‑1 (MQ=255)
                                                                                 gATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGaca                                                                 <  1:2542151/150‑1 (MQ=255)
                                                                                 gATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGaca                                                                 >  2:3350330/1‑150 (MQ=255)
                                                                                    agagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACaa                                                              >  2:3145382/1‑150 (MQ=255)
                                                                                    agagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACaa                                                              >  1:1122961/1‑150 (MQ=255)
                                                                                    agagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACaa                                                              >  1:3079772/1‑150 (MQ=255)
                                                                                      agTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAAt                                                            >  2:1160842/1‑150 (MQ=255)
                                                                                       gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATc                                                           >  1:2342034/1‑150 (MQ=255)
                                                                                          ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCttt                                                        <  2:237458/150‑1 (MQ=255)
                                                                                          ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCttt                                                        <  1:3428052/150‑1 (MQ=255)
                                                                                           tgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTa                                                       >  1:1003789/1‑150 (MQ=255)
                                                                                             attgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAt                                                     <  2:1080569/150‑1 (MQ=255)
                                                                                                  aaTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTccc                                                >  2:3539431/1‑150 (MQ=255)
                                                                                                   aTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCg                                               >  1:2812001/1‑150 (MQ=255)
                                                                                                      gCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGttt                                            <  2:3478587/150‑1 (MQ=255)
                                                                                                       cAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTg                                           >  2:1494586/1‑150 (MQ=255)
                                                                                                        aaaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGa                                          <  2:252478/150‑1 (MQ=255)
                                                                                                         aaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAt                                         >  2:3414268/1‑150 (MQ=255)
                                                                                                             ggTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTc                                     <  1:3294500/150‑1 (MQ=255)
                                                                                                             ggTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTc                                     >  2:2631055/1‑150 (MQ=255)
                                                                                                             ggTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTc                                     >  2:179446/1‑150 (MQ=255)
                                                                                                              gTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCa                                    >  2:1904709/1‑150 (MQ=255)
                                                                                                              gTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCa                                    <  1:2014337/150‑1 (MQ=255)
                                                                                                              gTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCa                                    >  2:2277209/1‑150 (MQ=255)
                                                                                                                  gCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAAt                                <  1:3350330/150‑1 (MQ=255)
                                                                                                                  gCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAAt                                <  1:914076/150‑1 (MQ=255)
                                                                                                                     gAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGc                             >  1:1733612/1‑150 (MQ=255)
                                                                                                                      aaTTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGcc                            <  1:1369468/150‑1 (MQ=255)
                                                                                                                      aaTTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGcc                            <  1:3266909/150‑1 (MQ=255)
                                                                                                                      aaTTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGcc                            <  1:1384969/150‑1 (MQ=255)
                                                                                                                        tttGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCAt                          <  1:983422/150‑1 (MQ=255)
                                                                                                                         ttGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATc                         >  2:2444384/1‑150 (MQ=255)
                                                                                                                           ggCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAg                       <  2:3183742/150‑1 (MQ=255)
                                                                                                                                 cTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGaccacc                 <  2:1222458/150‑1 (MQ=255)
                                                                                                                                  tGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGAccacca                >  1:606565/1‑150 (MQ=255)
                                                                                                                                   gCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCAt               <  2:3210656/150‑1 (MQ=255)
                                                                                                                                       cATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACa           <  1:1976424/150‑1 (MQ=255)
                                                                                                                                            aGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAg      >  1:2293871/1‑150 (MQ=255)
                                                                                                                                              cATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCa    >  1:2160360/1‑150 (MQ=255)
                                                                                                                                                tACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCAtt  <  2:1877835/150‑1 (MQ=255)
                                                                                                                                                tACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCAtt  <  1:666629/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT  >  CP009273/2736567‑2736860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑